全文获取类型
收费全文 | 5556篇 |
免费 | 422篇 |
国内免费 | 63篇 |
专业分类
耳鼻咽喉 | 57篇 |
儿科学 | 156篇 |
妇产科学 | 223篇 |
基础医学 | 716篇 |
口腔科学 | 75篇 |
临床医学 | 449篇 |
内科学 | 1415篇 |
皮肤病学 | 143篇 |
神经病学 | 724篇 |
特种医学 | 178篇 |
外科学 | 500篇 |
综合类 | 7篇 |
一般理论 | 3篇 |
预防医学 | 348篇 |
眼科学 | 60篇 |
药学 | 360篇 |
中国医学 | 9篇 |
肿瘤学 | 618篇 |
出版年
2024年 | 14篇 |
2023年 | 136篇 |
2022年 | 245篇 |
2021年 | 445篇 |
2020年 | 257篇 |
2019年 | 288篇 |
2018年 | 340篇 |
2017年 | 211篇 |
2016年 | 237篇 |
2015年 | 264篇 |
2014年 | 275篇 |
2013年 | 323篇 |
2012年 | 446篇 |
2011年 | 372篇 |
2010年 | 205篇 |
2009年 | 157篇 |
2008年 | 263篇 |
2007年 | 273篇 |
2006年 | 242篇 |
2005年 | 209篇 |
2004年 | 174篇 |
2003年 | 165篇 |
2002年 | 124篇 |
2001年 | 38篇 |
2000年 | 26篇 |
1999年 | 33篇 |
1998年 | 33篇 |
1997年 | 21篇 |
1996年 | 18篇 |
1995年 | 14篇 |
1994年 | 15篇 |
1993年 | 18篇 |
1992年 | 11篇 |
1991年 | 13篇 |
1990年 | 17篇 |
1989年 | 20篇 |
1988年 | 20篇 |
1987年 | 16篇 |
1986年 | 10篇 |
1985年 | 10篇 |
1984年 | 5篇 |
1983年 | 4篇 |
1982年 | 6篇 |
1981年 | 5篇 |
1979年 | 7篇 |
1978年 | 4篇 |
1977年 | 3篇 |
1976年 | 2篇 |
1973年 | 2篇 |
1966年 | 1篇 |
排序方式: 共有6041条查询结果,搜索用时 15 毫秒
11.
Candida albicans yeast and germ tube forms interfere differently with human monocyte differentiation into dendritic cells: a novel dimorphism-dependent mechanism to escape the host's immune response
下载免费PDF全文
![点击此处可从《Infection and immunity》网站下载免费的PDF全文](/ch/ext_images/free.gif)
12.
di Gioia CR Ciallella C d'Amati G Parroni E Nardone AM Gallo P 《Archives of pathology & laboratory medicine》2003,127(9):e367-e370
An infant with normal facies and none of the extracardiac anomalies usually associated with Williams syndrome presented at birth with an echocardiographic pattern of supravalvular pulmonary stenosis and displastic pulmonary valve. A clinical reappraisal was planned at 3 months of age, but the girl died suddenly at home at 2 months of age. At autopsy, both ventricles were hypertrophic, and the valves showed mild dysplasia. The walls of the great arteries were thick, with a "washed leather" consistency, but there was no gross evidence of discrete stenosis. The histologic mosaic appearance of the media of the great arteries, due to elastosis and extreme disarray of the elastic lamellae, prompted a postmortem diagnosis of supravalvar aortic stenosis and suggested a diagnosis of Williams syndrome, which was subsequently confirmed by fluorescence in situ hybridization. Pediatricians and pathologists should be alerted that Williams syndrome in the newborn may present as an isolated supravalvular pulmonary stenosis, whereas supravalvular aortic stenosis becomes clinically significant only a few months later. 相似文献
13.
