多发性肌炎的CT应用价值

目的研究多发性肌炎(PM)的肌肉CT表现特点及应用价值。方法经肌肉活检证实的多发性肌炎20例。所有病例均完成腰背肌、臀肌、大腿肌以及小腿肌的CT轴位扫描,其中大腿肌和小腿肌分肌群组观察。观察内容包括:肌肉的密度;肌肉的体积;肌筋膜的改变;肌肉组织的清晰度;皮下脂肪层厚度。结果CT发现肌肉体积缩小15个部位;肌肉密度减低,脂肪样变53个部位;筋膜增厚18个部位;皮下脂肪层增厚23个部位;肌肉组织模糊3个部位。结论CT能够清楚显示PM肌肉病损程度、判断肌肉萎缩和病变分布情况,为肌肉活检明确定位。     

幽门螺杆菌沙鼠感染模型的定量分析研究

目的　建立幽门螺杆菌 (Helicobacterpylori,Hp)蒙古沙鼠感染模型 ,并通过比较定量培养、快速脲酶试验和ELISA三种方法 ,确定Hp动物模型的最佳评判指标。方法　采用临床分离的Hp强毒株对 2 0只蒙古沙鼠进行感染 ,4周后剖杀。取血按常规ELISA法检测抗Hp抗体 ;分别剪取胃窦、胃体和胃底粘膜组织作定量培养 ;其余组织作快速脲酶试验及病理检查。结果　经定量培养结果显示沙鼠的Hp感染率达到 80 % (16/ 2 0 ) ,胃窦、胃体和胃底的Hp定植密度的对数均值分别为 5 2 4± 1 5 0、4 11± 3 2 2、和0 97± 2 3 9;16只Hp阳性沙鼠中脲酶试验有 4只阳性 ,ELISA有 7只阳性 ,阳性鼠的感染均值显著高于阴性鼠。结论　经筛选的Hp强毒株成功感染沙鼠后 4周 ,Hp主要定植于胃窦和胃体部 ;定量培养仍是评价Hp动物模型的最有效方法     

头针治疗脑膜炎后遗症运动性失语

夏××,男,11岁,1983年10月5日初诊,门诊号740827。患儿于四月前高烧、头痛、喷射状呕吐,逐渐转入昏迷。三月前入当地县医院住院治疗,诊断为“急性粟粒性结核”、“结核性     

参赭助运合剂治疗系统性硬化症患者食管病变的疗效观察

目的 探讨参赭助运合剂对系统性硬化症（systemic sclerosis,SSc）患者食管病变的临床疗效.方法 将符合入选标准的64例患者随机分为治疗组（参赭助运合剂＋西沙必利片空白模拟剂）和对照组（西沙必利片＋参赭助运合剂空白模拟剂）,疗程2个月,观察患者临床疗效、食管病变证候积分以及立卧位钡剂食管通过时间、卧位食管钡剂排空指数、食管扩张处最宽横径值等的变化和对患者生存质量的影响.结果 总有效率治疗组（96.9%）优于对照组（56.2%,P＜0.01）;两组治疗后食管病变证候均比治疗前均明显改善（P＜0.01）,且治疗组优于对照组（P＜0.01）;治疗组立卧位钡剂食管通过时间、卧位钡剂排空指数均改善（P＜0.01）,食管横径无明显改善;对照组除卧位钡剂食管通过时间有改善（P＜0.01）外,其余指标均无明显变化;治疗组生存质量明显改善（P＜0.01）,对照组则无明显变化.结论 参赭助运合剂可以增强SSc患者食管动力功能,是治疗SSc食管病变的有效中药复方.     

低碘膳食对大鼠脑组织同源盒基因Nkx-6.1和Nkx-6.2 mRNA表达的影响

Objective To study the influence of low-iodine diet on the expression of homeobox gene Nkx-6.1 and Nkx-6.2 in rat cerebrum tissue, and to explore the possible molecular mechanism of cerebrum development retardation caused by low-iodine. Methods Twenty female Wistar rats were randomly equally divided into two groups: low-iodine group and control group, both fed with low-iodine diet as low as 13.66 μg/kg determinated by spectrophotometry in Tianjin Institute of Endocrinology and the former with deionized water, the later 200 μg/L potassium iodate. Thyroid hormone level was detected using chemiluminescence immunoassay 3 months later and they were mated with male rats normally fed. Rats of 16-day pregnancy, new-born and 20th days old were detected the content of Nkx-6.1 and Nkx-6.2 mRNA in the cerebrum tissue by real-time fluorescence quantitative PCR 0.61), (3.28±0.80)pmol/L] were lower than the control group[(1.04±0.06), (39.42±14.68)nmol/L, (4.83±0.33), day pregnancy, new-born and 20th days old of control group was (1.90±0.23)×10-3,(1.86±0.40)×10-4, (1.11± 0.27)×10-4(F=827.58, P<0.01), Nkx-6.1 mRNA expression level gradually decreased along with aging(all P<0.05). The intra-group difference was significant (F=297.25, P<0.01) and the Nkxr.1 mRNA expression level during 16 days of pregnancy was the highest(P<0.01). It was higher in the control group than in the low-iodine group during 16 days of pregnancy (t=10.14, P<0.01) as well as in the low-iodine group than in the in 16-day pregnancy, new-born and 20th days old of control group was respectively(1.03±0.19)×10-2, (1.33± 0.10)×10-3, (8.79±0,87)×10-3, and that of low-iodine group was (0.31±0.03)×10-2, (1.53±0.13)×10-3, (7.51±0.86)×10-2. The intra-group difference was significant(F=1293.02,1065.83, all P<0.01). Nkx-6.2 expression level during 20th days old was the highest(P<0.01) and that of newborn was the lowest(P<0.01). The Nkx6.2 mRNA expression level in control group were higher than the low-iodine group during 16-day pregnancy and 20th days old(t=14.35, 4.05, all P<0.01). It was higher in the low-iodine group than in the control group during newboru(t=4.78, P<0.01). Conclusions The difference in the expression of Nkx-6.1 and Nkx-62 is highly related to the brain development retardation caused by low-iodine.     

