大学生逃课行为和心理控制点的归因研究

目的通过对大学生逃课行为进行调查和归因研究，来探讨大学生逃课的心理和社会原因。方法采用问卷调查和群体访谈研究方法。结果内外控制个人特征与逃课现象存在显著相关，但男女生的内外控制不存在显著差异；性别与逃课次数是相关的。结论男性比女性更容易逃课；高年级存在更严重的逃课现象；学生群体效应也是不能忽视的原因。由此，学校、老师、学生应关注逃课现象，针对其原因，对大学生逃课行为加强教育和引导，尤其是心理控制点方面的人格完善问题。     

肉苁蓉炮制对微量元素含量及对动物体内DNA合成率的影响

对不同方法炮制的肉苁蓉进行了肝脾脱氧核糖核酸合成率以及金属微量元素的测定分析。结果表明,蒸制盐大芸对“阳虚”动物脱氧核糖核酸及微量元素锌、锰、铜、铁的含量均高于其他的传统炮制品。     

诊断超声对人胚胎安全性的研究

目的　探讨诊断级B超对人胚胎发育的安全性。方法　取 12 0名孕 6～ 8周拟行人工流产孕妇 ,随机分成正常对照组 (Ⅰ组 )、辐照 5min(Ⅱ组 )、10min(Ⅲ组 )、2 0min(Ⅳ组 )、30min(Ⅴ组 )于 1h内取绒毛 ,超声辐照 30min(Ⅵ组 )于 7～ 10d后取绒毛。检测绒毛的生物化学变化。结果　诊断剂量超声辐照 2 0min可致人体宫内绒毛发生生物化学变化。丙二醛 (MDA)值随超声剂量增加而升高 (PⅠ -Ⅲ >0 .0 5,PⅠ .Ⅳ <0 .0 1,PⅠ -Ⅴ <0 .0 1) ,而超氧化物歧化酶及谷胱甘肽过氧化物酶活性随超声辐照剂量增加而下降 (PⅠ -Ⅲ >0 .0 5,PⅠ -Ⅳ <0 .0 1,PⅠ -Ⅴ <0 .0 1)。对照组与Ⅵ组比较P >0 .0 5,提示损伤作用是可逆性的。结论　诊断超声辐照时间 2 0min以上时对绒毛可造成潜在危害     

COMBINED INTRAOPERATIVE ILIAC ARTERY STENTS AND FEMORO-POPLITEAL BYPASS FOR MULTILEVEL ATHEROSCLEROTIC OCCLUSIVE DISEASE

Objective. To review our preliminary experience and evaluate our early results of a combined intraopemtive iliac angioplasty and stenting with infrainguinal revascularization in multilevel atherosclerotic occlusive disease. Methods. From July 1999 to April 2000, intraoperative iliac angiOplasty and stenting combined with simultaneous femoro-pepliteal bypass were pedormed on 12 lower extremities of 10 patients suffering from multilevel athemsclemtic occlusive disease. There were 8 men and 2 women, average 72 years. The indicationsf or procedures included disabling claudication in 3 and rest pain in 7 patients. Results. Eleven iliac angioplasty and stem procedures combined with simultaneous 9 femoro-popliteal by-pass and 3 femoro-femoral-popliteal bypass were performed in 12 limbs of 10 patients. Angioplasty and stem placement was technically successful in all patients. One contralateral femoral-popliteal bypass was failure after femoro-femoral-popliteal bypass. There were no additional instances of procedural or postoperative morbidity or mortality. Mean follow-up was 5 months (range 1 -10 months). During the follow-up period, one femoro-infrapoplitealgraft became occluded after 7 months and above-knee amputation was required. The cumulative primary patencyrate of stented iliac arteries, femoro-femoral bypass grafts and femoro-popliteal bypass grafts were 100% (11/11), 100% (3/3) and 90. 9% (10/11) in the follow-up period, respectivdy. The amputation rate was 8. 3%(1/12). Conclusions. Intraoperative iliac artery PTA and stent placement can be safely and effectively pedormed simultaneously with infrainguinal revascularization for multilevel atherosclerotic occlusive disease by skilled vascular surgeon, using a portable C ann fluoroscopy in the operating room. Furthermore, iliac artery PTA and stenting was valuable adjunct to distal bypass either to improve inflow and outflow, or to reduce the extent of traditionalsurgical intervention, and also, any angioplasty and stenting-related complications can be immediately corrected as well.     

