大孔树脂分离纯化紫苏总黄酮的工艺研究

目的:筛选分离纯化紫苏总黄酮的最佳树脂,并确定其工艺条件。方法:以总黄酮吸附量和回收率为考察指标,采用静态吸附分离法确定适合的大孔吸附树脂;采用动态吸附分离法确定分离条件。结果:AB-8树脂对紫苏总黄酮有良好的吸附分离性能。其动态分离纯化工艺条件为:紫苏总黄酮上样液浓度为1.5mg/mL,最大吸附量为11.5mg/g,吸附速度为1.0mL/m in,95%乙醇2倍柱体积1.0mL/m in洗脱,树脂可重复使用3次。结论:该方法操作简便、有效,适于紫苏总黄酮的吸附纯化。     

大孔树脂分离纯化紫苏总黄酮的工艺研究

目的：筛选分离纯化紫苏总黄酮的最佳树脂,并确定其工艺条件。方法：以总黄酮吸附量和回收率为考察指标,采用静态吸附分离法确定适合的大孔吸附树脂;采用动态吸附分离法确定分离条件。结果：AB-8树脂对紫苏总黄酮有良好的吸附分离性能。其动态分离纯化工艺条件为：紫苏总黄酮上样液浓度为1.5mg/mL,最大吸附量为11.5mg/g,吸附速度为1.0mL/m in,95%乙醇2倍柱体积1.0mL/m in洗脱,树脂可重复使用3次。结论：该方法操作简便、有效,适于紫苏总黄酮的吸附纯化。     

南京扬子石化公司10701名职工体检结果分析

目的为了解南京扬子石化公司职工的健康状况，以做好职工的预防保健工作。方法对该公司10701名职工进行健康检查，记录统计结果，按性别、年龄等进行归类分析。结果10701名职工中，发现各类疾病（至少有1种以上症状、体征或辅助检查异常者）9927人，占体检总人数的92．8％。其中高脂血症、肥胖症、脂肪肝、高血压等慢性非传染性疾病位于患病率的前列。结论预防慢性病的发生是预防保健工作的重点，教育职工养成健康生活方式和改变不良的习惯行为是防治慢性病的关键。     

Risk factors in enterococci isolated from foods in Morocco: Determination of antimicrobial resistance and incidence of virulence traits

A collection of enterococci isolated from meat, dairy and vegetable foods from Morocco including 23 Enterococus faecalis and 15 Enterococcus faecium isolates was studied. All isolates were sensitive to ampicillin, penicillin, and gentamicin. Many E. faecalis isolates were resistant to tetracycline (86.95%), followed by rifampicin (78.26% ciprofloxacin (60.87%), quinupristin/dalfopristin (56.52%), nitrofurantoin (43.47%), levofloxacin (39.13%), erythromycin (21.73%), streptomycin (17.39%), chloramphenicol (8.69%), vancomycin (8.69%), and teicoplanin (4.34%). E. faecium isolates showed a different antibiotic resistance profile: a high percentage were resistant to nitrofurantoin (73.33%), followed by erythromycin (66.60%), ciprofloxacin (66.66%), levofloxacin (60.00%), and rifampicin (26.66%), and only a very low percentage were resistant to tetracycline (6.66%). One isolate was resistant to vancomycin and teicoplanin. The incidence of virulence factors was much higher among E. faecalis isolates, especially for genes encoding for sex pheromones, collagen adhesin, enterococcal endocarditis antigen, and enterococcal surface protein. Isolates with multiple factors (both antibiotic resistance and virulence traits) were also more frequent among E. faecalis isolates, in which one isolate cumulated up to 15 traits. By contrast, several isolates of E. faecium had only very few unwanted traits as compared to only two isolates in E. faecalis. The high abundance of isolates carrying virulence factors and antibiotic resistance traits suggests that the sanitary quality of foods should be improved in order to decrease the incidence of enterococci.     

