预知性健康教育对减轻经尿道膀胱肿瘤电切术后膀胱痉挛的效果观察

目的：探讨术前预知性健康教育对减少和预防膀胱电切术后膀胱痉挛的影响。方法：将71例患者按改进健康教育方法前、后分为对照组和实验组。在一般术前准备和健康教育外对实验组患者增加预知性健康知识给予。结果：分析两组患者的病历和护理记录,发现膀胱痉挛发作时频次、疼痛程度和发作时对护理指导的依从性均明显不同,实验组优于对照组。结论：膀胱电切术前给予预知性教育,减轻焦虑恐惧情绪,可避免或减轻膀胱痉挛发作,增加对术后护理的依从性。     

The application of subspace preconditioned LSQR algorithm for solving the electrocardiography inverse problem

Regularization is an effective method for the solution of ill-posed ECG inverse problems, such as computing epicardial potentials from body surface potentials. The aim of this work was to explore more robust regularization-based solutions through the application of subspace preconditioned LSQR (SP-LSQR) to the study of model-based ECG inverse problems. Here, we presented three different subspace splitting methods, i.e., SVD, wavelet transform and cosine transform schemes, to the design of the preconditioners for ill-posed problems, and to evaluate the performance of algorithms using a realistic heart-torso model simulation protocol. The results demonstrated that when compared with the LSQR, LSQR-Tik and Tik-LSQR method, the SP-LSQR produced higher efficiency and reconstructed more accurate epcicardial potential distributions. Amongst the three applied subspace splitting schemes, the SVD-based preconditioner yielded the best convergence rate and outperformed the other two in seeking the inverse solutions. Moreover, when optimized by the genetic algorithms (GA), the performances of SP-LSQR method were enhanced. The results from this investigation suggested that the SP-LSQR was a useful regularization technique for cardiac inverse problems.     

干部病房老年病人幸福度及相关因素的调查研究

[目的]探讨干部病房老年病人幸福度及相关因素.[方法]对干部病房老年病人进行方便取样调查,采用幸福度量表测量老年病人的生活满意度,采用社会支持评定量表评定老年病人的社会支持情况.[结果]干部病房老年病人的幸福度评分较高(32.30分±9.36分),以正性情感为主;干部病房老年病人的总体幸福度与有无配偶、社会支持水平、探望次数、文化程度密切相关;干部病房老年病人的负性情感与有无配偶、经济状况、性格类型有关.[结论]干部病房老年病人幸福度较高,与其社会支持水平、文化程度、经济状况有密切关系.     

A Study of Factors Inducing Root Resorption During Functional Occlusion in Pre-orthodontic Patients

目的：研究未经正畸治疗的前牙有牙根吸收和无牙根吸收的错[牙合]患者的[牙合]及功能[牙合]特征,揭示引起牙根吸收的可能的[牙合]及咬合因素,为正畸治疗治疗前已有牙根吸收的患者提供可能的[牙合]及功能[牙合]参照标准。方法：用曲面断层片作为筛查方法,并用根尖片进一步确诊根尖吸收,对07年7月至08年3月在我正畸科初诊的患者进行调查,从中选择14名前牙根尖吸收为Ⅱ～Ⅲ度的错[牙合]畸形患者作为研究组,用年龄和性别作为控制标准随机选择14名无根尖吸收的错[牙合]畸形患者作为对照组,采用临床检查、模型分析和DenarMarkⅡ[牙合]架分析的方法研究两组的[牙合]和功能[牙合]特征,用spss14.0进行t检验统计分析。结果：两组的[牙合]特征不存在差异,临床检查和[牙合]架分析的结果均显示前牙牙根吸收组在侧方运动中前牙和后牙出现[牙合]干扰的数量高于对照组,其中[牙合]架分析发现牙根吸收患者在侧方运动中前牙出现[牙合]干扰的数量显著高于对照组（P〈0.05）。结论：未经治疗的错[牙合]畸形患者在侧方运动过程中前牙[牙合]干扰是发生前牙牙根吸收者可能原因。     

Decreased expression of Neurensin-2 correlates with poor prognosis in hepatocellular carcinoma

AIM： To investigate the expression of Neurensin-2 （NRSN2） in hepatocellular carcinoma （HCC） and its prognostic values in predicting survival. METHODS： The expression and prognostic significance of NRSN2 in HCC was examined by performing immunohistochemical analysis using a total of 110 HCC clinical tissue samples, and Western blotting analysis to further confirm the result. RESULTS： Decreased NRSN2 expression was shown in 70.9% cases. Loss of NRSN2 expression in HCC was significantly related to tumor size （P = 0.006）. Larger tumor size was related to negative expression of NRSN2. Patients showing negative NRSN2 expression had a significantly shorter overall survival than those with positive expression （P = 0.008）. Multivariate Cox regression analysis indicated that NRSN2 expression level was an independent factor of survival （P = 0.013）. Western blotting analysis further confirmed decreased expression of NRSN2 in tumor tissues compared with non-tumorous tissues. CONCLUSION： Our study indicated that NRSIV2 could be a tumor suppressor gene for HCC and a candidate biomarker for long-term survival in HCC.     

