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991.
APA微囊猪肝细胞移植治疗大鼠急性肝功能衰竭的实验研究 总被引:3,自引:1,他引:3
目的探讨APA微囊猪肝细胞移植治疗大鼠急性肝功能衰竭的有效性。方法用胶原酶灌注分离猪肝细胞,用海藻酸钠-聚赖氨酸-海藻酸钠(alginate-polylysine-alginate,APA)包埋肝细胞,测定肝细胞产量和存活率。用氨基半乳糖诱导建立大鼠急性肝功能衰竭(fulminatehepaticfailure,FHF)模型(n=68),实验动物分为微囊肝细胞植入组、游离肝细胞植入组、空囊组、对照组4组。分别将微囊肝细胞、游离肝细胞、空微囊、生理盐水植入或注入FHF大鼠腹腔,观察治疗效果。结果分离肝细胞产量为1.74×1010~2.90×1010个,微囊肝细胞存活率>80%。APA微囊肝细胞组与游离肝细胞、空囊和对照组相比,能提高FHF大鼠存活率,延长存活时间,改善肝功能。结论APA微囊猪肝细胞腹腔移植治疗FHF大鼠有较好疗效。ExperimentalStudyonHepaticFailureRatInstitute,Tianjin300170AbstractObjectiveThepaticfailurerat.Methpolylysine-alginate(APA(FHF)ratsweresetup 相似文献
992.
993.
Ischemia in isolated interventricular septa: mechanical events 总被引:2,自引:0,他引:2
994.
Positional cloning of a gene involved in hereditary multiple exostoses 总被引:21,自引:1,他引:21
Wuyts W; Van Hul W; Wauters J; Nemtsova M; Reyniers E; Van Hul EV; De Boulle K; de Vries BB; Hendrickx J; Herrygers I; Bossuyt P; Balemans W; Fransen E; Vits L; Coucke P; Nowak NJ; Shows TB; Mallet L; van den Ouweland AM; McGaughran J; Halley DJ; Willems PJ 《Human molecular genetics》1996,5(10):1547-1557
Hereditary multiple exostosis (EXT) is an autosomal dominant condition
mainly characterized by the presence of multiple exostoses on the long
bones. These exostoses are benign cartilaginous tumors (enchondromata).
Three different EXT loci on chromosomes 8q (EXT1), 11p (EXT2) and 19p
(EXT3) have been reported, and recently the EXT1 gene was identified by
positional cloning. To isolate the EXT2 gene, we constructed a contig of
yeast artificial chromosomes (YAC) and P1 clones covering the complete EXT2
candidate region on chromosome 11p11-p12. One of the transcribed sequences
isolated from this region corresponds to a novel gene with homology to the
EXT1 gene, and harbours inactivating mutations in different patients with
hereditary multiple exostoses. This indicates that this gene is the EXT2
gene. EXT2 has an open reading frame encoding 718 amino acids with an
overall homology of 30.9% with EXT1, suggesting that a family of related
genes might be responsible for the development of EXT.
相似文献
995.
Stadtmauer EA O'Neill A Goldstein LJ Crilley PA Mangan KF Ingle JN Brodsky I Martino S Lazarus HM Erban JK Sickles C Glick JH 《The New England journal of medicine》2000,342(15):1069-1076
BACKGROUND: We conducted a randomized trial in which we compared high-dose chemotherapy plus hematopoietic stem-cell rescue with a prolonged course of monthly conventional-dose chemotherapy in women with metastatic breast cancer. METHODS: Women 18 to 60 years of age who had metastatic breast cancer received four to six cycles of standard combination chemotherapy. Patients who had a complete or partial response to induction chemotherapy were then randomly assigned to receive either a single course of high doses of carboplatin, thiotepa, and cyclophosphamide plus transplantation of autologous hematopoietic stem cells or up to 24 cycles of cyclophosphamide, methotrexate, and fluorouracil in conventional doses. The primary end point was survival. RESULTS: The median follow-up was 37 months. Of 553 patients who enrolled in the study, 58 had a complete response to induction chemotherapy and 252 had a partial response. Of these, 110 patients were assigned to receive high-dose chemotherapy plus hematopoietic stem cells and 89 were assigned to receive conventional-dose chemotherapy. In an intention-to-treat analysis, we found no significant difference in survival overall at three years between the two treatment groups (32 percent in the transplantation group and 38 percent in the conventional-chemotherapy group). There was no significant difference between the two treatments in the median time to progression of the disease (9.6 months for high-dose chemotherapy plus hematopoietic stem cells and 9.0 months for conventional-dose chemotherapy). CONCLUSIONS: As compared with maintenance chemotherapy in conventional doses, high-dose chemotherapy plus autologous stem-cell transplantation soon after the induction of a complete or partial remission with conventional-dose chemotherapy does not improve survival in women with metastatic breast cancer. 相似文献
996.
