The association between caudal block and urethroplasty complications of distal tubularized incised plate repair: experience from a South China National Children’s Medical Center

BackgroundThe effect of caudal block (CB) on the incidence of urethroplasty complications in hypospadias repair remains controversial. The evidence is conflicting, and some confounding bias issues need to be addressed. We sought to study a more homogenous group of distal hypospadias patients undergoing primary tubularized incised plate (TIP) repair by a senior pediatric urology surgeon in the past 2 years to examine the relationship between urethroplasty complications and the use of CB.MethodsWe reviewed our database to identify consecutive patients who had undergone hypospadias repairs by a senior director surgeon at our Center between January 2018 and November 2020. To be eligible to participate in the study, patients had to meet the following inclusion criteria: (I) have distal hypospadias; (II) have undergone a primary TIP repair; and (III) have attended follow-up appointments for a minimum period of 6 months. The primary outcome was the development of urethroplasty complications during the follow-up period. The principal variable of interest was whether or not CB was used perioperatively. The patients were categorized into a CB group (general anesthesia combined with CB) or a control group (general anesthesia only). Other potential risk factors were analyzed, including patient age at operation, patient weight, glans width, and the length of the urethral plate defect.ResultsThirty (12.2%) of the distal patients developed postoperative surgical complications. The postoperative surgical complication rates were similar between the different anesthesia groups. Weight, the length of the urethral plate length, and glans width did not contribute to the risk. Age was the only independent risk factor for postoperative surgical complications, and the complication rates increased in older patients.ConclusionsOur data from consecutive TIP repairs in distal hypospadias patients indicated no association between the use of CB anesthesia and the postoperative urethroplasty complication rate. Patients who were older in age when they underwent surgery had a higher risk of complications.     

Assessment of Carbendazim Residues and Safety in Celery Under Different Cultivation Conditions

Although the carbendazim is widely used to manage spot blight in celery cultivation, information on residues identified is of interest. In this study, we examined the dissipation and residual amounts of carbendazim in celery and soil under different cultivation methods when using the suggested dose and ten times of that and the bioconcentration factor of carbendazim for celery. The results showed that when celery leaves were sprayed with the suggested dose, the half-lives in a celery field and greenhouse were 2.75 days and 3.29 days, respectively. When the soil matrix was sprayed with the recommended dose before cultivation, the half-lives of carbendazim residues were 16.86 days and 11.97 days. We also conducted a long-term dietary risk assessment using the corresponding criteria. The results showed that, in China, the use of carbendazim at a dose of 0.022 g/m² is safer and more reasonable when the harvest interval is 28 days.

     

建立人微小病毒B19的PCR检测方法

人微小病毒Ｂ１９是微小病毒科中唯一能感染人的病毒。能够提供Ｂ１９抗原的病毒体外培养系统尚未建成，因此限制了血清学实验的开发和普及。为此作者建立起Ｂ１９病毒的ＰＣＲ检测方法。设计引物在表达外壳蛋白ＶＰ１基因区，扩增长度为４００ｂｐ。     

单片微机化微弱发光测量仪及其在肿瘤研究中的初步应用

单片微机化微弱发光测量仪由中科院生物物理所和北京科龙生物医学技术开发公司研制成功。该仪器适用于肿瘤研究中生物医学样品的微弱发光的测量。测量样品的重复性优于０．８％，稳定性优于０．５％，线性相关系数达０．９９９７，不仅可以进行样品的发光强度测量，还可以进行动力学曲线的测量以及样品发射光谱的测量。光谱在４００ｎｍ－７５０ｎｍ范围内。测量方式可选择手动、半自动和自动计时三种。测量结果表明，荷瘤裸鼠血液微     

呋喃唑酮厌恶疗法戒酒的初步报告

对8例酒精依赖伴中毒男性患者,采用呋喃唑酮结合小量饮酒方法进行临床观察,因其产生不良躯体反应而达到厌恶戒酒目的。其成功率为87.5%。远期不良反应轻微。戒酒机制可能属厌恶条件反射的建立。     

心电心向量同步仪的研制

本文介绍一种以３８６计算机为核心的心电心向量同步仪。阐述了仪器的工作原理、硬件组成、软件设计。     

Genes of Hippo signaling network act unconventionally in the control of germline proliferation in Drosophila.

Hippo pathway and its related genes are required for growth control in various somatic tissues. The mutations of Hippo pathway components lead to tissue overgrowth cell-autonomously. Surprisingly, when we generated germline mutant clones of Hippo-network genes such as fat, expanded, hippo, salvador, and warts, we did not observe any overgrowth of these mutant cells. Consistently, overexpression of the progrowth gene yorkie, which is normally inhibited by Hippo signaling, did not lead to germline overgrowth either. In contrast to previous studies in epithelial tissues, these tumor suppressor genes are dispensable in germline cells for their proliferation control. Furthermore, we demonstrate that expanded functions nonautonomously to regulate spermatogonial proliferation. It appears that expanded acts from the somatic support cells surrounding the germline to restrict spermatogonial amplification.     

Human monocyte-derived hemangioma-like endothelial cells: evidence from an in vitro study

BACKGROUNDS: Hemangiomas are highly prevalent in newborns and infants and can lead to severe complications. However, the pathogenesis of hemangiomas is still unknown. This study was designed to examine the potential of human monocytes to differentiate into hemangioma endothelial cells. METHODS: Purified monocytes from adult human peripheral blood were cultured under a conditional culture environment supplemented with basic fibroblast growth factor and vascular endothelial growth factor. Cells cultured for 2 weeks were subjected to histological and immunochemical examinations in order to determine the expression of specific markers for hemangioma endothelial cells. RESULTS: Monocytes cultured for 2 weeks in angiogenic medium expressed human erythrocyte-type glucose transporter protein, FcgammaRII, and several other endothelial markers, all of which are deemed specific markers for hemangioma endothelial cells. However, neither CD133 nor alpha smooth muscle actin was detected in our monocyte culture. CONCLUSION: Our data suggested that monocytes are capable of differentiating into hemangioma endothelial cells under the angiogenic stimulation from microenvironment of proliferative hemangioma.     

Exact BPF and FBP algorithms for nonstandard saddle curves

A hot topic in cone-beam CT research is exact cone-beam reconstruction from a general scanning trajectory. Particularly, a nonstandard saddle curve attracts attention, as this construct allows the continuous periodic scanning of a volume-of-interest (VOI). Here we evaluate two algorithms for reconstruction from data collected along a nonstandard saddle curve, which are in the filtered backprojection (FBP) and backprojection filtration (BPF) formats, respectively. Both the algorithms are implemented in a chord-based coordinate system. Then, a rebinning procedure is utilized to transform the reconstructed results into the natural coordinate system. The simulation results demonstrate that the FBP algorithm produces better image quality than the BPF algorithm, while both the algorithms exhibit similar noise characteristics.