ERCC1表达在Ⅲ期非小细胞肺癌含铂方案新辅助化疗中的意义

目的 探讨Ⅲ期非小细胞肺癌(NSCLC)患者中人切除修复交叉互补因子1(ERCC1)表达与含铂新辅助化疗敏感性及预后的关系.方法 27例行新辅助化疗患者为实验组,33例同期未行新辅助化疗者为对照组,应用免疫组化法检测两组ERCC1表达.实验组患者于新辅助化疗前后均行CT检查.结果 实验组中ERCC1阳性和阴性患者中位生存时间分别为11、29个月(P<0.05).化疗后有效率分别为21.4%、69.2%(P<0.05).胸部CT检查示新辅助化疗有效率为44.4%.ERCC1表达与有效率具有相关性(r=0.487 6,P<0.01).结论 检测ERCC1表达有助于预测Ⅲ期NSCLC含铂方案新辅助化疗疗效,并与胸部CT检查的化疗有效率相关.     

双启动子shRNA真核表达载体的构建

目的:构建含有双启动子的shRNA真核表达载体.方法:通过PCR分别扩增带有BamH Ⅰ、EcoR Ⅰ酶切位点的人U6启动子序列(U6-B/E)和带有Bgl Ⅱ、HindⅢ酶切位点的人U6启动子序列(U6-B/H),用BamH Ⅰ与EcoRⅠ双酶切pcDNA3.1(+)与PCR产物U6-B/E后进行连接,重组质粒命名为psPRO.再用BglⅡ与HindⅢ双酶切psPRO与PCR产物U6-B/H后进行连接,构建含有双启动子的shRNA真核表达载体.结果:酶切鉴定以及DNA测序结果显示载体构建成功.结论:构建双启动子的shRNA真核表达载体,使其在细胞中表达2个不同的siRNA,不仅筛选方便,而且可以通过建立这种模式化工具载体,为今后RNA干扰实验研究的开展和深入提供手段.     

电针配合康复训练治疗上肢周围神经损伤56例分析

目的分析电针配合康复训练治疗上肢周围神经损伤的疗效。方法将112例上肢周围神经损伤患者随机分为观察组(n=56)与对照组(n=56),前者接受电针配合康复训练治疗,后者接受单纯康复训练治疗,比较2组患者的疗效。结果①观察组患者的感觉功能治疗效果、显著优于对照组,2组相比差异有统计学意义(P<0.05)。②观察组运动功能治疗效果显著、优于对照组,2组相比差异有统计学意义(P<0.05)。③观察组临床疗效显著优于对照组,2组相比差异有统计学意义(P<0.05)。结论电针配合康复训练可显著恢复上肢周围神经损伤患者的感觉功能与运动功能,该方法是治疗上肢周围神经损伤的有效方法之一。     

甲状腺激素对成骨样MG-63细胞甲状腺激素受体不同亚型表达的影响

目的 检测人类成骨样细胞MG-63中甲状腺激素受体(TR)的表达情况以及甲状腺激素(T3或T4)对其的影响.方法 用实时荧光定量PCR法对MG-63细胞中TR的主要4个亚型,α1、α1、β1、β2的mRNA表达进行了测定.结果 TRα1 mRNA的表达水平最高,为10.70±0.45;TRβ1仅及其半,为5.75±0.10;TRβ2不低,相当于TRβ1的60%,为3.34±0.08;TRα2的表达极低,仅为(3.66±0.59)×10-2.分别加入不同浓度的T3或T4,仅TRα1和TRα2对T3表现明显的降调节,且TRα1和TRα2的表达与相应T3浓度之间负相关,其他亚型无显著性变化,也无相关关系;同样浓度范围的T4作用各亚型,表达均无显著性变化.结论 在骨细胞发育分化中TRα1起关键作用.

Abstract：

Objective To examine the expression of thyroid hormone receptor isoforms (TR α1, α2, β1, and β2) in human osteoblast-like cell line MG-63 at the mRNA level and the effect of thyroid hormone (T3 or T4 ) on the expression. Methods Realtime quantitative PCR was performed. Results The expression of TRα1 mRNA was the highest, that was 10. 70± 0.45, TRβ1 was 5.75 ± 0. 10, TRβ2 was 3.34 ± 0. 08, and TRα2 was very low, only (3.66 ±0. 59) × 10-2. Only the expression of TRαl and TRα2 mRNA was down regulated significantly by the treatment of 10-10 ～ 10-6 mol/L T3, and there was a negative correlation between the expression of TRα1 or TRα2 mRNA and the concentration of T3. Conclusion TRα1 plays a primary role in mediating the effects of thyroid hormones in skeletal development.     

负荷剂量氯吡格雷联合阿司匹林治疗TIA的临床研究

短暂性脑缺血发作(transient ischemic attack,TIA)是神经内科常见病之一,通常认为TIA有1/3自行消失,1/3频繁发作,1/3发展为脑梗死.TIA虽症状在短时间内缓解,但预后堪忧,因此积极防治TIA尤为重要.近年来氯吡格雷和阿司匹林联合应用治疗TIA的疗效,得到较为广泛的关注,但其具体应用方法,有效性和安全性还没有形成一致意见.本文观察了100例TIA患者的治疗,负荷剂量氯吡格雷联合阿司匹林的疗效显著,且继发出血等副作用未见增加.现报告如下.