吸食海洛因患者血浆中心钠素的变化

本文研究了57例吸食海洛因患者血浆ANF浓度变化,并与健康对照组进行比较结果显示海洛因成瘾组显著高于对照组(P<0.01),作者认为测定血浆ANF水平可以作为海洛因对心肌损害的一种判断指标。     

Dissolving agents of human mixed cholesterol stones

Methyl tert-butyl ether which is a powerful cholesterol monohydrate solvent does not completely dissolve mixed cholesterol gallstones when directly infused into the biliary tree. In this work, we compared the effect of various solvents containing different proportions of methyl tert-butyl ether and dimethylsulfoxide in anhydrous and aqueous systems on the in vitro solubilization of human cholesterol stones. The dissolution rates of cholesterol obtained in the presence of methyl tert-butyl ether was markedly decreased when 10 p. 100 water was added. In contrast, the addition of dimethylsulfoxide (30 p. 100) to methyl tert-butyl ether-water system enhanced the stone-solvent contact, improved the cholesterol dissolution rates and left less stone debris. A subsequent dissolution with an alkaline, pH = 8.8, aqueous dimethylsulfoxide-ethylenediaminetetraacetic acid solution strongly reduced the non cholesterol residues. In vivo, nearly complete dissolution of human cholesterol stones implanted in the gallbladder of rabbits was obtained within 8 hours when methyl tert-butyl ether/dimethylsulfoxide (70/30) solvent was infused at a rate of 0.6 ml/h/kg. With methyl tert-butyl ether, only 84 p. 100 of the original stone weight was dissolved. The infusion of these solvents leads to morphological changes in the gallbladder wall with some focal ulcerations. These alterations can be almost completely recovered after two weeks. No histologic evidence of hepatic, duodenal or renal damage was found. We conclude that the mixture methyl tert-butyl ether/dimethylsulfoxide (70/30) constitutes a good solvent for mixed cholesterol stones. Compared with pure methyl tert-butyl ether, the mixed system allows for a more rapid and a more complete dissolution of gallstones.     

胰十二指肠切除术的几点技术改进(附12例报告)

作者对胰十二指肠切除术做了四点技术改进：①经小肠系膜根部后方十二指肠水平段隧道提取空肠至横结肠上区，再做吻合；②经结肠后行十二指肠或胃与空肠端侧吻合术；③放置２５～３０ｃｍ长的胰管支撑内引流管；④经胃窦前壁通过幽门及吻合口放置空肠营养管。文内对上述改进的技术进行了详细的介绍，并阐述了每项技术的优点和意义。     

锌离子对大鼠海马突触体Ca^2＋—Mg^2＋ATP酶活性及蛋白合成的影响

行为实验己经证明，锌过多或缺锌均可影响脑功能。锌作为体内重要的微量元素，影响多种酶的活性及蛋白质和核酸的台成。本实验通过体外分离大鼠脑海马突触体，观察不同浓度锌离子对Ca2 －Mg2 ATP酶的活性和3H－Leu掺入突触蛋白合成的影响．结果表明：1．锌离子浓度在25μmol／L时增加该酶的活性（＜0．01），并促进3H－Leur掺入蛋白质的合成（＜0．05）。2．锌离子在50,100，200μmol／L的较高浓度时对Co2 －M2 ATP酶的活性有显著的抑制作用（分别为：P＜0.05．P＜0．01，P＜0.01），仅200μmol／L对3H—Leu掺入突触蛋白合成有抑制作用。本研究提示：适量的锌对突触体功能的维持是必要的，但剂量过高则起相反作用。     

PROTECTIVE EFFECTS OF PENTAGASTRIN AGAINST STRESS-INDUCED GASTRIC LESIONS IN RATS

The rats, pretreated with pentagastrin, 250 Vg/kg, daily for 7 days, were given restraint water immer sion stress for 3 hours and observed for lesion index of the gastric mucosa. The results showed that the lesion index of the pentagastrin group was sig- nificantly decreased as compared with that of the control group. Light and electron microscopically, the mucosal layer was thickened and both the chief cells and parietal cells were in an active state. This suggests that gastrin has protective effects against the stress-induced gastric lesions through its trophic action on the gastrointestinal tract.     

