Time-dependent effects of electroacupuncture at the Ren channel on extracellular signal-regulated kinases 1/2 expression in focal cerebral ischemia rats

BACKGROUND: The onset of focal cerebral ischemia activates extracellular signal-regulated kinases 1 and 2, regulates cell cycle, promotes cell proliferation and differentiation, and affects the normal stage and function of brain cells.OBJECTIVE: To observe the effects of electroacupuncture at the Ren channel on extracellular signal-regulated kinases 1/2 expression in the lateral cerebral ventricle wall of rats with focal cerebral ischemia. The effects were analyzed at different time points after intervention.DESIGN: Randomized controlled study.SETTING: Department of Anatomy, Sun Yat-Sen University. MATERIALS: A total of 60 healthy adult male Wistar rats weighing (250±10) g were provided by the Experimental Animal Center, Medical College of Sun Yat-Sen University. The animal experiment was conducted with confirmed consent by the local ethics committee. The GB6805-Ⅱ electric acupuncture apparatus was provided by Shanghai Medical Equipment High-techno Company. METHODS: The experiment was performed at the Laboratory of Anatomy, Sun Yat-Sen University, from February to July 2007. All experimental animals were randomly divided into the following groups: normal group (n = 6), sham operation group (n = 18), model group (n = 18), and electroacupuncture group (n = 18). Middle cerebral artery occlusion (MCAO) was performed in the model group and electroacupuncture group. Zea Longa's grading standard was used to assess neurological impairment after reperfusion; animals whose grades were between 1 and 4 were included in this study. The normal control group was not exposed to MCAO. In sham operation animals, the right common carotid artery (CCA) was isolated, and the external carotid artery (ECA) was damaged, but no embolism was induced. The electroacupuncture group was given acupuncture on the second day after surgery. The acupoint locations were chosen according to Experimental Acupuncture (People's Publishing House; 1997; First Edition). The Chengjiang, Qihai, and Guanyuan acupoints were labeled and connected to a G6805 electroacupuncture apparatus with sparse-dense waves (sparse waves were 30 Hz, dense waves were 100 Hz), with a frequency of 6-15 V. The duration was 20 minutes. Two days after surgery, the model and sham operation groups were placed with their backs on the operating table, but they received no acupuncture. However, the normal group received acupuncture. The experimental animals under anesthesia were sacrificed on days 7, 14, and 28 post-surgery. Western blot analysis was used to measure expression of extracellular signal-regulated kinases 1/2 in the inferior region of the lateral cerebral ventricle wall. Expression was measured in the normal group at time points corresponding to the sham operation group. MAIN OUTCOME MEASURES: Expression of extracellular signal-regulated kinases 1/2 in the inferior region of the lateral cerebral ventricle wall at different time points after intervention.RESULTS: All 60 rats were included in the final analysis, without any loss. Seven days after MCAO, there was no significant difference in extracellular signal-regulated kinases 1/2 expression in the electroacupuncture group compared to the model group (P > 0.05). However, extracellular signal-regulated kinases 1/2 expression significantly increased in the model group at 14 and 28 days after treatment (P < 0.05).CONCLUSION: Electroacupuncture at the Ren channel can enhance extracellular signal-regulated kinases1/2 expression in the inferior region of the lateral cerebral ventricle wall of rats with focal cerebral ischemia. However, this effect is not apparent until 14 days after electroacupuncture intervention.     

慢性阻塞性肺疾病骨骼肌萎缩机制的研究进展

骨骼肌萎缩是COPD患者常见的全身症状,是导致COPD患者生活质量下降和病死率增加的主要危险因素。COPD骨骼肌萎缩的形成机制非常复杂,与全身炎症反应、氧化应激、缺乏锻炼等因素有关。碳酸酐酶Ⅲ可能也参与了COPD患者骨骼肌萎缩的发生。上述刺激因素会降低骨骼肌氧化代谢能力,使肌蛋白分解／合成过程失衡、影响骨骼肌纤维的凋亡过程及改变肌纤维类型和氧化表型,导致骨骼肌功能障碍。     

HIV／AIDS 患者抗病毒治疗前后心电图分析

目的：分析HIV／AIDS患者抗病毒治疗前后的心电图变化,探讨抗病毒药物对心电活动的影响。方法将130例HIV／AIDS患者按抗病毒治疗时间的长短分为3组,抗病毒治疗时间在1～3年的为A组,4～6年的为B组,7～10年的为C组。对3组患者治疗前后的常规心电图进行比较分析。结果比较抗病毒治疗前后正常心电图、窦性心动过速的发生率及C组异常心电图发生率,差异有统计学意义（ P＜0．05）。 A、B两组异常心电图发生率及ST-T改变、窦性心动过缓、心电轴左偏、左室高电压的发生率抗病毒治疗后较治疗前有所升高,但差异无统计学意义（ P＞0．05）。结论 HIV病毒及高强度的抗病毒治疗会影响心电活动,与患者病程进展及抗病毒治疗时间密切相关。     

开放式颅窗法研究软脑膜微循环方法的建立及应用

本文参照国内外同类颅骨开窗法,用多种实验动物制成了开放式颅窗,可保持实验动物全身及颅窗局部较长时间处于生理状态。首次提出了判定开放式颅窗法是否成功的局部标准,此方法应用的注意事项及使用范围。     

