亚硝酸钠与AFB1相互作用对遗传物质影响的实验研究

以正常人外周血淋巴细胞的ＳＣＥ率作为细胞遗传学指标，研究了Ｎａ＿２ＳｅＯ＿３与ＡＦＢ＿１相互作用对细胞遗传物质的影响。结果表明，一定浓度的Ｎａ＿２ＳｅＯ＿３（１０￣（－５）ｍｏｌ）对ＡＦＢ＿１所诱发的ＳＣＥ有明显的抑制作用，但当浓度达到１０￣（－２）ｍｏｌ时，细胞增殖受到抑制，到１０￣（－１）ｍｏｌ时细胞出现毒性现象。提示Ｎａ＿２ＳｅＯ＿３具有抑变和细胞毒性双相作用。所以在Ｎａ＿２ＳｅＯ＿３与ＡＦＢ＿１相互作用的ＳＣＥ实验中应避免Ｎａ＿２ＳｅＯ＿３的浓度过高而损伤培养的细胞。     

钙和鸡冠花黄酮提取物对大鼠废用性骨质疏松的作用

目的探讨钙和鸡冠花黄酮提取物对废用性骨质疏松大鼠的作用,为骨质疏松的防治提供依据.方法选用健康SD大鼠40只,雄性.随机分为5组,第1、2组分别为正常对照和阳性对照,灌胃蒸馏水5 ml/(kg@d),第2～5组制造废用性骨质疏松大鼠模型,第3、4、5组为预防组,分别灌胃等量鸡冠花提取物,碳酸钙,鸡冠花提取物加钙溶液.实验8周后,测骨密度(BMD),股骨CT值,测血清MDA、SOD及24 h尿钙、羟脯氨酸(HOP)、尿肌酐含量等.结果阳性组与正常对照组比较股骨重、灰分重、CT值、血清钙下降,尿钙、尿HOP升高(P＜0.05或0.01),预防组与阳性组比较股骨重、CT值显著增加,碳酸钙组、鸡冠花加钙组灰分重显著增加(P＜0.05).与阳性组比较各预防组血清钙增加,尿钙、尿HOP减少(P＜0.05或＜0.01).鸡冠花组、鸡冠花加钙组与阳性组比较股骨BMD、血清SOD升高,MDA降低,单纯补钙组仅BMD升高(P＜0.05或＜0.01).结论鸡冠花提取物、钙对废用性骨质疏松大鼠骨质代谢均有促进作用,两者合用具有更好的预防效果,能延缓或减轻废用性骨质疏松的发生.     

珊瑚型人工髋关节置换术的临床随访及松动原因的分析

自１９８３年至今应用珊瑚型人工髋关节置换术共５４例６０髋。其中人工全髋关节置换术３４例４０髋，人工股骨头置换术２０例２０髋。在５４例６０髋珊瑚型人工髋关节置换术中，有３７髋随访６个月～１３年，平均随访时间约７年，疗效满意率为８５．１％，其中假体松动４例占１４．８％，髋臼松动２例７．４％。假体松动原因是假体与股骨负重界面之间没有达到稳定接触，假体与髓腔形状不相匹配，修整髓腔松质骨时，髓腔扩大器应比假体小一号，避免假体与界面存留缝隙。对于髋臼发育不良的患者应避免髋关节旋转中心向外侧移位，应向内上方加深髋臼以减少水平移位距离，其次髋臼植骨加盖勿在负重区。     

A study on various symptoms of chronic hepatitis treated with radix astragali and its regulative effect to levels of serum hormone

