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Shuhei Kimura Yuki Morizane Ryo Matoba Mio Hosokawa Yusuke Shiode Masayuki Hirano Shinichiro Doi Shinji Toshima Kosuke Takahashi Mika Hosogi Atsushi Fujiwara Fumio Shiraga 《Japanese journal of ophthalmology》2017,61(6):472-478
Purpose
To investigate the effectiveness of displacement of submacular hemorrhage (SMH) caused by polypoidal choroidal vasculopathy (PCV) by assessing retinal sensitivity using microperimetry.Methods
We retrospectively reviewed the medical records of 11 consecutive PCV patients with SMH. All patients underwent vitrectomy, subretinal injection of tissue plasminogen activator, and fluid-air exchange, followed by antivascular endothelial growth factor therapy using a pro re nata regimen. The retinal sensitivity was measured by use of microperimetry before and after surgery.Results
The mean (SD) age of the patients was 74.1 ± 9.4 years. The mean SMH diameter was 6.8 ± 5.2 disc diameters. The best-corrected visual acuity (BCVA), mean retinal sensitivity, and mean number of measure points with a sensitivity ≥10 dB before the surgery were 0.94 ± 0.49, 4.2 ± 4.5 dB, and 15.6 ± 15.1 points, respectively. These had significantly improved 6 months after surgery (0.39 ± 0.37, 15.6 ± 7.3 dB, and 50.9 ± 22.2 points, respectively; P < 0.05 for all outcome measures). The mean number of measure points with an absolute scotoma before surgery had decreased significantly 6 months after surgery (from 40.5 ± 15.0 to 9.4 ± 16.0 points; P < 0.001).Conclusions
Displacement of SMH effectively improves retinal sensitivity as well as BCVA.84.
Porphyromonas gingivalis is a gram-negative anaerobic bacterial species implicated as an important pathogen in the development of adult periodontitis. Hemagglutinin may mediate the adsorption and invasion of bacteria into host cells. Furthermore, the hemagglutinin plays a role in the agglutinate and lyse erythrocytes intake of heme which is an absolute requirement for this bacterial growth. We previously cloned the gene encoding the 130-kDa hemagglutinin protein domain (130-kDa HMGD) and identified the functional motifs of agglutination of erythrocytes. Bacterial cell attachment to erythrocytes is an important initial step in the expression of hemolytic activity. In this study, we highly purified recombinant r130-kDa HMGD and prepared the specific antiserum. Further, the effect of the antibody on the hemolytic activity of P. gingivalis cells was examined. The polyclonal antibody recognized 43,49-kDa major bands in P. gingivalis cells and r130-kDa HMGD, and significantly inhibited the hemagglutinating and hemolytic activities of P. gingivalis cells. The findings suggest that the antibody may be useful in the development of the passive immunization against periodontal diseases caused by P. gingivalis infection. 相似文献
85.
Detection of Serum p53 Antibodies in Mucosal Esophageal Cancer and Negative Conversion After Treatment 总被引:1,自引:0,他引:1
Hideaki Shimada M.D. Miwako Arima M.D. Kazuaki Nakajima M.D. Yoshio Koide M.D. Shin-ichi Okazumi M.D. Hisahiro Matsubara M.D. Yukimasa Miyazawa M.D. Akihiko Takeda M.D. Hideki Hayashi M.D. Takehiko Yoshida M.D. Takenori Ochiai M.D. Kaichi Isono M.D. 《The American journal of gastroenterology》1998,93(8):1388-1389
86.
Sugita Chihiro Yamashita Atsushi Tsutsumi Shigetoshi Kai Hisahiro Sonoda Tohru Yoshida Hiroki Yamamoto Ryuichi Asada Yujiro Kurokawa Masahiko 《Journal of natural medicines》2021,75(4):975-984
Journal of Natural Medicines - Brazilian propolis (AF-08) is a dietary supplement containing a variety of flavonoids. It is used worldwide as a folk medicine. Flavonoids and a diet of fruits and... 相似文献
87.