采用超声骨刀在局部麻醉下行外科辅助上颌骨快速扩弓术的临床应用

目的 探讨采用超声骨刀在局部麻醉下进行外科辅助上颌骨快速扩弓（SARME）手术的可行性。方法 选择14例上颌骨横向发育不足的患者（其中男性6例,女性8例）为研究对象,在局部麻醉下用超声骨刀行 SARME手术,手术方式采用腭中缝截骨术+侧方骨皮质切开术+双侧翼颌连接截骨术。采用问卷调查方式调查患者的术中感受。结果 所有患者的SARME手术均在门诊手术室采用局部麻醉完成,患者均耐受并配合手术。超声骨刀损伤小,可以精确截骨,术后反应小。问卷调查结果显示,术中没有或者仅感觉到轻度震动感的有8例（占 57.14%）;术中没有或仅感觉到轻度疼痛和牙齿酸痛的有12例（占85.71%）;对超声骨刀的声音无畏惧感或仅有轻度畏惧感的有 11例（占78.57%）。患者术前和术后6个月牙弓宽度的测量值显示手术扩弓的效果非常明显。结论 利用超声骨刀在局部麻醉下行SARME手术是可行的,但需要更多病例的实践和更长时间的随访。     

重组结核杆菌融合蛋白(EC)用于诊断结核分枝杆菌感染的有效性和安全性系统评价

目的:系统评价重组结核杆菌融合蛋白(EC)相较于结核菌素纯蛋白衍生物(TB-PPD)用于诊断结核分枝杆菌(Mycobacterium tuberculosis,MTB)感染的有效性和安全性。方法:检索临床指南数据库、生物医学文献数据库、卫生行政部门和行业协会官方网站及不良反应监测官方网站,检索时间均自建库截止到2022年2月。英文检索词：Recombinant Mycobacterium tuberculosis fusion protein、CFP10/ESAT6;中文检索词：重组结核分枝杆菌融合蛋白、重组结核杆菌融合蛋白、宜卡、CFP10/ESAT6。收集重组结核杆菌融合蛋白(EC)和结核菌素纯蛋白衍生物(TB-PPD)诊断MTB感染有效性和安全性的指南、共识、团体标准、系统评价和原始研究等。由2名研究者独立筛选文献、提取资料并评价纳入研究的偏倚风险,根据异质性大小采用Meta分析或描述性分析。结果:纳入指南2部、专家共识3篇、团体标准2部,均指出重组结核杆菌融合蛋白(EC)和结核菌素纯蛋白衍生物(TB-PPD)可用于诊断MTB感染和结核病辅助诊断。纳入系统评价1篇,结果显示,重组...     

热损伤对大鼠中枢D_1受体及信号转导的影响

实验大鼠预先分别给予不同药物后置于相同的热环境中,实施热损伤,同时观察大鼠体温升高速率。损伤后立即检测纹状体中 Ｄ_１Ｒ的平衡解离常数和最大结合容量,测定纹状体中ｃＡＭＰ的浓度,检测纹状体细胞中的钙调素（ＣＳＭ）的相对活性,从 Ｄ_１Ｒ及其信号转导的角度,探讨多巴胺受体影响体温调节的机制。结果表明,多巴胺受体激动剂左旋多巴能明显加快动物升温,而Ｄ_１Ｒ桔抗剂ＳＣＨ２３３９０则明显阻止升温;多巴胺两种受体分别具有相互桔抗的促进动物体温升高和阻滞体温升高的作用,神经活性物质神经节昔脂由于其稳定生物膜的作用,而在热损伤模型中同样具有损伤保护作用。     

可移植性人脑胶质瘤组织裸小鼠脑内移植及其MR显像研究

背景与目的:文献报道的胶质瘤动物原位移植模型,大多数是将细胞悬液立体定向接种于鼠脑内,操作繁杂,耗时长,难以在短时间内完成批量实验.本研究接种可移植性人脑胶质瘤组织于裸小鼠脑内,探讨建立人脑胶质瘤裸小鼠原位移植模型及其MR活体成像的可行性.方法:在套管针内,置入位于裸小鼠皮下生长的可移植性人脑胶质瘤组织2 mm3,经微型颅钻钻颅孔后经套管针推入裸小鼠右尾状核内,第30天在配有小鼠专用micro-23微线圈的1.5T MR机上进行头颅扫描和专用软件测量显像的肿瘤体积.继而取全脑作连续冰冻切片,HE染色,在光学显微镜下观察肿瘤病理特征,在体视显微镜目镜下用测微尺测量肿瘤.然后将上述两种方法测得的数据进行统计学分析,评价荷瘤鼠活体MR显像计算肿瘤体积的可行性.结果:经MR扫描的15只颅内接种肿瘤的小鼠,有14只的尾状核区域见到肿瘤显像;在脑切片上,与MR像相同部位见到了肿瘤组织.经脑切片测得的肿瘤体积[(23.19±10.18)mm3]和经MRI测得的肿瘤体积[(23.45±11.64)mm3]比较差异无统计学意义(P＞0.05).肿瘤模型制作成功率为93%(14/15),肿瘤模型MR显像成功率为100%(14/14).结论:经套管针定量植入胶质瘤组织于裸小鼠尾状核,制作可移植性裸小鼠人脑胶质瘤原位模型,具有操作便捷、省时、便于批量制作、致瘤率高等优点.配有小鼠专用微型线圈的1.5T MR机可用于荷瘤裸小鼠的头颅成像、在活体上对移植瘤进行定位和体积测量等研究.     

多学科联合集束化干预对输尿管镜碎石术后感染的效果

目的 探讨多学科联合集束化干预对输尿管镜碎石患者术后感染的效果.方法 选取安徽中医药大学第一附属医院泌尿外科输尿管镜碎石患者60例,按照随机数字表法分为观察组(多学科联合集束化干预)与对照组(常规护理),每组30例,分析并比较两组患者血白细胞(WBC)计数、降钙素原(PCT)、C反应蛋白(CRP)、尿白细胞(LEU)计...