d'Amati G Bagattin A Bauce B Rampazzo A Autore C Basso C King K Romeo MD Gallo P Thiene G Danieli GA Nava A 《Human pathology》2005,36(7):761-767
We report on a family with a history of sudden death and effort-induced polymorphic ventricular arrhythmias. The index case was a 17-year-old boy who died suddenly and at postmortem had evidence of fibrofatty replacement in the right ventricular free wall, consistent with arrhythmogenic right ventricular cardiomyopathy, as well as calcium phosphate deposits within the myocytes. A molecular genetics investigation carried out in the paraffin-embedded myocardium of the subject and in blood samples of family members disclosed a missense mutation in exon 3 (230C-->T; A77V) of the cardiac ryanodine receptor type 2 gene. The carriers showed effort-induced polymorphic ventricular tachycardia in the setting of normal resting electrocardiogram and trivial echocardiographic abnormalities, consistent with catecholaminergic polymorphic ventricular tachycardia. The observation of both arrhythmogenic right ventricular cardiomyopathy type 2 and catecholaminergic polymorphic ventricular tachycardia in the same family suggests that the two entities might correspond to different degrees of phenotypic expression of the same disease. This experience underscores the importance of a precise autopsy diagnosis in the case of sudden cardiac death, including molecular genetics, and the mission of pathologists to guide further clinical investigation of family members. 相似文献
14.
Differential Expression of Matrix-Metalloproteinase-1 and -2 Genes in Normal and Fibrotic Human Liver 总被引:17,自引:9,他引:17
下载免费PDF全文
![点击此处可从《The American journal of pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
15.
Giulia Kramar Teresa Aguado Jacques Louis Giuseppe Del Giudice Paul-Henri Lambert 《European journal of immunology》1993,23(10):2551-2556
BALB/c mice injected at birth with semi-allogeneic F1 spleen cells become tolerant to alloantigens as shown by their CTL unresponsiveness to the corresponding alloantigen and the persistence of donor F1 cells into the BALB/c host. Moreover, these mice develop a transient systemic lupus erythematosis-like autoimmune syndrome characterized by splenomegaly, glomerulonephritis, thrombocytopenia and abnormal serological findings, such as several autoantibodies and IgG1 hypergammaglobulinemia. Recent studies done in our laboratory have shown that donor F1 B cells persisting in the host are responsible for the production of autoantibodies and must be activated in vivo by the host CD4+ T lymphocytes in a MHC class II-restricted fashion. In the present work, we have focused our attention on the ability of splenic CD4+ T cells recovered at different periods from BALB/c mice injected at birth with (CBA/Ca × BALB. Ighb) Fl spleen cells to interact with and activate F1 semi-allogeneic spleen cells in vitro. We show that (i) only CD4+ T cells from 2- and 3-week-old tolerant BALB/c mice preferentially produce IL-4 and IL-5 in response to a F1 semi-allogeneic in vitro stimulation, (ii) CD4+ T cells purified from 3-week-old tolerant BALB/c mice are able to induce in vitro IgG and IgM production by F1 B cells. Taken together, these results strongly suggest that host CD4+ T cells, belonging to the TH2 subset progressively lose their reactivity towards the F1 semi-allogeneic persistent B cells, reaching a state of unresponsiveness that correlates with the disappearance of serum autoantibodies and autoimmune pathology. 相似文献
16.
Psychopathological dimensions of depression: a factor study of the 17-item Hamilton depression rating scale in unipolar depressed outpatients 总被引:1,自引:0,他引:1
BACKGROUND: Agreement on the factor structure of the Hamilton Depression Rating Scale (HDRS) has not been consistent among studies, and some investigators argued that the scale's factor structure is not reliable. This study aimed at shedding more light on this debated issue. METHODS: We studied 186 adults with unipolar depression (Major Depressive Disorder, n=80; Dysthymic Disorder, n=71; Depressive Disorder Not Otherwise Specified, n=25; Adjustment Disorder, n=10). They had no comorbid DSM-IV axis I or axis II disorders, and had received no treatment with antidepressant drugs in the previous 2 months. The factor structure of the scale was studied using the principal factor method, followed by oblique rotation. Factor scores were computed for each subject using the regression method. RESULTS: Using the scree-test criterion for factor extraction, we obtained a four-factor solution, explaining 43.8% of total variance. The four factors extracted were identified as (1) somatic anxiety/somatization factor; (2) a psychic anxiety dimension; (3) a pure depressive dimension; and (4) anorexia factor. Patients with Major Depressive Disorder scored significantly higher than patients with other diagnoses on the pure depressive dimension. LIMITATIONS: These results need to be replicated in different cultures, using analogous factoring techniques. CONCLUSIONS: Though not exhibiting factorial invariance in the stricter sense of the term, the 17-item HDRS did exhibit a relatively reliable factor structure. Our analysis provides further evidence that the scale is multidimensional. However, as long as the multidimensional character of the scale is taken into account the scale should be able to play a useful role in clinical research. 相似文献
17.
