丙氧鸟苷对转TK基因的人外周血单个核细胞的毒性作用

目的 探讨减少异体干细胞移植中移植抗宿主病（GVHD）,同时最大限度提高移植抗白血病（GVL）效应的有效方法。方法 采用逆转录病毒介导的基因转移方法将I型单纯疱疹病毒胸苷激酶（HSV－TK）基因、绿色荧光蛋白（GFP）基因及抗新霉素（NeoR）基因插入人外周血单个核细胞（PBMC）,MTT法测定丙氧鸟苷（GCV）对转化细胞抑制率。结果 转化细胞表达GFP,且主要为T淋巴细胞,占11．4％,而且被转化的T淋巴细胞中,CD4阳性细胞转化率较高,占7．6％,CD8,CD19,CD33阳性细胞转化率分别为2．9％,2．1％和4．7％,PCR鉴定表明,转染的人外周血单个核细胞DNA中整合中有NeoR基因,MTT法测定丙氧鸟苷（GCV）对转化细胞与未转化细胞抑制率,显示转化细胞生长明显受抑。结论 异体干细胞移植后,如产生严重GVHD应用GCV选择性清除HSV－TK基因转导的T淋巴细胞,使控制GVHD已成可能。     

Effect of Transforming Growth Factor-β_2 on Phagocytosis in Cultured Bovine Trabecular Meshwork Cells

A gradual loss of cells occurs within the humantrabecular meshwork (TM) during normal aging andappears to be increased in patients with primary openangle glaucoma (POAG) [1] .The exactmechanism bywhich cells are lost in either condition is not known,however phagocytosis has been suggested[2 ] .It hasbeen found that,when compared with the non- POAGpatients,the level of transforming growth factor- β2(TGF- β2 ) which may be involved in the pathogenesisof POAG increased in the aqueous humo…     

Irritable bowel syndrome: A technical review for practice guideline development

This article has no abstract. To view the article, select the "View Print Version (PDF)" link above.     

Epidemiology, values, and the meaning of paradigm

Use of the term paradigm has recently reached the epidemiological milieu. This article provides a critical analysis of a classification of "epidemiological paradigms" as proposed by Almeida-Filho. It was initially ascertained that values were not considered fundamental elements for the construction of paradigms, and that they therefore distanced themselves from the Kuhnian matrix. Systematization sought to unite antithetical tendencies and thus seemed closer to the Foucaltian epistéme. Finally, hegemony was considered a more appropriate term than paradigm for systematizing epidemiological periods, since the values of the different watersheds are committed to particularity (principally social class).     

左旋卡尼汀治疗不稳定型心绞痛疗效观察

目的:探讨左旋卡尼汀治疗不稳定性心绞痛的临床疗效.方法:86例不稳定性心绞痛患者随机分为两组,每组43例.在常规治疗基础上,治疗组应用左旋卡尼汀,对照组应用极化液.结果:心绞痛缓解有效率治疗组为93.02%,对照组心绞痛缓解有效率为65.12%(P＜0.01);静息心电图缺血性ST段恢复有效率治疗组为76.74%,对照组为48.84%(P＜0.01).结论:左旋卡尼汀辅助治疗不稳定性心绞痛的是一种安全有效的方法,它能改善缺血心肌的能量代谢.     

