全文获取类型
收费全文 | 82613篇 |
免费 | 7428篇 |
国内免费 | 4561篇 |
专业分类
耳鼻咽喉 | 712篇 |
儿科学 | 1173篇 |
妇产科学 | 869篇 |
基础医学 | 9191篇 |
口腔科学 | 2330篇 |
临床医学 | 8968篇 |
内科学 | 11386篇 |
皮肤病学 | 1253篇 |
神经病学 | 4596篇 |
特种医学 | 3659篇 |
外国民族医学 | 22篇 |
外科学 | 10549篇 |
综合类 | 14196篇 |
现状与发展 | 13篇 |
一般理论 | 16篇 |
预防医学 | 6179篇 |
眼科学 | 1608篇 |
药学 | 8210篇 |
52篇 | |
中国医学 | 3968篇 |
肿瘤学 | 5652篇 |
出版年
2024年 | 167篇 |
2023年 | 700篇 |
2022年 | 1529篇 |
2021年 | 2693篇 |
2020年 | 2157篇 |
2019年 | 1732篇 |
2018年 | 2156篇 |
2017年 | 2065篇 |
2016年 | 1814篇 |
2015年 | 2910篇 |
2014年 | 3871篇 |
2013年 | 4326篇 |
2012年 | 6092篇 |
2011年 | 6443篇 |
2010年 | 4992篇 |
2009年 | 4406篇 |
2008年 | 4956篇 |
2007年 | 4725篇 |
2006年 | 4533篇 |
2005年 | 3770篇 |
2004年 | 2826篇 |
2003年 | 2419篇 |
2002年 | 1966篇 |
2001年 | 3156篇 |
2000年 | 2932篇 |
1999年 | 2351篇 |
1998年 | 977篇 |
1997年 | 745篇 |
1996年 | 500篇 |
1995年 | 436篇 |
1994年 | 399篇 |
1993年 | 308篇 |
1992年 | 1068篇 |
1991年 | 951篇 |
1990年 | 835篇 |
1989年 | 915篇 |
1988年 | 830篇 |
1987年 | 760篇 |
1986年 | 711篇 |
1985年 | 613篇 |
1984年 | 388篇 |
1983年 | 320篇 |
1982年 | 183篇 |
1981年 | 140篇 |
1980年 | 119篇 |
1979年 | 204篇 |
1974年 | 84篇 |
1973年 | 97篇 |
1972年 | 95篇 |
1971年 | 94篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
61.
螺旋CT双期增强扫描与病灶延迟期扫描在肝癌诊断中的应用 总被引:1,自引:0,他引:1
目的:研究螺旋CT动脉期、门脉期及病灶延迟期扫描在肝癌诊断中的应用,以进一步选择理想的扫描时期和方案。方法:对48例肝细胞性肝癌患者行平扫及增强扫描,造影剂注射速率为3ml/s,然后开始动脉期、门脉期及病灶延迟期扫描。统计各期的病灶检出数,观察病灶在不同时期的特征。结果:动脉期、门脉期及病灶延迟期的病灶检出率分别为91.9%、76.9%、78.8%,动脉期和门脉期、病灶延迟期之间均有显著差异,而门脉期和病灶延迟期之间无差异。结论:在肝癌的检出方面动脉期价值最大,而门脉期和延迟期之间无差异。双期扫描(动脉期加门脉期)可作为肝癌CT诊断的常规技术,对不典型病例,病灶延迟期很有必要,有助于病灶的定性。 相似文献
62.
Ames试验检测三硝基甲苯及其还原代谢产物的诱变性 总被引:1,自引:1,他引:0
应用Ames试验检测系统观察了三硝基甲苯及其还原代谢产物4-羟氨基-2,6-二硝基甲苯(4-HA),4-氨基-2,6-二硝基甲苯(4-A),2-氨基-4,6-二硝基甲苯(2-A)和2,4-二氨基-6-硝基甲苯(2,4-DA)的诱变活性,实验结果显示,三硝基甲苯及其代谢产物具有明显的致突变作用,4-A的直接诱变活性最强,而且移码型诱变性更为显著,在微粒体酶系统的参与下,TNT的诱变活性增强,其代谢产 相似文献
63.
