Application of Color Doppler Ultrasonic Examination in Deep Vein Valvular Incompetence in Lower Limbs

Inthepast,rep0rts0ndiagn0sisofdeepvaIvularincompetence(DVI)inl0werlimbbycol0rDopplerflowimaging(CDFI)coveredonlytheobservationonthemor-phologicandhemodynam1cparametersandthequantitativedeterminationofveindiam-eter,flowvelocltyhasnotbeenreported.Moreover,noconsistentstandardwasavailable-Inthisstudy,theDVIwasdiag-nosedbyusingCDFI,combinedwithdeepvenographyandotherclinicalexaminati0nsandonthebasisofthechangesinvesseIm0rph0logyandhem0dynamics.Thedeepveinvalvefunctioninlowerlimbswasex-amined…     

Correlation of cytotoxic effect of transmembrane and secretory TNF-α to cell cycle

This study was aimed to examine the correlation of the cytotoxic effects induced by two types of TNF-α to cell cycle. Hoechst 33342 and PI were used to detect the morphological changes in the cell death induced by the two types of TNF-α. TdT and PI co-staining was performed to determine the phase of cell cycle of apoptotic cells. L929 cells in different phases of cell cycle were further synchronized and their sensitivity to the two types of TNF-α was observed. Our results showed that the apoptosis of HepG2 cells triggered by tm-TNF-α mainly occurred in G1 phase while in HL-60, Raji and K562 cell lines it mainly took place in S phase. The apoptosis of L929 cells induced by tm-TNF-α mainly occurred in S phase while the apoptosis induced by s-TNF-α mainly appeared in G1 phase. L929 cells were sensitive to s-TNF-α when synchronized in G1 phase (cytotoxicity 49.8%) while their sensi-tivity to tm-TNF-α was highest in S phase (45.7%) and G1/S phase (cytotoxicity 40.6%). It was concluded that tm-TNF-α-induced apoptosis of different target cells took place in different phases of cell cycle. The apoptosis of the specific cell line induced by the two types of TNF-α occurred in different phases of cell cycle. The sensitivity of the specific cell line to the two types of TNF-α was correlated with the phase of cell cycle.     

线粒体融合素-2对裸鼠移植瘤Ki-67、血管内皮生长因子的影响

目的:探讨瘤内注射vivo-jetPETTM/pEGFP Mfn2复合物对裸鼠移植瘤增殖的影响.方法:质粒pEGFPMfn2的构建及检测,将MCF-7细胞异种移植到裸鼠体内,建立人乳腺癌移植瘤模型,用vivo-jetPEITM试剂制备vivo-jetPEITM/DNA复合物,实验共分为实验组、空载体对照组和空白对照组.瘤内多点注射,RT-PCR检测肿瘤组织线粒体融合素-2(Mfh2)的表达,免疫组化检测Ki-67、血管内皮生长因子(VEGF)的表达情况.结果:pEGFP Mfn2构建成功并测序证实,实验组的相对平均吸光度值为(1.12±0.05)高于空载体对照组的(0.16±0.05)和空白对照组(0.17±0.07)(P<0.05),Ki-67在实验组的相对平均吸光度为(0.25±0.06)高于空载体对照组的(0.11±0.02)和空白对照组(0.12±0.03)(P<0.05),而VEGF在实验组的相对平均吸光度为(0.09±0.01)低于空载体对照组的(0.14±0.08)和空白对照组的(0.13±0.03)(P<0.05).结论:vivo-jetPEITM试剂能成功将Mfn2基因转染进瘤体细胞,Mfn2基因对人乳腺癌裸鼠移植成瘤后的Ki-67和VEGF有影响.     

炎性乳腺癌的治疗新进展

炎性乳腺癌是一种特殊的局部晚期乳腺癌,肿瘤侵袭性和转移性强,病死率非常高.由于炎性乳腺癌恶性程度极高,病情发展迅速,早期即有全身广泛转移而危及生命,预后很差.目前临床上主要采取以手术、放疗、化疗等综合治疗措施来治疗这类疾病,但是治疗效果极其有限.而现代分子生物学和肿瘤免疫学领域的突破进展为这类疾病的治疗带来了希望.本文...     

乳腺癌组织中paxillin和c-erbB-2的表达及临床意义

目的：研究paxillin蛋白和c—erbB-2蛋白在乳腺癌组织中的表达，探讨其与乳腺癌患者临床病理因素的关系，以评价乳腺癌的预后。方法：采用免疫组织化学SP法检测53例原发性乳腺癌、10例乳腺纤维腺瘤及10例距癌灶边缘〉5cm病理组织学检查正常的乳腺组织中paxillin和c—erbB－2蛋白的表达情况。结果：与正常乳腺组织和乳腺纤维腺瘤相比，乳腺癌中paxillin表达显著刚氏（P=0．003），而c—erbB－2表达显著增高（P〈0．001）。paxillin的表达与患者的年龄、绝经状况、肿瘤细胞的ER和PR表达及肿瘤大小无关，而与病理学分期、组织学分级和淋巴结转移有关；c—erbB－2表达与患者的年龄、绝经状况、肿瘤大小、PR表达和病理学分期无关，而与ER表达、组织学分级和淋巴结转移有关。paxillin和c—erbB－2的表达无相关性。结论：paxillin和c—erbB－2与乳腺癌细胞的恶性转化、侵袭转移有关，检测paxillin和c—erbB－2蛋白表达情况有助于准确评价乳腺癌患者的预后。     

触诊阴性乳腺病灶的诊治体会(附85例分析)

目的探讨触诊阴性乳腺病灶的临床表现特点和诊治方法。方法回顾性分析85例触诊阴性乳腺病灶的临床资料。结果触诊阴性乳腺病灶一般没有明显临床症状,少数有乳房胀痛、乳头溢液（血）或乳房外观改变,临床上诊治相对困难。85例中良性病变69例,占81．2％,恶性病变16例,占18．8％。结论通过超声引导下或X线立体定位针吸细胞学检查、穿刺病理学检查、手术活检以及乳腺导管镜检查对触诊阴性乳腺病灶基本可以做出明确诊断。     

低位领式切口保留颈丛择区性颈侧方淋巴结清扫术在甲状腺乳头状癌中的应用

目的 总结低位领式切口保留颈丛的择区性颈侧方淋巴结清扫术（颈清术）在甲状腺乳头状癌中的应用和经验.方法 2011年1月至2013年8月对66例乳头状癌患者行全甲状腺切除和低位领式切口保留颈丛的择区性颈清术.结果 Ⅵ区淋巴结转移率为69.7％,颈侧方淋巴结（Ⅱ、Ⅲ、Ⅳ、Ⅴb）转移率为72.7％,主要集中在Ⅲ和Ⅳ.患者术后无明显下颈部、耳部和肩部感觉异常,随访1 ～ 30个月无局部复发.结论 临床怀疑或穿刺证实颈侧方淋巴结转移的乳头状癌患者,在没有广泛淋巴结转移（Ⅴa转移）或淋巴结明显外侵的情形下,低位领式切口保留颈丛的择区性颈清术良好的兼顾了肿瘤根治和功能外观,是一种安全而合理的术式.