老年大鼠松质骨骨重建的组织形态计量学研究

目的：研究老年大鼠及在促骨合成药前列腺素E2（PGE2）作用下松质骨骨重建和骨建造的形态计量学改变，探讨动物骨重建形态学新参数测量方法及其意义。方法：50只20月龄雄性Wistar大鼠随机分成5组，年龄对照组（基础组、10d和30d年龄对照组），PGE2给药组（分别10d和30d给予3mg/kg/d处理组）。用体内双荧光标记，不脱钙组织切片，粘合线（cement line）染色，骨组织形态计量学方法，测定骨重建和骨建造参数。结果：20月龄雄性大鼠胫骨近端松质骨的形成表面大多数为骨重建单位（占63．3％），少部分为骨建造单位（占26．7％）；PGE2用药后骨重建单位增加1．5倍，骨建造单位增加4倍，比值倒置，成骨细胞10d时明显增多。说明PGE2通过刺激成骨细胞骨合成而介民导骨建造性骨增加和骨重建性骨量增加，并以前为主。结论：老年雄性大鼠 松质骨以骨重建活动为主，仍有骨建造活动。PGE2主要通过刺激成骨细胞骨建造而增加骨量。     

Cataract surgery rates in Ontario, Canada, from 1992 to 2004: more surgeries with fewer ophthalmologists

BACKGROUND: To evaluate trends in cataract surgeries in Ontario between 1992 and 2004. METHODS: A retrospective analysis of the number of cataract surgeries performed in Ontario from April 1992 to March 2005. The estimated prevalence of cataract and cataract surgeries per 1000 persons at risk was calculated. RESULTS: The number of cataract surgeries in Ontario increased from 44,943 in 1992 to 109,506 in 2004 (143.6%, 12.08% annual increase). The number of cataract surgeries per 1000 patients at risk of cataract increased from 64.6 in 1992 to 115.65 in 2004 (79%, 4.97% increase per year). This rate was strongly positively correlated with time and with the increase in the Ontario population (r = 0.920 and r = 0.922, respectively; p < 0.001). The number of ophthalmologists increased by 5.3% from 1992 to 1997 and then decreased by 2.9% by 2004. This change was not correlated with the cataract surgery rates (r = 0.475; p = 0.10). However, the number of ophthalmologists per million population decreased by 13.4% between 1992 and 2004. This number had a statistically negative correlation with cataract surgery rates (r = -0.757; p < 0.01). INTERPRETATION: There has been a significant increase in the number of cataract surgeries in Ontario despite a decrease in the number of ophthalmologists per million population.     

Effectiveness of Half-Cut Wood Training of Close and Kinetic Chain Method on Mental Health and Physical Health of Patients with Knee Instability in China

Knee instability as a symptom of ligament injury usually only receives attention when it causes pain or impacts patients’ mobility in China. In this study both the physical and psychosocial impact of an innovative approach to treatment, Half-cut Wood Training, was examined. Twenty individuals with knee instability who received Halfcut Wood Training (Intervention group) and twenty two individuals with knee instability who did not receive Half-cut Wood Training (as Control group) participated in the study. The electric WIFI based HBF-306 was used to collect the anthropometry and biomedical data. Symptom severity was assessed by a doctor and through response to a specific question. Half-cut Wood Training was used as the intervention for the knee instability patients, and the effects among pre-intervention, post-intervention and control group on self-esteem, self-efficacy, quality of life and reductions in knee instability were assessed and compared to patients in the control group. The Half-cut Wood Training has significantly improved the physical health, self-esteem, self-efficacy, legs and feet functioning of patients in the intervention group compared with control group. Moreover, the Symptom were decreased (F = 15.47, P < 0.001), and the doctor reported knee function was improved (F = 36.20, P < 0.001). The subgroups including number of sessions of attending the Half-cut Wood Training had effects on the reduction of symptoms and duration of training time more than one month has effect on the improvement of physical health. Half-cut Wood Training provides beneficial means of improving knee function, self-esteem, self-efficacy, and physical health of the quality of life of patients with the knee instability.     

Exotic and indigenous problem plants species used,by the Bapedi,to treat sexually transmitted infections in Limpopo Province,South Africa

Background

The ethnic usage of exotics and indigenous problem plants is a highly debated topic, as legislative requirements over-shadow their potential medicinal value, particularly to treat sexually transmitted infections (STIs). Limited information exists regarding their medicinal value among the Bapedi.

Objectives

To ascertain the importance of exotics and indigenous problem plants in the treatment of STIs, a major global primary health care challenge.

Methods

A field observation and semi-structured questionnaire focussing on species diversity, types of STIs treated and medicinal preparation as well as application was used to collect data from 34 traditional healers.

Results

Seven exotics and three indigenous problem species were identified. These species were used to treat four STIs; with Catharanthus roseus illustrating its dominance in the treatment of gonorrhoea. Some medicinal species used by Bapedi traditional healers have been validated through scientific research or through their extensive use by various cultures in South Africa and other parts of Africa. To the best of our knowledge Alternanthera pungens, Caesalpinia decapetala, Cinnamomum verum, and Citrullus lanatus are reported for the first time in the treatment of the investigated STIs.

Conclusions

Exotic and indigenous problem species constitute an important component of the STIs treatment protocol. Their utilization by Bapedi cautions against the narrow-minded approach of indiscriminate eradication, as these species can play a significant role in the primary health care needs of socio-economic vulnerable people.     

耐一线药物及注射药物对耐多药结核病治疗效果的影响

背景和目的:最近的研究结果表明,对其他一线药物和注射类药物(如卡那霉素、卷曲霉素)等耐药是影响耐多药结核病(MDR-TB)患者治疗效果的独立危险因素.本研究旨在明确耐其他一线药物和注射类药物对韩国不合并人免疫缺陷病毒(HIV)感染的MDR-TB患者临床疗效的影响.方法:采用回顾性队列研究分析1996年1月至2005年12月首尔国家大学附属医院治疗的211例MDR-TB患者治疗效果,排除7例丢失和7例迁出,对197例患者进行了最终分析.     

Application of a comprehensive subtelomere array in clinical diagnosis of mental retardation

In 2-8% of patients with mental retardation, small copy number changes in the subtelomeric region are thought to be the underlying cause. As detection of these genomic rearrangements is labour intensive using FISH, we constructed and validated a high-density BAC/PAC array covering the first 5 Mb of all subtelomeric regions and applied it in our routine screening of patients with idiopathic mental retardation for submicroscopic telomeric rearrangements. The present study shows the efficiency of this comprehensive subtelomere array in detecting terminal deletions and duplications but also small interstitial subtelomeric rearrangements, starting from small amounts of DNA. With our array, the size of the affected segments, at least those smaller than 5 Mb, can be determined simultaneously in the same experiment. In the first 100 patient samples analysed in our diagnostic practice by the use of this comprehensive telomere array, we found three patients with deletions in 3p, 10q and 15q, respectively, four patients with duplications in 9p, 12p, 21q and Xp, respectively, and one patient with a del 6q/dup 16q. The patients with del 3p and 10q and dup 12p had interstitial rearrangements that would have been missed with techniques using one probe per subtelomeric region chosen close to the telomere.