金银花水煎剂对绿脓杆菌P29株R质粒体内体外消除作用的实验研究

目的 :探讨金银花对绿脓杆菌 R质粒消除作用。方法 :体外试验　金银花水煎剂亚抑菌浓度作用绿脓杆菌 P2 948h后 ,影印培养 ,计数消除子 ;体内试验　建立肠道去污染小鼠动物模型后 ,经口感染绿脓杆菌 ,同时给金银花水煎剂 ,灌胃给药 ,2 mg/只 ,2 4 h后再次给药 ,剂量同上 ,48h后处死小鼠 ,分离培养细菌 ,计数消除子数。消除子经质粒抽提琼脂糖凝胶电泳鉴定。结果 :金银花水煎剂对绿脓杆菌 P2 9株 R质粒体外消除率为 0 ,体内消除率为 8%。结论 :金银花水煎剂对去污染小鼠感染绿脓杆菌 P2 9株 R质粒具有消除作用 ,消除子主要表现对单一抗生素恢复敏感性 ;体外没有消除 R质粒作用。     

卵巢癌的p53基因治疗实验研究——人野生型p53基因真核细胞表达质粒的构建

目的 :本实验构建了人野生型 p53基因与真核表达载体 pc DNA3的重组体 ,为进行卵巢癌基因治疗研究打下基础。方法 :利用分子生物学基因克隆技术从质粒 p GEX-p53中用双酶切下目的片段 ,挤压法回收与 pc DNA3构成重组体。结果 :成功的构建了人野生型 p53基因真核细胞表达质粒。结论 :带有两个不同的酶切位点的目的片段定向克隆的方法使重组体的构建高效简便 ,挤压法回收目的片段经济有效。     

Effect of tongbiling on the synoviocyte function in adjuvant arthritis rats

Objective: To study the effect of Tongbiling (TBL) on the proliferation of synovial fibroblast and interleukin-1 (IL-1), tumor necrosis factor-α (TNF-α) and prostaglandin E₂(PGE₂) secreted by synoviocytes in adjuvant arthritis (AA) rats.Methods: Synovial fibroblast was derived from culture of tissue piece. The effect of primary synoviocyte culture supernatants on the fibroblast proliferation were assayed and IL-1, TNF-α bioactivity and PGE₂ content of supernatants of cultured synoviocytes were measured.Results: TBL could significantly inhibit the synovial fibroblast proliferation (P < 0.001), and down-regulate IL-1, TNF-α and PGE₂ productions (P < 0. 001); indomethacin could obviously promote the synovial fibroblast proliferation (P < 0.001). It significantly inhibited PGE₂ production, but further up-regulated IL-1 and TNF-α secreted by synoviocytes (P<0.01).Conclusion: The therapeutical effect of TBL on AA might be associated with its down-regulating the secretory function of synoviocyte, then restoring the abnormal proliferation of fibroblast to normal levels. This program was supported by the Nature Science Foundation of Guangdong Province (No. 960550)     

^131I治疗分化型甲状腺癌的副作用的随访研究

为分析和评价＾１３１Ｉ治疗分化型甲状腺癌的副作用,对３４２例分化型甲状腺癌术后接受了＾１３１Ｉ治疗的患者进行了随访研究。服药后急性反应被记录;出院后定期随访血常规,肝肾功能,涎腺功能及有无与＾１３１Ｉ治疗相关的远期副反应。随访时间１～１０年,平均５．４年。结果：放射性甲状腺炎主要发生在吸＾１３１Ｉ率〉３０％的患者;放射性涎腺炎主要发生在吸＾１３１Ｉ率〈３０％的患者;常见的消化道反应：恶心１２．２％     

预防全椎板切除后椎管再狭窄的实验研究

目的：探讨多孔磷酸钙人工椎板预防全椎板切除后椎管再狭窄的效果。方法：以家兔为动物模型,行全椎板切除术后多孔磷酸钙人工椎板置换,大体观察、观片病理观察、ＣＴ扫描和三维结构重建,计算机图像分析定量测定椎管面积和矢状径。结果：多孔磷酸钙人区椎板节段的椎管面积和矢状径比空白节段明显增大（Ｐ〈０．０１）,与正常对照节段无显著性差异,结论：多孔磷酸钙人工椎板能有效地预防椎板再生后椎管狭窄,并认为椎管狭窄与纤维