产妇自控硬膜外阻滞分娩镇痛的临床研究

为观察产妇自控硬膜外阻滞在分娩镇痛中应用的可行性,选择ＡＳＡⅠ－Ⅱ级,估计能经阴道分娩并志愿行为分娩镇痛的足月初产妇４４例,采用产妇自控硬膜外阻滞对其进行分娩镇痛;以同期未行分娩镇痛而其它条件相当的足月初产妇４６例为对照。     

Bcl—2和nm23蛋白在上皮性卵巢中的表达及其临床意义

采用免疫组织化学技术,对４５例上皮性卵巢癌组织中ｂｃｌ－２、ｎｍ２３蛋白的表达状况进行研究。结果示,ＢＣＬ－２蛋白的阳性表达率为６０％,其表达与组织学分级有关,ｎｍ２３蛋白的阳性表达率为４０％,其表达与临床分期有关。提示,对这两个指标的检测有利行估计卵巢癌病人的预后。     

人芽囊原虫滋养体和包囊的形态学观察

用普通光学显微镜和荧光显微镜对人芽囊原虫的三株滋养体和二株包囊进行了形状、大小和结构的观察。滋养体为生长在ＬＡＳ双相培养基内４～５天的培养物;包囊为带虫者粪便经蒸馏水反复３次洗涤的沉淀物,均作涂片,分别以不染色、碘染色和１：１０＾４吖啶橙染色,镜下识别和鉴定,滋养体以空泡型（包括二分裂状态的）占多数,其次为变形型,颗粒型及其它类型少见,颗粒型为多分裂繁殖体,人芽囊原虫包囊形状较单一,都是圆形或卵圆     

社会文化与知情同意的伦理原则

由于社会文化的原因,知情同意原则在我国并未得到充分的认同,但对知情同意原则未实施的原因应具体分析,对癌症病人的心理能力的考虑,病人知情同意权利意识的缺乏,医务人员对病人知情权利的漠视,缺乏知情同意实施的社会文化土壤是知情同意原则在我国衽的主要阻力。     

手术治疗甲状腺疾病的临床研究（附2092例报告）

目的：探讨甲状腺疾病的外科治疗。方法：回顾性研究近２０年来甲状腺疾病２０９２例。结果：３手术治愈率１００％,死亡率为零,术后并发症甲亢４．４％,甲状腺癌３．３％,甲状腺瘤０．４４％。结论：手术治疗甲头腺疾病是安全、有效、合理可行的。术前充分准备是减少并发症的关键。     

P^27的研究概况

目的：综述Ｐ＾２７的研究进展。方法：光盘检索有关献,归纳综合整理。结果：Ｐ＾２７是细胞周期的相关蛋白,Ｐ＾２７白过度表达可抑制细胞周期的进程,阻断细胞生长于Ｇ１期。结论：Ｐ＾２７是细胞周期中重要的负性调控控因子,是一种新的侯选的肿瘤抑制基因。     

Characterization of lipopeptide produced by Bacillus altitudinis Q7 and inhibitory effect on Alternaria alternata

This study identified the antifungal metabolites produced by Bacillus altitudinis Q7 against Alternaria alternata and investigated the antifungal activity and antifungal action. Lipopeptide, the important secondary metabolites were identified by Fourier transform infrared (FTIR) and liquid chromatography-mass spectrometry as lichenysin. The antifungal activity of lipopeptide on A. alternata was determined by microdilution technique, and its minimum inhibitory concentration was 1.2 mg/ml. Stability test showed that lipopeptide had excellent temperature and pH resistance. To investigate whether lichenysin acted on the cell membrane and changed its permeability, the ultra-violet absorption of protein and nucleic acid were measured using a colorimetric method. The antifungal metabolites produced by B. altitudinis Q7 was lichenysin, which showed stable antifungal activity in the extreme environments. Lichenysin could inhibit A. alternata by altering the permeability of cell membrane, leading to the outflow of proteins and nucleic acids from the cytoplasm. This research suggests the lipopeptide from B. altitudinis Q7 is a potential biological control agent against A. alternata.     

Simultaneous Construct Surface Microstructural and Internal Network-Like Conductive Pathways of Poly(l-lactic acid)/Carbon Nanomaterials Composite Foams

The poly(l -lactic acid) (PLLA)/carbon nanomaterials composite foams with hierarchical surface microstructural and internal conductive pathways are successfully prepared by a simple crystallization-assisted rapid phase separation (CARPS). The dimension and morphology of carbon nanomaterials can induce different crystallization forms to construct the hierarchical surface microstructure, and they are distributed on the phase interface of solvent and non-solvent to form conductive pathways. It is found that the heterogeneous nucleation of nanomaterials promotes a significant increase in crystallinity, and a stacked granular structure formed on the surface promotes the increase of the water contact angle to 148.7°. Foams with interconnecting pore structures contribute to the formation of 85.3% porosity and 12.33 g g⁻¹ oil absorption. Carbon nanomaterials are distributed on the pore walls of the porous foam, which converts the foam from an insulating material to a conducting polymer. Furthermore, the uniform distribution of nanomaterials significantly affects the thermal stability of the PLLA. In belief, the multifunctional biodegradable foam, prepared by a CARPS method, makes it promising for industrial production and has potential applications in electrical conductivity, oil-water separation, and many other fields.     

DNMT3A对骨关节炎软骨细胞SOX9基因DNA甲基化修饰的影响

目的 探讨DNMT3A对SOX9基因DNA甲基化修饰的影响。方法 培养正常软骨细胞和OA软骨细胞,显微镜下观察软骨细胞形态,甲苯胺蓝染色、Ⅱ型胶原染色鉴定软骨细胞,qRT-PCR、Western Blot检测DNMT3A、SOX9 mRNA和蛋白表达水平,亚硫酸氢盐测序检测SOX9基因DNA甲基化状态,慢病毒沉默DNMT3A观察SOX9基因DNA甲基化修饰的变化。结果 正常软骨细胞形态规则,体积较大,数量较多,OA软骨细胞形态不规则,体积较小,数量较少。与正常软骨细胞比较,OA软骨细胞中DNMT3A mRNA和蛋白表达量增高（P＜0.05）,SOX9 mRNA和蛋白表达量明显降低（P＜0.05）,SOX9基因DNA甲基化百分比增高。在OA软骨细胞中沉默DNMT3A后,SOX9基因DNA甲基化百分比明显降低。结论 DNMT3A调节SOX9基因DNA甲基化修饰参与OA软骨细胞病变。     

人原发性肝细胞癌核基质蛋白的研究

目的比较正常肝与原发性肝细胞癌（ＨＣＣ）核基质蛋白的异同,观察是否有ＨＣＣ特异性核基质蛋白的存在。方法用双向电泳方法比较了３例正常肝和８例ＨＣＣ核基质蛋白成分。结果正常肝和ＨＣＣ的核基质蛋白组成极为相似,但在ＨＣＣ中发现至少有４个ＨＣＣ的特异性核基质蛋白。其中以分子量为６２０００、等电点为５．３的蛋白最具代表性,它存在于所研究的８例ＨＣＣ中。其余３个ＨＣＣ特异性蛋白亦存在于大多数ＨＣＣ中。３例正常肝组织中未见有这４个ＨＣＣ特异性核基质蛋白。结论ＨＣＣ确实有ＨＣＣ特异性核基质蛋白的存在,它的发现可能会为ＨＣＣ的发生、发展、发病机理的研究提供一个新途径。