抗食管癌单克隆抗体及食管癌细胞膜抗原的初步研究

以食管癌“109”细胞株免疫 BALB/C 小鼠,取其脾细胞与小鼠骨髓瘤细胞株SP2/0 融合,经三次克隆化,获得稳定分泌抗食管癌“109”细胞株单克隆抗体的杂交瘤细胞系2GF3。其分泌的单克隆2GF3抗体与外周血淋巴组胞、ABO 型红细胞、癌胚抗原以及3/5胃癌细胞株无反应,与2/5胃癌细胞株有弱(+)反应。与2GF3抗体对应的抗原为2GF3抗原,荧光标记检测表明后者主要表达在细胞膜上。用去污剂提取膜抗原进行电泳后转移至硝酸纤维素膜上进行酶标分析,2GF3抗原是分子量53KD 的糖蛋白,在还原及非还原条件下均显示相同的区带。它与抗癌胚抗原抗体及抗甲胎蛋白抗体均无反应,表明它不同于癌胚抗原和甲胎蛋白。     

楮实对小鼠学习和记忆的促进作用

本试验采用食饵迷宫法和一次训练被动回避性条件反应——跳台法,观察了楮实对小鼠学习和记忆的促进作用。结果表明,楮实对正常小鼠的空间辨别学习、记忆获得有促进作用;可拮抗东莨菪碱造成的记忆获得障碍;改善氯霉素和亚硝酸钠造成的记忆巩固不良;改善30％乙醇引起的记忆再缺损,并对亚硝酸钠中毒缺氧有明显的改善作用。     

赖型钩端螺旋体重组DNApCX7的特异性鉴定和限制性内切酶谱分析

本实验将赖型钩端螺旋体（简称钩体）ＤＮＡ基因库的克隆ｐＣＸ７制备成￣（３２）Ｐ－重组ＤＮＡ探针，对８个不同血清群的１７株问号状钩体、双曲钩体ＰａｔｏｃⅠ株以及细螺旋体３０５５株ＤＮＡ进行打点杂交；同时用１５种ＤＮＡ片断进行限制性内切酶谱分析。结果表明，该重组ＤＮＡ具有问号状钩体种（Ｓｐｅｃｉｅｓ）特异性，但与不同问号状钩体之间的同源性程度有差别；限制性内切酶谱分析发现ｐＣＸ７重组ＤＮＡ片段长约１．７ｋｂ，具有１个Ｂｇ１Ⅱ识别位点和３个ＢｓｔＢ１识别位点。     

广州家禽、家畜批发市场六种动物隐孢子虫感染情况的调查

目的调查广州家禽、家畜批发市场兔、鸭(含雏鸭)、羊、鸡、狗、鸽6种动物的隐孢子虫感染情况。方法取上述动物的粪便292份，用金胺-酚-改良抗酸复染法染色，镜检卵囊。结果检查的6种动物均发现有隐孢子虫感染，自然感染率分别为：64．1％，54．3％(45．0％)，21．7％，20．6％，20．0％，14．6％。结论广州家禽、家畜批发市场6种动物存在隐孢子虫感染。     

尿蛋白-1与糖尿病肾病关系的研究

目的为了探讨尿蛋白-1(UP-)与糖尿病肾病的关系。方法用酶联免疫法测定了90例非胰岛素依赖型糖尿病(NIDDM)患者的UP-1,同时测定尿N-乙酰-β-D-氨基葡萄糖苷酶(NAG)、β2-微球蛋白(β2-mG)。结果糖尿病患者UP-1均较正常对照组增高,UP-1与尿A1b、尿NAG和β2-mG呈正相关。结论在糖尿病早期阶段,肾小管会受到损害,UP-1可作为诊断早期糖尿病肾病的敏感指标。