全文获取类型
收费全文 | 9128篇 |
免费 | 398篇 |
国内免费 | 40篇 |
专业分类
耳鼻咽喉 | 107篇 |
儿科学 | 222篇 |
妇产科学 | 88篇 |
基础医学 | 1125篇 |
口腔科学 | 305篇 |
临床医学 | 544篇 |
内科学 | 2212篇 |
皮肤病学 | 147篇 |
神经病学 | 663篇 |
特种医学 | 466篇 |
外科学 | 1697篇 |
综合类 | 32篇 |
预防医学 | 167篇 |
眼科学 | 134篇 |
药学 | 481篇 |
中国医学 | 20篇 |
肿瘤学 | 1156篇 |
出版年
2023年 | 39篇 |
2022年 | 79篇 |
2021年 | 121篇 |
2020年 | 62篇 |
2019年 | 108篇 |
2018年 | 163篇 |
2017年 | 157篇 |
2016年 | 183篇 |
2015年 | 170篇 |
2014年 | 254篇 |
2013年 | 309篇 |
2012年 | 504篇 |
2011年 | 591篇 |
2010年 | 339篇 |
2009年 | 356篇 |
2008年 | 548篇 |
2007年 | 598篇 |
2006年 | 612篇 |
2005年 | 604篇 |
2004年 | 635篇 |
2003年 | 593篇 |
2002年 | 575篇 |
2001年 | 82篇 |
2000年 | 85篇 |
1999年 | 134篇 |
1998年 | 166篇 |
1997年 | 153篇 |
1996年 | 151篇 |
1995年 | 113篇 |
1994年 | 119篇 |
1993年 | 121篇 |
1992年 | 65篇 |
1991年 | 69篇 |
1990年 | 60篇 |
1989年 | 53篇 |
1988年 | 46篇 |
1987年 | 44篇 |
1986年 | 48篇 |
1985年 | 36篇 |
1984年 | 47篇 |
1983年 | 46篇 |
1982年 | 43篇 |
1981年 | 42篇 |
1980年 | 32篇 |
1979年 | 19篇 |
1978年 | 29篇 |
1977年 | 17篇 |
1976年 | 16篇 |
1974年 | 17篇 |
1973年 | 15篇 |
排序方式: 共有9566条查询结果,搜索用时 15 毫秒
91.
Tatsuo Sakamoto Hideo Tsukagoshi Peter J. Barnes K. Fan Chung 《Inflammation research》1993,39(3-4):111-117
We have investigated the effects of SR-48968, an NK2 receptor antagonist, and indomethacin, a cyclooxygenase inhibitor, against bronchoconstriction and airway microvascular leakage induced by bradykinin (BK) in anesthetized guinea pigs. In addition, we have determined whether these effects were mediated via bradykinin B2 receptor activation, using a B2 receptor antagonist HOE 140. Lung resistance (R
L) and extravasation of Evans blue dye into airway tissues were used as indexes of airway caliber and microvascular leakage, respectively. BK (15 nmol/kg i.v.) induced a significant increase inR
L and leakage of dye at all airway levels, responses which were completely abolished by HOE 140 (0.13 mg/kg i.v.). SR-48968 (1.5 mg/kg i.v.) had no effect against BK-induced airway effects. Indomethacin (5 mg/kg i.v.) completely blocked the increase inR
L and significantly inhibited the leakage of dye in peripheral intrapulmonary airway. In conclusion, bronchoconstriction induced by i.v. BK is mediated by release of cyclooxygenase products but not by stimulation of NK2 receptors, while the airway microvascular leakage only partly involves cyclooxygenase activation. Cyclooxygenase activation may occur following bradykinin B2 receptor stimulation. 相似文献
92.
