先天性巨大动静脉瘘并血管瘤一例报告

先天性动静脉瘘少见。最近我们收治一例,病变累及右臀及右下肢,臀部并发巨大血管瘤,临床罕见,手术治愈出院。随防二年健在。现报告如下:病例简介患者,女,17岁,右臀包块及右下肢增粗9年,门诊诊断“神经纤维瘤”,于1987年10年收住院。患者于1978年始,于右臀、右小腿外侧出现一包块,渐增大,近三年生长更快,患部感酸痛,坠     

抑肽酶对血小板粘附功能保护作用的实验研究

在正常人富血小板血浆中加入尿激酶后,激活瑞斯托霉素诱导的血小板凝集率下降到对照值的31.7%,表明血小板粘附功能受到明显损伤。这种作用可能与活化的纤溶系统对血小板膜糖蛋白Ⅰ受体的水解有关。事先经抑肽酶孵育的富血小板血浆中加入相同浓度的尿激酶,瑞斯托霉素诱导的血小板凝集率下降不明显,表明抑肽酶可有效地抑制尿激酶的损伤作用,保护血小板的粘附功能。     

正常钙浓度心肌保护液较低钙心肌保护液对不成熟心肌有更好保护作用

虽然不成熟心肌比成熟心肌较能耐受缺血,但在缺血停跳时应用局部低温、晶体心肌保护液或低钙含血心肌保护液,均不能使复跳后心肌功能完全恢复。鉴于不成熟心肌对细胞外钙有较大的依赖性,作者比较了低钙和正常钙浓度心肌保护液对不成熟心肌的保护作用。方法取出生后24～48小时的乳猪32只,分成4组,每组8只。I组:低钙(0.6mmol/L)含血心肌保护液,II组:正常钙(1.1mmol/L)含血心肌保护液;III组;Wisconsin心肌保护液: IV组;正常钙(1.lmmol/L)     

TISSUE-ENGINEERED GRAFT CONSTRUCTED BY SELF-DERIVED BONE MARROW MONONUCLEAR CELLS AND HETEROGENEOUS ACELLULARIZED TISSUE MATRIX

Objective To create a method for constructing a tissue-engineered graft with self-derived bone marrow cells and heterogeneous acellular matrix. Methods The mononuclear cells were isolated from bone marrows drawn from piglets and cultured in different mediums including either vascular endothelial growth factor （VEGF） or platelet derived growth factor BB （PDGF-BB） to observe their expansion and differentiation. The aortas harvested from canines were processed by a multi-step decellularizing technique to erase. The bone marrow mononuclear cells cultured in the mediums without any growth factors were seeded to the acellular matrix. The cells- seeded grafts were incubated in vitro for 6 d and then implanted to the cells-donated piglets to substitute parts of their native pulmonary arteries. Results After 4 d culturing, the cells incubated in the medium including VEGF showed morphological feature of endothelial cells （ECs） and were positive to ECs-specific monoclonal antibodies of CD31, FLK-1, VE-Cadherin and vWF. The cells incubated in the medium including PDGF-BB showed morphological feature of smooth muscle cells （SMCs） and were positive to SMCs-specific monoclonal antibodies of α-SMA and Calponin. One hundred days after implantation of seeded grafts, the inner surfaces of explants were smooth without thrombosis, calcification and aneurysm. Under the microscopy, plenty of growing cells could be seen and elastic and collagen fibers were abundant. Conclusion Mesenchymal stem cells might exist in mononuclear cells isolated from bone marrow. They would differentiate into endothelial cells or smooth muscle cells in proper in vitro or in vivo environments. The bone marrow mononuclear cells might be a choice of seeding cells in constructing tissue-engineered graft.     

老年人体质指数和血脂异常的剂量反应关系研究

目的 探讨60岁及以上老年人体质指数(BMI)与血脂异常患病关联强度的剂量-反应关系。方法 采用整群抽样的方法,以广东省中山市2个镇60岁及以上老年人作为研究对象。使用非条件logistic回归和限制性立方样条模型分析老年人BMI与血脂异常的患病关联和剂量-反应关系。结果 研究人群的血脂异常患病率为39.65%。调整混杂因素后,超重(OR=1.48,95%CI:1.35～1.63)、肥胖(OR=1.79,95%CI:1.55～2.07)和老年人血脂异常患病具有统计学意义。限制性立方样条分析结果显示,随着老年人BMI增加血脂异常风险总体呈上升趋势,当老年人BMI>28 kg/m²时,血脂异常患病风险上升趋势明显,老年人BMI与血脂异常的患病的关联强度呈非线性剂量-反应关系(非线性检验P<0.001)。结论 超重/肥胖是老年人患血脂异常的独立危险因素,老年人的BMI与血脂异常患病风险存在剂量依赖性,研究表明在老年人血脂异常防控中应重点关注BMI的具体数值,识别中高风险人群,而非传统BMI水平(消瘦、正常、超重、肥胖)。     

人CD55基因的克隆及其转基因构件的组装

本实验采用人肝组织作为RNA的来源 ,经RT -PCR扩增得到CD55基因的cDNA片段。与人α -珠蛋白启动子及其polyA序列重组 ,插入质粒载体pGEM - 5zf,获得了可用于受精卵原核显微注射的基因构件 ,为建立人CD55转基因动物模型奠定了基础。     

CD59分子及其在转基因动物中功能表达

体内存在多种可溶性以及膜结合的补体调节蛋白（ｃｏｍｐｌｅｍｅｎｔｒｅｇｕｌａｒｐｒｏｔｅｉｎ，ＣＲＰ），它们以特定方式与不同的补体成份相互作用，使补体的激活与抑制处于精细的平衡状态。在生理条件下，抑制补体的激活可抑制对自身细胞的破环，维护内皮的完整性...     

CD59基因的亚克隆

目的"构建pUC18-α珠蛋白DNA-CD59cDNA重组分子. 方法"采用人心肌组织作为RNA的来源,经RT-PCR扩增得到CD59基因的cDNA片段.以pUC18质粒为载体;人α-珠蛋白DNA为启动子,并提供polyA+尾巴;以编码全部CD59蛋白质的核苷酸序列的cDNA作为目标基因,三者拼接成重组分子. 结果"经酶切鉴定,重组分子拼接成功. 结论"该重组分子可作为转基因构件,经微注射输入供体动物受精卵细胞内,建立人CD59转基因动物模型.     

B超对腹腔镜切除胆囊难易程度的探讨

对我院2000年1月～2001年6月行腹腔镜胆囊切除4601例的术前超声诊断与腹腔镜胆囊切除(LC)结果进行分析如下：