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111.
112.
Bradyarrhythmias and Laparoscopy: A Prospective Study of Heart Rate Changes with Laparoscopy 总被引:1,自引:0,他引:1
Paul S. Myles DipRACOG DA 《The Australian & New Zealand journal of obstetrics & gynaecology》1991,31(2):171-173
Cardiac arrhythmias are a common complication of laparoscopy. Brady-arrhythmias (including asystole) may be life-threatening. The recent introduction of new shorter-acting muscle relaxants (atracurium and vecuronium), with their lack of vagolytic activity, may exacerbate this situation. At the Royal Women's Hospital there have been several episodes of severe bradyarrhythmias and/or asystole associated with these relaxants. This study evaluated heart rate changes in 49 women during laparoscopy: 47% of patients had arrhythmias, 30% of these being bradyarrhythmias. Nearly all the episodes occurred during carbon dioxide insufflation or with traction on pelvic structures. The life-threatening nature of this phenomenon should be understood by all anaesthetists and gynaecologists engaged in laparoscopic procedures. 相似文献
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115.
目的构建可用于高通量筛选JAK/STAT6信号传导通路抑制剂的工程细胞株,建立稳定可靠的筛选方法。方法利用基因重组和转染技术,将STAT6特异性识别启动子IgE基因序列和虫荧光素酶报告基因联合插入pCMV质粒,脂质体法转染至HeLa细胞,经潮红霉素B抗性筛选及报告基因检测,得到稳定表达虫荧光素酶的工程细胞株。通过优化溶剂DMSO浓度,IL4作用浓度及孵育时间等筛选条件,建立了可靠的筛选方法,并在此基础上对1600种化合物进行了筛选。结果建立的筛选方法稳定可靠,系统Z′因子达到0.64。通过对1600种化合物的筛选,得到3个抑制效果较理想的化合物并测得其IC50值。结论所建立的高通量筛选方法可用于JAK/STAT6信号传导通路抑制剂的筛选。 相似文献
116.
目的 探讨脾脏肿瘤的诊断及治疗方法。方法 回顾分析我院 1978年 7月至 1999年 6月手术切除的 2 5例脾脏肿瘤的临床资料。结果 脾脏肿瘤男女发病率相似 ,临床表现以左上腹不适、疼痛及左季肋部肿物多见 ,辅助检查B超诊断率为 6 8% ,CT诊断率为 10 0 % ,良性肿瘤以脾囊肿最常见 ,恶性肿瘤以脾淋巴瘤最常见 ,良性肿瘤的治疗采用脾切除术或脾部分切除术 ,恶性肿瘤行脾切除或联合脏器切除辅以化疗。结论 脾脏肿瘤术前诊断依据CT、B超联合检查 ,最后确诊需手术切除及病理诊断 ,脾脏肿瘤无论良恶性均需手术治疗 相似文献
117.
Michaloudis D Fraidakis O Petrou A Farmakalidou H Neonaki M Christodoulakis M Flossos A Bakos P Melissas J 《Obesity surgery》2000,10(3):220-229
Background: The authors determined prospectively the safety of continuous spinal anesthesia combined with general anesthesia
and the efficacy of postoperative pain relief with continuous spinal analgesia for morbidly obese patients undergoing vertical
banded gastroplasty. Methods: 27 patients (13 men, 14 women) with a mean body mass index (BMI) of 50.4 ± 7.8 and several co-morbidities
were studied. All patients were anesthetized with the same anesthetic regimen, which included midazolam, fentanyl, propofol,
muscle relaxants, N2O, isoflurane and intrathecal bupivacaine. Postoperative pain relief was provided for 5 days and all patients received the
same regimen, which included intrathecal bupivacaine, fentanyl and intravenous tenoxicam. The intrathecal analgesic regimen
was administered continuously through a pump which had the facility of providing bolus doses when requested in predetermined
lockout intervals. Intra-operative monitoring included hemodynamic and respiratory parameters. Additional postoperative monitoring
included respiratory rate, degree of sedation, sensory level of anesthesia, motor response and intensity of pain. Results:
Intraoperative anesthetic technique was safe and provided satisfactory results in the immediate postoperative period. Furthermore,
the postoperative analgesia regimen provided effective analgesia in all patients.The mean doses of fentanyl and bupivacaine
infused intrathecally for the first 24 postoperative hours were 14.1 ± 2.0 μg.h-1 and 0.7 ± 0.1 mg.h-1 respectively, while the requirements of anal gesia decreased progressively with time. The technique provided effective analgesia
with low pain scores, which was reflected by ease in mobilizing and performing physical exercises with the physiotherapist.
Only minor complications related to anesthesia and analgesia were encountered. Conclusion: To our knowledge, this technique
of anesthesia and postoperative analgesia has not been described before in morbidly obese patients. This regimen merits further
controlled trials to establish its place in the perioperative management of morbidly obese patients. 相似文献
118.
Novel expression of the stanniocalcin gene in fish 总被引:2,自引:0,他引:2
119.
目的 观察庆大霉素-德州红耦联物在体外培养小鼠耳蜗Corti器的分布情况,比较不同时间庆大霉素吸收的差异.方法 分离小鼠耳蜗Corti器,体外培养,培养液中加有庆大霉素-德州红耦联物,分别于培养后3 h、6 h、12 h、24 h、48 h、3 d.4 d和7 d收集标本,荧光染色后行激光扫描共聚焦显微镜观察基底膜庆大霉素的分布情况.结果 小鼠耳蜗Corti器在培养3 h后即可见外毛细胞的纤毛和胞体两侧的胞膜有庆大霉素分布;随着培养时间的延长,庆大霉素在Corti器中的所有细胞均见分布,尤以外毛细胞明显,主要聚集在毛细胞顶端纤毛的下方;培养24 h后庆大霉素在外毛细胞的聚集达到最高峰,而在支持细胞未见明显的聚集;体外培养7 d后在毛细胞胞质中仍可见庆大霉素分布.结论 小鼠Corti器体外培养可用来动态检测庆大霉素在耳蜗细胞的吸收和聚集情况. 相似文献
120.
Cytogenetic and molecular genetic analysis of tumorigenic human bronchial epithelial cells induced by radon alpha particles 总被引:4,自引:1,他引:4
To establish a cell culture model for lung carcinogenesis, independent
populations of the human papillomavirus 18-immortalized human bronchial
epithelial cell line BEP2D were treated with high linear energy transfer
radon-simulated alpha-particles, expanded and xenotransplanted into Nu/Nu
mice. Six independent cell lines were established from tumors that
developed from three separate radiation treatments as follows: treatment
(Tx) 1 (30 cGy--two doses), H2BT, Tx 2 (30 cGy-- single dose), R30T1L,
R30T2 and R30T3L, Tx 3 (30 cGy--single dose), H1ATN and H1ATBA1.
Cytogenetic analysis revealed common changes in all tumor lines: loss of
the Y chromosome (ch), one of three copies of ch8, one of three copies of
ch14, and one of two copies of ch4p16-pter and ch11p15-pter. Analysis of
polymerase chain reaction-amplified short tandem repeats of informative
loci confirmed the loss of chY in all lines and loss of heterozygosity
(LOH) at eight loci spanning the length of ch8 in all lines from Tx's 1 and
2. Our data support previous studies indicating the presence of tumor
suppressor genes on ch8. LOH also was confirmed on ch14 at locus D14S306 in
all cell lines from Tx 2 and in one of two lines from Tx 3. This region,
14q12-q13, may contain changes in one of the five known somatostatin
receptor genes (SSTR1). No LOH was detected at any of the informative loci
tested for on ch4 or ch11.
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