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??Abstract??Objective??To analyze the prevalence characteristic of viral diarrhea in Guangzhou. Methods??Totally 985 specimens and condition information were collected from children with acute diarrhea in Guangzhou Children’s Hospital from August 2008 to July 2009?? then real-time fluorescence quantitative PCR was employed to detect HRV?? NORV?? EAdV?? ASTV and SPAV. Results??Among the 985 specimens 452 specimens were positive??in which 103 specimens was found with co-infection?? the annual rate of HRV?? NORV?? EAdV?? ASTV and SPAV was 28.0%?? 13.7 %?? 8.8%?? 4.9% and 1.0%.About 95% of positive samples were from infants under 2 years of age?? the incidence rate of watery stools?? fever and vomit was 88.9%?? 68.4% and 75.2%. Conclusion??Viral diarrhea is the most common cause in infants and young children with acute diarrhea. HRV is the most important pathogens. October to December is the peak incidence. Infants under 2 years is a major pop groups. Watery stools?? fever and vomiting is infantile viral diarrhea triad. Co-infection is common but has little influence on symptoms.     

环氧化物水解酶1基因多态性对华法林剂量影响的研究

 目的 探讨苏南地区心脏机械瓣膜置换术后汉族人群环氧化物水解酶1(EPHX1)基因rs4653436和rs2292566多态性对华法林剂量的影响,为以后利用基因多态性指导临床合理用药提供理论依据。方法 采用碱基淬灭探针技术,检测197名心脏机械瓣膜置换术后患者的EPHX1 rs4653436和EPHX1 rs2292566的基因型,分析对华法林剂量的影响。结果 EPHX1基因 rs4653436多态性检测有127例纯合子GG型,62例患者为AG型杂合子,8例突变纯合子AA型; GG型患者华法林的平均稳定剂量为（2.64±0.93） mg·d^-1, AG型为（2.52±0.68） mg·d^-1,AA型为（3.25±1.63） mg·d^-1,采用方差分析方法得到各组间华法林剂量差异P值大于0.05,无显著性差异;而EPHX1基因 rs2292566多态性检测有107例纯合子GG型,70例AG型杂合子,20例突变纯合子AA型; GG型患者华法林的平均稳定剂量为（2.47±0.85） mg·d^-1,AG型为（2.49±0.77）mg·d^-1,AA型为（2.81±0.96）mg·d^-1,方差分析方法得到各组间华法林剂量差异P值小于0.05,有显著性差异。结论 在苏南地区心脏机械瓣膜置换术后汉族人群中,EPHX1基因 rs4653436多态性与华法林剂量相关性无显著性差异,对华法林剂量影响较小;而rs2292566多态性与华法林剂量相关性有显著性差异,对华法林剂量有影响。     

半枝莲多糖的纯化及对S180荷瘤小鼠的抑瘤作用

目的将半枝莲粗多糖纯化后测定多糖含量,并比较粗多糖与精多糖的抑瘤率,为半枝莲化学及药理研究提供有益的参考。方法采用分光光度法、苯酚-硫酸显色法,在490nm处测定其含量;并利用体内实验测定多糖的抑瘤效果。结果精多糖的多糖含量为54．12％,平均加样回收率为99．66％,粗多糖的抑瘤率为45．96％,精多糖的抑瘤率为22．14％。结论测定多糖含量的方法操作简便,准确可靠,精多糖的抑瘤效果没有粗多糖抑瘤效果明显。     

间氟苯乙醛缩乙醇胺席夫碱抑菌活性研究

目的试验新合成的化合物间氟苯乙醛缩乙醇胺席夫碱对枯草杆菌等五种细菌的抑菌作用,为寻找具有抑菌、抗癌、抗病毒、杀霉等生物活性的药物提供新药。方法采用国际药典通用的管碟法。首先培养枯草杆菌、大肠杆菌、大肠杆菌(101)、金黄色葡萄球菌、革兰阴性细菌(发荧光Q67)等五种细菌,取其第二代繁殖体作受试对象。将间氟苯乙醛缩乙醇胺席夫碱用适量蒸馏水溶解,配制浓度为0.25,0.5,1,2mg/mL一系列溶液,加样后除革兰阴性细菌(发荧光Q67)在25℃培养箱里培养24 h,其余均在室温下培养24 h,实验中以蒸馏水作为对照。结果作为对照的蒸馏水对上述细菌无任何抑制作用,该席夫碱对上述细菌均有不同程度的抑制作用。结论抑菌活性实验表明该席夫碱对枯草杆菌、大肠杆菌、大肠杆菌(101)、金黄色葡萄球菌、革兰阴性细菌(发荧光Q67)等五种细菌均有较好的抑菌作用。     

