miR-145-5p靶向TPM3通过ERK信号通路抑制甲状腺癌TPC-1细胞侵袭和转移

目的 探讨miR-145-5p对甲状腺癌细胞侵袭和转移的影响.方法 将miR-145-5p mimic质粒、mimic-NC质粒、TPM3质粒分别或联合转入甲状腺癌细胞,RT-qPCR检测miR-145-5p与TPM3 mRNA的表达,双荧光素酶报告检测靶向关系,Transwell法检测细胞侵袭能力,划痕实验检测细胞迁...     

I267L Is Neither the Virulence- Nor the Replication-Related Gene of African Swine Fever Virus and Its Deletant Is an Ideal Fluorescent-Tagged Virulence Strain

African swine fever virus (ASFV) is the causative agent of African swine fever (ASF) which reaches up to 100% case fatality in domestic pigs and wild boar and causes significant economic losses in the swine industry. Lack of knowledge of the function of ASFV genes is a serious impediment to the development of the safe and effective vaccine. Herein, I267L was identified as a relative conserved gene and an early expressed gene. A recombinant virus (SY18ΔI267L) with I267L gene deletion was produced by replacing I267L of the virulent ASFV SY18 with enhanced green fluorescent protein (EGFP) cassette. The replication kinetics of SY18ΔI267L is similar to that of the parental isolate in vitro. Moreover, the doses of 10^2.0 TCID₅₀ (n = 5) and 10^5.0 TCID₅₀ (n = 5) SY18ΔI267L caused virulent phenotype, severe clinical signs, viremia, high viral load, and mortality in domestic pigs inoculated intramuscularly as the virulent parental virus strain. Therefore, the deletion of I267L does not affect the replication or the virulence of ASFV. Utilizing the fluorescent-tagged virulence deletant can be easy to gain a visual result in related research such as the inactivation effect of some drugs, disinfectants, extracts, etc. on ASFV.     

联合检测CA19-9和CA125鉴别良恶性腹水

目的:联合检测患者血清或腹水CA125和 CA19-9,以提高对肿瘤诊断与病情判断的准确性．方法:59例腹水患者以照良、恶性分为2 组．利用蛋白质芯片技术检测两组CA125和 CA19-9含量,并进行统计学分析．结果:恶性组腹水CA125和CA19-9分别为 602．03±405．72和370．65±490．01,均明显高于良性组,差异有显著性(P<0．01);恶性组血清CA125为213．30±251．36,与良性腹水组相比,没有显著性差异(P>0．05),而血清CA19-9 的测定值为65．80±117．45,显著高于良性腹水组(P<0．05)．CA19-9检测恶性腹水的特异性、敏感性和准确性分别为66．7％、46．2％、 57．7％;CA125的特异性、敏感性和准确性分别为57．6％、61．5％和59．3％;而联合检测腹水CA19-9和CA125的特异性、敏感性和准确性分别为57．6％、84．6％和69．5％,其中敏感性明显高于单独检测CA125(P<0．05)或CA19- 9(P<0．01)．结论:联合检测CA19-9和CA125诊断恶性腹水的敏感性和准确性可提高．     

假性甲状旁腺功能减退症Ⅰa型与GNAS1基因突变的研究——附一家系报告

目的对2例假性甲状旁腺功能减退症(PHP)Ⅰa型患者及其家系成员进行临床及遗传学研究. 方法回顾性分析我科收治2例PHP Ⅰa型患者的临床资料. 结果 2例患者均有Albright遗传性骨营养不良症(AHO)的特征性表现,其父母无异常临床表现.予补钙及维生素D治疗2.5年后,患者临床症状好转,但AHO畸形无明显变化.对患者及其父母的GNAS1基因进行测序,将所有基因序列与文献报道和GenBank数据库序列进行比对验证,同时测序30位与此家系无关的个体,发现2例患儿及其母亲的GNAS1基因11号外显子密码子291上都缺失一个碱基C,造成移码突变.患者父亲和30位无关个体均无此突变.结论 GNAS1的一个新的基因移码突变通过母亲遗传导致2例患儿PHPⅠa型发病,本家系中的临床和遗传分析结果都与此相符.     

Comprehensive analysis of prognostic value and immunotherapy prospect of brain cytoplasmic RNA1 in hepatocellular carcinoma

BACKGROUND The expression of brain cytoplasmic RNA1(BCYRN1) is linked to the clinicopathology and prognosis of several types of cancers, among which hepatocellular carcinoma(HCC) is one of the most frequent types of cancer worldwide.AIM To explore the prognostic value and immunotherapeutic potential of BCYRN1 in HCC by bioinformatics and meta-analysis.METHODS Information was obtained from the Cancer Genome Atlas database. First, the correlation between BCYRN1 expression and prognosis and clinico...     

