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31.
Nationwide epidemiology and prognosis of dialysis‐requiring acute kidney injury (NEP‐AKI‐D) study: Design and methods 下载免费PDF全文
Chih‐Chung Shiao Pei‐Chen Wu Vin‐Cent Wu Jui‐Hsiang Lin Heng‐Chih Pan Ya‐Fei Yang Tai‐Shuan Lai Tao‐Min Huang Che‐Hsiung Wu Wei‐Shun Yang Chih‐Jen Wu Chih‐Chin Kao Chiao‐Yin Sun Chun‐Te Huang Kuo‐Hua Lee Chan‐Yu Lin Te‐Chuan Chen Fu‐Chang Hu Hung‐Hsiang Liou Kuo‐Cheng Lu Kuo‐Liong Chien Jian‐Jhong Wang Wei‐Chih Kan Feng‐Chi Kuo Hugo You‐Hsien Lin Cheng‐Min Chen Zi‐hong You Jen‐Pi Tsai Chih‐Jen Weng Hung‐Yuan Chen Chao‐Fu Chang Wen‐Ding Hsu Mai‐Szu Wu Chiu‐Ching Huang Kwan‐Dun Wu 《Nephrology (Carlton, Vic.)》2016,21(9):758-764
32.
Background
A common hypothesis is that caregiving is deleterious to women’s mental health. International studies continue to emphasize the importance of mental health issues for women. Yet only a few researchers have used population-based surveys to explore the association between caregiving and depressive symptomatology in the context of the community, and even less is known about this aspect of Baby Boomer women in a global context.Methods
The present study uses eight international surveys covering nineteen nations (N?=?15,100) and uses multilevel logistic models to examine possible linkages between caregiving and the likelihood of depressive symptoms among Baby Boomer women, when taking individual-level and country-level social factors into consideration.Results
The various analyses found a significant variation in the likelihood of depressive symptoms among these Boomer women across the nations investigated and across both individual-level and country-level characteristics. The significant association of caregiving by women and the likelihood of depressive symptoms is related to their social status in some nations (OR?=?1.30; p?<?0.001). Boomer women living in countries with high rates of female participation in managerial/professional work (OR?=?1.04; p?<?0.05) and living in countries where women are often in vulnerable employment (OR?=?1.01; p?<?0.05) are at greater risk of depressive symptomatology.Conclusions
These findings demonstrate that the depressive consequences of caregiving by women are, to some degree, contingent upon social context and structure. Policies aimed at promoting mental health among female Baby Boomers should therefore be context specific.33.
Rohan PJ Hughes MOptom BVisSc Stephen J Vincent PhD BAppSc Scott A Read PhD BAppSc Michael J Collins PhD MAppSc DipAppSc 《Clinical & experimental optometry》2020,103(1):68-85
Evidence from animal and human studies suggests that ocular growth is influenced by visual experience. Reduced retinal image quality and imposed optical defocus result in predictable changes in axial eye growth. Higher order aberrations are optical imperfections of the eye that alter retinal image quality despite optimal correction of spherical defocus and astigmatism. Since higher order aberrations reduce retinal image quality and produce variations in optical vergence across the entrance pupil of the eye, they may provide optical signals that contribute to the regulation and modulation of eye growth and refractive error development. The magnitude and type of higher order aberrations vary with age, refractive error, and during near work and accommodation. Furthermore, distinctive changes in higher order aberrations occur with various myopia control treatments, including atropine, near addition spectacle lenses, orthokeratology and soft multifocal and dual-focus contact lenses. Several plausible mechanisms have been proposed by which higher order aberrations may influence axial eye growth, the development of refractive error, and the treatment effect of myopia control interventions. Future studies of higher order aberrations, particularly during childhood, accommodation, and treatment with myopia control interventions are required to further our understanding of their potential role in refractive error development and eye growth. 相似文献
34.
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36.
