肺栓塞1例

[背景 ]对肺栓塞的认识普遍较低 ,容易漏诊、误诊 .[病例报告 ]患者年龄为 5 7岁 ,女性 ,以原因不明的呼吸困难为主要症状 ,超声心动图检查示右心房右心室扩大及肺动脉高压 .进一步做CT肺血管造影检查确诊为肺栓塞 .[讨论 ]可根据临床表现及非特异性检查 (胸部X线片、超声波及心电图等检查 )确诊肺栓塞 ,当疑似为肺栓塞时可做有确诊意义的CT肺血管造影检查 .     

Diagnosis and characterization of non-Hodgkin's lymphoma in a patient with acute renal failure

This case illustrates the relatively rare occurrence of malignant lymphoma presenting as acute renal failure due to renal parenchymal infiltration. To our knowledge, it is the first report in which the phenotype of a non-Hodgkin's lymphoma was established using renal biopsy tissue. The dangers of treatment of renal lymphoma in the patient whose disease has not been adequately characterized have been noted by Coggins.     

含链霉素短程化疗方案的痰结核菌转阴时间观察

目的对比短程化疗含链霉素(SM)、乙胺丁醇(EMB)两组方案在抗结核治疗中的效果。方法选初治菌阳肺结核100例,予含SM方案(2HRZS/7HR)化疗50例,含EMB方案(2HRZE/7HR)化疗50例。每月检查痰结核菌一次(涂片三次,培养一次)。停药后继续追踪两年。结果痰菌2个月内转阴者,SM组29例,占63%;EMB组18例,占38%,p<0.05,有显著性差异。需延长强化期者:SM组16例,占35%;EMB组26例,占55%,p<0.05,有显著性差异。复发病例,SM组2例,占4%;EMB组3例,占6%,p>0.05,无显著性差异。结论使用含SM方案化疗的病例其痰菌转阴时间比含EMB方案短。     

艾芬地尔干预后氯化锂-匹罗卡品致(癎)大鼠的慢性期脑电图特征

目的:了解艾芬地尔干预对氯化锂(LiCl)-匹罗卡品(Pilo)致(癎)大鼠脑电图随时间变化的特征.方法:通过腹腔注射LiCl-Pilo建立癫(癎)动物模型.60只雄性Wistar大鼠分为对照组、模型组和艾芬地尔干预组,分别在造模后第7、15、30、60天四个时间点观察各组大鼠行为、脑电图改变,每一个时间点5只大鼠.结果:对照组无NFDA1性发作,经过4-20天的潜伏期后,干预组及模型组的脑电图波幅及频率骤然减低,在慢性期干预组较模型组的波幅逐渐增高,频率逐渐增快,但仍未达发作当时的水平,且两组间比较差异无统计学意义.干预组较模型组更早更快趋于正常化.结论:艾芬地尔在LiCl-Pilo致(癎)大鼠模型中具有抗惊厥作用,慢性期脑电图有特征性改变.     

生物工程技术制备人源抗-HBs Fab 片段

目的：用生物工程技术制备人源性抗-HBsFab。方法：将从抗体文库中筛选出的人源抗-HBsFab基因克隆入pBAD/gⅢA载体，进而转化Tpo10大肠杆菌，对重组质粒菌发酵表达后，利用Ni-NTA-Agarose螯合层析柱纯化周质腔可溶性Fab蛋白。对所得包涵体依次变性，溶解，纯化后，利用透析进行复性，用Western blot检测Fab蛋白的特异性，Dot blot测定其生物学活性。结果：经Ni-NTA-Agarose柱纯化的周质腔可溶性Fab蛋白，有较好的生物学活性，并且总量达到80mg/L。对所获包涵体进行透析复性后，也可得到少量有活性的蛋白，但比例很小。结论；用pBAD/gⅢA-Top10表达系统表达人源抗-HBsFab片段，发酵培养后，经有效纯化可得到生物学活性较好的可溶性蛋白。为人源抗-HBsFab片段的大量制备提供了有效手段。     

树突状细胞在胆囊癌组织内浸润的研究

目的通过对胆囊癌组织中树突状细胞浸润情况的研究,阐述树突状细胞与肿瘤免疫之间的关系。方法采用免疫组化SP法。结果树突状细胞在胆囊癌组织的浸润程度与年龄、性别和病理组织学类型无关,与病理分化程度呈负相关关系(P<005)。结论树突状细胞在胆囊癌组织的浸润程度与病理分化程度、Nevin分期、肝浸润及淋巴结转移存在密切的关系。树突状细胞的定量检测可作为估计胆囊癌预后的标志。     

Mass spectrometric identification of mtb81, a novel serological marker for tuberculosis

We have used serological proteome analysis in conjunction with tandem mass spectrometry to identify and sequence a novel protein, Mtb81, which may be useful for the diagnosis of tuberculosis (TB), especially for patients coinfected with human immunodeficiency virus (HIV). Recombinant Mtb81 was tested by an enzyme-linked immunosorbent assay to detect antibodies in 25 of 27 TB patients (92%) seropositive for HIV as well as in 38 of 67 individuals (57%) who were TB positive alone. No reactivity was observed in 11 of 11 individuals (100%) who were HIV seropositive alone. In addition, neither sera from purified protein derivative (PPD)-negative (0 of 29) nor sera from healthy (0 of 45) blood donors tested positive with Mtb81. Only 2 of 57 of PPD-positive individuals tested positive with Mtb81. Sera from individuals with smear-positive TB and seropositive for HIV but who had tested negative for TB in the 38-kDa antigen immunodiagnostic assay were also tested for reactivity against Mtb81, as were sera from individuals with lung cancer and pneumonia. Mtb81 reacted with 26 of 37 HIV(+) TB(+) sera (70%) in this group, compared to 2 of 37 (5%) that reacted with the 38-kDa antigen. Together, these results demonstrate that Mtb81 may be a promising complementary antigen for the serodiagnosis of TB.     

Expression of the 5.1 H11 antigen, a fetal muscle surface antigen, in normal and neoplastic tissue

The monoclonal antibody 5.1 H11 recognizes an antigen on human fetal muscle and on rhabdomyosarcoma cell lines and xenografts that has been shown to be homologous to the neural cell adhesion molecule. To evaluate its range of expression, we used immunoperoxidase staining of fresh frozen-tissue sections to determine monoclonal antibody 5.1 H11 reactivity in normal and neoplastic tissue. Among normal tissue specimens, intense antibody staining was seen in brain and peripheral nerve, and weaker staining in ganglionic elements of colon. In addition to 26 of 29 rhabdomyosarcoma specimens, 5.1 H11 antibody showed reactivity to 9 of 10 Wilms' tumors, 6 of 6 neural tumors, and 4 of 4 gliomas, and with single specimens of ectomesenchymoma, clear-cell sarcoma of kidney, undifferentiated sarcoma of liver, ovarian fibroma, and neurofibroma. We conclude that the monoclonal antibody 5.1 H11 recognizes an antigen present not only on fetal muscle but on normal brain and nerve as well. In addition to rhabdomyosarcoma, a variety of other tumors, most of which have been previously shown to express neural cell adhesion molecule, also appear to express the antigen recognized by 5.1 H11. Our results thus offer additional confirmatory evidence that an epitope of neural cell adhesion molecule is the antigen for 5.1 H11.