ULTRASTRUCTURAL CHARACTERISTICS OF NEPHROBLASTOMAS AND THEIR RELATION TO DNA PLOID

Fifty-one cases of nephroblastoma were pathologically studied by light and electron microscopy as well as microspectrophotometry. Under light and electron microscope, the tumor appeared to be carcinosarcoma structurally, consisting of epithelial, sarcomatous and undlfferentiated tumor cells. Their ultrastructural feature was similar to that of renal tubular epithelial cells and tibroblast. It could differentially be diagnosed from renal metoblastic tumors by electron microscopy when the tumor was mainly composed of sarcomatous cells. Microspectrophotometer measurement showed an Increased mean nuclear DNA content (DI>1. 04), belonging to aneuplold tumor, which together with polyploid cells, serving as one of the Important characteristics of malignant tumors. In addition, the poorer the tumor differentiation the higher was the DNA content of the tumor cells, suggesting that the measurement of nuclear DNA of tumor cells might also serve as one of signs to acertain the degree of differentiation and malignan     

电视宫腔镜在宫内节育器异位诊治中的应用

我院应用电视官腔镜对疑难的宫内节育器(IUD)异位患者13例进行检查、诊断、治疗，取得满意效果，现将临床资料总结如下。     

KALA-AZAR INFECTED SERUM CIRCULATING ANTIGENSAND THEIR CHARACTERISTICS DETECTED BYMONOCLONAL ANTIBODY*

Lcishmania donovani antigens were successfullyidentified in the sera of kala-azar paticnts by mono-clonal antibody (McAb). The serum antigens wereabsorbed onto nitrocellulose paper, then bound tohigh titcr McAb-C2 against L. donovani and detected by ELISA (McAbAST). Of 51 serum samplcstested, 48 were McAb-AST positive, while sera from 367 controls (normal individuals from kala.azarendemic or non-endemic areas and patients withother infectious or parasitic diseases) were all nega-tive. The difference between positive and negativercsults (ELISA color reaction expressed in ISA) wasdistinct and the positive degree (+-十++) decrcas-cd with the increase of serum dilu:tioii. : Followup study of 8 kala-azar cases pre- and p~ specific therapy demonstrated that McAb-AST results correlated well with their clinical symptoms and L. donovani amastigote findings. The circulating antigens in sera of kala-azar patients were found tobc gfycoprotcins by PAS stain and trypsin digestion.They separated int0 4 bands (Rf 0.35, 0.25, 0.20 andO.O6) by PAGE. By SDS-PAGE, they migrated as 3bands (MW 96, 52 and 30 KD). Their isoelectricpoint (pl) as determined by IEF was between pH8.71-9.16 and showed 7 bands. These findings in-dicate that the circulating antigens were a group ofglycoproteins with different molecular weights andpfs.     

酸性染料萃取法测定麦得灵滴眼液中盐酸麻黄碱的含量

酸性染料萃取法测定麦得灵滴眼液中盐酸麻黄碱的含量杨快儿，洪俊荣１试药和仪器盐酸麻黄碱（药典规格）；氢溴酸后马托品（符合四川省药品标准）；麦得灵滴眼液（本院自制产品）；以上试药均由本院制剂室提供。溴麝香草酚蓝试液：按中国药典９０版配制；ｐＨ５．８磷酸盐...     

对新生儿及孕妇测量腋温时间的探讨

目前,临床工作中测量体温的时间大致相同,如测最腋温为5～10 min。为了查明体温计在新生儿及孕妇腋下停止上升的时间(稳定时间),找出其中的规律,我们对400人次的新生儿和204人次的孕妇进行了测试观察,现将结果报告如下。     

顺从他的纵欲让我悔恨终身

一个平凡的女人不要有太多幻想和追求,当你处在我的角度,就会感觉此生最大的幸福就是做一个平凡而伟大的母亲。     

职场无间道

职场如战场，这句话并非危言耸听。跻身于职场，每天面对着不同的人与事，在八卦与新闻中度日，自需有一些防身之处。