大学生社交焦虑与班集体人际关系的调查研究

目的了解大学生社交焦虑和班集体人际关系现状,为高校心理健康保健提供确实的依据。方法对重庆市3所大学共5个班进行交往焦虑量表和大学班级集体人际关系问卷调查。结果农村大学生的社交焦虑高于城镇大学生;男生的负向人缘系数高于女生;非独生子女大学生的负向人缘系数高于独生子女大学生;大学生社交焦虑与负向人缘系数呈显著负相关,大学生的自我认知和自我体验与实际的人际关系并不完全一致。结论大学生社交焦虑与负向人缘系数呈显著负相关。     

Expression of a synapse-associated membrane protein, P84/SHPS-1, and its ligand, IAP/CD47, in mouse retina

P84 and integrin associated protein (IAP) are heterophilic binding partners that are expressed in the central nervous system in addition to a variety of other tissues. Both molecules are known to be involved in cell signaling in nonneural tissues. In the retina, both molecules are expressed prominently in plexiform layers, suggesting a possible association with synapses. Here, we examined the cellular expression and ultrastructural localization of the two molecules in the developing mouse retina. Both appeared to be expressed at one or both sides of synaptic sites, although the expression of IAP in the retina precedes that of P84. Examination of transgenic IAP-null retinae revealed a failure of P84 to become associated with synaptic sites, suggesting the interaction of P84 with IAP was necessary for P84's synaptic localization. These findings suggest that the signaling activities of P84 and IAP are localized to sites of synaptic contact in the retina. Thus this pair of synapse-associated molecules represents a bidirectional signaling system that could function to modify synaptic activity or possibly trophic interactions between central neurons.     

Effect of chronic alcohol consumption on plasma lipid, vitamins A, and E in Korean alcoholics

This human study was conducted to evaluate the effect of chronic alcohol consumption on plasma concentrations of lipid and the antioxidative system in 44 Korean alcoholics and 45 age-, sex-, and nationality-matched nonalcoholic subjects. Plasma triacylglycerols and atherogenic index were higher in alcoholics than in control subjects. Plasma total cholesterol was not different among groups, but plasma high-density lipoprotein cholesterol was lower in alcoholics. There were positive correlations between ethanol consumption and plasma lipid peroxide and atherogenic index in all subjects; there were negative correlations between ethanol consumption and plasma high-density lipoprotein cholesterol in all subjects. There were no significant differences between alcoholics and control subjects in plasma concentrations of α-tocopherol, although plasma α-tocopherol/lipid tended to be lower in alcoholics. Plasma retinol was lower in alcoholics. These results suggest that chronic ethanol consumption can contribute to increased risk for vascular diseases in Korean alcoholics.     

小儿高脂血症危险因素的临床抽样调查

目的通过临床抽样调查，探讨与小儿高脂血症发病有关的危险因素，为小儿高脂血症的早期检测提供客观指标。 方法北京大学第一医院儿科等于2004年9月，调查并分析了962名北京市景山学校在校学生(5~19岁)的血脂浓度，其中采用酶法测定总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL C)及低密度脂蛋白(LDL C)等4项指标。采用坐高、腰围、上臂围、体重指数来反映身体肥胖程度，进一步分析了这些指标与血脂浓度的关系。 结果在962名学生中，共检出高脂血症患儿122例，总检出率为12.7%。高脂血症组的坐高、腰围、上臂围、体重指数4项指标明显高于正常组，2组间差异有显著性(P<0.05)；坐高、腰围、上臂围、体重指数等4项指标与甘油三酯均呈高度显著正相关(P<0.01)，与高密度脂蛋白均呈高度显著负相关(P<0.01)。 结论正常组与高脂血症组的坐高、腰围、上臂围、体重指数4项指标差异均有显著性；坐高、腰围、上臂围、体重指数均是影响甘油三酯和高密度脂蛋白浓度的危险因素。     

北京地区7~18岁青少年血脂现状调查

目的通过最新一轮的北京地区儿童青少年血脂调查，建立新的正常参考值。 方法于2004-09，随机选取7~18岁北京市中小学生共971人，均来自北京市区及郊区各县。根据年龄(每3岁为一年龄段)及性别分为8组(7~9岁男、女组；~12岁男、女组；~15岁男、女组；~18岁男、女组)，应用日立7060型全自动生化分析仪检测其空腹血浆总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL C)及低密度脂蛋白胆固醇(LDL C)浓度，分别取TC、LDL C第75百分位及第90百分位点作为临界高限及高胆固醇血症浓度，取TG第90百分位点作为高甘油三酯血症浓度，取HDL C第5百分位点作为低高密度脂蛋白浓度，建立北京地区儿童血脂参考值。 结果971名儿童的血浆脂蛋白含量值呈正偏态分布，7~9岁男女性别组间血脂4项浓度；~12岁性别组间TG含量女高于男，余3项无统计学差异；~15岁性别组间TC及LDL C含量女高于男，余2项无统计学差异；~18岁性别组间TC及HDL C女高于男，余2项无统计学差异。 结论通过对971名北京市中小学生的血脂调查，建立了北京地区不同年龄性别儿童青少年的血脂正常值，确立了临界高胆固醇和高胆固醇血症浓度、高甘油三酯血症浓度和低高密度脂蛋白浓度。     

Tissue microarray: an effective high-throughput method to study the placenta for clinical and research purposes.

