全文获取类型
收费全文 | 1048篇 |
免费 | 171篇 |
国内免费 | 17篇 |
专业分类
耳鼻咽喉 | 13篇 |
儿科学 | 59篇 |
妇产科学 | 9篇 |
基础医学 | 57篇 |
口腔科学 | 12篇 |
临床医学 | 158篇 |
内科学 | 254篇 |
皮肤病学 | 31篇 |
神经病学 | 44篇 |
特种医学 | 84篇 |
外科学 | 228篇 |
综合类 | 50篇 |
现状与发展 | 20篇 |
预防医学 | 61篇 |
眼科学 | 33篇 |
药学 | 22篇 |
中国医学 | 2篇 |
肿瘤学 | 99篇 |
出版年
2024年 | 7篇 |
2023年 | 44篇 |
2021年 | 20篇 |
2020年 | 25篇 |
2019年 | 26篇 |
2018年 | 62篇 |
2017年 | 51篇 |
2016年 | 37篇 |
2015年 | 47篇 |
2014年 | 57篇 |
2013年 | 75篇 |
2012年 | 35篇 |
2011年 | 47篇 |
2010年 | 57篇 |
2009年 | 65篇 |
2008年 | 36篇 |
2007年 | 25篇 |
2006年 | 28篇 |
2005年 | 19篇 |
2004年 | 15篇 |
2003年 | 18篇 |
2002年 | 18篇 |
2001年 | 21篇 |
2000年 | 12篇 |
1999年 | 23篇 |
1998年 | 29篇 |
1997年 | 33篇 |
1996年 | 43篇 |
1995年 | 29篇 |
1994年 | 17篇 |
1993年 | 21篇 |
1992年 | 7篇 |
1991年 | 8篇 |
1990年 | 10篇 |
1989年 | 12篇 |
1988年 | 15篇 |
1987年 | 14篇 |
1986年 | 11篇 |
1985年 | 13篇 |
1983年 | 6篇 |
1982年 | 8篇 |
1981年 | 4篇 |
1980年 | 5篇 |
1978年 | 12篇 |
1977年 | 9篇 |
1976年 | 7篇 |
1975年 | 5篇 |
1971年 | 4篇 |
1964年 | 5篇 |
1963年 | 4篇 |
排序方式: 共有1236条查询结果,搜索用时 15 毫秒
61.
62.
63.
64.
B M Ghadimi J Uhr T Tucker K Heselmeyer-Haddad G Auer T Ried H Becker 《Zentralblatt für Chirurgie》2001,126(11):922-925
The detection of micrometastases in the bone marrow or peripheral blood of cancer patients is increasingly used for a more sensitive tumor staging and prognostication. The potential value of the currently used techniques for the detection of epithelial antigens by RT-PCR or immunohistochemistry in respect of specificity is currently controversially discussed. In the present study we demonstrate a new approach which enables the direct visualization of the tumor specific alteration of chromosome 8 in circulating tumor cells. We have therefore studied breast cancer patients with various tumor stages and tried to determine the frequency of circulating tumor cells in the peripheral blood by using interphase cytogenetics for chromosome 7 and 8. Imprints of primary breast cancers and cytospins with circulating tumor cells of corresponding patients were studied in a blinded fashion. The blood samples were generated by immunomagnetic enrichment of circulating tumor cells from peripheral blood by ferrofluid and centrifugation onto cover slips. These cytospins were then hybridized with centromer probes 7 and 8. After analyzing 27 patients with benign as well as malignant breast tumors we can demonstrate that the chromosomal pattern between malignant tumor and corresponding circulating tumor cells is identical. Furthermore, the detection of circulating tumor cells directly correlates with the primary tumor stage. We did not find any cells with chromosome 8 alterations in the patients with benign disease. Surprisingly, even in early breast cancers (T1N0) interphase cytogenetics identified circulating tumor cells in 2 out of 4 patients. In conclusion, interphase cytogenetics represent a non-invasive, sensitive and specific assay for the direct visualization of circulating tumor cells in the peripheral blood. The prognostic value of these findings remains to be further evaluated in larger prospective studies. 相似文献
65.
Adam S. Evans Menachem Weiner Prakash A. Patel Elvera L. Baron Jacob T. Gutsche Arun Jayaraman J. Ross Renew Archer K. Martin Ashley V. Fritz Emily K. Gordon Hynek Riha Saumil Patel Kamrouz Ghadimi Eric Guelaff Jared W. Feinman Jillian Dashell Ray Munroe Derek Lauter Harish Ramakrishna 《Journal of cardiothoracic and vascular anesthesia》2018,32(1):1-13
66.