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??Clinical pathological characteristics of double primary cancers of gastric cancer and breast cancer??A report of 25 cases WANG Chao-ying*, LI Hui-ping, XIE Yun-tao, et al. *Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Breast Oncology, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing 100142, China
Corresponding author: SHEN Lin, E-mial:lin100@medmail.com.cn
Abstract Objective To investigate clinicopathological characteristics of double primary cancers of gastric cancer and breast cancer, so as to provide reference for following study of secondary primary tumor and (or) multiple primary cancers(MPC). Methods The clinicopathological data of 25 cases of double primary cancers of gastric cancer and breast cancer admitted from January 2001 to June 2013 in Beijing Cancer Hospital were analyzed. Among 25 cases, 6 cases had ovary carcinoma and (or) other malignant tumors. Results The middle onset age of the 1st primary cancer, mainly breast cancer, was 45 (30-66) years old, while that of the 2nd primary cancer was 54 (35-74) years old, mainly gastric cancer. The main pathology of gastric cancer was diffuse type accounting for 80%, while that of breast cancer was invasive ductal cancer accounting for 88%. The middle interval between the two cancers was 49 ??6.5-243.0??months. Conclusion Double primary cancers of gastric cancer and breast cancer have the characteristics of young onset age, short interval between two primary cancers and comorbidity with other primary cancers and family history, which indicates genetic susceptibility may play a role. For the treatment, early detection and early radical surgery can only prolong the survival.     

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??Diagnostic and therapeutic endoscopy for biliary stones following bilioenterostomy??A report of 17 patients MEI Jia-wei*, ZHANG Yi, ZHANG Wen-jie, et al. *Department of General Surgery, Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China
Corresponding author: WANG Xue-feng, E-mail??wxxfd@live.cn
Abstract Objective To evaluate the successful rate, safety and efficiency of endoscopic retrograde cholangiopancreaticography (ERCP ) for biliary stone following bilioenterostomy. Methods The clinical data of 17 patients with biliary stone following bilioenterostomy underwent ERCP from April 2012 to March 2014 in Xin Hua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine were analyzed retrospectively. Results Among 17 patients, 13 patients were performed Roux-en-Y bilioenterostomy and 4 patients were performed duodenopancreatectomy. In 14 of 17 patients, afferent loop intubation and selective cannulation of biliary duct was successful in 82.35% and 100% respectively. Procedure of diagnosis was carried out in 4 patients, and therapeutic in 10 patients who underwent stone removal+ endoscopic nasobiliary drainage (ENBD). There was no complication of perforation and bleeding in all patients. Conclusion ERCP for biliary stone following bilioenterostomy is safe and efficient.     

HPLC-ELSD测定不同产地吉祥草中凯提皂苷元的含量

 目的 建立吉祥草中凯提皂苷元的含测定方法。方法 采用高效液相色谱（HPLC）-蒸发光散射检测器(ELSD ),Agilent Ecplise XDB-C₁₈ (4.6 mm×150 mm , 5 μm)柱,甲醇-水(50∶50)为流动相,流速为1.0 mL · min-1,柱温25 ℃,载气压力0.33 MPa,漂移管温度65 ℃。结果 凯提皂苷元质量浓度在0.1~1.5 mg·mL-1之间线性关系良好,r=0.999 5,平均回收率 98.4%, RSD=1.5% (n=3)。结论 该方法分离效果好,操作简便,稳定性好,重复性好,可用于吉祥草中凯提皂苷元的含测定,为吉祥草药材的开发利用奠定基础。