Sixty-seven cases of chronic hepatitis were treated with Radix Astragali. After treatment (2-month course), the clinical improvement rate in 38 cases of the Stagnation of the Liver-Qi and Deficiency of the Spleen type was 92.1%, and in 26 cases of the Deficiency of Liver Yin and Kidney Yin type was 88.5%, more effective than in the control group (P<0.05). The regulative effect to the levels of serum hormone was observed in the patients of the Stagnation of the Liver-Qi and Deficiency of the Spleen type treated with this medicine. The results showed that the levels of serum triiodothyronine, estradiol (female) and testosterone (male) were increased after treatment (1.40±1.38 ng/dl, 129.30±1.23 pg/ml and 496.24±1.47 ng/dl). Pre-treatment levels were 1.22±1.49 ng/dl, 104.60±1.45 pg/ml and 398. 17±1.55 ng/dl respectively (P<0.05); however, the level of serum prolactin (2.75±4.46 ng/ml) was lower after treatment than before treatment (3.20±3.82 ng/ml,P<0.05). No obvious changes were observed in the levels of serum follicle stimulating hormone, luteinizing hormone, thyroid stimulating hormone, thyroxine, triiodothyronine uptake ratio and cortisol after treatment.     

肝尾叶切除

肝尾叶深藏于肝门结构与下腔静脉之间，具有独立的管道系统，手术切除风险较大，作者结合２例肝尾叶切除的体会，复习文献，对肝尾叶的解剖、影像学特征、切除指征及手术方法进行了讨论。     

亲电性对醌二甲烷与富电子烯烃的电荷转移聚合

概述了亲电性对醌二甲烷与富电子烯烃电荷转移聚合的最新进展，着重介绍聚合的一般特征、引发机理、链增长机理，以及单体结构、溶剂对聚合反应的影响。     

大肠癌组织p53基因突变及其与临床病理关系的探讨

应用非同位素标记ＰＣＲ－ＳＳＣＰ技术对３４例大肠癌组织ｐ５３基因第五外显子突变进行了检测。结果：５０％（１１／２２）结肠癌，８．３％（１／１２）直肠癌存在ｐ５３基因突变，低分化腺癌高于高、中分化腺癌（Ｐ＜０．０５），ＤｕｋｅｓＢ、Ｃ期高于ＤｕｋｅｓＡ期（Ｐ＜０．０５），ｐ５３基因第五外显子突变在已发生淋巴转移的大肠癌组织中呈现较强的趋势。提示：检测结肠癌原发灶ｐ５３基因第五外显子突变，对指导结肠癌的诊断、治疗及预后判断具有一定意义     

壮骨肾宝对实验性小鼠高脂血症的影响

目的观察由黄芪、白术、淫羊藿组成的复方制剂(壮骨肾宝)对实验性小鼠高脂血症的影响。方法用蛋黄乳液快速致小鼠单纯性高胆固醇血症和喂养高脂饲料致高脂血症模型 ,观察预防性给予不同剂量的壮骨肾宝后对血清总胆固醇(TC)、甘油三酯(TG)和高密度脂蛋白胆固醇(HDL -ch)的影响。结果壮骨肾宝中、高剂量能使血清TC降低(P<0.05),HDL -ch升高(P<0.01) ,但对TG的影响不大。结论壮骨肾宝有一定降血脂作用 ,并与剂量有关     

示波极谱法测定环境中痕量叶蝉散

本文研究了叶蝉散的水解产物邻异丙基酚在硼酸-NaOH－EDTA底液中与MBTH偶合生成物，在电位-0.35V（VS、S、C、E）处的极谱行为，加入等体积的乙醇可提高方法灵敏度和稳定性。方法灵敏度高，较比色法高一个多数量级；线性范围广，0．04～1．5PPm与峰高成正比；合并变异系数4.1％；平均回收率96％～99．2％；方法操作简便、快速，干扰少；样品分析结果与比色法无差异。     

Treatment of hemophilia A by living mother-to-son splenic transplantation. First case report in the world.

A case of severe hemophilia A was treated by living mother-to-son splenic transplantation. The recipient was a 9-year-old boy. After splenic transplantation, the level of blood factor VIII:C was increased, and no spontaneous hemorrhage occurred. The boy was followed up for over two years. This is a case of spleen allotransplantation with the longest function in the world.