Effect of FK‐506 (tacrolimus) therapy on bone healing of titanium implants: a histometric and biomechanical study in mice

Immunosuppressive agents have been recognized as a factor affecting bone metabolism. The aim of the present study was to evaluate the influence of FK‐506 administration on the healing of bone around titanium implants. Thirty‐two male mice were randomly allocated to two groups: the FK‐506 group (n = 16 mice), which received subcutaneous administration of FK‐506 (1 mg kg^?1 d^?1); and the control group (n = 16 mice), which received saline solution by the same route of administration. After 2 wk, one titanium implant with sandblasted/acid‐etched surface was placed in the femur. The therapy continued until the mice were killed 2 and 4 wk after surgery. The femurs with implants were evaluated by biomechanical testing and histologic analysis. The bone–implant contact (BIC) and bone volume (BV/TV) within a 100‐μm‐wide circumferential zone lateral to the implant surface were histomorphometrically analyzed. Compared with the control group, the FK‐506 group showed significantly lower BIC and BV/TV at both 2 and 4 wk. Biomechanical tests showed that FK‐506 significantly impaired the strength of bone–implant integration at both 2 and 4 wk postoperatively. Our data indicate that immunosuppressive therapy with FK‐506 negatively affects the fixation of titanium implants.     

Meta-Analysis: Therapeutic Effect of Transcatheter Arterial Chemoembolization Combined With Compound Kushen Injection in Hepatocellular Carcinoma

Compound Kushen Injection (CKI) is Sophora Flavescens and Heterosmilacis Japonicae extract. Meta-analysis confirmed that CKI plus transcatheter arterial chemoembolization (TACE) is more superior to TACE alone for unresectable hepatocellular carcinoma (UHCC) patients.     

Tau基因多态性与散发型进行性核上性麻痹易感性的Meta分析

OBJECTIVE: To quantitatively evaluate the association between tau genetic polymorphism (H1 and H2) and susceptibility to sporadic progressive supranuclear palsy (PSP). DATA SOURCES: Relevant Medical Subject Heading terms and text words were used to identify articles from MEDLINE (1966/2010-07), EMBASE (1984/2010-07), and Chinese National Knowledge Infrastructure (1979/2010), as well as references of the retrieved articles. STUDY SELECTION: The selected articles met the following criteria: sporadic PSP case group and healthy control group, as well as genotype frequency (H1/H1 and H1/H2 + H2/H2) in cases and controls. Genotype distribution in the control groups was tested using the Hardy-Weinberg Equilibrium (HWE). Articles irrelevant to HWE were excluded, and a forest plot was performed to combine all selected articles with Review Manager (Version 5.0). MAIN OUTCOME MEASURES: The summary odds ratios and corresponding 95% confidence intervals (95%CI) for tau polymorphism (H1/H1 and H1/H2 + H2/H2) between sporadic PSP case and healthy control groups were estimated using the fixed effects model to assess whether tau genetic polymorphism is associated with sporadic PSP susceptibility. RESULTS: According to inclusion and exclusion criteria, a total of 16 articles, which included 1 337 sporadic PSP cases and 2 073 controls, were used in the study. Two articles were excluded because of deviation from HWE in the control groups. The combined result, based on all studies, showed a significant difference in genotype distribution between cases and controls: H1H1 vs. H1H2 + H2H2 (odds ratio (OR) = 4.98, 95%CI: 3.97–6.23, P < 0.01). Stratifying for geographic distribution of PSP, sporadic PSP cases exhibited a significantly higher frequency of H1H1 genotypes than controls in the United States (OR = 4.07, 95%CI: 3.16–5.25, P < 0.01) and Europe (OR = 8.60, 95%CI: 5.05–14.64, P < 0.01). CONCLUSION: Tau genetic polymorphism is associated with sporadic PSP susceptibility, and geographic distribution might play a role in tau genetic polymorphism and sporadic PSP susceptibility.     

类风湿性关节炎患者BAFF-R水平检测及意义

目的 检测类风湿关节炎(RA)患者外周血膜结合型淋巴细胞刺激因子受体(BAFF-R)水平,并探讨其与炎症指标的相关性及临床应用价值.方法 取RA患者外周抗凝血,经过荧光标记后流式细胞仪检测BAFF-R.结果 RA患者膜结合型BAFF-R与正常人比较有统计学意义,治疗前后对比有统计学意义,且与炎症指标呈相关性.结论 RA患者膜结合型BAFF-R水平升高,可作为诊断依据及治疗靶点之一.     