Plasma levels of plasminogen activator inhibitor type-1 (PAI-1), beta- thromboglobulin (beta TG), and fibrinopeptide A (FPA) were followed over 24 hours in 30 patients treated with alteplase for acute myocardial infarction. Samples were taken at baseline (T Oh), after 90 minutes (under alteplase, no heparin, T 1.5h), after 120 minutes (under alteplase and heparin, T 2h), 30 minutes after thrombolytic therapy (T 3.5h), as well as 12 hours (T 12h) and 24 hours (T 24h) after baseline. PAI-1 antigen levels (55 +/- 9 ng/mL at T Oh, mean +/- SEM) decreased to 35 +/- 5 (T 1.5h) and 40 +/- 6 (T 2h) ng/mL under alteplase, before increasing to 84 +/- 22 (T 3.5h), 130 +/- 30 (T 12h), and 64 +/- 7 (T 24h) ng/mL after therapy, P less than .001. A high baseline PAI-1 activity (18 +/- 3 ng/mL) decreased to 2.0 +/- 0.4 (T 1.5h) and 1.7 +/- 0.2 (T 2h) under alteplase and increased to 32 +/- 5 (T 12h) and 19 +/- 3 (T 24h) ng/mL after therapy (P less than .0001). beta TG levels (339 +/- 105 ng/mL at T Oh) decreased to 203 +/- 48 (T 2h), 154 +/- 51 (T 3.5h), 187 +/- 40 (T 12h), and 142 +/- 32 (T 24h) ng/mL under heparin (P less than .01). FPA levels (34 +/- 9 ng/mL at T Oh) increased to 85 +/- 15 ng/mL under alteplase alone (T 1.5h) and normalized under heparin (11 +/- 4, 6 +/- 2, 4 +/- 2, and 3 +/- 1 ng/mL at T 2h, T 3.5h, T 12h, and T 24h, respectively). A high level of FPA at T 3.5h correlated with reocclusion (33 +/- 12 ng/mL, n = 4 v 2.9 +/- 0.5 ng/mL, n = 21, P less than .005). We conclude that plasma levels of PAI- 1 antigen as well as activity markedly increase after alteplase therapy of acute myocardial infarction. The high activity of PAI-1 and decreasing beta TG levels suggest that platelets do not contribute significantly to this phenomenon. The marked increase of FPA levels under recombinant tissue-type plasminogen activator alone and its normalization under heparin emphasize the important role of concomitant anticoagulation in controlling further intravasal fibrin generation under alteplase. 相似文献
37.
The growth factor requirements of STRO-1-positive human bone marrow stromal precursors under serum-deprived conditions in vitro 总被引:9,自引:2,他引:9
Factors that regulate the growth and development of primitive bone marrow stromal cell precursors are not well defined. We have examined 25 purified recombinant growth factors for their ability to initiate and support clonogenic growth of fibroblast colony-forming cells (CFU- F) from adult human bone marrow. Assays were performed using bone marrow mononuclear cells (BMMNC) enriched in CFU-F by magnetic- activated cell sorting (MACS) using the monoclonal antibody (MoAb) STRO- 1. A serum-deprived assay was developed to avoid components of fetal calf serum (FCS) that may mask or otherwise modify the response of CFU- F to exogenously added factors. L-ascorbate and the glucocorticoid dexamethasone were found to be essential for CFU-F colony development under serum-deprived conditions. Importantly, clonogenic growth of CFU- F in this culture system was absolutely dependent on an exogenous source of growth factor. Platelet-derived growth factor-BB (PDGF) and epidermal growth factor (EGF) demonstrated the greatest ability to support colony growth. Colony formation was dose-dependent, with half- maximal colony numbers at approximately 0.2 ng/mL for either factor and plateau numbers at concentrations in excess of 1.0 ng/mL. Simultaneous addition of PDGF and EGF had no effect on the number of colonies initiated but resulted in dose-dependent increases in mean colony diameter that were significant (P < or = .05) when compared with the effect of either factor alone or with the size of colonies elicited in control cultures by 20% FCS. Fluorescence-activated cell sorting (FACS) of BMMNC using MoAbs to the alpha chain of the PDGF receptor and to the EGF receptor in combination with the Moab STRO-1 demonstrated constitutive expression of both receptors by greater than 90% on CFU-F. Receptors for insulin-like growth factor-1 (IGF-1) and nerve growth factor (NGF) were also detected on STRO-1+ CFU-F, but in vitro both IGF- 1 and NGF did not support colony growth. This report demonstrates the development of a simple, reproducible, and stringent culture system for the growth and assay of stromal precursors under serum-deprived conditions and represents an important prerequisite for future studies of the role of growth factors in the regulation of stromal cell proliferation, differentiation, and development. 相似文献
38.