Teramoto M Kitawaki J Koshiba H Kitaoka Y Obayashi H Hasegawa G Nakamura N Yoshikawa T Matsushita M Maruya E Saji H Ohta M Honjo H 《American journal of reproductive immunology (New York, N.Y. : 1989)》2004,51(5):352-357
PROBLEM: Tumor necrosis factor (TNF)-alpha is a major cytokine involved in inflammatory and immune function. The aim of this study was to investigate whether polymorphisms at positions -1031, -863 and -857 in the TNF gene promoter region (TNFA) and TNF receptor type 2 gene (TNFR2) are responsible in part for genetic susceptibility to endometriosis. METHODS OF STUDY: TNFA and TNFR2 polymorphisms were determined in 123 patients with endometriosis and 165 fertile healthy women by the polymerase chain reaction (PCR) - preferential homoduplex formation assay and PCR-restriction fragment length polymorphism, respectively. RESULTS: The frequency of the TNFA-U01 haplotype was increased significantly in patients with endometriosis compared with controls (P = 0.045, OR = 1.45). The TNFA-U01 haplotype was strongly associated with HLA-B*0702. No difference was found in TNFR2 polymorphism between patients and controls. CONCLUSION: Our results indicated that TNFA promoter polymorphism was associated with susceptibility to endometriosis. However, this association was not independent of HLA-class I polymorphisms. 相似文献
93.
94.
Michimasa Ebato Taizo Nitta Hideo Yagita Kiyoshi Sato Ko Okumura 《European journal of immunology》1994,24(12):2987-2992
The purpose of this study was to assess the V-(D)-J junctional region of the T cell receptor (TCR), the CDR3 region, which is responsible for glioma-specific antigen contact in αβ TCR-mediated recognition. We sequenced the TCR α and β chians of Vα7, and Vβ13.1 cDNA derived from tumor-infiltrating lymphocytes (TIL) of 12 glioma patients and also the corresponding clones from the patients' peripheral blood lymphocytes (PBL). A shared Vβ13.1 DJ sequence of the CDR3 region, NDβN, was demonstrated in 49 of 66 Vβ13.1+ clones (74.2 %) from the glioma TIL, whereas only 4 of 33 clones (12.1 %) were observed in the Vβ13.1+ clones from the PBL (p < 0.001). A common VDJ sequence, FCASS (Vβ13.1)-YRLPWGTSDS (NDβN)-GELFF(Jβ2.2), was observed not only in the gliomas from each patient, but also among all the patients with a preference for Vβ13.1. In contrast, the amino acid sequences of the Vβ13.1+ PBL clones were diverse and random. Next, we sequenced subclones from other Vβ subfamilies randomly selected to compare their VDJ region rearrangements (Vβ3 and Vβ5.1). In contrast to Vβ13.1, the amino acid sequences of these junctional regions were completely different in these subclones. The V-J junctional region of the α chain is dominated by a few clones in some patients, and no shared amino acid sequences were detected in the TCR Vα junctional region. However, in the Nα region of the Vα7-bearing TIL clones, arginine was used in 27 of 44 clones (61.4%) compared to only 3 of 12 clones from the PBL (p < 0.05). These results are consistent with the hypothesis that a clonal expansion/accumulation of glioma lineage-specific T cells occurred in vivo at the tumor site and that these T cells may be recognizing glioma-specific antigens. 相似文献
95.
Tiina Paunio Yoshihide Sunada Sari Kiuru Hideo Makishita Shu-Ichi Ikeda Jean Weissenbach Jorma Palo Leena Peltonen 《Human mutation》1995,6(1):60-65
Familial amyloidosis, Finnish type (FAF) (gelsolin-related amyloidosis) is an autosomal dominant form of systemic amyloidosis characterized by corneal lattice dystrophy and peripheral polyneuropathy. The accumulating protein in FAF consists of fragments of gelsolin, an actin-modulating protein. The gelsolin mutation G654A has been found in both Finnish and Japanese patients. To study the origin of the gelsolin mutation in these patients we performed haplotype analysis in 10 Finnish and 2 Japanese FAF families. Poymorphic DNA markers GSN, D9S103, AFMa061xd9, and AFMa139xb9 revealed a uniform disease haplotype in all the disease-associated chromosomes of the Finnish FAF families, which was different from the one observed in the Japanese families. The present results and the previously detected gelsolin mutation G654T in Czech and Danish FAF patients suggest that nucle otide 654 may represent a mutation hot spot in the gelsolin gene. The DNA markers studied here will be useful in future genealogical analyses of FAF. © 1995 Wiley-Liss, Inc. 相似文献
96.