Tid1 functions as a tumour suppressor in head and neck squamous cell carcinoma

Human tumourous imaginal disc (Tid1), a human homologue of the Drosophila tumour suppressor protein Tid56, is involved in multiple intracellular signalling pathways such as apoptosis, cell proliferation, and cell survival. Here, we investigated the anti‐tumourigenic activity of Tid1 in head and neck squamous cell carcinoma (HNSCC) in vitro and in vivo. Firstly, the clinical association between Tid1 expression and progression of HNSCC was explored. It was found that expression of Tid1 was negatively associated with tumour status, recurrence, and survival prognosis using immunohistochemical analysis of primary HNSCC patient tumour tissue. Secondly, ectopic expression of Tid1 in HNSCC cells was shown to significantly inhibit cell proliferation, migration, invasion, anchorage‐independent growth, and xenotransplantation tumourigenicity. Thirdly, we showed that overexpression of Tid1 attenuated EGFR activity and blocked the activation of AKT in HNSCC cells, which are known to be involved in the regulation of survival in HNSCC cells. On the other hand, ectopic expression of constitutively active AKT greatly reduced apoptosis induced by Tid1 overexpression. Together, these findings suggest that Tid1 functions as a tumour suppressor in HNSCC tumourigenesis. Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.     

重组人脑利钠肽治疗急性左心衰的临床研究

目的:观察重组人脑利钠肽(新活素)用于治疗急性左心衰的疗效.方法:53例急性左心衰老患者随机分为两组,对照组25例在常规治疗的的基础上给予应用硝酸甘油,实验组28例在常规治疗的基础上应用新活素,观察两组治疗前后的生命体征、临床症状和血流动力学指标.结果:实验组较对照组改善心功能效果更加显著.结论:新活素能够明显改善急性左心功能不全,效果确切.     

江西省山丘型血吸虫病传播控制地区野生动物血吸虫感染调查

目的　掌握江西省山丘型血吸虫病传播控制地区野生动物血吸虫感染情况，为实施精准防控措施及实现血吸虫病传播阻断和消除目标提供科学依据。方法　选择江西省血吸虫病疫情较重的山丘型流行区瑞昌市和彭泽县的5个流行村作为调查村。在调查村有螺环境捕捉野鼠等野生动物，收集来自调查村的野生动物肝脏，检查其血吸虫感染情况。在调查村采用间接血凝试验（IHA）筛查、粪便尼龙绢袋集卵孵化法和改良加藤厚涂片法检测人群血吸虫感染，采用粪便塑料杯顶管孵化法检测家畜血吸虫感染；采用系统抽样结合环境抽查法调查钉螺分布，采用环介导等温扩增技术（LAMP）检测钉螺血吸虫感染。结果　在调查村捕获、收购野鼠、黄鼠狼、野猪、麂子和野兔等野生动物或肝脏样本240只（份），其中捕获野鼠172只，血吸虫感染率为2.91%，其他野生动物未发现血吸虫感染；野鼠、麂子和野猪肝毛细线虫感染率分别为12.21%、1.96%和12.50%。调查村人群和家畜中均未发现血吸虫感染；各村平均钉螺密度在0.13～0.80只/0.1 m2，在1个村发现2只检测管钉螺有血吸虫阳性。结论　江西省山丘型传播控制地区野生动物在血吸虫病传播中的作用和潜在风险仍不可忽视；应继续加强监测，并采取有针对性防治措施，以巩固血吸虫病防治成果。     

C反应蛋白在鉴别系统性红斑狼疮活动与合并感染中的意义

目的通过分析系统性红斑狼疮(system ic lupus erythem atosus,SLE)病情活动和合并感染患者的临床和血清学特点,发现对疾病活动和感染有鉴别意义的指标。方法对2003—2004年在我科住院的110例SLE患者进行回顾性分析,比较病情活动未合并感染者87例(未感染组)和合并感染者23例(感染组)的发热、皮疹、口腔溃疡、脏器损害等临床表现,同时分析患者的C反应蛋白(CRP),血沉(ESR),血清蛋白电泳(白蛋白、α1球蛋白、α2球蛋白、β球蛋白、γ球蛋白),补体(C3、C4),免疫球蛋白(IgA、IgM、IgG)及免疫复合物(CIC)的变化。结果感染组CRP、ESR及α1球蛋白高于未感染组(P<0.01)。通过比较CRP、ESR及α1球蛋白与其他临床指标的相关性,发现CR P受感染之外的影响较少,在未感染组CRP浓度的均数在正常水平,合并感染后明显升高。感染组患者的发热、口腔溃疡、雷诺现象、心脏损害及糖皮质激素用量均高于未感染组(P<0.05)。结论譹SLE合并感染时大部分患者的CR P明显升高。譺在狼疮活动时CR P大多正常或略高,但少数患者,尤其合并浆膜炎者的CR P水平可较高。CRP对于鉴别狼疮活动与合并感染有重要的临床应用价值。