This study examined the immunoregulatory role of recombinant interleukin 4 (IL-4), also known as B-cell stimulating factor 1, on the generation of cytotoxic effector cells from normal and leukaemic human blood mononuclear cells. When tested on cells from normal individuals, the addition of IL-4 to mixed lymphocyte cultures led to a dose-dependent proliferation of T-helper cells (CD3, 4 positive) with a concomitant decrease in phenotypic and functional cytotoxic T cells and natural killer (NK) cells. IL-4 also inhibited the interleukin-2 (IL-2)-induced generation of lymphokine-activated killer (LAK) activity when added at the beginning of mixed lymphocyte culture. When tested on mature leukaemic NK cells, IL-4 also inhibited the ability of IL-2 to induce LAK function using a short-term culture system. These results show that IL-4 acts on both normal and leukaemic cells and suggests that it acts at more than one level during the development of LAK function. 相似文献
64.
U. Ravens Michael O. Flüß Q. Li Herbert M. Himmel Erich Wettwer Michael Klockow Inge Lues 《Naunyn-Schmiedeberg's archives of pharmacology》1997,355(6):733-742
The thiadiazinone derivative [+]-EMD 60263 ((+)-5-(1-(α-ethylimino-3,4-dimethoxybenzyl)-1,2,3,4- tetrahydroquinoline-6-yl)-6-methyl-3,6-dihydro-2H-1,3,4
-thiadiazine-2-on) is a Ca2+-sensitizing agent with only minor phosphodiesterase inhibitory activity. Our aim was to characterize the inotropic and electrophysiological
effects of [+]-EMD 60263 and its enantiomer [-]-EMD 60264 in several cardiac muscle preparations. The Ca2+-sensitizing activity resided in the [+]-enantiomer only. [+]-EMD 60263 (3 μM) shifted the EC50 of Ca2+ for contractile activation of skinned fibers of pig heart from 2.41 μM to 0.73 μM, whereas [-]-EMD 60264 (30 μM) was ineffective.
In Langendorff-perfused guinea pig hearts, [+]-EMD 60263 and [-]-EMD 60264 induced concentration-dependent positive and negative
inotropic effects, respectively; both enantiomers reduced spontaneous heart rate but did not influence perfusion pressure.
The maximum increase in force of human atrial trabeculae was 35 % of pre-drug control with [+]-EMD 60263 in comparison to
113 % with forskolin. In guinea-pig papillary muscles, [+]-EMD 60263 and [-]-EMD 60264 had opposite inotropic responses, however,
both agents similarly prolonged action potential duration. Both enantiomers concentration-dependently blocked the rapidly
activating component IKr of the delayed rectifier in guinea-pig myocytes. The block saturated at potentials positive to +30 mV, closely resembling
the effects of the antiarrhythmic agent E-4031 which had been originally used to define IKr. It is concluded, that the positive inotropic action of [+]-EMD 60263 can be explained by prevalence of the Ca2+-sensitizing effect. The accompanying prolongation in action potential duration is caused by block of the IKr component of the delayed rectifier. While the inotropic effects are stereoselective, most of the electrophysiological actions
are clearly independent of sterical configuration. The combination of Ca2+-sensitizing with class-III antiarrhythmic action may provide an interesting pharmacological profile of potential therapeutic
use.
Received: 7 January 1997 / Accepted: 25 February 1997 相似文献
65.
本文采用PCR技术对石家庄地区216名无关个体DIS80(pMCT118)位点扩增片段长度多态性的等位基因频率进行调查。PCR扩增产物采用聚丙烯酰胺凝胶电泳分型及溴化乙锭染色,共检出70种基因型,25个等位基因,其中以18、24、30三个等位基因频率最高。将本文结果与日本人群在DIS80(pMCT118)位点多态性结果进行比较,石家庄地区汉族人群DIS80(pMCT118)位点基因频率分布符合HardyWeinberg平衡定律,该位点非父排除率为0.71,个体识别率为0.9,杂合度为0.85。 相似文献
66.
