脾动脉栓塞治疗外伤性脾破裂28例临床疗效观察

目的：探讨脾动脉栓塞术对外伤性脾破裂的疗效及并发症的处理。方法：采用Seldinger法行脾动脉栓塞术治疗外伤性脾破裂28例。结果：所有患者出血立即停止，其中4例行2次栓塞。28例均有脾区疼痛，22例发热。脾动脉栓塞5天后血小板、白细胞上升近1倍，7天后恢复至正常范围。28例随访6－36月，查免疫球蛋白、CT等结果满意，未有再出血及发生暴发性感染。结论：脾动脉栓塞不但有良好的止血作用，而且能保留脾脏的免疫功能，可在非手术治疗失败时选用，是外伤性脾破裂的一种有效治疗方法。     

干扰素病毒唑联合治疗慢性丙型肝炎疗效及干扰素抗体的影响

目的 :研究干扰素与病毒唑联合治疗慢性丙型肝炎疗效及干扰素抗体的影响。方法 :观察组 2 0例采用干扰素 α2b 3MvTiwIH +病毒唑 10 0 0mgQdpo连用 2 4周 ;对照组 3 2例采用干扰素 α2b 3MvTiwiH连用 2 4周。停药后观察 2 4周比较疗效并检测干扰抗体。结果 :观察组完全应答率为 5 0 % ,明显高于对照组 18.7% ;复发率为 2 5 .0 %低于对照组 5 6.3 % (P <0 .0 1)。无反应率均为 2 5 .0 %。且复发与干扰素抗体尤其是中和抗体有关。结论 :观察组疗效优于对照组 ,复发与干扰素抗体尤其是中和抗体有关。     

Altered swelling behavior of femoral cartilage following joint immobilization in a canine model.

Periods of reduced joint loading have been shown to induce changes in the biochemical composition. metabolism and mechanics of articular cartilage. In this study, changes in cartilage swelling behavior were studied following a 4-week period of joint immobilization, using a recently developed osmotic loading technique [J. Biomech, 32 (1999) 401-408]. The magnitude and distribution of swelling strains were measured in cartilage-bone samples equilibrated in physiological and hypotonic saline, relative to a hypertonic reference NaCl solution. Physicochemical parameters (glycosaminoglycan fixed charge density and water volume fraction) were determined in site-matched cartilage samples. The experimental data for swelling strains, fixed charge density and water volume fraction were used with a triphasic mechano-chemical theory [J. Biomech. Eng. 113 (1991) 245-258] to determine the effect of joint immobilization on the tensile modulus of the cartilage solid matrix. Four weeks of immobilization resulted in a significant increase in the magnitude of swelling-induced strains, and a significant decrease in fixed charge density in cartilage, as compared with the contralateral controls. Joint immobilization also resulted in decreases in values for the modulus of cartilage, as compared with the contralateral controls. Our results suggest that 4 weeks of joint immobilization had a significant effect on cartilage mechanical function that may be linked to collagen changes in the cartilage extracellular matrix.     

The Effect of Acupuncture on Bone Mineral Density in Postmenopausal Women

According to the TCM theory that the kidney is in charge of the bone, the authors carried out a study on the effect of acupuncture on bone mineral density in 40 postmenopausal women with osteoporosis treated by the method of reinforcing the kidney to strengthen the bone, with satisfactory therapeutic results reported as follows.     

寒冷干燥地区平时火器伤的早期处理

目的　探讨寒冷干燥地区平时火器伤伤情特点与早期救治方法。方法　对 1991年 1月— 2 0 0 2年 12月间 40例火器伤病人的临床救治进行回顾分析。结果　 2 4例枪伤中 ,2 0例一期缝合伤口 ,均一期愈合 ,另外 4例只清创未一期缝合的伤口 ,3例愈合 ,1例感染 ;4例骨折内固定均愈合良好 ,无骨髓炎发生。 16例炸伤病人局部软组织损伤及污染比枪弹伤重 ,14例一期缝合伤口中 ,10例一期愈合 ,4例感染 ,骨折内固定 2例 ,均愈合良好 ,无骨髓炎发生。结论　寒冷干燥地区火器伤早期伤道清创时限可适当延长 ,在平时火器伤的救治中 ,对软组织条件较好的创面 ,早期清创后可一期闭合伤口和骨折内固定 ,这样可减少感染和伤残 ,促进康复     

Gender-specific response to isoflurane preconditioning in focal cerebral ischemia.

