缝线锚钉治疗膝关节内侧副韧带附丽点损伤

膝关节内侧副韧带(medial collateral ligament,MCL)损伤临床常见,小腿外展使膝关节突然外翻町引起MCL损伤,多见于高能量的交通伤所致的膝关节多发韧带损伤中.     

静吸复合全麻用于经DSA治疗颅内动脉瘤患者的麻醉

现将本院2006年1月-2006年12月54例经DSA全脑造影及栓塞术的麻醉处理总结如下。 1资料与方法 1．1一般资料 本组54例患者中，男29例，女25例，年龄11-70岁，ASAⅡ-Ⅲ级，急诊11例。术前昏迷5例，术中动脉瘤破裂2例。     

2001年与2004年抗菌药物应用情况比较

目的　了解抗菌药物应用的基本情况。方法　将 2 0 0 1年 3月 14 3 0例住院患者病历与 2 0 0 4年 3月的 2 0 66例住院患者病历进行回顾性调查和对比分析。结果　 2 0 0 1年的 14 3 0例中有 948(66 3 % )例 ,和 2 0 0 4年的 2 0 66例有 10 60例 (5 1.3 % ) ,应用了抗菌药物。结论　定期检查考评 ,严格掌握用药指征 ,是加强抗菌药物应用管理的关键。     

Fluorescence and photobleaching dynamics of single light-harvesting complexes

Single light-harvesting complexes LH-2 from Rhodopseudomonas acidophila were immobilized on various charged surfaces under physiological conditions. Polarized light experiments showed that the complexes were situated on the surface as nearly upright cylinders. Their fluorescence lifetimes and photobleaching properties were obtained by using a confocal fluorescence microscope with picosecond time resolution. Initially all molecules fluoresced with a lifetime of 1 ± 0.2 ns, similar to the bulk value. The photobleaching of one bacteriochlorophyll molecule from the 18-member assembly caused the fluorescence to switch off completely, because of trapping of the mobile excitations by energy transfer. This process was linear in light intensity. On continued irradiation the fluorescence often reappeared, but all molecules did not show the same behavior. Some LH-2 complexes displayed a variation of their quantum yields that was attributed to photoinduced confinement of the excited states and thereby a diminution of the superradiance. Others showed much shorter lifetimes caused by excitation energy traps that are only ≈3% efficient. On repeated excitation some molecules entered a noisy state where the fluorescence switched on and off with a correlation time of ≈0.1 s. About 490 molecules were examined.     

Quantitative Analysis of the Tomographic Technetium－99m MIBI （~（99m）Tc－MIBI） Myocardial Bullseye Display： Application to Diag

ＱｕａｎｔｉｔａｔｉｖｅＡｎａｌｙｓｉｓｏｆｔｈｅＴｏｍｏｇｒａｐｈｉｃＴｅｃｈｎｅｔｉｕｍ－９９ｍＭＩＢＩ（~（９９ｍ）Ｔｃ－ＭＩＢＩ）ＭｙｏｃａｒｄｉａｌＢｕｌｌｓｅｙｅＤｉｓｐｌａｙ：ＡｐｐｌｉｃａｔｉｏｎｔｏＤｉａｇｎｏｓｉｓｏｆＣｏｒｏｎａ?..     

Effects of dauricine,quinidine,and sotalol on action potential duration of papillary muscles in vitro

目的：观察蝙蝠葛碱对豚鼠乳头状肌的动作电位是否具有使用依赖性特征，并与奎尼丁、索他洛尔作平行比较．方法：利用细胞内微电极技术记录豚鼠乳头状肌跨膜动作电位．结果：蝙蝠葛碱２０μｍｏｌ·Ｌ－１能明显延长乳头状肌跨膜动作电位时程，在刺激周长为２００，３００，４００，６００，８００，１０００，２０００ｍｓ时，其延长动作电位时程的百分率分别为２２±８，１１±６，９±５，７±５，６±３，４３±２８，４５±２８，蝙蝠葛碱延长动作电位时程作用于刺激周期较短时作用明显，呈使用依赖性特征．而奎尼丁１μｍｏｌ·Ｌ－１和索他洛尔１０μｍｏｌ·Ｌ－１延长动作电位时程作用随刺激周期延长而增强，呈现逆向使用依赖性特征．结论：蝙蝠葛碱使用依赖性延长豚鼠乳头状肌动作电位时程．     

