同种异体骨移植的免疫反应

早在 18 81年Ｍａｃｅｗｅｎ[1] 就最先报道了他在异体骨移植方面的成功经验。本世纪初 ,Ｌｅｘｅｒ[2 2 ] 又将全膝关节和半膝关节移植用于临床 ,并对患者进行了随访 ,有将近一半的膝关节数年后仍保持了良好的功能。一个世纪以来骨组织的低温保存技术、冷冻前保护技术以及手术技巧都有了显著的提高 ,如今同种异体骨移植已成为临床矫形和整形外科常用的治疗手段。1　异体骨移植免疫学研究背景尽管颗粒状异体骨在良性骨囊肿刮除后骨腔填塞、脊柱融合等治疗中成功率已达到 90 %以上 ,然而对于将大块异体骨关节移植用于肿瘤保肢、关节形成、创…     

骨肉瘤患者血清中整合素α2β1的表达水平

目的 检测骨肉瘤患者血清中整合素α2β1的表达,探讨其与骨肉瘤生物学行为的关系及在治疗前后和肺转移时的变化.方法 应用酶联免疫吸附技术检测43例骨肉瘤患者(病例组)术前及术后、化疗前及化疗后血清整合素α2β1含量,男28例,女15例;年龄8～47岁,平均(18.42±9.10)岁;骨母细胞型24例,纤维母细胞型9例,软骨母细胞型7例,其他3例;EnnekingⅡA期13例,ⅡB期26例,Ⅲ期4例;其中22例患者接受新辅助化疗.26例志愿者为正常对照组,男16例,女10例;年龄18～32岁,平均(23.54±3.82)岁.病例组分别根据肿瘤大小、原发部位、临床分期、组织学分型以及转移情况进行分组,比较各组血清中整合素α2β1表达的差异.结果 病例组患者术前血清整合素α2β1为(2.44±0.89)μg/L,显著高于对照组(1.85±0.43)μg/L.术前表达水平与骨肉瘤Enneking临床分期、肿瘤大小和远处转移存在相关性,而与肿瘤原发部位及Dahlin组织学分型无关.骨肉瘤保肢术后患者整合素α2β1的血清浓度降低.接受新辅助化疗的患者化疗前后血清中整合素α2β1表达水平的差异具有统计学意义.结论 整合素α2β1的表达与骨肉瘤的发生、发展、转移呈正相关,可能成为一项新的诊断及评估骨肉瘤预后的生物学指标.

Abstract：

Objective To evaluate the expression of Integrin α2β1 in serum of osteosarcoma patients and to investigate the effect of Integrin α2β1 on metastasis of osteosarcoma. Methods Sandwich enzymelinked immunosorbent assay was applied to test Integrin α2β1 in serum of 26 healthy controls (male 16, female 10; age 18-32, average age 23.54±3.82) and 43 osteosarcoma patients (male 28, female 15;age 8-47,average age 18.42±9.10)before and after the surgery and the chemotherapy. The patients included osteoprogenitor cells type in 24 cases, fibroblast type in 9 cases, chondroblast type in 7 cases, and other types in 3cases; with Enneking stage ⅡA in 13 cases, stage ⅡB in 26 cases, stage Ⅲ in 4 cases. Among them, 22 patients receive the neo-adjuvant chemotherapy. After that, we compared the data of different groups. At the same time, in accordance with tumor size, position, clinical stage, histology grade and metastasis situation to categorize groups compared among the groups of serum Integrin α2β1 differences in order to explore its relationship with patients' prognosis and metastasis. Results The Integrin α2β1 of osteosarcoma patients in blood before the surgery was expressed highly. It was significantly higher than the control group. The expression before surgery had significant correlation with osteosarcoma Enneking clinical stages, tumor size and metastasis. And it was independent of tumor position and Dahlin histology grade. The expression of Integrin α2β1 in blood of the osteosarcoma patients after limb salvage surgery decreased significantly. And the expression of Integrin α2β1 in blood of patients receiving new adjuvant chemotherapy was significant differences before and after the chemotherapy. Conclusion The expression of Integrin α2β1 has positive correlation with the occurrence, development and metastasis of the osteosarcoma. It is probably a new biological indicator for diagnosis and estimating prognosis of osteosarcoma. Simultaneously surgery and new adjuvant chemotherapy are still effective methods to treat osteosarcoma.     

