全文获取类型
收费全文 | 29544篇 |
免费 | 2314篇 |
国内免费 | 102篇 |
专业分类
耳鼻咽喉 | 233篇 |
儿科学 | 899篇 |
妇产科学 | 746篇 |
基础医学 | 4344篇 |
口腔科学 | 438篇 |
临床医学 | 3464篇 |
内科学 | 6101篇 |
皮肤病学 | 570篇 |
神经病学 | 2892篇 |
特种医学 | 725篇 |
外科学 | 2839篇 |
综合类 | 247篇 |
一般理论 | 31篇 |
预防医学 | 3236篇 |
眼科学 | 462篇 |
药学 | 2003篇 |
中国医学 | 47篇 |
肿瘤学 | 2683篇 |
出版年
2023年 | 228篇 |
2022年 | 427篇 |
2021年 | 861篇 |
2020年 | 554篇 |
2019年 | 860篇 |
2018年 | 884篇 |
2017年 | 663篇 |
2016年 | 728篇 |
2015年 | 810篇 |
2014年 | 1064篇 |
2013年 | 1406篇 |
2012年 | 2232篇 |
2011年 | 2243篇 |
2010年 | 1218篇 |
2009年 | 1025篇 |
2008年 | 1805篇 |
2007年 | 1857篇 |
2006年 | 1852篇 |
2005年 | 1703篇 |
2004年 | 1622篇 |
2003年 | 1465篇 |
2002年 | 1288篇 |
2001年 | 381篇 |
2000年 | 329篇 |
1999年 | 401篇 |
1998年 | 275篇 |
1997年 | 212篇 |
1996年 | 195篇 |
1995年 | 180篇 |
1994年 | 138篇 |
1993年 | 135篇 |
1992年 | 181篇 |
1991年 | 212篇 |
1990年 | 201篇 |
1989年 | 189篇 |
1988年 | 166篇 |
1987年 | 165篇 |
1986年 | 154篇 |
1985年 | 180篇 |
1984年 | 139篇 |
1983年 | 119篇 |
1982年 | 77篇 |
1981年 | 80篇 |
1980年 | 75篇 |
1979年 | 100篇 |
1978年 | 71篇 |
1977年 | 63篇 |
1974年 | 66篇 |
1973年 | 79篇 |
1972年 | 64篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
Tamas Papp Angela Polyak Krisztina Papp Zoltan Meszar Roza Zakany Eva Meszar-Katona Palne Terdik Tünde Chang Hwa Ham Szabolcs Felszeghy 《International journal of oral science》2016,8(1):24-31
Although several heat shock proteins have been investigated in relation to tooth development, no available information is available about the spatial and temporal expression pattern of heat shock protein 60 (Hsp 60). To characterize Hsp 60 expression in the structures of the developing tooth germ, we used Western blotting, immunohistochemistry and in situ hybridization. Hsp 60 was present in high amounts in the inner and outer enamel epithelia, enamel knot (EK) and stratum intermedium (SI). Hsp 60 also appeared in odontoblasts beginning in the bell stage. To obtain data on the possible effect of Hsp 60 on isolated lower incisors from mice, we performed in vitro culturing. To investigate the effect of exogenous Hsp 60 on the cell cycle during culturing, we used the 5-bromo-2- deoxyuridine (BrdU) incorporation test on dental cells. Exogenously administered Hsp 60 caused bluntness at the apical part of the 16.5-day-old tooth germs, but it did not influence the proliferation rate of dental cells. We identified the expression of Hsp 60 in the developing tooth germ, which was present in high concentrations in the inner and outer enamel epithelia, EK, SI and odontoblasts. High concentration of exogenous Hsp 60 can cause abnormal morphology of the tooth germ, but it did not influence the proliferation rate of the dental cells. Our results suggest that increased levels of Hsp 60 may cause abnormalities in the morphological development of the tooth germ and support the data on the significance of Hsp during the developmental processes. 相似文献
992.
Now how do we get them home? Outpatient care of pediatric patients on mechanical circulatory support
下载免费PDF全文
![点击此处可从《Pediatric transplantation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Jennifer Conway Christina VanderPluym Aamir Jeewa Selvi Sinnadurai Amanda Schubert Angela Lorts 《Pediatric transplantation》2016,20(2):194-202
The last five yr have been monumental for the pediatric heart failure community. In the US, the most notable has been the FDA approval of the first pediatric specific device (Berlin Heart EXCOR®; Berlin Heart, Inc., Berlin, Germany). Subsequently, the field of heart failure has gained a great deal of knowledge regarding the nuances of MCS in children. Despite FDA approval in the US, the Berlin EXCOR® is only currently indicated for in‐hospital use. Due to the limitations with discharge and the positive in‐ hospital experiences with the Berlin EXCOR®, there has been an increased interest in the implantation of adult durable devices into children. While many institutions have focused their intial efforts on the first phase of care within the hospital, they are now ready to tackle the challenge of how to safely transition children to the community setting. 相似文献
993.
