空、地勤人员血清锌铜钙含量的测定

笔者应用原子吸收分光光度计(AAS)测定了飞行人员(120例)和地勤人员(108人)的血清锌(Zn)、铜(Cu)、钙(Ca)的含量;同时对空、地勤人员分别进行了膳食调查。检测结果:地勤人员血清Zn、Cu含量比较,差异显著(P<0.05),血清 Ca含量则无差异(P>0.05);膳食调查结果飞行人员膳食 Zn摄入量充足,Ca摄入量两组均未达到供给量标准。     

TLR4 mediates human retinal pigment epithelial endotoxin binding and cytokine expression

PURPOSE: It was previously demonstrated that toll-like receptor 4 (TLR4) is involved in species-specific human retinal pigment epithelial (HRPE) photoreceptor outer segment recognition and oxidant production. This study was performed to demonstrate the classical role of TLR4 in HRPE endotoxin (lipopolysaccharide; LPS) binding leading to HRPE proinflammatory cytokine secretion. METHODS: Cultured HRPE cells were examined for TLR4 expression by immunofluorescence, Western blot analysis, and RT-PCR. HRPE cells labeled with fluorescent monoclonal antibodies (mAbs) to TLR4 and its associated adhesion molecule, CD14, were analyzed by real-time microscopy and resonance energy transfer (RET), determining associations of fluorescent LPS, TLR4, and CD14. LPS-induced HRPE secretion of interleukin (IL)-8 was measured with and without specific blocking mAb to TLR4 and CD14. HRPE TLR4 expression was measured after LPS exposure in the presence and absence of blocking antibodies to TLR4 and CD14. RESULTS: All three HRPE cell lines demonstrated constitutive TLR4 expression by immunofluorescence, Western blot analysis, and RT-PCR. Examination of HRPE cells labeled with fluorescent mAb to TLR4, CD14, and LPS demonstrated RET among the three molecules, indicating that LPS-CD14 binding preceded LPS-TLR4 binding and the close association of CD14 and TLR4. LPS-induced IL-8 was significantly reduced (P < 0.05) in the presence of blocking mAb to TLR4 or CD14. Upregulation of HRPE TLR4 gene and protein expression occurred in response to LPS exposure and was inhibited by mAb to TLR4 or CD14. CONCLUSIONS: HRPE TLR4 is a multifunctional molecule that participates in photoreceptor outer segment membrane recognition, oxidant production, LPS recognition, and cytokine production. These roles indicate potential involvement in retinal degenerative and inflammatory processes.     

参麦注射液联合1,6-二磷酸果糖治疗新生儿窒息致心肌损害30例疗效观察

目的 探讨参麦注射液、1,6-二磷酸果糖(FDP)治疗新生儿窒息致心肌损害的疗效.方法 窒息致心肌损害新生儿63例,随机分为治疗组30例和对照组33例,对照组常规治疗,治疗组在常规治疗基础上加用参麦注射液和FDP治疗,1次/d,10d为一个疗程.结果 治疗组心肌酶学恢复时间较对照组显著缩短(P＜0.01).结论 参麦注射液、1,6-二磷酸果糖治疗新生儿窒息后心肌损害具有较好疗效.     

HPLC法测定左乙拉西坦右旋异构体及其他有关物质

摘 要 目的:分别建立手性色谱分离法和RP HPLC法测定左乙拉西坦右旋异构体及其他有关物质。方法: RP HPLC法采用Inertsil C¹⁸色谱柱(250 mm×4.6 mm,5 μm),以乙腈 磷酸氢二钾溶液（10∶90 ）为流动相,流速1 ml·min^-1,检测波长205 nm,柱温30℃;手性色谱分离法采用三（3,5-二甲基苯基氨基甲酸）纤维素酯键合硅胶柱（大赛璐OD-H,250 mm×4.6 mm,5 μm),以正庚烷-无水乙醇-CF-3COOH（83∶17∶0.1）为流动相,流速0.5 ml·min^-1,检测波长为205 nm,柱温30℃。结果:在RP HPLC条件下,已知杂质及破坏产生未知杂质与左乙拉西坦明显分离,左乙拉西坦最低检测限为2 ng;在手性色谱分离法条件下,左乙拉西坦与其右旋对映异构体明显分离,右旋异构体在2～10 μg·ml^-1浓度范围内呈良好线性(r=0.999 5),方法的最低检测限为10 ng。结论:两种方法简便,灵敏,准确,可以有效检测左乙拉西坦右旋异构体及其他有关物质。     

肾移植术后他克莫司神经毒性与CYP3A5基因多态性的相关性研究

目的 总结CYP3A5基因多态性检测与肾移植患者术后他克莫司神经系统毒性的相关性.方法 回顾性分析我院2017年至2020年116例肾移植术后应用他克莫司的患者.采用DNA直接测序法检测患者CYP3A5基因多态性,观察不同基因的患者术后不同时间段的他克莫司药物浓度及神经毒性反应.结果 肾移植术后使用他克莫司的患者神经毒...     

Pulsed High-dose Dexamethasone Improves Interleukin 10 Secretion by CD5+ B Cells in Patients with Primary Immune Thrombocytopenia

Background

B cells expressing CD5 are potentially capable of producing interleukin 10 (IL-10) which contributes to the regulatory function of B cells. This study was aimed at exploring the alteration of numbers of CD5⁺ B cells and their ability of producing IL-10 in patients with immune thrombocytopenia (ITP), and the effects of pulsed high-dose dexamethasone (HD-DXM) therapy on CD5⁺ B cells.

