全文获取类型
收费全文 | 349篇 |
免费 | 31篇 |
专业分类
儿科学 | 14篇 |
妇产科学 | 10篇 |
基础医学 | 83篇 |
口腔科学 | 4篇 |
临床医学 | 33篇 |
内科学 | 44篇 |
皮肤病学 | 12篇 |
神经病学 | 24篇 |
特种医学 | 6篇 |
外科学 | 77篇 |
综合类 | 4篇 |
一般理论 | 1篇 |
预防医学 | 24篇 |
药学 | 14篇 |
肿瘤学 | 30篇 |
出版年
2023年 | 2篇 |
2022年 | 6篇 |
2021年 | 10篇 |
2020年 | 1篇 |
2019年 | 10篇 |
2018年 | 6篇 |
2017年 | 8篇 |
2016年 | 5篇 |
2015年 | 5篇 |
2014年 | 11篇 |
2013年 | 15篇 |
2012年 | 26篇 |
2011年 | 25篇 |
2010年 | 16篇 |
2009年 | 12篇 |
2008年 | 20篇 |
2007年 | 24篇 |
2006年 | 24篇 |
2005年 | 29篇 |
2004年 | 25篇 |
2003年 | 8篇 |
2002年 | 18篇 |
2001年 | 8篇 |
2000年 | 4篇 |
1999年 | 1篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 2篇 |
1991年 | 4篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1986年 | 2篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 2篇 |
1982年 | 3篇 |
1979年 | 8篇 |
1978年 | 1篇 |
1977年 | 4篇 |
1976年 | 4篇 |
1975年 | 2篇 |
1974年 | 4篇 |
1973年 | 3篇 |
1969年 | 4篇 |
1966年 | 2篇 |
1965年 | 1篇 |
1964年 | 1篇 |
1963年 | 1篇 |
排序方式: 共有380条查询结果,搜索用时 15 毫秒
371.
Simone Punt Willem E. Corver Sander A.J. van der Zeeuw Szymon M. Kielbasa Elisabeth M. Osse Henk P.J. Buermans Cornelis D. de Kroon Ekaterina S. Jordanova Arko Gorter 《Oncotarget》2015,6(36):38681-38694
Cervical cancer is typically well infiltrated by immune cells. Because of the intricate relationship between cancer cells and immune cells, we aimed to identify both cancer cell and immune cell expressed biomarkers. Using a novel approach, we isolated RNA from flow-sorted viable EpCAM+ tumor epithelial cells and CD45+ tumor-infiltrating immune cells obtained from squamous cell cervical cancer samples (n = 24). Total RNA was sequenced and differential gene expression analysis of the CD45+ immune cell fractions identified TCL1A as a novel marker for predicting improved survival (p = 0.007). This finding was validated using qRT-PCR (p = 0.005) and partially validated using immunohistochemistry (p = 0.083). Importantly, TCL1A was found to be expressed in a subpopulation of B cells (CD3−/CD19+/CD10+/CD34−) using multicolor immunofluorescence. A high TCL1A/CD20 (B cell) ratio, determined in total tumor samples from a separate patient cohort using qRT-PCR (n = 52), was also correlated with improved survival (p = 0.027). This is the first study demonstrating the prognostic value of separating tumor epithelial cells from tumor-infiltrating immune cells and determining their RNA expression profile for identifying putative cancer biomarkers. Our results suggest that intratumoral TCL1A+ B cells are important for controlling cervical cancer development. 相似文献
372.
Tinka HOVNIK Darja ?MIGOC SCHWEIGER Primo? KOTNIK Jernej KOVA? Tadej BATTELINO Katarina TREBU?AK PODKRAJ?EK 《Slovenian Journal of Public Health》2015,54(2):98-102
Background
The deficiency of SHOX gene (short stature homeobox-containing gene) has been recognized as the most frequent monogenetic cause of short stature. SHOX gene has been associated with short stature in Turner syndrome and Leri Weill dyschondrosteosis as well with non-syndromic idiopathic short stature. The aim of this study was to determine the frequency of SHOX deletions and mutations in a cohort of Slovenian children with short stature, and to delineate indications for routine SHOX gene mutation screening.Methods and results
40 selected subjects with idiopathic short stature were screened for entire SHOX gene deletion and for mutations in the SHOX gene coding region (exon 2 to 6), together with sequences flanking the exon-intron boundaries. FISH analysis on metaphase and interphase spreads revealed no entire gene deletion. Additionally, no pathogenic point mutations or smaller deletion/duplications were identified in this study group.Conclusions
SHOX gene deletions and point mutations are not a common cause of idiopathic short stature in a cohort of Slovenian children with short stature. Therefore, the frequency of SHOX mutations must be much lower as expected based on the reported data. 相似文献373.
