宫颈环形电切术治疗宫颈上皮内瘤变56例临床分析

[目的]探讨宫颈环形电切术（LEEP）治疗宫颈上皮内瘤变（CIN）的疗效。[方法]总结分析LEEP治疗CIN56例的临床资料。[结果]LEEP治愈CIN51例,治愈率为91.07%,其中CIN1级治愈率达100%。56例CIN患者术中出血量2～15ml,平均4.6ml。术后并发症主要为阴道不规则出血。[结论]LEEP治疗CIN疗效确切,具有操作简单、诊断准确、安全有效、出血量少等优点。     

Proteomics in Discovery of Hepatocellular Carcinoma Biomarkers

Objective： To discover new proteomic biomarkers of hepatocellular carcinoma. Methods： Surface enhanced laser desorption/ionization time-of-flight （SELDI-TOF） mass spectrometry was used to discover biomarkers for differentiating hepatocellular carcinoma and chronic liver disease. A population of 50 patients with hepatocellular carcinoma and 33 patients with chronic liver disease was studied. Results： Twelve proteomic biomarkers of hepatocellular carcinoma were detected in this study. Three proteomic biomarkers were highly expressed in hepatocellular carcinoma and nine proteomic biomarkers were highly expressed in chronic liver disease. The most valuable proteomic biomarker with m/z=11498 had no similar diagnostic value as α-fetoprotein. Conclusion： Some of the twelve proteomic biomarkers may become new biomarkers of hepatocellular carcinoma.     

癌结节对胃癌患者生存预后的影响

  目的  探讨癌结节对胃癌患者生存预后的影响。  方法  回顾性分析2007年1月至2012年12月海南医学院第一附属医院收治的312例胃癌患者的临床病理资料,根据术后病理标本中有无癌结节将所有患者分为癌结节阴性组和阳性组。分析癌结节与临床病理因素的关系及其对胃癌患者生存预后的影响。  结果  本组患者中84例存在癌结节,阳性率为26.9%。单因素分析显示,Borrmann分型、肿瘤大小、浸润深度、N分期、TNM分期、脉管癌栓与癌结节阳性相关;多因素分析证实仅Borrmann分型、N分期和脉管癌栓是癌结节阳性的独立相关因素。单因素生存分析显示,年龄、肿瘤部位、Borrmann分型、肿瘤大小、TNM分期、术式、脉管癌栓和癌结节与胃癌患者预后相关。癌结节阴性组和阳性组5年生存率分别为67.5%和34.5%,差异有统计学意义（P < 0.001）。多因素生存分析显示年龄、Borrmann Ⅲ/Ⅳ型、TNM分期、脉管癌栓和癌结节是患者的独立预后因素。进一步分层分析显示,癌结节仅对N0~3a期胃癌患者预后影响有统计学意义,N3b期癌结节阴性组和阳性组患者预后无显著差异。癌结节阳性患者预后与N分期、有无脉管癌栓及术后辅助化疗独立相关。  结论  癌结节是胃癌患者独立预后因素,可作为N0~3a期患者预后评价指标,无论分期如何,癌结节阳性者需行术后辅助化疗并密切随访。      

颞浅动脉岛状皮瓣眉再造同时纠正睑外翻临床观察

颜面深度烧伤愈后 ,眉缺损合并睑外翻是临床常见的畸形 ,不仅影响美观 ,而且由于睑裂闭合不全、致使睑结膜、角膜裸露 ,常可导致暴露性睑结膜炎、角膜炎 ,甚至失明等严重并发症。故需及时手术治疗。手术不仅要达到功能上的保护性修复 ,还要兼顾美观的要求。我们用颞浅动脉顶支或额支皮瓣眉再造同时纠正睑外翻 3例 ,效果显著。1　手术方法1 1　术前应进行眉定位、剃除毛发 ,若双眉均缺失时 ,可根据患者面部的形态、特点、眼裂大小设计眉形并用笔描出 ,使眉与眼和睑形和谐 ,病人满意 ,并用纸取样。1 2　术前应以多普勒血流探测仪测定颞浅动脉 …     

流行性感冒病毒裂解疫苗在18岁以上健康人群中应用的安全性及免疫原性研究

目的 评价流感病毒裂解疫苗安尔来福(R)的免疫原性及安全性.方法 2010年8-9月在沈阳市开展开放式临床试验,选择18～60岁及＞60岁健康成年人接种安尔来福(R)进行安全性观察,并采集受试者免疫前及免疫后21 d 血清标本,采用血凝抑制试验(HI)进行流感病毒裂解疫苗甲型H1N1、甲型H3N2及乙型3个型别抗体检测.结果 130名观察对象完成疫苗接种并进行安全性观察,其中120人完成免疫前及免疫后采血.总体不良反应发生率为2.3%(3/130),均为全身不良反应,未出现严重不良反应.接种疫苗后21 d,成年组甲型H1N1、甲型H3N2及乙型3个型别抗体阳转率分别为82.5%、93.7%、92.1%,GMT增长倍数分别为20.2、32.0、11.4,保护率分别为92.1%、98.4%、98.4%;老年组3个型别抗体阳转率分别为89.5%、91.2%、87.7%,GMT增长倍数分别为23.9、39.8、15.1,保护率分别为93.0%、94.7%、96.5%.结论 疫苗安尔来福(R)接种后甲型H1N1、甲型H3N2及乙型3个型别抗体各项指标均超过欧盟标准,表明其免疫原性及安全性良好.

