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Andreana Marino Simona Pergolizzi Eugenia R. Lauriano Giuseppe Santoro Francesca Spataro Francesco Cimino Antonio Speciale Antonia Nostro Giuseppe Bisignano 《APMIS : acta pathologica, microbiologica, et immunologica Scandinavica》2015,123(2):163-168
The Toll‐Like Receptor 2 (TLR2) plays an active and important role in Staphylococcus aureus‐induced chronic ocular inflammation. The aim of this study was to investigate the expression and function of TLR2 of corneal stromal cells in ex vivo rabbit model of S. aureus keratitis. Corneal buttons with sclera rims placed in an ex vivo air‐interface organ culture were assigned to two groups: corneas with epithelial and stromal abrasions. Each group was then divided into two sub‐groups exposed to UV‐killed S. aureus ATCC 6538P and S. aureus ATCC 29213, respectively. TLR2 and IL‐8 mRNA expressions were analyzed by quantitative real‐time RT‐PCR. TLR2 localization was visualized by immunofluorescence analysis. The results demonstrated that TLR2 and IL‐8 mRNA were significantly expressed in the stromal cells of the groups exposed to S. aureus strains. Moreover, it has been demonstrated that, after corneal injury, keratocytes differentiated into myofibroblasts became able to express TLR2 only when exposed to S. aureus. Identification of mechanisms regulation of corneal TLRs may lead to development of therapeutic interventions aimed at controlling corneal inflammation. This ex vivo model can be used to clarify the molecular events of bacterial‐corneal tissue interactions and their inflammatory consequences. 相似文献
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Antonia‐Leda Matalas Charles E. Franti Louis E. Grivetti 《Ecology of food and nutrition》2013,52(4):381-414
The study examined differences in Greek and Greek‐American diets and disease patterns related to migration within the Mediterranean and from the Mediterranean to the United States. Data reported here discuss changes in food patterns and health‐related characteristics of two populations, both originating from the Greek island of Chios, residing either in Athens, Greece, or urban centers in the United States. Food consumption and medical history questionnaires were mailed to 2652 Chians residing in Athens, New York, and the San Francisco Bay region of California. A total of 381 usable responses were obtained; 79% of both the Chian Greek and the Chian Greek American sample were first generation emigrants. Food consumption data were analyzed as monthly frequencies of 49 food items and categories using factor analysis and discriminate analysis. Associations between location of residence and disease prevalence were examined using maximum‐likelihood logit analysis; results were controlled for age, Body Mass Index, physical activity, smoking habit, and socio‐economic status. After controlling for these potential confounders, as well as for presence of the conditions of elevated serum cholesterol, hypertension, and diabetes, Chian American men were identified at higher risk for cardiovascular disease (CVD) than their Athenian counterparts (p < 0.06). This difference in risk for CVD may be related to more frequent use of oils and fats other than olive oil, and less frequent use of olive oil. Among women, diabetes exhibited a positive association with Chian women residing in Athens (p < 0.09), which may be explained by a less frequent use of foods rich in complex carbohydrates by Athenian women than by their American counterparts. 相似文献
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Catherine J. Fedorka Antonia F. Chen William M. McGarry Javad Parvizi Brian A. Klatt 《Clinical orthopaedics and related research》2011,469(4):1024-1032