大鼠实验性肝硬化时甲状腺激素水平及甲状腺形态学变化的研究

将Wistar大鼠分为三组：肝硬化组，给予四氯化碳等复合因素，给药组，除给CCl4等复合因素外，用赤栀黄合剂灌胃41天；正常组给以一般饮食及饮水。结果，与正常组及给药组相比，硬化组血T3、T4测定值低，甲状腺滤泡中胶质明显减少，滤泡细胞与胶质间空泡增多，滤泡上皮细胞内PAS阳性小滴数日增多。表明在实验性肝硬化期间甲状腺参与了抗疾病的代谢活动，其机理可能是肝硬化时肝细胞合成、转运T3，T4的蛋白质减少。运输中的T3，T4减少，活性甲状腺素不足，从而导致甲状腺代偿性功能活跃。赤栀黄合剂具有保肝，恢复甲状腺素水平的作用。     

Construction of an Rb gene expression plasmid.

We obtained an 844 bp Bg1II fragment from an Rb cDNA clone and inserted it into the expression vector pWR-13 to construct an Rb gene expression plasmid. When the Rb Bg1II fragment was fused in-frame into pWR-13, it was operated by a Lac Z promoter and produced a fusion protein which consisted of expressed Rb protein and a small peptide from Lac Z. The recombinants were transformed into E. coli with the CaCl2 method, screened by in situ hybridization, and restriction mapped. Total cellular protein of transformed clones was analyzed by SDS-PAGE and Commassie blue staining. The sense clones showed a unique band at 28,000. On Western blot, this band specifically reacted with 125I-labelled antibody against synthetic Rb peptide. This protein comprised more than 5% of total bacterial protein.     

氯丙醇对大鼠的毒性研究

进行氯丙醇对大鼠毒作用研究 ,并进行氯丙醇暴露对健康影响评价的生物效应标志物探讨 ,为进行人群氯丙醇暴露对健康的影响评价提供科学依据。选用健康雄性断乳SD大鼠 1 76只 ,随机分为 8组 ,经口灌胃给予 0、0 2 5、0 5、1 0、2 0、4 0、8 0、1 6 0mg kg的 3 氯 1 ,2 丙二醇 (3 MCDP) 90天 ,进行体重、食物利用率、血液学指标、血生化指标、尿液中N 乙酰 β D 氨基葡萄糖苷酶 (NAG)、γ 谷胺酰转肽酶 (GGT)和总蛋白、精子数目、精子存活率和畸形率、睾丸组织中乳酸脱氢酶 (LDH)和乳酸脱氢酶同工酶 X(LDH X)、脏体比及其病理组织学测定和分析。结果表明 ,不同暴露剂量的氯丙醇对动物体重、食物利用率、血红蛋白、红细胞、白细胞、血丙氨酸氨基转移酶 (AST)、天冬氨酸氨基转移酶 (ALT)、肌酐、尿素氮、血清碱性磷酸酶 (ALP)、乳酸脱氢酶 (LDH)、总蛋白、白蛋白、尿GGT酶和总蛋白及睾丸LDH酶均未见显著影响。在 4 0、8 0和 1 6 0mg kg剂量组 ,动物尿N 乙酰 β D 氨基葡萄糖苷酶 (NAG)活性显著增加 ,肾体比增大 ,并出现肾毒性病理改变 ,精子数目也降低。在 8 0和 1 6 0mg kg剂量组 ,精子存活率和睾丸LDH X显著降低 ,睾丸和附睾出现病理改变 ,3 MCDP无致精子畸变作用。由此可见尿液中NAG酶活性和精子     

肾移植患者术前抗体水平的分析

对肾移植术前患者血清４５９份进行了抗体水平的分析。发现４０７例首次移植前的患者血清中８４例（２０．６％）有抗淋巴细胞抗体。再次移植前５２例中３９例（７５％）有抗淋巴细胞抗体。在阳性患者血清中对全淋巴细胞有抗体的占６８．３％，而仅对Ｂ淋巴细胞有抗体的占３１．７％。在性别上，女性产生抗体阳性率均高于男性。结果表明，初次移植肾患者输血和妊娠是致敏的重要因素，再次移植的患者移植物刺激是致敏的重要因素之一。Ｂ淋巴细胞抗体在移植物排斥的关系中可能具有保护移植物的作用。