Matti Peura MD ; Jozef Bizik PhD DSc ; Pertteli Salmenperä MSc ; Ariel Noro BM ; Matti Korhonen MD PhD ; Tommi Pätilä MD ; Antti Vento MD PhD ; Antti Vaheri MD PhD ; Riitta Alitalo MD PhD ; Jyrki Vuola MD PhD ; Ari Harjula MD PhD ; Esko Kankuri MD PhD 《Wound repair and regeneration》2009,17(4):569-577
We previously showed cell–cell contacts of human dermal fibroblasts to induce expression of the hepatocyte growth factor/scatter factor (HGF) in a process designated as nemosis. Now we report on nemosis initiation in bone marrow mesenchymal stem cells (BMSCs). Because BMSCs are being used increasingly in cell transplantation therapy we aimed to demonstrate a functional effect and benefit of BMSC nemosis for wound healing. Nemotic and monolayer cells were used to stimulate HaCaT keratinocyte migration in a scratch-wound healing assay. Both indicators of nemosis, HGF production and cyclooxygenase-2 expression, were induced in BMSC spheroids. When compared with a similar amount of cells as monolayer, nemotic cells induced keratinocyte in vitro scratch-wound healing in a concentration-dependent manner. The HGF receptor, c-Met, was rapidly phosphorylated in the nemosis-stimulated keratinocytes. Nemosis-induced in vitro scratch-wound healing was inhibited by an HGF-neutralizing antibody as well as the small molecule c-Met inhibitor, SU11274. HGF-induced in vitro scratch-wound healing was inhibited by PI3K inhibitors, wortmannin and LY294002, while LY303511, an inactive structural analogue of LY294002, had no effect. Inhibitors of the mitogen-activated protein kinases MEK/ERK1/2 (PD98059 and U0126), and p38 (SB203580) attenuated HGF-induced keratinocyte in vitro scratch-wound healing. We conclude that nemosis of BMSCs can induce keratinocyte in vitro scratch-wound healing, and that in this effect signaling via HGF/c-Met is involved. 相似文献
67.
Healing of an arthrodesis occurs optimally when the prepared joint surfaces are held rigidly under compression [1]. We routinely use the “Chisel test” intra-operatively to determine whether we have achieved adequate compression and rigidity after fixation of our foot and ankle fusions. This previously un-reported technique uses tools already on hand when performing an arthrodesis and takes seconds to perform. 相似文献
68.
BM Winklhofer-Roob MA van't Hof DH Shmerling 《Acta paediatrica (Oslo, Norway : 1992)》1995,84(10):1132-1136
The aim of this study was to determine the efficacy of long-term oral β -carotene supplementation for correcting impaired β -carotene status in cystic fibrosis patients. Thirty-five patients (2.3-30.5 years of age) with coefficients of fat absorption of 46-96% (median 88%) received β -carotene 0.5 mg/kg daily and were followed over a 16-month treatment period. Baseline plasma β -carotene concentrations in patients (meanSD, 0.090.06 μ mol/1) were significantly lower than those of age-matched controls (0.860.56 μ mol/1) ( p < 0.0001). Concentrations increased rapidly and reached a plateau at or before 3 weeks that was maintained throughout the study period. Values obtained at 3 weeks (0.890.64 μ mol/1) were significantly higher ( p < 0.0001) than those at baseline and did not differ from controls. Plasma retinol and α -tocopherol concentrations increased during the observation period, but remained within normal ranges. Plasma retinyl palmitate, which was below the detection limit in all but one patient at baseline, did not increase. Thus oral β -carotene supplementation is effective and normalizes β -carotene status of cystic fibrosis patients without evidence of significant side effects. β -Carotene, cystic fibrosis, LDL-cholesterol, oral supplementation, retinol, α-tocopherol 相似文献
69.
MA Hurrell C McLean P Desmond BM Tress A Kaye 《Journal of Medical Imaging and Radiation Oncology》1995,39(1):86-89
A case of malignant granular cell tumour of the sciatic nerve is presented. Computed tomography demonstrated iso-density with muscle and minimal enhancement. Magnetic resonance demonstrated T1 isointensity with muscle with marked enhancement, and isointensity with fat on proton and T2 images. Pathological evidence is presented for its probable Schwann cell histogenesis. 相似文献
70.
BRITT-LOUISE SUNESON RN CHRISTER LINDHOLM MD DMSc ELISABETH HAMRIN RN BM DMSc 《European journal of cancer care》1996,5(1):7-12
The clinical incidence of lymphoedema of the arm in breast cancer patients was studied before and after general mammography screening in the country of jönköping, Sweden. There was a significant decrease of incidence of lymphoedema from 15% in 1983 to 8% in 1988. Clinical and pathological therapy data have been analysed in relation to lymphoedema. Significant factors contributing to lymphoedema were the number of lymph nodes examined and the number of pathologically positive lymph nodes. A higher proportion of patients receiving post-operative radiotherapy to the axilla developed lymphoedema, compared with those with surgery alone.
A comparison between the two groups is impossible due to the fact that post-operative radiotherapy was prescribed only to patients with pathologically positive lymph nodes. 相似文献
A comparison between the two groups is impossible due to the fact that post-operative radiotherapy was prescribed only to patients with pathologically positive lymph nodes. 相似文献