OBJECTIVE: Tissue microarray (TMA) technology allows simultaneous examination of the expression of many molecular markers (protein, mRNA, DNA, etc.) with high-throughput. The application of this technology, to date, has been largely confined to the study of cancer. Placental pathology poses unique challenges because of the size of the organ, its complex anatomy, as well as its histological heterogeneity. The objective of this study was to assess the feasibility and efficiency of TMAs for immunohistochemistry and in situ hybridization of placental tissues. STUDY DESIGN: TMAs were constructed using an automated tissue arrayer. Standard 0.6-mm or 1-mm microarray needles were used. Villous parenchyma, basal plate, and chorioamniotic membranes were targeted in each block. Five mum-thick TMA sections underwent immunohistochemical analysis of both cytoplasmic and nuclear antigens using a panel of antibodies against a variety of cytoplasmic [cytokeratin-7, vascular endothelial growth factor (VEGF), and protein Z], membranous (endoglin), and nuclear (c-fos and c-jun) antigens. mRNA in situ hybridization for surfactant protein A (SP-A) and chromogenic in situ hybridization for the Y chromosome (DYZ1) were also performed. RESULTS: Validation of TMA immunoreactivity demonstrated comparable results with corresponding whole sections. When a two-tiered scoring system (positive/negative) was employed, there was agreement between two and three cores and whole tissue sections (kappa>0.7). When a three-tiered scoring system (negative, weak-positive, or strong-positive) was used, the data from three cores showed the highest agreement with whole tissue sections (kappa >0.7). In situ hybridization experiments for mRNA and DNA were also successful in that the signals were readily detectable. Successful transfer from the donor block to the recipient block differed according to the anatomical compartment. The transfer efficiency of villous parenchyma, basal plate, and chorioamniotic membranes were 96.9% (875/903), 76.7% (115/150), and 75.4% (224/297), respectively. CONCLUSION: TMA is a practical and effective tool for high-throughput molecular analysis of the human placenta. Duplicate and triplicate cores offer agreement with whole tissue sections for two-category distinction immunostaining. TMA also affords relevant results from in situ hybridization experiments for mRNA and DNA. The major advantages are the conservation of tissues and reagents, simultaneous comparison of molecular markers in different anatomical compartments of the placenta, and reduction of experimental error.     

Relationsip between PTEN and VEGF Expression and Clinicopathological Characteristics in HCC

Recent reports have identified that phosphatase and tensin homlog deleted on chromosome ten protein (PTEN) is a tumor suppressor gene in the human body with a double-specific phosphatase activity of lipid phosphatase and protein phosphatase[1]. Vascular endo-thelial growth factor (VEGF) is known as one of the ut-most factors stimulating tumor blood vessel growth. Hepatocellular carcinoma (HCC) is a solid malignant tumor, whose growth is rapid, with frequent intrahepatic dissemination and m…     

婴幼儿骶尾部良性畸胎瘤的MRI诊断

目的：评估婴幼儿骶尾部良性畸胎瘤的MRI诊断价值。方法：回顾性分析6例经手术病理证实的骶尾部良性畸胎瘤的MRI表现。6例均用MRI的快速自旋回波序列扫描，结合短时反转恢复脂肪抑制序列及增强扫描。结果：骶尾部良性畸胎瘤起源于盆腔内末端骶尾区，属于后腹膜肿瘤，MRI通常表现为圆形、类圆形或不规则形巨大均质或非均质性肿块，边界一般较清楚，信号混杂，其中有代表囊性或实性成分的信号区域，实性部分常见脂肪或钙化。结论：MRI可清楚显示盆腔内、外的成分和解剖细节，有助于对骶尾部良性畸胎瘤的诊断以及合适手术计划的选择。     

RP-HPLC制备色谱法分离淡豆豉中高纯度异黄酮苷元

淡豆豉经大孔树脂柱色谱初步分离纯化,采用RP-HPLC制备色谱法,乙腈-水-冰醋酸(35∶ 65∶ 1,v/v/v)为流动相,流速3.0 ml/min,检测波长260 nm.结果表明,在30 min内快速分离到大豆素、大豆黄素和染料木素,产物纯度均达到99%以上.     

Risk Factors of Gastric Cancer in Wuwei City- an Endemic Region of Gastric Cancer

OBJECTIVE Studies have shown that risk factors of gastric cancer include living habits, eating moldy pickled food, dystrophy, lack of nutrients, and genetics, etc. This study explores the prevalence of these factors in Wuwei, a city in northwest China with a high incidence of gastric cancer.METHODS Family histories of the residents in Wuwei City were investigated with a case-control method. Segregation ratio and heredity degree of gastric cancer were calculated using theLi-Mantel-Cart and Falconer's regression methods. Chronic gastritis patients were followed-up by home visits, gastroscopy, and pathology. Carcinogenic fungi and volatilile N-nitroso compounds were noted in the residents' meals. Helicobactor pylori (Hp) was assessed in their gastric mucosa and the total content of serum vitamin C in 293 healthy adults was determined.RESULTS In Wuwei City, the segregation ratio of gastric cancer was 0.077. The heredity degree of first-degree relatives was 22.91%. The incidence rate of cancer per year in people in an atrophic gastritis group was 1.09%. Eight kinds of nitrosamines and 14 kinds of carcinogenic fungi were detected in the residents' food. Total average vitamin C in the serum of the 293 healthy adults in the summer was 5.74±2.79 mg/L. The positive rate of Hp in gastric mucosa of the residents was 67%.CONCLUSION The majorextrinsic factors include infection of Hp, atrophic gastritis (especially atrophic hyperplasia), and genetic susceptibility.