Resistin induces insulin resistance, but does not affect glucose output in rat-derived hepatocytes

AIM: The aim of the present study was to observe the effects of resistin on insulin sensitivity and glucose output in rat-derived hepatocytes. METHODS: The rat hepatoma cell line H4IIE was cultured and stimulated with resistin; supernant glucose and glycogen content were detected. The insulin receptor substrate (IRS)-1 and IRS-2, protein kinase B/Akt, glycogen synthase kinase-3beta(GSK-3 beta), the suppressor of cytokine signaling 3 (SOCS-3) protein content, as well as the phosphorylation status were assessed by Western blotting. Specific antisense oligodeoxynucleotides directed against SOCS-3 were used to knockdown SOCS-3. RESULTS: Resistin induced insulin resistance, but did not affect glucose output in rat hepatoma cell line H4IIE. Resistin attenuated multiple effects of insulin, including insulin-stimulated glycogen synthesis and phosphorylation of IRS, protein kinase B/Akt, as well as GSK-3beta. Resistin treatment markedly induced the gene and protein expression of SOCS-3, a known inhibitor of insulin signaling. Furthermore, a specific antisense oligodeoxynucleotide directed against SOCS-3 treatment prevented resistin from antagonizing insulin action. CONCLUSION: The major function of resistin on liver is to induce insulin resistance. SOCS-3 induction may contribute to the resistin-mediated inhibition of insulin signaling in H4IIE hepatocytes.     

丹红注射液中迷迭香酸、紫草酸、丹酚酸B在大鼠血浆中的浓度测定

目的:建立测定大鼠血浆中丹红注射液中迷迭香酸、紫草酸、丹酚酸B浓度的方法。方法:采用HPLC法,色谱柱为Diamonsil C18柱(250 mm×4.6 mm5,μm);流动相为乙腈-0.4%的磷酸水,梯度洗脱;流速为1.0 mL.min-1;检测波长为288 nm。结果:迷迭香酸在4.0～100.0μg.mL-1浓度范围内线性关系良好(r=0.9925);回收率在85.0%～101.4%之间,日内、日间RSD<8%;紫草酸在4.0～100.0μg.mL-1浓度范围内线性关系良好(r=0.991 1);回收率在84.4%～93.9%之间,日内、日间RSD<9%;丹酚酸B在2.0～200.0μg.mL-1浓度范围内线性关系良好(r=0.998 6);回收率在80.0%～97.4%之间,日内、日间RSD<5%。结论:本文所建立的方法灵敏度好、准确率高,可用于丹红注射液中迷迭香酸、紫草酸、丹酚酸B的药代动力学研究。     

离子色谱直接提取法测定天花粉中二氧化硫的含量

目的：建立离子色谱直接提取法测定天花粉中二氧化硫（SO2）含量的实验方法。方法采用氢氧化钾溶液（25 mmol/L）直接提取,色谱柱为AS11-HC色谱柱（4 mm×250 mm,9.0μm）;柱温20℃;淋洗液：氢氧化钾溶液（20 mmol/L）;淋洗液流速1.00 mL/min;电导检测法,电导池温度20℃通过分离度实验、精密度实验、重复性实验检测并计算分离度、亚硫酸根峰面积和天花粉中SO2含量及亚硫酸根峰面积与进样量的关系。结果亚硫酸根色谱峰与其他相邻色谱峰间有较好的分离度,峰面积变化不大,亚硫酸根平均含量为每克天花粉含SO20.1977 mg、RSD为0.6%。亚硫酸根标准溶液进样量在1.16～29.1μg（即当进样体积为100μL时,被测定溶液浓度为11.6～291.2μg/mL）与亚硫酸根色谱峰的峰面积有良好的线性关系,平均回收率为99.2%,最低定量限以被测定溶液计算为0.00138μg/mL。结论离子色谱法操作简便、准确、快速,适用于天花粉中SO2的定量检测。     

超前镇痛对食管癌手术后应激反应的影响

目的 研究超前镇痛在食管癌手术中镇痛的效果及对术后应激反应的影响.方法 30例食管癌手术病人随机分为超前镇痛组和常规镇痛组,超前镇痛组在切皮前20min硬膜外腔注入0.1mg/mL吗啡 盐酸昂丹司琼8mg 0.15%罗哌卡因混合液15mL,并接镇痛泵(PCEA)(40μg/mL吗啡 盐酸昂丹司琼8mg 0.15%罗哌卡因混合液100mL)持续硬膜外泵入,持续至术后;常规镇痛组术前及术中硬膜外不给予药物,术毕从硬膜外腔导管注入与超前镇痛组配方相同药物,并接PCEA持续硬膜外泵入.于拔管后、拔管后6、12、24和48h各时点对患者进行视觉模拟评分(VAS);采用放射免疫法测定麻醉前、术后90min、术后24h、术后48h血浆胰岛素(Ins)、皮质醇(Cor)以及促肾上腺皮质激素(ACTH).结果 拔管后6.12、24和48h常规镇痛组VAS评分明显高于超前镇痛组.Ins值术后24、48h时点两组均明显高于麻醉前(P<0.05); Cor、ACTH值常规镇痛组明显高于麻醉前,超前镇痛组麻醉后各时点也明显高于麻醉前(P<0.05),组间比较术后24、48h时点两组有显著性差异(P<0.05).结论 超前镇痛用于食管癌手术镇痛明显优于常规镇痛方法,能较好地缓解其术后各种应激反应.     