97.
Seiiohi Sumino Kazuo Nacsashima Tetsuro Shimamine Toshiaki Abe Hideo Tsuneyoshi Satoru Murao 《Pathology international》1983,33(3):629-643
Autopsy findings in a 40-year-old male with heredofamilial amyloidosis and polyneuropathy are reported. He had been suffering from progressive autonomic as well as sensorimotor dysfunctions. Prominent amyloid deposit was found in the kidney, heart, thyroid, and testis, and less in the interstitium and small vessels of almost all organs. The peripheral nerves, some showing prominent hypertrophy, were most severely involved by amyloid deposit in a form of stellate mass, which ultrastructurally consisted of radially arranged amyloid filaments. In the hypertrophied nerves and ganglia, in addition to amyloid, massive accumulation of acid mucopolysaccharide (AMPS) was seen filling up the interstitial space, which was the cause of hypertrophy. Ultrastructurally, AMPS was seen as finely granular substance. An extracted amyloid from the kidney showed 8 nm filament on negative staining and was estimated of having a molecular weight of 14,000. 相似文献
98.
Yu Nakamura Masatoshi Takeda Hideo Suzuki Hideyuki Hattori Kunitoshi Tada Shiro Hariguchi Shigeo Hashimoto Tsuyoshi Nishimura 《Neuroscience letters》1991,130(2):195-198
Formalin-fixed paraffin-embedded hippocampal sections of brains with early-onset and late-onset Alzheimer's disease were studied immunohistochemically with antisera against cathepsin D and cathepsin B. In addition to the staining of neuronal perikarya, some of the senile plaques visualized by Bielshowsky silver staining and some of reactive astrocytes were positively stained with the antisera against cathepsin D and cathepsin B in brains with Alzheimer's disease. Abnormal localization of cathepsin D and cathepsin B immunoreactivity in neuronal perikarya was observed in brains with early-onset Alzheimer's disease. These findings demonstrate that the distribution of lysosomal proteases was altered in brains with Alzheimer's disease, suggesting the primary and/or secondary involvement of the lysosomal proteases in the pathological process of Alzheimer's disease. 相似文献
99.
Immunohistochemical,ultrastructural and biochemical studies of an amylase-producing breast carcinoma
Hideo Inaji Hiroki Koyama Masahiko Higashiyama Shinzaburo Noguchi Hitoshi Yamamoto Osamu Ishikawa Kaoru Omichi Takeshi Iwanaga Akira Wada 《Virchows Archiv : an international journal of pathology》1991,419(1):29-33
Summary We describe a breast cancer with ectopic production of amylase, found in the patient's serum, urine and in the tumour. Clinically, serum amylase levels reflected both the progression of the disease and regression induced by various therapies. Using agarose gel electrophoresis and a wheat protein inhibitor assay, the predominant serum amylase appeared to be identical to pancreatic-type isoenzyme. However, the action mode analysis using a new fluorogenic substrate revealed that the serum contained non-salivary, non-pancreatic amylase. The tumour had microscopic features of invasive ductal carcinoma with some argyrophilic differentiation. The component cells stained positively for amylase, and ultrastructurally numerous secretory granules were seen. 相似文献
100.
Shigeru Amano Hideo Kataoka Fumitada Hazama Minora Nakatake Atsuhiko Maki 《Pathology international》1985,35(4):969-974
Hepatocellular carcinoma (HCC) showing marked elevation of serum alpha fetoprotein (AFP) (maximum; 70942.0 ng/ml at the end stage) and serum carcinoembryonic antigen (CEA)(maximum; 7368.4 ng/ml at the end stage) was surgically resected. In the resected liver, there were two different tumor nodules which were adjacent to each other but clearly separated by a thin connective tissue. One of the nodules was a well differentiated and the other was poorly differentiated HCC. Immunoperoxidase study revealed that both CEA and AFP were localized in the tumor cells of the poorly differentiated HCC. This is the first report which clearly proved CEA synthesis in the cells of HCC. Serial staining showed that there was simultaneous synthesis of CEA and AFP in some of the tumor cells. ACTA PATHOL. JPN. 35: 969–974, 1985. 相似文献