Characterising the safety of clomiphene citrate in male patients through prostate‐specific antigen,haematocrit, and testosterone levels
下载免费PDF全文
![点击此处可从《BJU international》网站下载免费的PDF全文](/ch/ext_images/free.gif)
994.
Sradha Kotwal Angela C Webster Alan Cass Martin Gallagher 《Nephrology (Carlton, Vic.)》2016,21(6):457-466
Linked health data bring together data about one person from varying sources such as administrative health datasets, death registries and clinical registries using a process that maintains patient privacy. Linked health data have been used for burden of disease estimates and health‐care planning and is being increasingly use as a research methodology to study health service utilisation and patient outcomes. Within Australian nephrology, there has been limited understanding and use of linked health data so far, but we expect that with the increasing availability of data and the growing complexity of health care, the use of such data will expand. This is especially pertinent for the growing elderly population with advanced kidney disease, who are poorly represented in other types of research studies. This article summarizes the history of linked health data in Australia, the nature of available datasets in Australia, the methods of access to these data, privacy and ethical issues, along with strengths, limitations and implications for the future. 相似文献
995.
HIV‐1 DNA dynamics and variations in HIV‐1 DNA protease and reverse transcriptase sequences in multidrug‐resistant patients during successful raltegravir‐based therapy
下载免费PDF全文
![点击此处可从《Journal of medical virology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
996.
997.
James M Hotaling Darshan P Patel Christopher Vendryes Natalya A Lopushnyan Angela P Presson Chong Zhang Charles H Muller Thomas J Walsh 《Asian journal of andrology》2016,18(1):35-38
Our objective was to identify predictors of improved postthaw semen quality in men with testicular cancer banking sperm for fertility preservation. We reviewed 173 individual semen samples provided by 67 men with testicular germ cell tumor (TGCT) who cryopreserved sperm before gonadotoxic treatment between 1994 and 2010 at our tertiary university medical center. Our main outcomes measures were independent predictors for the greater postthaw total motile count (TMC) in men with TGCT. Men with NSGCT were more likely to be younger (P < 0.01) and had high cancer stage (II or III, P < 0.01) compared with men with seminoma. In our multiple regression model, NSGCT histology, use of density gradient purification, and fresh TMC > median fresh TMC each had increased odds of a postthaw TMC greater than median postthaw TMC. Interestingly, age, advanced cancer stage (II or III), rapid freezing protocol, and motility enhancer did not show increased odds of improved postthaw TMC in our models. In conclusion, men with TGCT or poor fresh TMC should consider preserving additional vials (at least 15 vials) before oncologic treatment. Density gradient purification should be routinely used to optimize postthaw TMC in men with TGCT. Larger, randomized studies evaluating cancer stage and various cryopreservation techniques are needed to assist in counseling men with TGCT regarding fertility preservation and optimizing cryosurvival. 相似文献
998.
Weekly cisplatin and radiotherapy for low risk,locoregionally advanced human papillomavirus–positive oropharyngeal squamous cell carcinoma
下载免费PDF全文
![点击此处可从《Head & neck》网站下载免费的PDF全文](/ch/ext_images/free.gif)
999.
1000.
Pieles G Geyer SH Szumska D Schneider J Neubauer S Clarke K Dorfmeister K Franklyn A Brown SD Bhattacharya S Weninger WJ 《Journal of anatomy》2007,211(1):132-137
Rapid and precise phenotyping analysis of large numbers of wild-type and mutant mouse embryos is essential for characterizing the genetic and epigenetic factors regulating embryogenesis. We present a novel methodology that permits precise high-throughput screening of the phenotype of embryos with both targeted and randomly generated mutations. To demonstrate the potential of this methodology we show embryo phenotyping results produced in a large-scale ENU-mutagenesis study. In essence this represents an analysis pipeline, which starts with simultaneous micro-magentic resonance imaging (microMRI) screening (voxel size: 25.4 x 25.4 x 24.4 microm) of 32 embryos in one run. Embryos with an indistinct phenotype are then cut into parts and suspect organs and structures are analysed with HREM (high-resolution episcopic microscopy). HREM is an imaging technique that employs 'positive' eosin staining and episcopic imaging for generating three-dimensional (3D) high-resolution (voxel size: 1.07 x 1.07 x 2 microm) digital data of near histological contrast and quality. The results show that our method guarantees the rapid availability of comprehensive phenotype information for high numbers of embryos in, if necessary, histological quality and detail. The combination of high-throughput microMRI with HREM provides an alternative screening pipeline with advantages over existing 3D phenotype screening methods as well as traditional histology. Thus, the microMRI-HREM phenotype analysis pipeline recommends itself as a routine tool for analysing the phenotype of transgenic and mutant embryos. 相似文献