大块状生物活性玻璃陶瓷表面复合骨髓-骨松质的成骨作用研究

将自体骨髓-骨松质(ABMC)复合于块状生物活性玻璃陶瓷(BGC)表面，植入12条杂种狗无骨膜的下颌骨缺损区，分别于术后4周、12周、20周和40周处死，采用光镜、荧光显微镜及扫描电镜观察局部新骨生成与改建状况。发现术后4同时植入BGC上面有多量的新生骨形成，随时间的推移，此层骨组织增厚，并从不规则的骨芽向小梁骨转化，沉积在BGC表面或微孔内。而对照组始终末见骨形成。经结果表明BGC可以作为ABMC的生物载体，促进ABMC的异位成骨效应。BGC表面复合ABMC种植改善了BGC的力学强度．扩大了临床应用范围。     

山东地区正常He人群样本的颅面结构特征

目的本文将山东正常     

Effects of Nd:YAG and CO2 laser treatment and ultrasonic scaling on periodontal pockets of chronic periodontitis patients

BACKGROUND: The aim of the present study was to compare the effectiveness of Nd:YAG and CO2 laser treatment to that of ultrasonic scaling used as monotherapies by examining clinical parameters, subgingival microflora, and interleukin-1 beta (IL-1beta) in gingival crevicular fluid (GCF). METHODS: Eighteen patients, each of whom had 2 or more sites with probing depth measuring > 5 mm, were included this clinical trial. The 41 sites were randomly assigned treatment with either Nd:YAG laser alone (n = 14, 100 mj, 20 pps, 2.0 W, 120 seconds), CO2 laser alone (n = 13, 2.0 W, 120 seconds), or ultrasonic scaling alone (n = 14, maximum power, 120 seconds). At baseline and at 1, 4, and 12 weeks, clinical measurements (plaque index, PI; gingival index, GI; probing depth, PD; clinical attachment level, CAL; and bleeding on probing, BOP) were performed and subgingival plaque and GCF sampled. A quantitative analysis of Porphyromonas gingivalis was carried out using real-time polymerase chain reaction (PCR) procedures. The amounts of IL-1beta were estimated by an enzyme-linked immunosorbent assay (ELISA). RESULTS: Decreased inflammation and PD were observed in all 3 groups after treatment. A microbiological analysis indicated significant decreases in P. gingivalis in the Nd:YAG and scaling groups at 1, 4, and 12 weeks compared to baseline (P < 0.05). The amount of GCF significantly decreased in the Nd:YAG and scaling groups at 12 weeks. The amount of IL-1beta increased in the CO2 group from baseline to 1 week (P < 0.05). The Nd:YAG group tended to show a decrease in IL-1beta from 1 to 12 weeks, although these data were not statistically significant. CONCLUSIONS: Our data suggest that Nd:YAG laser and ultrasonic scaling treatments showed significant improvements regarding the clinical parameters and subgingival microflora compared to the baseline, but no significant difference was observed between the 3 groups.     

差速传代纯化大鼠牙囊细胞

目的:建立一种简捷的分离培养和纯化大鼠牙囊细胞的方法。方法:分离出生后6dSD大鼠上下颌第一和第二磨牙完整牙胚,剥离牙囊和成釉器,剪碎后酶消化并混合培养,再利用多次差速传代纯化牙囊细胞。结果:原代细胞为牙囊细胞和成釉器细胞混合生长,差速传代培养到第4代可获得纯化的牙囊细胞。倒置显微镜下观察牙囊细胞呈长梭形或三角形,免疫组化染色抗波形丝蛋白阳性,抗角蛋白阴性。结论:利用多次差速传代可从混合培养的原代细胞中获得纯化的牙囊细胞。     

错(牙合)患者β角的临床评价

目的推介一种新的准确评价上下颌骨矢状向关系的头影测量方法:β角.方法β角由A点、B点、髁状突中点三个骨性标志点构成,测量值反应上下颌骨在矢状方向的不调;样本选取137名患者,按照3项标准分三组,分别反应三种错(牙合)类型,并计算均数和标准差.结果安氏Ⅰ类错(牙合)β值为36.42°±3.33,安氏Ⅱ类错(牙合)的β值为26.88°±6.81,安氏Ⅲ类错(牙合)的β值为45.36°±3.68;各组β值在性别上没有显著性差异.结论β角能反应上下颌骨的矢状关系,β值在33°-39°之间为安氏Ⅰ类错(牙合)畸形,安氏Ⅱ类错(牙合)β角较锐,安氏ⅢⅡ类错(牙合)患者的β角较钝.     

Parry-Romberg syndrome combined with hemifacial spasm: association or chance event?

Parry-Romberg syndrome or progressive facial hemiatrophy is a rare clinical entity of an unknown etiology. We present the case of a 57-year-old Chinese woman with Parry-Romberg syndrome and hemifacial spasm both on the right side. Its association was not reported before. In our report of Parry-Romberg syndrome with hemifacial spasm, we try to explore whether there were some relations between the 2 diseases. We found an offending vessel compressing the root exit zone of the facial nerve and no evidence of vessel compression at ipsilateral trigeminal nerve motor rootlet.     

Synthesis and preparation of novel 4-arm star-shape poly(carboxylic acid)s for improved light-cured glass-ionomer cements.

OBJECTIVE: The objective of this study was to synthesize and characterize novel 4-arm star-shape poly(acrylic acid)s (poly(AA)s) via atom-transfer radical polymerization (ATRP) technique, tether in situ light-curable methacrylate functionalities onto the poly(AA) backbone, use these star-shape poly(AA)s to formulate the light-cured glass-ionomer cements (LCGICs), and evaluate the mechanical strengths of the formed cements. MATERIALS AND METHODS: The 4-arm poly(AA)s were synthesized using ATRP and tethered with either 2-isocyanatoethyl methacrylate (IEM) or glycidyl methacrylate (GM). The polymers were formulated with 2-hydroxyethyl methacrylte (HEMA) or methacryloyl beta-alanine (MBA), water, initiators, and Fuji II LC filler. Compressive strength (CS) was used as a tool to evaluate the formed cements. The specimens were conditioned in distilled water at 37 degrees C for 24h prior to testing. RESULTS: The 4-arm poly(AA) showed a lower viscosity as compared to its linear counterpart. Both IEM-tethered and GM-tethered 4-arm poly(AA) constructed LCGICs showed significantly high mechanical strengths. Both types of co-monomer and grafting agent dramatically affected the mechanical strengths. The MBA-containing poly(AA) cements exhibited much higher CS than the HEMA-containing cements. The IEM-tethered poly(AA) cements showed much higher CS and DTS than the GM-tethered cements. CONCLUSIONS: This study developed a novel light-curable 4-arm star-shape poly(AA) system. The system was 13% in CS, 178% in DTS and 123% in FS, compared to Fuji II LC.     

吸烟与糖尿病对龈沟液中天冬氨酸转氨酶活性水平的影响

目的 :研究吸烟、糖尿病对牙周龈沟液中天冬氨酸转氨酶 (GCF AST)水平的影响。方法 :选择 10 5例吸烟者、86例糖尿病患者及 90例对照者 ,测定牙周病指数 (PDI)、GCF AST水平并比较。结果 :吸烟组和糖尿病组PDI值均明显高于对照组 ,牙周健康区数低于对照组 ;牙龈炎区数 3组相近 ;牙周炎区数高于对照组。在PDI相同的情况下 3组GCF AST水平无显著性差异。结论 :吸烟、糖尿病是牙周病流行的高危因素 ,而对GCF AST水平无直接影响