Identification of a novel sarcoglycan gene at 5q33 encoding a sarcolemmal 35 kDa glycoprotein 总被引:9,自引:5,他引:9
997.
998.
In the last few years, molecular genetics analyses have permitted novel
insights into psoriasis, a disease characterized by uncontrolled
proliferation of keratinocytes and recruitment of T cells into the skin.
The disease affects approximately 1-2% of the Caucasian population and can
occur in association with other inflammatory diseases such as Crohn's
disease and in association with human immunodeficiency virus (HIV)
infection. Given that psoriasis has characteristics of an autoimmune
disease, it is not surprising that HLA studies revealed an association with
certain alleles, notably HLA-Cw6. Despite this HLA component, psoriasis in
some families is inherited as an autosomal dominant trait with high
penetrance. Loci at chromosome 17q25 and 4q have been identified following
genome-wide linkage scans of large, multiply affected families. In the case
of at least the susceptibility locus at 17q25, the development of psoriasis
does not require the presence of HLA-Cw6. Sib-pair analyses have confirmed
the association with HLA-Cw6, confirmed the existence of a locus at 17q25
and identified other possible susceptibility loci. Two independent groups
have reported a third region on chromosome 20p. Despite these findings, the
extent of genetic heterogeneity and the role of environmental triggers and
modifier genes is still not clear. The precise role of HLA also still needs
to be defined. The isolation of novel susceptibility genes will provide
insights into the precise biochemical pathways that control this disease.
Such pathways will also reveal additional candidate genes that can be
tested for molecular alterations resulting in disease susceptibility.
相似文献
999.
Croxatto HB; Kovacs L; Massai R; Resch BA; Fuentealba B; Salvatierra AM; Croxatto HD; Zalanyi S; Viski S; Krenacs L 《Human reproduction (Oxford, England)》1998,13(4):793-798
Low-dose antiprogestin administration has been proposed as a new
contraceptive modality to interference with endometrial receptivity without
disturbing ovarian function. The effects of 1 mg/day mifepristone for 150
days on the menstrual cycle were assessed in 21 surgically sterilized
women. The aim was to study each woman for one control cycle and during
months 1, 3 and 5 of treatment. Ovulation, endometrial thickness, serum
oestradiol and progesterone, urinary luteinizing hormone, endometrial
morphology and cervical mucus were assessed. Luteal phase progesterone
concentrations were observed in 36 of the 60 treated months assessed and
less frequently as treatment progressed. The bleeding pattern was regular
in most biphasic cycles, while prolonged interbleeding intervals or no
bleeding were associated with monophasic cycles. Altered endometrial
morphology was found in all cases irrespective of the occurrence of luteal
activity. Increased endometrial thickness and dilated glands were observed
in 25 and 34% respectively of the monophasic cycles. Mifepristone, 1
mg/day, interferes with endometrial development while allowing the
occurrence of biphasic ovarian cycles and regular bleeding. However, it
also prevents ovarian cyclicity in a high proportion of treated months, and
this is associated with increased endometrial growth in some women, which
may be of concern.
相似文献
1000.