全文获取类型
收费全文 | 369篇 |
免费 | 22篇 |
国内免费 | 14篇 |
专业分类
儿科学 | 17篇 |
妇产科学 | 9篇 |
基础医学 | 59篇 |
口腔科学 | 9篇 |
临床医学 | 29篇 |
内科学 | 79篇 |
皮肤病学 | 5篇 |
神经病学 | 4篇 |
特种医学 | 126篇 |
外科学 | 19篇 |
综合类 | 7篇 |
预防医学 | 9篇 |
眼科学 | 2篇 |
药学 | 18篇 |
肿瘤学 | 13篇 |
出版年
2019年 | 1篇 |
2018年 | 1篇 |
2017年 | 1篇 |
2016年 | 2篇 |
2015年 | 6篇 |
2014年 | 4篇 |
2013年 | 7篇 |
2012年 | 3篇 |
2011年 | 6篇 |
2010年 | 5篇 |
2009年 | 15篇 |
2008年 | 8篇 |
2007年 | 15篇 |
2006年 | 8篇 |
2005年 | 7篇 |
2004年 | 8篇 |
2003年 | 2篇 |
2002年 | 2篇 |
2001年 | 6篇 |
2000年 | 5篇 |
1999年 | 9篇 |
1998年 | 21篇 |
1997年 | 24篇 |
1996年 | 23篇 |
1995年 | 10篇 |
1994年 | 16篇 |
1993年 | 7篇 |
1992年 | 8篇 |
1991年 | 2篇 |
1990年 | 6篇 |
1989年 | 11篇 |
1988年 | 9篇 |
1987年 | 12篇 |
1986年 | 12篇 |
1985年 | 14篇 |
1984年 | 5篇 |
1983年 | 17篇 |
1982年 | 18篇 |
1981年 | 15篇 |
1980年 | 11篇 |
1979年 | 6篇 |
1978年 | 6篇 |
1977年 | 12篇 |
1976年 | 6篇 |
1975年 | 2篇 |
1973年 | 2篇 |
1972年 | 4篇 |
1971年 | 1篇 |
1969年 | 3篇 |
1966年 | 1篇 |
排序方式: 共有405条查询结果,搜索用时 15 毫秒
61.
62.
63.
64.
65.
66.
We have examined the morphological and secretory behavior of rat and guinea pig megakaryocytes exposed for up to 24 hours to extracellular matrix produced by cultured bovine endothelial cells. By phase-contrast microscopy of living cells and in more detail by scanning electron microscopy, the megakaryocytes showed a nonreversible adherence, an extensive formation of filopodia around the periphery like the rays of the sun, and a tendency toward flattening. These filopodia were generally linear with attenuated tips and were larger than, but resembled the filopodia of, rat or guinea pig platelets exposed to this extracellular matrix. In contrast, isolated megakaryocytes on glass or on uncoated plastic surfaces did not show these responses; adherence, in the face of gentle agitation before fixation, was minimal, with rare filopodia and no flattening. Megakaryocytes that interacted with the extracellular matrix produced significant amounts of thromboxane A2, but this did not occur on uncoated surfaces and could not be attributed to other contaminating cells in the megakaryocyte suspensions. The appearance in megakaryocytes of these typical platelet responses indicates that megakaryocytes acquire the functional capabilities of platelets by the synthesis and assembly of platelet substances and organelles. Thromboxane production by megakaryocytes stimulated by the extracellular matrix is a readily quantifiable measure of this capacity. 相似文献
67.
68.
Fávaro WJ Nunes OS Seiva FR Nunes IS Woolhiser LK Durán N Lenaerts AJ 《Infectious agents and cancer》2012,7(1):14-15
Background
Compounds that can act as agonists for toll-like receptors (TLRs) may be promising candidates for the development of drugs against infectious diseases and cancer. The present study aimed to characterize the immunomodulatory effects of P-MAPA on TLRs in vitro and in vivo, as well as to investigate its potential as adjuvant therapy in infectious diseases and cancer.Methods
For these purposes, the activity of P-MAPA on TLRs was assayed in vitro through NF-??B activation in HEK293 cells expressing a given TLR, and using an in vivo animal model for bladder cancer (BC). The antimicrobial activity of P-MAPA was tested against Mycobacterium tuberculosis (TB) in vitro in an MIC assay, and in vivo using an aerosol infection model of murine tuberculosis. Antitumor effects of P-MAPA were tested in an animal model with experimentally induced BC. Moxifloxacin (MXF) and Bacillus Calmette-Guerin (BCG) were used as positive controls in the animal models.Results
The results showed that P-MAPA, administered alone or in combination with MXF, induced significant responses in vivo against TB. In contrast, the compound did not show antimicrobial activity in vitro. P-MAPA showed a significant stimulatory effect on human TLR2 and TLR4 in vitro. In BC, TLR2, TLR4 and p53 protein levels were significantly higher in the P-MAPA group than in the BCG group. The most common histopathological changes in each group were papillary carcinoma in BC group, low-grade intraepithelial neoplasia in BCG group and simple hyperplasia in P-MAPA group. Concerning the toxicological analysis performed during BC treatment, P-MAPA did not show evidence for hepatotoxicity and nephrotoxicity.Conclusions
In conclusion, P-MAPA acted as TLR ligand in vitro and improved the immunological status in BC, increasing TLR2 and TLR4 protein levels. P-MAPA immunotherapy was more effective in restoring p53 and TLRs reactivities and showed significantly greater antitumor activity than BCG. The activation of TLRs and p53 may provide a hypothetical mechanism for the therapeutic effects in both cancer and infectious diseases. Taken together data obtained will encourage the further investigation of P-MAPA as a potential candidate for the treatment of cancer and infectious diseases. 相似文献69.
70.
Experience with percutaneous drainage in four cases of splenic abscess is presented. Percutaneous drainage and antibiotics were curative in three of four cases. The one case not adequately treated by antibiotics and percutaneous drainage was also complicated by ascites and multilocular collections. Percutaneous drainage in this case did not adversely affect subsequent curative surgery. Percutaneous catheter drainage of splenic abscess under radiologic guidance can be a safe, effective treatment. 相似文献