Yasunori Matsumoto Masayuki Kano Kentaro Murakami Takeshi Toyozumi Hiroshi Suito Masahiko Takahashi Nobufumi Sekino Tadashi Shiraishi Toshiki Kamata Takahiro Ryuzaki Soichiro Hirasawa Kazuya Kinoshita Hisahiro Matsubara 《Cancer science》2020,111(12):4348
Our laboratory previously reported the usefulness as biomarkers of exosomes in the plasma of esophageal squamous cell carcinoma (ESCC) patients. However, the influence of tumor‐derived exosomes on the tumor itself and underlying mechanisms remain unclear. We here report changes in the phenotype and gene expression when cancer cells exist in an environment with tumor‐derived exosomes. The exosomes were isolated from the culture medium of human ESCC cells (TE2, T.Tn) by ultracentrifugation; cell proliferation assay, wound‐healing assay, and fluorescence imaging of the cell cycle were performed to clarify the phenotypic changes in the high concentration of tumor‐derived exosomes. Gene expression changes were also assessed by mRNA microarray, and the data were analyzed by gene set enrichment analysis (GSEA). The data revealed that the proliferation of both TE2 and T.Tn was inhibited, and cell migration ability was upregulated in the exosome exposure group (P < .05). Fluorescence imaging using a fluorescent ubiquitination‐based cell cycle indicator expressing ESCC cells revealed that the ratio of G1‐phase cells was significantly increased in the exosome exposure group (P < .05). Findings of the GSEA clarified that high‐density exposure of cancer‐derived exosomes to their parent cancer cells downregulated the expression of genes related to cell proliferation and cell cycle, and upregulated the expression of genes related to actin filament length and extracellular structure organization. In conclusion, an environment of high‐density tumor‐derived exosomes induces changes in the gene expression and phenotype of tumor cells and may lead to tumor progression or malignant transformation. 相似文献
88.
89.
Takahiro Kunisada Shu-Zhuang Lu Hisahiro Yoshida Satomi Nishikawa Shin-ichi Nishikawa Masako Mizoguchi Shin-ichi Hayashi Lynda Tyrrell David A. Williams Xiaomei Wang B. Jack Longley 《The Journal of experimental medicine》1998,187(10):1565-1573
The growth and differentiation of mast cells and melanocytes require stem cell factor (SCF), the ligand for the kit receptor tyrosine kinase. SCF may exist as a membrane-bound or soluble molecule. Abnormalities of the SCF-kit signaling pathway, with increased local concentrations of soluble SCF, have been implicated in the pathogenesis of the human disease cutaneous mastocytosis, but have not yet been shown to play a causal role. To investigate both the potential of SCF to cause mastocytosis and its role in epidermal melanocyte homeostasis, we targeted the expression of SCF to epidermal keratinocytes in mice with two different transgenes controlled by the human keratin 14 promoter. The transgenes contained cDNAs that either produced SCF, which can exist in both membrane-bound and soluble forms, or SCF, which remains essentially membrane bound. Murine epidermal keratinocyte expression of membrane-bound/ soluble SCF reproduced the phenotype of human cutaneous mastocytosis, with dermal mast cell infiltrates and epidermal hyperpigmentation, and caused the maintenance of a population of melanocytes in the interadnexal epidermis, an area where melanocytes and melanin are found in human skin but where they are not typically found in murine skin. Expression of membrane-bound SCF alone resulted in epidermal melanocytosis and melanin production, but did not by itself cause mastocytosis. We conclude, first, that a phenotype matching that of human mastocytosis can be produced in mice by keratinocyte overproduction of soluble SCF, suggesting a potential cause of this disease. Second, we conclude that keratinocyte expression of membrane-bound SCF results in the postnatal maintenance of epidermal melanocytes in mice. Since the resulting animals have skin that more closely approximates human skin than do normal mice, their study may be more relevant to human melanocyte biology than the study of skin of normal mice. 相似文献
90.
Shusuke Haruta Hisashi Shinohara Hisahiro Hosogi Yu Ohkura Nao Kobayashi Aya Mizuno Ryosuke Okamura Masaki Ueno Yoshiharu Sakai Harushi Udagawa 《Gastric cancer》2017,20(3):528-535