Abstract：

Objective To evaluate the safety and immunogenicity of split influenza vaccine (Anflu(R) ). Methods An open-labeled clinical trial was carried out in adults aged 18-60 years and elders aged over 60 years from August to September, 2010 in Shenyang, Liaoning province. One dose of split influenza vaccine was administered and adverse events were observed. Serum samples were obtained prior to vaccination and 21 days post vaccination. A/H1N1, A/H3N2 and B antibodies against influenza virus were measured using micro-hemagglutination inhibition (HI) assay. Results A total of 130 subjects were recruited and 120 paired serum samples were obtained. The overall rate of adverse events was 2.3% (3/130) and all of them with systemic reaction. No single serious adverse event was reported. 21 days after the vaccination, the sero-conversion rates of A/H1N1, A/H3N2 and B antibodies against influenza virus among adults were 82.5%, 93.7% and 92.1%, respectively. The Geometric Mean Titer (GMT) ratios were 20.2, 32.0 and 11.4, while the sero-protection rates were 92.1%, 98.4% and 98.4%, respectively. The sero-conversion rates of antibodies among elders were 89.5%, 91.2% and 87.7%, with the GMT ratios as 23.9, 39.8 and 15.1, respectively. The seroprotection rates were 93.0%, 94.7% and 96.5%,respectively. Conclusion All indexes ofA/H1N1,A/H3N2 and B antibodies exceeded the licensure criteria established by the EU Committee for Medicinal Products for Human Use,proving the trial vaccine Anflu(R) with good safety and immunogenicity.     

小议脑梗死与糖尿病相关性分析

脑梗死与糖尿病都是临床常见病,目前两者同时存在更是屡见不鲜.脑梗死是糖尿病的常见及严重并发症,是导致糖尿病患者生存质量下降的主要原因,它也是不少糖尿病患者最早的临床表现.高血糖不但可影响全身各组织器官的营养代谢,它对中枢神经系统的损害也越来越受到重视.     

泌尿道感染常见病原菌分布及其耐药性检测

为了解我院近年泌尿道感染的病原菌分布及耐药情况,笔者对我院2007年1月-2009年12月门诊及病房泌尿道感染的患者中段尿的病原菌及药敏进行分析,报告如下。     

益气补肾强底汤联合电刺激生物反馈治疗产后盆底功能障碍70例

女性盆底功能障碍(PFD)临床主要表现为性功能障碍、盆腔器官脱垂及压力性尿失禁等,为妇产科多发病.因妊娠及分娩过程的腹腔压力过大导致盆底结构发生变化或者损伤会阴部神经、盆底肌肉和尿道支撑组织而出现盆底功能障碍性疾病.据统计,约有50％的女性在妊娠生育后会出现程度不同的PFD,严重影响女性产后的身心健康和生活质量[1]....     

万托林联合普米克吸入辅助治疗小儿哮喘26例疗效分析

目的：观察万托林联合普米克治疗小儿哮喘的临床疗效。方法：所有病例入院后在抗感染及补液的基础上,加用万托林雾化溶液,〈3岁者剂量为0．25ml;〉3岁者剂量为0．50ml。而普米克液内含布地奈德雾化混悬液．〈3岁者剂量为0．25ml：〉3岁者剂量为0．50ml,与万托林共同加入生理盐水2～3ml,重度小儿哮喘每天3次,中度小儿哮喘每天2次,疗程为2～3d。2-3个月后改为每天1喷,持续6～18个月,直至哮喘完全消失。结果：26例中,无效2例（7．69％）,有效15例（57．69％）,显效9例（34．62％）,总有效率为92．31％。结论：两药合用可控制哮喘急性发作,逐步减少哮喘发作次数,并最终根治哮喘,是日前较理想、较安全的用药。     

慢性宫颈炎的疗效观察及护理体会

目的:分析射频消融术对慢性宫颈炎治疗疗效,并总结相关护理方法。方法:60例慢性宫颈炎患者随机分组为试验组和对照组各为30例。对照组:采用微波治疗和进行常规护理。试验组:在对照组治疗和护理基础上,采用射频消融术治疗,并进行针对性护理。结果:试验组恢复时间和不良反应以及复发率等要明显优于对照组,P0.05。结论:对慢性宫颈炎患者采用射频消融术治疗,并对患者进行针对性的心理等护理值得推广。