Human leukemia cell maturation induced by a T-cell lymphokine isolated from medium conditioned by normal lymphocytes. 总被引:2,自引:2,他引:2 下载免费PDF全文
K Leung J W Chiao 《Proceedings of the National Academy of Sciences of the United States of America》1985,82(4):1209-1213
Human myelogeneous leukemia cells in liquid culture can be induced to mature along the monocyte/macrophage pathway by a maturation inducer derived from the conditioned medium of activated human T lymphocytes. Serum-free conditioned medium was used for the isolation of the T-cell lymphokine. The maturation inducer was purified approximately equal to 6000-fold by ammonium sulfate precipitation, low-salt elution from DEAE-Sepharose CL-6B, gel filtration on Bio-Gel A-0.5m, and NaDodSO4/PAGE under nonreducing conditions. The molecular weight of the maturation inducer was 36,000-58,000 on NaDodSO4/PAGE. Terminal differentiation associated with inhibition of leukemia cell proliferation and expression of mature cell properties was observed with the isolated maturation inducer, identical to the activity observed with the unfractionated conditioned medium. Cell-cycle analysis revealed that the proportion of replicating S-phase cells was reduced from 40% to 7% after initial interaction of the maturation inducer with cells. The differentiating cells simultaneously acquired monocyte antigen, membrane complement receptors, phagocytic function, and monocyte/macrophage morphology. The maturation-inducing activity is dose-dependent, with more inducer causing the development of more mature cells in a shorter time period. The maturation inducer was shown to be stable after pH 2 treatment, independent of interleukin 2 and colony-stimulating factor, devoid of alpha-, beta-, and gamma-interferon, and not affected by antibody to interferon. The maturation inducer may play a role as a physiological regulator of monocytic and leukemia cell development. 相似文献
39.
Proliferation of myeloid progenitor cells in human long-term bone marrow cultures is stimulated by interleukin-1 beta 总被引:3,自引:0,他引:3
Fibbe WE; Goselink HM; Van Eeden G; Van Damme J; Billiau A; Voogt PJ; Willemze R; Falkenburg JH 《Blood》1988,72(4):1242-1247
To study the effect of interleukin-1 (IL-1) beta on the proliferation of hematopoietic progenitor cells (HPC) in long-term bone marrow cultures (LTBMC), stromal cell layers were established from normal human bone marrow. Autologous cryopreserved mononuclear phagocyte- and T-lymphocyte-depleted bone marrow cells were reinoculated on the stromal layers in fresh culture medium, with or without the addition of human IL-1 beta (30 U/mL). Once a week, half of the culture supernatant was replaced with fresh culture medium with or without IL-1, and all nonadherent cells were returned to the flasks. At weekly intervals during a period of 5 weeks, one culture was sacrificed to determine the total number of cells and hematopoietic progenitor cells, present in the adherent and the nonadherent cell fractions. In IL-1-stimulated cultures, the number of cells recovered during a period of 5 weeks exceeded the number of cells in unstimulated control cultures by 1.5 times. This difference was attributed to a twofold increase in the number of adherent cells. The number of HPC recovered from IL-1- stimulated cultures was not different from that recovered from controls. The levels of colony-stimulating activity (CSA) in supernatants from IL-1-stimulated cultures were significantly higher than those in supernatants from control cultures. These results indicate that IL-1 enhances the recovery of cells in LTBMC by stimulating the proliferation of HPC with the concurrent release of CSA from stromal cells, without diminishing the number of HPC. 相似文献
40.
Chia‐Ming Yeh Ming‐Ju Hsieh Jia‐Sin Yang Shun‐Fa Yang Yi‐Ting Chuang Shih‐Chi Su Meng‐Yuan Liang Mu‐Kuan Chen Chiao‐Wen Lin 《Environmental toxicology》2019,34(10):1085-1093
Geraniin has been reported to have numerous biological activities, including antiviral, antihypertensive, antihyperglycaemic, liver protective, antidiabetic, and apoptotic activities. However, the anti‐migration effects of geraniin on oral cancer remain elusive. In this study, we revealed the potential antitumor mechanisms of geraniin through the inhibition of the migration and invasion of human oral cancer cell lines SCC‐9 and SCC‐14. The results of gelatin zymography and Western blot assays revealed that geraniin significantly reduced the activity and expression of matrix metalloproteinase‐2 (MMP‐2) of oral cancer cells in a concentration‐dependent manner. Furthermore, geraniin potently suppressed the phosphorylation of focal adhesion kinase (FAK), Src, and extracellular signal‐regulated kinase (ERK)1/2 but did not affect the phosphorylation of p38 mitogen‐activated protein kinase (MAPK) and c‐Jun N‐terminal kinase 1/2. Moreover, blocking the MAPK/ERK1/2 pathway significantly enhanced the anti‐migration ability of geraniin in oral cancer cells. In conclusion, we demonstrated that geraniin inhibits the motility of SCC‐9 and SCC‐14 cells in vitro through a molecular mechanism that involves the attenuation of MMP‐2 expression and activity mediated by decreased FAK/Src and ERK1/2 pathways. 相似文献