Inhalation anesthetics are effective chemical preconditioning agents in experimental cerebral ischemia. However, previous work has been performed exclusively in male animals. We determined if there is a gender difference in ischemic outcome after isoflurane preconditioning (IsoPC), and if this sex-specific response is linked to differences in Akt phosphorylation or expression of neuronal inducible cell-death putative kinase (NIPK), a negative modulator of Akt activation. Young and middle-aged male and female mice were preconditioned for 4 h with air (sham PC) or 1.0% IsoPC and recovered for 24 h. Cortices were subdissected from preconditioned young male and female mice for measurement of Akt phosphorylation (Western blot) and NIPK mRNA (quantitative polymerase chain reaction). Additional cohorts underwent 2 h of reversible middle cerebral artery occlusion. Lastly, male and female Akt1(+/+) and Akt1(-/-) mice were studied to determine if gender differences in ischemic outcome after IsoPC is Akt1-dependent. Infarction volume was determined at 22 h reperfusion (2,3,5-triphenyltetrazolium chloride). As expected, IsoPC decreased ischemic damage as compared with sham PC in young and middle-aged male mice. In contrast, IsoPC markedly increased infarction in young female mice and had no effect in middle-aged female mice. Cortical phospho-Akt was increased by IsoPC versus sham PC only in male mice. No increase was observed in IsoPC female mice. NIPK mRNA was higher in female mice than in male mice regardless of preconditioning status. Male IsoPC neuroprotection was lost in Akt1-deficient male mice. We conclude that IsoPC is beneficial only in ischemic male brain and that sex differences in IsoPC are mediated through Akt activation and basal NIPK expression.     

风痛灵颗粒制备工艺研究

目的：为了优化风痛灵颗粒的制各工艺。方法：采用正交试验以阿魏酸、多糖等为指标。结果：得出最佳的浸提工艺参数，同时考察了最佳的喷雾干燥及制粒条件。结论：本方法是风痛灵颗粒的最佳的制备方法。     

大肠癌Lovo细胞E-Cadherin,N-Cadherin的表达及化学治疗药物对其表达的影响

目的：研究大肠癌Lovo细胞上皮钙粘附素(E-Cadherin)，神经钙粘附素(N-Cadherin)的表达及常用化学治疗药物对其表达的影响。方法：应用逆转录聚合酶链反应(RT-PCR)法检测大肠癌Lovo细胞E-Cad-herin，N-Cadherin的表达，并通过几种不同的化疗药物(顺铂、吡柔比星、丝裂霉素、5-FU)不同浓度和时间作用后，对E-Cadherin，N-Cadherin表达的影响。结果：不同化疗药物对大肠癌Lovo细胞N=Cadherin表达无影响，高浓度顺铂和高浓度吡柔比星二组出现-Cadherin表达，其它各组未见表达。结论：大肠癌常用的化疗药物无论低浓度持续用药，还是高浓度短时间用药，对大肠癌Lovo细胞N-Cadherin的表达无抑制作用，说明上述药物不能通过抑制N—Cadherin的表达，而起到抗癌作用。高浓度顺铂和吡柔比星二组显示E-Cadherin的表达，表现出抑癌的作用，从而提示在大肠癌化疗时，顺铂和吡柔比星更适合于高浓度短时间应用。     

抗中性粒细胞胞浆抗体在肾小球疾病中的检测及临床意义

目的:检测各种肾小球疾病患者血清中抗中性粒细胞胞浆抗体(ANCA)并探讨其临床意义。方法:应用间接免疫荧光法(IIF)检测20例正常人及131例各种肾小球疾病患者血清中ANCA。结果:131例患者中有11例ANCA阳性性(阳性率为8.4％),且均为P-ANCA阳性,而20例正常对照组无1例阳性(P<0.05)。ANCA阳性中以新月体性肾炎、狼疮性肾炎、紫癜性肾炎的阳性率较高,分别为67％、25％、16.7％。新月体性肾炎与IgA肾病等原发性肾小球疾病组比较有显著性差异(P<0.05)。结论:ANCA在各种肾小球疾病中并不少见,尤其是新月体性肾炎、狼疮性肾炎、紫癜性肾炎的阳性率较高,推测ANCA可能在这些疾病的发病机理中起了一定的作用。