构建职业特色校园文化 培养高职学生职业精神

职业教育的健康发展和蓬勃兴旺首先依赖于职业精神的发扬光大。职业精神是与人们的职业活动紧密联系,具有自身职业特征的精神,它能鲜明地表达职业根本利益以及职业责任、职业行为上的精神要求。其基本要素有职业理想、职业态度、职业责任、职业技能、职业精神、职业良心、职业信誉和职业作风等。对于高职学生职业精神的培养,     

谷氨酸脱羧酶抗体在临床初诊2型糖尿病病程中的演变

目的观察谷氨酸脱羧酶抗体(GADAb)在临床初诊2型糖尿病(T2DM)病程中的变化情况。方法在我院内分泌科连续入组病程1年以内的初诊T2DM患者共303例。每半年或1年复查GADAb,每年复查空腹C肽,每3个月检测糖化血红蛋白。随访1~6(中位数1·2)年。结果入组时GADAb阴性的265例患者随访2年内保持阴性不变;38例GADAb阳性,随访结束时16例转阴;5例表现为抗体阳性→阴性→阳性波动;17例始终保持抗体阳性。GADAb整体上随病程发展其滴度减低(P<0·05),且其变化仅与初始抗体滴度呈负相关(P<0·01)。结论GADAb在临床初诊T2DM病程中存在变化。单次检测GADAb有可能漏诊自身免疫性糖尿病。     

甲醛染毒对雄性大鼠脏器及血清微量元素的影响

目的探讨甲醛染毒对雄性大鼠脏器及血清微量元素的影响.方法选用体重(110～130)g Wistar大鼠40只,随机分为4组, 染毒浓度分别为,高浓度组:(119.6±15.4) mg/m3、中浓度组:(25.2±4.6) mg/m3、低浓度组:(4.8±1.3) mg/m3和对照组;室内空气,采用静式吸入法,每天染毒1h,连续染毒9w后处死,测定大鼠各脏器的脏器系数、甲醛浓度、及大鼠血清中Fe,Cu,Zn的含量.结果高剂量组雄性大鼠肺、肾和肝脏中甲醛浓度明显高于对照组,各染毒组雄性大鼠体重明显低于对照组(P<0.05),高剂量组雄性大鼠各脏器的脏器系数与对照组比较均有显著性差异(P<0.05),雄性大鼠血清中锌含量明显降低、铜含量显著升高(P<0.05).结论长期高剂量吸入甲醛可影响雄性大鼠的生长及其血清Cu、Zn水平,并可损伤雄性大鼠的肝、肺、肾、心、脑、睾丸.     

老年妇科手术围手术期临床特点分析

目的探索老年妇科手术围手术期的临床特点。方法2001年1月至2004年3月老年妇女(年龄≥60岁)妇科手术病人50例为研究组,同期中年妇女(40～59岁)50例为对照组。监测麻醉前、达到完全阻滞平面时以及手术结束时病人的收缩压(SBP)、舒张压(DBP)、平均动脉压(MAP)、心率(HR)、血氧饱和度(SPO2)值,及术后最高体温、发热持续时间和其它并发症情况。结果两组病人达到完全阻滞平面时、手术结束时的SBP、DBP、MAP均较麻醉前显著降低(P<0.05),老年组下降幅度更大(P<0.001),而HR、SPO2及术后发热情况无明显差异。老年组术后并发症率16%,其中一例死于急性肺栓塞。中年组术后并发症率10%。结论老年妇女对妇科手术的耐受性较好,加强围手术期监护有助于提高手术的安全性。     

Blockade of Rho/Rho-associated coiled coil-forming kinase signaling can prevent progression of hepatocellular carcinoma in matrix metalloproteinase-dependent manner

Aim:  There is growing evidence that the Rho/Rho-associated coiled coil-forming kinase (ROCK) signaling pathway is upregulated in tumors and plays a key role in cancer invasion and metastasis. Our aim was to test the anticancer effects of Rho/ROCK inhibitor, Y-27632, including possible mechanisms in a highly-metastasizing hepatocellular carcinoma (HCC) mouse model on its secretion of matrix metalloproteinase (MMP) and tumor progression.
Methods:  Following orthotopic implantation of CBO140C12 HCC tumor fragments into the liver of mice, the mice were randomly assigned to a Y-27632-treated group or control group. After treatment for 4 weeks, specimens were obtained to evaluate tumor size, metastases, and immunohistochemical findings. In vitro , we examined the effects of Y-27632 and RhoC siRNA on MMP-2 and -9 expressions, invasiveness, and apoptosis in cultured tumor cells.
Results:  Both RhoA and RhoC were upregulated in HCC-bearing livers, and Y-27632 significantly inhibited not only tumor growth and intrahepatic metastasis ( P  < 0.05), but also tumoral MMP-9 expression. Moreover, Y-27632 treatment resulted in large necrotic areas in tumors. In vitro , Y-27632 and RhoC siRNA reduced MMP-2 and -9 expressions, as well as the chemotactic migration of tumor cells dose-dependently, and increased apoptosis eight times.
Conclusion:  Y-27632 suppresses progression and limits the intrahepatic metastasis of established HCC. This could be linked to the decreased MMP expression and induction of apoptosis in tumor cells. Rho signaling may prove to be a productive target in anticancer therapy.