Methods

Peripheral blood mononuclear cells from 25 adult ITP patients were stained with PE-CD5/FITC-CD19 antibodies for flow cytometry analyses before and after HD-DXM therapy. The expression of IL-10 mRNA was measured by RT-PCR. After 24 h culture with or without dexamethasone in the presence of PMA, ionomycin and Brefeldin A, cells were permeabilized and stained with APC-IL-10 antibody to investigate intracellular IL-10 expression. Supernatant IL-10 concentration was detected by ELISA.

Results

The number of CD5⁺ B cells was elevated in patients with ITP. Expression of IL-10 mRNA, percentage of IL-10⁺ cells and intracellular IL-10 in CD5⁺ B cells from untreated patients were significantly higher than that in controls. In contrast, ITP patients showed lower IL-10 concentration in supernatants than controls. After HD-DXM therapy, the number of CD5⁺ B cells decreased to normal level, while intracellular IL-10 expression in CD5⁺ B cells was further enhanced and IL-10 concentration in supernatants was also increased. Similar results were observed when dexamethasone was administrated in vitro.

Conclusions

Increased number of CD5⁺ B cells in which IL-10 is accumulated with decreased IL-10 concentration in supernatants suggests that the ability of CD5⁺ B cells to secret IL-10 is impaired in ITP patients. Both the aberrant number and ability of IL-10 secretion of CD5⁺ B cells could be corrected by HD-DXM.     

地方院校农村大学生成人依恋社会支持与总体幸福感相关性研究

目的探讨地方院校农村大学生成人依恋、社会支持与总体幸福感的相关性,为学校开展有针对性的心理健康教育提供依据。方法对1180名大学生采用亲密关系经历量表、社会支持评定量表和总体幸福感量表进行测评分析。结果农村大学生亲密关系经历量表依恋回避维度评分、社会支持评定量表主观支持维度评分和总体幸福感量表评分均显著高于城市大学生（P〈0．05或0．01）,依恋焦虑维度评分显著低于城市大学生（P〈0．05）;总体幸福感量表评分与社会支持评定量表总分及各维度评分呈显著正相关（P〈0．01）,与亲密关系经历量表各维度评分呈显著负相关（P〈0．01）。农村大学生亲密关系经历量表依恋回避与依恋焦虑维度评分显著影响社会支持评定量表总分及总体幸福感量表评分（P〈0．01）;在农村大学生亲密关系经历量表、社会支持评定量表各维度评分与总体幸福感量表评分的回归分析中,依恋焦虑、主观支持、支持利用度和依恋回避维度评分显著影响总体幸福感量表评分（P〈0．01）,依恋焦虑和依恋回避对总体幸福感的影响水平在加入社会支持后下降。结论农村大学生更倾向于回避型依恋,其依恋水平越低,总体幸福感越强。社会支持在农村大学生的依恋水平和总体幸福感之间起中介作用。     

新辅助化疗对早期巨块型宫颈癌淋巴结转移及生存的意义

目的探讨新辅助化疗(neoadjuvant chemotherapy,NACT)对早期巨块型宫颈癌淋巴结转移及生存的意义。方法回顾性分析165例Ⅰb2～Ⅱa期巨块型宫颈癌患者的病例资料并进行随访,根据术前是否行化疗分为NACT组(n=68)和直接手术组(n=97),比较2组淋巴结转移率及生存情况。结果 NACT及直接手术组发生淋巴结转移的患者转移率差异无统计学意义(13.2%vs 16.5%,P=0.565);但化疗应答者淋巴结转移率则明显低于无应答者(4.8%vs26.9%,P=0.031)。按阳性淋巴结数目计算,NACT组盆腔淋巴结转移率亦低于直接手术组(1.2%vs 2.8%,P=0.002)。截至随访时间止,淋巴结阳性者5年生存率低于淋巴结阴性者(63.0%vs 86.0%,P=0.252)。NACT组5年生存率高于直接手术组(91.0%vs 77.0%,P=0.274)。对化疗应答者5年生存率高于直接手术组(93.0%vs 87.0%,P=0.145)。结论新辅助化疗可以控制宫颈癌淋巴结受累,但对5年生存率及总生存率的影响尚未显示出明显优势。     

A gene-based information gain method for detecting gene–gene interactions in case–control studies

Currently, most methods for detecting gene–gene interactions (GGIs) in genome-wide association studies are divided into SNP-based methods and gene-based methods. Generally, the gene-based methods can be more powerful than SNP-based methods. Some gene-based entropy methods can only capture the linear relationship between genes. We therefore proposed a nonparametric gene-based information gain method (GBIGM) that can capture both linear relationship and nonlinear correlation between genes. Through simulation with different odds ratio, sample size and prevalence rate, GBIGM was shown to be valid and more powerful than classic KCCU method and SNP-based entropy method. In the analysis of data from 17 genes on rheumatoid arthritis, GBIGM was more effective than the other two methods as it obtains fewer significant results, which was important for biological verification. Therefore, GBIGM is a suitable and powerful tool for detecting GGIs in case–control studies.