Monocyte-dependent regulation of T lymphocyte activation through CD98

CD98 is a 125 kDa heterodimer, which is strongly expressed on the surface of activated and proliferating cells. Its expression is strikingly regulated during T cell differentiation and activation, but the role of CD98 during T lymphocyte responses is not yet understood. We report here that proliferation of resting peripheral blood mononuclear cells (PBMC) induced by lectin, superantigen (SAg) or conventional antigens was blocked by anti-CD98 heavy chain (CD98hc) mAb. In contrast, anti-CD98hc did not block responses of T cell clones or lines. Anti-CD98hc inhibited IL-2 receptor expression and progression of T cells from G1 to S phase, but did not reduce expression of the IL-2 gene. Anti-CD98hc mAb did not regulate the initial activation events involving the TCR and co-receptor structures, but instead inhibited T lymphocyte responses even when added 18 h or more after the activation stimulus. Further experiments demonstrated that anti-CD98 was not directly affecting T cells in this system, but was instead acting on accessory cells. This was supported using a novel xenogeneic system that takes advantage of the lack of xenoreactivity of purified human T cells against mouse splenocytes. Despite absence of a direct xenoresponse to murine spleen cells, human T cells were activated by SAg presented by murine splenic antigen-presenting cells (APC). Murine anti-human CD98hc did not block T cell proliferation in this system. Furthermore, responses using monocyte-depleted PBMC as APC were not blocked by anti-CD98hc. Taken together, the present data suggests that triggering of human monocyte CD98 can suppress T cell proliferation by a process that halts progression through the cell cycle of recently activated T lymphocytes. This may represent a novel pathway for monocyte regulation of T cell activation.      

南京市7岁以下儿童上臂围及上臂围与身高比值调查分析

目的 对2015年南京市7岁以下儿童上臂围及上臂围/身高比值 (AHtR) 进行调查分析,探讨上臂围及上臂围/身高比值在儿童生长发育监测中的意义。方法 采用分层随机整群抽样方法,分男、女各21个年龄组,共测量8 682名7岁以下儿童的上臂围,与体格相关指标进行分析。结果 上臂围在1~3月龄间增长速度较快,3月龄后增长逐渐缓慢;不同年龄组男童上臂围平均值均大于女童组,其中1月~、3~21月、2~<2.5岁、3~<4.5岁及5.5~<7岁组上臂围在性别上差异有统计学意义(P＜0.05);前3个月,AHtR呈现随月龄增加上升趋势,此后随月龄增加逐渐下降。1月~<3岁各年龄组AHtR平均值范围为男童0.169~0.237,女童0.169~0.230;3~<7岁各年龄组AHtR中位数范围为男童0.150~0.160;女童0.140~0.160。上臂围均与体重、身长/高存在正相关性,与体重指数 (BMI)无相关性。结论 南京市7岁以下儿童上臂围存在年龄和性别上的差异,AHtR存在年龄差异,上臂围与其体重相关。     

胃肠癌患者术后生命质量现状及与焦虑、抑郁的相关性分析

目的 探讨胃肠癌患者生命质量现状及与焦虑、抑郁的相关性,为提高患者生命质量提供依据。 方法 采用医院焦虑抑郁量表(Hospital Anxiety and Depression Scale, HADS)和癌症患者生命质量测定量表(Functional Assessment of Cancer Therapy Generic Scale, FACT-G)评估198例胃肠癌术后住院患者焦虑、抑郁情绪障碍和生命质量情况,采用方差分析或t检验分析不同特征及焦虑、抑郁程度胃肠癌患者生命质量情况,并采用Pearson相关分析胃肠癌患者生命质量与焦虑、抑郁情绪障碍程度的相关性。 结果 198例研究对象均为胃肠癌术后患者,其中胃癌、结肠癌、直肠癌分别为84例、51例、63例。198例研究对象FACT-G总评分为(59.62±7.94)分,不同年龄段、不同肿瘤位置、不同术前TNM分期、不同确诊时间和不同化疗次数,FACT-G总评分差异有统计学意义(P<0.001)。其中,轻度、中度、重度焦虑患者分别为79例(39.90%)、66例(33.33%)、22例(11.11%)。轻度、中度、重度抑郁患者分别为82例(41.41%)、42例(21.21%)、16例(8.08%)。不同焦虑、抑郁程度患者间FACT-G总评分以及各维度评分差异均有统计学意义(P<0.05)。Pearson相关分析结果显示胃肠癌患者生命质量总分与抑郁、焦虑均呈较强相关,Pearson相关系数分别为-0.43和-0.46;生命质量各维度评分与焦虑、抑郁评分均呈显著负相关。 结论 胃肠癌术后患者年龄越大、术前TNM分期越高、确诊时间越长、放化疗次数越多患者术后整体生命质量越低;焦虑和抑郁发病率较高,术后生命质量总分及各维度评分均与焦虑、抑郁评分呈显著负相关。