儿童补锌与生长发育相关性研究

目的:探讨儿童补锌对血锌水平的影响及与儿童体重、身高、智商等发育的相关关系。方法:用分层加单纯随机抽样的方法对抽取的168例儿童进行体格检查、血锌浓度测定、智商测定,并问卷调查儿童饮食、补锌等情况,采用SPSS10.0版统计软件进行多元线性回归分析。结果:①本组168例儿童血锌平均浓度为58.98μmol/L,正常血锌儿童占75.0%,低血锌儿童占25.0%。②儿童血锌水平与性别无关,与年龄有高度显著性差异(χ2=20.08,P<0.01),3~6岁儿童缺锌比例较7~12岁儿童高。③血锌水平影响儿童体重、身高发育,低血锌儿童体重、身高发育指标低于正常的比例高(χ2=24.52,P<0.01,χ2=29.03,P<0.01,)。④影响血锌水平的因素主要为饮食与补锌情况。⑤影响智商的因素为血锌水平与补锌情况。结论:正常饮食儿童低血锌比例低,偏食、挑食、厌食是引起血锌偏低的主要因素,血锌水平影响智商高低。科学对待儿童缺锌与补锌,理性对待广告宣传,纠正不良饮食习惯是保障儿童锌营养的关键。     

The average cumulative risks of breast and ovarian cancer for carriers of mutations in BRCA1 and BRCA2 attending genetic counseling units in Spain.

PURPOSE: It is not clear that the published estimates of the breast and ovarian cancer penetrances of mutations in BRCA1 and BRCA2 can be used in genetic counseling in countries such as Spain, where the incidence of breast cancer in the general population is considerably lower, the prevalence of BRCA2 mutations seems to be higher, and a distinct spectrum of recurrent mutations exists for both genes. We aimed to estimate these penetrances for women attending genetic counseling units in Spain. EXPERIMENTAL DESIGN: We collected phenotype and genotype data on 155 BRCA1 and 164 BRCA2 mutation carrier families from 12 centers across the country. Average age-specific cumulative risks of breast cancer and ovarian cancer were estimated using a modified segregation analysis method. RESULTS: The estimated average cumulative risk of breast cancer to age 70 years was estimated to be 52% [95% confidence interval (95% CI), 26-69%] for BRCA1 mutation carriers and 47% (95% CI, 29-60%) for BRCA2 mutation carriers. The corresponding estimates for ovarian cancer were 22% (95% CI, 0-40%) and 18% (95% CI, 0-35%), respectively. There was some evidence (two-sided P = 0.09) that 330A>G (R71G) in BRCA1 may have lower breast cancer penetrance. CONCLUSIONS: These results are consistent with those from a recent meta-analysis of practically all previous penetrance studies, suggesting that women with BRCA1 and BRCA2 mutations attending genetic counseling services in Spain have similar risks of breast and ovarian cancer to those published for other Caucasian populations. Carriers should be fully informed of their mutation- and age-specific risks to make appropriate decisions regarding prophylactic interventions such as oophorectomy.     

Tumeur à cellules de la granulosa juvénile de l’ovaire: à propos de deux cas

Juvenile granulosa cell tumor of the ovary is a rare neoplasm that makes up less than 5% of ovarian tumors in childhood and adolescence. The majority of they tumors are diagnosed in stage I (FIGO) with a favorable prognosis. The aim of this work was to describe the pathological, clinical and therapeutic features of this rare tumor of ovary. The authors describe a juvenile cell tumor expressed by an abdominal and genital syndrome occurring in 30 and 35-year-old two young females. The tumor was strictly localized in the ovary in one case; the second patient presented a bilateral ovarian tumor and peritoneal metastasis at the diagnosis. Clinical follow-up of the latter patient showed a recurrence with liver metastasis in spite of a radical surgical treatment followed by chemotherapy. The first patient has a favorable evolution with a follow-up of two years. Various prognostic factors of the juvenile granulosa cell tumor of the ovary are currently being evaluated. Surgery is the treatment for local disease. Patients with locally advanced, recurrent or metastatic tumors require chemotherapy, although the optimal regimen remains to be determined.     

Erythermalgia: a rare vascular acrosyndrome]

A case of erythermalgia with arterial hypertension that appeared in a 13 year-old boy is described. This condition led to a loss of weight of 10 kg within one month. None of the diseases known as a cause of this rare condition was found. Clinical manifestations were only improved when extremities were placed in cold water: treatment with pizotifene was also effective suggesting the role of serotonine in the mechanism of the crises.