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免疫学是一门生物基础理论科学,也是一门应用科学。近20多年来,由于生物物理学、生物化学、细胞生物学及分子生物学的发展和免疫学技术的进步,临床免疫学得到了长足的发展。免疫病可以认为是一个边缘性疾病,覆盖范围跨越临床各科。随 相似文献
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The hepatocellular carcinoma (HCC) cell line, QGY-7703, derived from a Chinese patient, was used to immunize the BALB/c mice. Fifteen hybridomas producing McAb that reacted with QGY-7703 cells were isolated from 858 hybridomas created in three cell fusions. In further studies two McAb, namely, AQGY1 and A-QGY2, were selected which specifically stained HCC cells grown in vitro. The reactivity of these McAb was not removed by the absorption by homogenates of the normal liver, but was by homogenates of HCC cells. A-QGY1 and A-QGY2 also reacted definitely with HCC cells in liver tissues of HCC patients, but neither with other cells in the tissues nor with nontransformed liver tissues of the same patients. Furthermore these two McAb stained the adult or fetal liver tissues, nor all of the other normal or tumor tissues that had been tested. Blocking and absorbing experiments revealed that A-QGY1 and AQGY2 antigens had no immunohomogenicity with antigens such as HBsAg, HBcAg, HBeAg, AFP and CEA. The specificity of these two McAb may be used potentially for the sero diagnosis, histologic identification, radioimmunoimaging and destruction of human hepatocellular carcinoma. 相似文献
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为了探讨重症乙型肝炎(重乙肝)中HDV感染的检出率及临床特征,作者按1984年全国第三届病毒性肝炎会议拟订的标准,在四川筛选出重乙肝27例(其中22例于死后作了肝穿刺或尸解),用以下方法进行检测。取肝组织,用ABC法检测HDV和HBcAg;Southern吸印杂交法检测HBV-DNA。取血清,用ELISA法检测δ抗体和HBsAg、HBeAg、抗-HBe、抗-HBcIgM;斑点杂交法测HBV-DNA。 27例中肝检测δ抗原者18例,结果2例阳性;血清检测δ抗体者7例,3例阳性;另2例同时检测δ抗原和抗体,均为阳性和均为阴 相似文献
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本文利用生物素标记探针从细胞定位水平对50例原发性肝癌(HCC)手术活检标本进行了HBV DNA检测。 32例(64％)HCC切片中检出HBV DNA,其中25例(50％)系肝癌细胞与癌旁肝细胞内均检出HBV DNA,其余7例则仅在癌旁肝细胞内检出HBV DNA。光镜下观察,HBV DNA的分布具有胞浆内为主;癌旁肝细胞优势性、HCC阳性者同时伴有癌旁肝细胞阳性及不均一性等特点。上述结果提示至少占半数的HCC病例其癌细胞可能来自HBV DNA阳性肝细胞的恶变,但HBV DNA在恶变过程中的确切作用尚需进一步研究。 相似文献
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