Barbara Robnik Darja Keše Tereza Rojko Alenka Horvat-Ledinek Aleš Pražnikar Bojana Beović 《Journal of infection and chemotherapy》2018,24(4):309-311
Few reports in the literature describe isolated peripheral neuropathies in relation to Mycoplasma pneumoniae infection without concurrent damage to the central nervous system. To our knowledge only a single case of mononeuritis multiplex with brachial plexus neuropathy coincident with M. pneumoniae has been documented until now. Here we present the first clinical case of lobar M. pneumoniae pneumonia in a 19-year-old female patient, where coincident neurological complications manifested as unilateral brachial plexus neuropathy, affecting axillar and suprascapular nerves. Isolated M. pneumoniae from sputum belonged to P1 type 2 and to MLVA type 3-6-6-2. No mutation associated with macrolide resistance in domain V of the 23S rRNA gene was detected. Serological testing of a GM1 antibody showed positive results, which might support the role of immunologic mechanisms in the pathogenesis of peripheral neuropathies related to M. pneumoniae infection. 相似文献
374.
K H Wong W O Schalla R J Arko J C Bullard J C Feeley 《Annals of internal medicine》1979,90(4):634-638
Two antigens were isolated from each of three strains of the Legionnaires' disease (LD) bacterium. One antigen was serotype-specific; the other cross-reacted with strains of LD bacteria of different serotypes. The serotypic antigens contained all the major branched-chain fatty acids characteristic of LD bacteria and were a lipid-protein-carbohydrate complex. Electrophoresis resolved the serotypic antigen of Knoxville 1 strain into four protein bands and one glycoprotein band with molecular weights ranging from 0.5 to 7.2 x 10(5). The specificity of the serotypic antigens was established by immunofluorescent staining, and double gel diffusion confirmed the absence of cross-reactions between the serotypic antigens of different types and the partial identity of the serotypic antigens from serologically related strains. The cross-reacting antigens formed precipitin bands with all the homologous and heterologous sera tested. Induction of immunity by vaccination with serotypic antigens was shown in three animal models using guinea pigs and mice. Passive-transfer of immunity (IgG) was also shown in mice and guinea pigs. These observations raise the possiblity of a vaccine for protection against infection of LD bacteria. 相似文献
375.
376.
377.
Jamnikar Ciglenecki U Grom J Toplak I Jemersić L Barlic-Maganja D 《Journal of virological methods》2008,147(2):257-264
Real-time PCR is an accurate, rapid and reliable method that can be used for the detection and also for the quantitation of specific DNA molecules. The basic principle is the recurring measurement of a fluorescent signal, which is proportional to the amount of amplification product. In our trial two detection systems were tested for classical swine fever virus (CSFV) detection and for its discrimination from other pestiviruses; non-specific dsDNA-binding dye SYBR Green and specific fluorogenic TaqMan MGB probes. Real-time RT-PCR assays were evaluated for diagnostic sensitivity and specificity by different pestiviral reference and field strains. With both approaches, SYBR Green and TaqMan probes, respectively, all of the CSFV strains isolated on cell culture were detected and also clearly distinguished from other pestiviruses. However, the established one-step real-time TaqMan RT-PCR assay was shown to be more appropriate for pestivirus quantitation, it reduces the risk of contamination and is less time consuming. 相似文献
378.
Natalia Pawlas Ulf Strömberg Bo Carlberg Milena Cerna Florencia Harari Raúl Harari Milena Horvat Frantiska Hruba Kvetoslava Koppova Andrea Krskova Mladen Krsnik Yu-Feng Li Lina Löfmark Thomas Lundh Nils-Göran Lundström Badiaâ Lyoussi Iwona Markiewicz-Górka Darja Mazej Josko Osredkar Krystyna Pawlas Gerda Rentschler Vera Spevackova Zdravko Spiric Anneli Sundkvist Janja Snoj Tratnik Draženka Vadla Soumia Zizi Staffan Skerfving Ingvar A. Bergdahl 《International journal of occupational medicine and environmental health》2013,26(1):58-72
Objectives
The aim of the study was to make an international comparison of blood levels of cadmium (B-Cd), lead (B-Pb) and mercury (B-Hg) of women in seven European, and three non-European cities, and to identify determinants.Materials and Methods
About 50 women (age: 46–62) from each city were recruited (totally 480) in 2006–2009. Interview and questionnaire data were obtained. Blood samples were analysed in one laboratory to avoid interlaboratory variation.Results
Between the European cities, the B-Pb and B-Cd results vary little (range of geometric means: 13.5–27.0 μg/l and 0.25–0.65 μg/l, respectively); the variation of B-Hg was larger (0.40–1.38 μg/l). Between the non-European cities the results for B-Pb, B-Cd and B-Hg were 19.2–68.0, 0.39–0.99 and 1.01–2.73 μg/l, respectively. Smoking was a statistically significant determinant for B-Cd, while fish and shellfish intakes contributed to B-Hg and B-Pb, amalgam fillings also contributed to B-Hg.Conclusions
The present results confirm the previous results from children; the exposure to lead and cadmium varies only little between different European cities suggesting that other factors than the living area are more important. The study also confirms the previous findings of higher cadmium and lead levels in some non-European cities. The geographical variation for mercury is significant. 相似文献379.