能谱CT多参数成像评估胆结石成分的应用价值

目的 探究能谱CT多参数成像评估胆固醇结石和非胆固醇结石的应用价值.方法 收集125枚胆结石置入猪肝模型中行CT能谱模式扫描,并重建普通螺旋CT图像,测量所有胆结石单能量40 keV、100 keV时的CT值(HU40 keV、HU100 keV)、有效原子序数(Eff-Z)和普通螺旋CT值(HU120 kVp lik...     

The impact of alfentanil supplementation on the sedation of bronchoscopy: A meta-analysis of randomized controlled trials

Background:The efficacy of alfentanil supplementation for the sedation of bronchoscopy remains controversial. We conduct a systematic review and meta-analysis to explore the influence of alfentanil supplementation on the sedation during bronchoscopy.Methods:We search PubMed, EMbase, Web of science, EBSCO, and Cochrane library databases through December 2019 for randomized controlled trials (RCTs) assessing the effect of alfentanil supplementation versus placebo for the sedation during bronchoscopy. This meta-analysis is performed using the random-effect model.Results:Five RCTs are included in the meta-analysis. Overall, compared with control group for bronchoscopy, alfentanyl supplementation is associated with significantly reduced coughing scores (Std. MD = –0.55; 95% CI = –0.96 to –0.14; P = 0.009) and dose of propofol (Std. MD = –0.34; 95% CI = –0.64 to –0.04; P = 0.03), but reveals the increase in hypoxemia (RR = 1.56; 95% CI = 1.17 to 2.08; P = 0.002).Conclusions:Alfentanyl supplementation benefits to reduce coughing scores and dose of propofol for bronchoscopy, but increases the incidence of hypoxemia. The use of alfentanyl supplementation for bronchoscopy should be with caution.     

组织因子研究进展

组织因子(凝血因子Ⅲ)是一个KD的跨膜糖蛋白,参与外源性凝血途径的启动因子, 一直是研究的热点.随着研究的深入,对其有更多深度和广度的发展.本文就其主要作用及机理、病理生理、抑制剂等方面的最研究进展作一综述.     

白色念珠菌ERG11突变基因在毕氏酵母中的表达

目的 将含有点突变的白色念珠菌编码基因ERG11克隆至毕氏酵母中进行表达,判断其在白色念珠菌对氟康唑敏感性中的作用.方法 将含有点突变的白色念珠菌编码基因ERG11与pPIC3.5K载体重组,构建pPIC3.5K/Ca1.6k重组体,经Top10F'鉴定和筛选,电转入毕氏酵母GS115中,经过诱导表达出现目的 蛋白后,进行药物敏感性试验,确定点突变与耐药的关系.结果 含Y132H突变转化菌株和含Q474K突变的转化菌株对氟康唑的MIC分别上升16倍和8倍,证实其突变与耐药有关.结论 氟康唑耐药可发生于白色念珠菌编码基因ERG11的Y132H与Q474K点突变中.     

HIV感染者皮肤表征及研究进展

皮肤损害往往是HIV感染者最早和最常见症状,通过对文献的分析总结HIV感染者皮肤疾病的发生特点和研究进展。发现近年来的研究偏重于HIV感染者皮肤疾病方面的流行病学,而且都得出较高的发病率。但在皮肤疾病与免疫系统之间的关系研究还不透彻。     

贵州粗壮女贞降血糖作用初探

目的：观察贵州粗壮女贞（Ligustrum robustum BL）降血糖效果。方法：采用尾静脉注射四氧嘧啶造成小鼠糖尿病模型,观察贵州粗壮女贞降血糖作用。结果：贵州粗壮女贞低、中、高剂量组（8g&#183;kg^-1&#183;d^-1、12g&#183;kg^-1&#183;d^-1、24g&#183;kg^-1&#183;d^-1）小鼠给药后血糖值较给药前下降,差异有显著性（P〈0.01）;与糖尿病模型小鼠比较,粗壮女贞不同剂量组的小鼠血糖值均下降,差异有显著性（低剂量组P〈0.05,中、高剂量组P〈0.01）;与阳性对照组比较,高剂量组小鼠血糖值与消渴丸组无差异（P〉0.05）。结论：贵州粗壮女贞对糖尿病模型小鼠具有降血糖作用,高剂量的粗壮女贞降糖效果达到消渴丸的水平。