Giulia Barcia Marlne Rio Zahra Assouline Coralie Zangarelli Charles-Joris Roux Pascale de Lonlay Julie Steffann Isabelle Desguerre Arnold Munnich Jean-Paul Bonnefont Nathalie Boddaert Agns Rtig Metodi D. Metodiev Benedetta Ruzzenente 《European journal of human genetics : EJHG》2021,29(3):533
Mitochondrial translation is essential for the biogenesis of the mitochondrial oxidative phosphorylation system (OXPHOS) that synthesizes the bulk of ATP for the cell. Hypomorphic and loss-of-function variants in either mitochondrial DNA or in nuclear genes that encode mitochondrial translation factors can result in impaired OXPHOS biogenesis and mitochondrial diseases with variable clinical presentations. Compound heterozygous or homozygous missense and frameshift variants in the FARS2 gene, that encodes the mitochondrial phenylalanyl-tRNA synthetase, are commonly linked to either early-onset epileptic mitochondrial encephalopathy or spastic paraplegia. Here, we expand the genetic spectrum of FARS2-linked disease with three patients carrying novel compound heterozygous variants in the FARS2 gene and presenting with spastic tetraparesis, axial hypotonia and myoclonic epilepsy in two cases.Subject terms: Metabolic disorders, Mutation 相似文献
18.
Expression of human CD81 in transgenic mice does not confer susceptibility to hepatitis C virus infection 总被引:6,自引:0,他引:6
Masciopinto F Freer G Burgio VL Levy S Galli-Stampino L Bendinelli M Houghton M Abrignani S Uematsu Y 《Virology》2002,304(2):187-196
We previously demonstrated that hepatitis C virus (HCV) binds to human CD81 through the E2 glycoprotein. Therefore, expression of the human CD81 molecule in transgenic mice was expected to provide a new tool to study HCV infection in vivo, as the chimpanzee is the only species currently available as a laboratory animal model that can be infected with HCV. We produced transgenic mice expressing the human CD81 protein in a wide variety of tissues. We confirmed binding of recombinant E2 glycoprotein to the liver tissue as well as to thymocytes and splenic lymphocytes in the transgenic mice. We inoculated chimpanzee plasma infected with HCV into these animals. None of these transgenic animals showed evidence of viral replication. Furthermore, human CD81 transgenic mice that lack expression of endogenous mouse CD81 were also resistant to HCV infection. We conclude that expression of human CD81 alone is insufficient to confer susceptibility to HCV infection in the mouse. The presence of additional possible factors for HCV infection is discussed. 相似文献
19.
Michele Guida Addolorata Casamassima Giulia Monticelli Michele Quaranta Giuseppe Colucci 《Journal of translational medicine》2007,5(1):51
Background and purpose
Metastatic renal cell carcinoma (MRCC) has a poor prognosis with a median overall survival of about one year. Since only a minority of patients experienced therapeutic benefit to current treatments, several studies have attempted to identify factors that may have an impact on response and survival. Cytokines play a crucial role in the host's immune response by regulating the development and function of a lot of biological compartments. Nevertheless, available data on basal cytokine levels in MRCC are very few and no clear profile of serum cytokines has been identified yet in these patients population. Thus, determining the levels of cytokines in MRCC could not only help in understanding the biological mechanisms of the tumor growth, but also in evaluating if different cytokine profiles are correlated with particular clinical behaviors. 相似文献20.
Anthony M Paolo Steven Stites Giulia A Bonaminio Glendon Cox Laura Zeiger Mark Meyer Sandra McCurdy 《Academic medicine》2006,81(9):837-841
PURPOSE: If medical schools increase enrollment to meet anticipated physician shortages, more students from alternate lists will likely be accepted. This study compared the performance of alternate- and main-list students during and one year after medical school. METHOD: The authors assessed admission and performance measures for 1,188 students matriculating from 1997-2003 at the University of Kansas School of Medicine. Measures included Medical College Admission Test scores, basic and clinical science grade point averages, United States Medical Licensing Examination Step 1 and Step 2 scores, residency match information, and residency director ratings. Chi-square analyses, proportional analyses, and independent t-tests were performed. RESULTS: The results indicated that both the admission measures and performance of alternate-list students were generally lower than main-list students, but the differences were small and probably not meaningful. CONCLUSIONS: As long as the applicant pool does not substantially change from its current makeup, increasing enrollment by accepting more students from alternate lists may not adversely affect overall student performance. 相似文献