Stimulation of eosinophil production in vitro by eosinophilopoietin and spleen-cell-derived eosinophil growth-stimulating factor

Eosinophilopoietin (EPP) was previously characterized by the ability to stimulate eosinophil production in vivo, but these studies could not ascertain whether EPP had a direct effect on the bone marrow or acted indirectly by causing release of eosinophilopoietic activity by other tissues. The present studies demonstrate that EPP stimulates eosinophil growth in liquid culture of mouse bone marrow in vitro. The timing of stimulation by EPP in vivo and in vitro were parallel, with maximal eosinophil growth after 48 hr. Moreover, EPP appears similar to, and possible identical with, the eosinophil growth-stimulating substance (EO-GSF) released by antigenic stimulation of immune nonadherent spleen cells. Both EPP and EO-GSF are of low molecular weight, both produce stimulation of eosinophil growth with identical kinetics, and both produced similar dose-response curves in the liquid culture system.     

Supporting students with disability and health issues: lowering the social barriers

Medical Education 2012: 46: 564–574 Context Legislation and General Medical Council guidance require medical schools to make reasonable adjustments to enable students with disabilities to progress in their studies without disadvantage. Current definitions of disability have moved from the traditional ‘medical’ model to a ‘social’ model that emphasises the role of institutions in removing unnecessary barriers to learning through flexible provision. In response, two medical schools introduced a Student Support Card to empower students with disabilities and health issues to request reasonable adjustments. A study was carried out to ascertain the effectiveness of the Student Support Card from the user’s perspective in both institutions. Methods A questionnaire survey of card‐holders was conducted in 2009–2010. A total of 31 respondents took part, from among whom six volunteers were recruited to participate in semi‐structured interviews. Thematic analysis was conducted and data independently analysed by two researchers. Results The results from the questionnaire indicated that the scheme was well received by students and brought clear benefits, and that the card’s value lay in ownership, as well as in use. Further themes emerged from the interviews which suggested that students were judicious in requesting adjustments, thus reflecting their concerns over the disclosure of disability, the threat that their behaviour might be misinterpreted and their acquiring of the competencies necessary to become a doctor. Conclusions The benefits of such a scheme are that it is flexible and addresses unique student needs. Further research needs to focus upon: how educators perceive the utility of the Student Support Card; whether it should be embedded in the wider medical school culture, and, subsequently, how a working consensus can be achieved with regard to reasonable adjustments within vocational education in which there are clear requirements for competence.     

Preoperative radiation therapy with sensitizers in the management of carcinoma of the rectum

There is enough evidence to show that adjuvant radiation therapy contributes to the management of patients with carcinoma of the rectum. In an effort to improve resectability and possibly survival rates, the use of chemosensitizers, combined with moderate doses of radiation used presurgically, was introduced for carcinomas larger than 5 cm in diameter requiring abdominoperineal resection. Based on our experience and that of others, it is believed that the method of administration of 5FU and mitomycin-C is an important factor in obtaining an increased therapeutic ratio. Because of the locoregional pattern of spread of rectal cancer, this adjuvant approach would appear suitable. A series of approximately 60 patients is discussed and the surgical findings and five-year survival is reported. Read at the Joint Meeting of the American Society of Colon and Rectal Surgeons with the Section of Colo-Proctology, Royal Society of Medicine, and the Section of Colonic and Rectal Surgery, Royal Australasian College of Surgeons, New Orleans, Louisiana, May 6 to 11, 1984.     

Shape response of human erythrocytes to altered cell pH

Alteration of red blood cell (RBC) pH produces stomatocytosis (at low pH) and echinocytosis (at high pH). Cell shrinkage potentiates high pH echinocytosis, but shrinkage alone does not cause echinocytosis. Mechanisms for these shape changes have not been described. In this study, measured dependence of RBC shape on cell pH was nonlinear, with a broad pH range in which normal discoid shape was maintained. Transbilayer distribution of phosphatidylcholine and phosphatidylserine, measured by back-extraction of radiolabeled lipid, was the same in control and altered pH cells. Possible roles of pH- titratable inner monolayer phospholipids were examined by assessing pH- dependent shape in cells in which their levels had been perturbed. In metabolically depleted cells and calcium-treated cells, which have altered levels of phosphatidic acid, phosphatidylinositol-4-phosphate, and/or phosphatidylinositol-4,5-bisphosphate, low cell pH was stomatocytogenic and high cell pH was echinocytogenic, as in control cells. Thus, neither change in membrane lipid asymmetry nor normal levels of the pH-titratable inner monolayer lipids is necessary for cell pH-mediated shape change.     

Measuring Social Support in Pregnancy: Can It Be Simple and Meaningful?

Background: It is important to determine the level of a woman's social support at the booking‐in interview for prenatal care, but measurement tends to be ad hoc and nonquantifiable. The purpose of this study was to describe the Maternity Social Support Scale and the relationship between support scale ratings and the Edinburgh Postnatal Depression Scale and other health and service use outcomes. Methods: Women (n= 901) who attended the antenatal clinic at the Royal Women's Hospital in Brisbane, Australia, were asked to complete a support scale as part of their booking‐in interview. Participants were contacted at 16 weeks postpartum and invited to complete a follow‐up questionnaire. Relationships between the scale and study outcomes were explored using analysis of variance and chi‐square tests. Results: Women with low social support in pregnancy were more likely than well‐supported women to report poorer health during pregnancy (p= 0.006) and postnatally (p < 0.001), to book later for prenatal care (p= 0.000), to seek medical help more frequently (p= 0.004), and to be more depressed postnatally(p= 0.0001). Conclusion: Social support during pregnancy can be measured in a meaningful and simple way through the use of a short questionnaire administered at the prenatal booking‐in visit.     

麦冬多糖对正常和实验性糖尿病小鼠血糖的影响

目的：研究麦冬多糖对正常和实验性糖尿病小鼠血糖的影响。方法：昆明种小鼠４０只，分成４组，分别用麦冬多糖（１００和３００ｍｇ／ｋｇ）、优降糖（２．５ｍｇ／ｋｇ）和等体积生理盐水（２ｍｌ／只）灌胃，测定各组正常小鼠的血糖水平及以葡萄糖（２ｇ／ｋｇ）、四氧嘧啶（７０ｍｇ／ｋｇ）、肾上腺素（０．０２ｍｇ／ｋｇ）所致小鼠高血糖模型的血糖水平。结果：剂量为１００和３００ｍｇ／ｋｇ的麦冬多糖灌胃对葡萄糖、四嘧啶