慢性低氧大鼠肺动脉内皮素mRNA的表达及定位 总被引:1,自引:0,他引:1
目的:探讨慢性低氧大鼠肺动脉内皮素-1基因表达及分布。方法:采用生物素标记cRNA探针,对Wistar大鼠肺动脉进行原位杂交。结果:低氧1周组及低氧2周组大鼠大部分肺动脉内皮细胞ET-1mRNA表达呈阳性信号,而对照组大鼠仅极少数阳性信号(P<0.01);低氧1周组大鼠部分显示强阳性信号。低氧1周组及低氧2周组大鼠部分肺动脉平滑肌细胞呈现ET-1mRNA表达阳性信号,而对照组大鼠则无阳性信号表达(P<0.01及0.05)。结论:慢性低氧对大鼠肺动脉ET-1分泌的影响是在基因转录水平进行,且主要细胞定位在肺动脉内皮细胞,此外还包括肺动脉平滑肌细胞。 相似文献
67.
Investigation of Role of Nitric Oxide in Protection from Bordetella pertussis Respiratory Challenge
下载免费PDF全文
![点击此处可从《Infection and immunity》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The mechanism whereby whole-cell pertussis vaccines (WCV) confer protection against Bordetella pertussis is still not fully understood. We have previously reported that macrophage activation produced by vaccination with WCV is associated with induction of NO synthesis by macrophages in response to in vitro stimulation with B. pertussis antigens. To determine whether NO production is an effector of protection or simply a marker of activation, the susceptibility of inducible nitric oxide synthase (type II, iNOS) knockout mice to infection with B. pertussis was examined. We showed that iNOS knockout mice were more susceptible to B. pertussis respiratory challenge than wild-type mice. iNOS-deficient mice also developed a less effective protective response than wild-type mice after the same immunization with WCV. This suggests that NO plays an important role in effecting protection against B. pertussis challenge. 相似文献
68.
C W Tabor H Tabor Q W Xie 《Proceedings of the National Academy of Sciences of the United States of America》1986,83(16):6040-6044
We have obtained Escherichia coli mutants lacking spermidine synthase (putrescine aminopropyltransferase) and have found that the mutated gene (speE) is located immediately upstream from the gene coding for S-adenosylmethionine decarboxylase (speD); these genes are located at 2.7 minutes on the E. coli chromosome. Both genes are present in a 1795-base-pair fragment of E. coli DNA that was cloned into pBR322. Deletion of 105 bases upstream of speE caused a coordinate loss of both activities, indicating that speE and speD constitute a single operon. speE and speD have also been cloned separately in a high-expression vector; strains carrying these plasmids overproduce the respective enzymes. 相似文献
69.
70.
The possible reversal by calcium of the inhibitory action of verapamil on the atrioventricular (AV) node was investigated in anesthetized, atropinized dogs, with cardiac pacing. The His bundle potentials were recorded by endocavitory electrode and the AV node effective refractory period measured by the extrastimulus method. Calcium infusion was effective against the impairment of AV nodal conduction induced by verapamil, provided it remained moderate: the gradual rise in the plasma calcium concentration counteracted the effects of an infusion of verapamil on conduction time and effective refractory period in the AV node, as long as it did not exceed 5 mmol/L. However, beyond this level, calcium appeared less and less capable of reversing the effects of verapamil. Thus, the protective action of calcium had a bell-shaped dose-response curve, with the optimum at 5 mmol/L. This biphasic influence is consistent with the opposite opinions previously given concerning the antagonism between calcium and calcium blockers, depending on whether hypercalcemia brought into play was mild or major. In any case, the prominent role played by calcium in the slow inward current in the AV node accounts for the antagonism, observed in vivo, between calcium and verapamil. The pacemaker activity of the sinoatrial (SA) node was less influenced by both calcium blocker and calcium. 相似文献