游离型腰椎间盘突出症的诊断与手术治疗

目的探讨游离型腰椎间盘突出症的临床诊断及手术治疗方法。方法通过对16例游离型腰椎间盘突出的患者进行手术治疗,评估影像学诊断意义,通过随访对治疗效果进行评价。结果核磁共振检查可明显提高游离型腰椎间盘突出症的诊断准确率,积极进行手术后可获得80%的治疗效果优良率。结论游离型腰椎间盘突出症存在一定的发生率,诊断明确后应早期手术,对于术前存在腰椎不稳、腰椎间盘手术术后复发、全椎板切除的情况可考虑同时行该节段的固定融合术。     

骨肉瘤临床预后相关分子因素的研究进展

骨肉瘤作为一种在儿童和年轻人中最常见的恶性骨肿瘤,具有高度恶性、血运丰富、高转移率、预后差等特点.虽然已经使用了新辅助化疗和手术治疗相结合的方式,但总的5年无瘤生存率大约也只有65%[1].     

在人体脊柱皮肤上记录脊电图方法初步报告

16例脊髓神经系统无器质性病的正常人和2例腰椎间盘突出,疑有脊髓迫压的病人,作体表脊电图检查。采用双极导联将直径1×0.8cm银片电极涂导电胶固定在L_3·L_4·L_5·脊柱皮肤上,记录信号经前置放大器送入KDS—1A生理数据处理机、经A/D转换叠加处理,采样时间50μs,起始延迟1ms,叠加128～256次。     

巨大髂骨骨血管瘤1例

患者男性，43岁，农民，左腹股沟处无痛性包块逐渐增大10年，无痛。入院时体检：全身一般情况良好，左腹股为至左季肋区有一包块约20cm×30cm×40cm，前内侧超过腹中线，上部超过脐部，后侧超过腋后线，界限清，活动度差，无波动感，表面硬度不均，有部分骨块感，与皮肤无粘连，皮肤温度不高，无血管怒张。左下肢末梢血循环良好，感觉运动无异常，大、小便无异常。血象正常，血沉6mm／h，AKP（碱性磷酸酶）10U。X线片：左髂翼部骨质破坏，软组织巨大包块。于1994年3月25日在硬膜外麻醉下行包块切除术。组织学检查见大量扩张的血窦，大小不…     

Effect of type I collagen on the adhesion, proliferation, and osteoblastic gene expression of bone marrow-derived mesenchymal stem cells

tificially synthesizedpolymericproductssuchaspolylactide (PLA ) ,polyglycolide (PGA ) ,andtheircopolymersarefrequently usedtissueengineeringmatrixsubstitutivematerials .Unfortunately ,theirapplicationisseriouslyrestrictedfortheirpoorhydrophilicproperty ,weakcelladsorptionforces ,anddifficultyininteractionwithcells .1,2 ThisstudyintendstoevaluatethecapacityofcollagenIinpromotingadhesion ,proliferation ,anddifferentiationofMSCs ,tolayanexperimentalbasisforbonytissueengineering .METHODSPrep…     

S100A4在骨肉瘤侵袭和转移中的作用

肺转移是骨肉瘤患者治疗失败和死亡主要原因,S100A4是S100家族成员之一,主要源于上皮细胞和肿瘤细胞,它在体内具有多种生物学功能,目前的研究主要关注其与肿瘤的侵袭、转移的关系及机制,本文就S100A4的分子生物学特点及其在骨肉瘤侵袭、转移作用做一综述.     

α2整合素抗体对人类骨肉瘤MG63细胞迁移侵袭的影响

目的:细胞间黏附分子整合素的变化与肿瘤细胞的侵袭迁移之间有重要联系,文中进一步深入探讨α2整合素抗体对骨肉瘤细胞侵袭和迁移能力的影响,为预防骨肉瘤细胞的转移和辅助治疗提供理论依据. 方法:采用细胞迁移实验、细胞侵袭实验观察骨肉瘤MG63细胞在整合素α2单克隆抗体作用下瘤细胞迁移、侵袭能力的变化.迁移或侵袭的细胞通过结晶紫染色,100倍显微镜下计数. 结果:anti-α2阻断组细胞的迁移能力较对照组明显降低,侵袭能力较对照组明显减弱. 结论:α2整合素抗体在骨肉瘤细胞迁移侵袭过程中起着抑制作用.