腹直肌直线套管法单机位机器人辅助腹腔镜肾输尿管切除术的疗效分析

单机位机器人辅助腹腔镜肾输尿管切除术处理输尿管远端和膀胱时难度较大。我院对32例输尿管癌患者行腹直肌直线套管法单机位机器人辅助腹腔镜下肾输尿管切除术,手术难度低,手术时间短,出血量少,手术损伤小。该手术方式是治疗输尿管癌的一种安全有效的微创手术方法。     

老年机械通气患者胃肠内营养并发症原因分析及护理对策

回顾性分析23例老年机械通气患者实施胃肠内营养发生腹胀、腹泻、便秘、反流、误吸、管道堵塞、消化道出血、高血糖、计划外拔管等并发症共计64例次,并对其原因进行分析,主要原因为肠内菌群失调、气囊充气不足、长期卧床后肠蠕动减弱以及不习惯床上排便、液体滴注速度过快、营养液中的蛋白质凝固及老年危重患者机体抵抗力降低,加上插管创伤及禁食后机体的应激改变和躁动不配合等.提出密切观察病情,加强基础护理和患者健康知识教育是防止并发症发生的关键.     

Intervertebral bridging ossification after kyphoplasty in a Parkinson’s patient with Kummell’s disease: A case report

BACKGROUNDThe short-term therapeutic efficacy of kyphoplasty on Kummell’s disease is obvious. However, postoperative refracture and adjacent vertebral fracture occur occasionally and are difficult to treat. Parkinson''s disease (PD) is a pathological disorder associated with heterotopic ossification. In a patient with PD, an intervertebral bridge was formed in a short period of time after postoperative refracture and adjacent vertebral fracture, providing new stability.CASE SUMMARYA 78-year-old woman had been suffering from PD for more than 10 years. Three months before operation, she developed lower back pain and discomfort. The visual analog scale (VAS) score was 9 points. Preoperative magnetic resonance imaging indicated collapse of the L2 vertebra. Kyphoplasty was performed and significantly decreased the severity of intractable pain. The patient’s VAS score for pain improved from 9 to 2. Fifty days postoperatively, the patient suddenly developed severe back pain, and the VAS score was 9 points. X-ray showed L2 vertebral body collapse, slight forward bone cement displacement, L1 vertebral compression fracture, and severe L1 collapse. The patient was given calcium acetate capsules 0.6 g po qd and alfacalcidol 0.5ug po qd, and bed rest and brace protection were ordered. After conservative treatment for 2 mo, the patient''s back pain was alleviated, and the VAS score improved from 9 to 2. Computed tomography at the 7-mo follow-up indicated extensive callus formation around the T12-L2 vertebrae and intervertebral bridging ossification, providing new stability.CONCLUSIONKyphoplasty is currently a conventional treatment for Kummell''s disease, with definite short-term effects. However, complications still occur in the long term, and these complications are difficult to address; thus, the treatment needs to be selected carefully. To avoid refracture, an interlaced structure of bone cement with trabeculae should be created to the greatest extent possible during the injection of bone cement. Surgical intervention may not be urgently needed when a patient with PD experiences refracture and adjacent vertebral fracture, as a strong bridge may help stabilize the vertebrae and relieve pain.     

医师工作倦怠与职业紧张和应对资源的关系评价

目的探讨医师工作倦怠与状及职业紧张、应对资源对医师工作倦怠的影响。方法从3所省市级医院随机抽取561名各科临床医师,采用MBI—GS和OSI—R对其工作倦怠、职业紧张和应对资源进行测量．结果外科、内科医师的工作倦怠高于其他临床科室（P〈0．05）;0-40岁的医师衰竭得分最高（P〈005）;业低效能感随年龄的增高而下降、随学历的增高而增高僻〈0．05）;职业紧张和应对资源的性质不同对医师工作倦怠的影响也不同,衰竭的主要预测变量有任务过重、责任感、工作环境、娱乐休闲和自我保健等（P〈0．05）,消极怠慢的主要预测变量有任务不适、任务过重和责任感等（P〈0．05）,专业低效能感的主要预测变量为任务不适、社会支持和理性处事等（P〈0．05）．结论降低或保持适度的职业任务量、职业责任感,明确职工工作要求和工作中的角色与职责,丰富其业余生活、提高其自我保健能力与理性处事能力是预防医师工作倦怠的重要措施．