转录因子Ets-1与肝纤维化关系的研究

转录因子Ets-1是Ets家族成员之一,与肝纤维化的关系日益受到人们的关注,它通过调节多种因子的基因转录,从而影响肝纤维化的发生和发展,本文将Ets-1在肝纤维化中的作用概述如下。     

蝎毒活性肽对福尔马林痛实验的影响

中医用全蝎或蝎尾治疗风湿痹痛、惊厥等症［１］。现代医学证明蝎毒具有抗肿瘤、抗炎、抗癫痫、镇痛等作用［２］。本实作者单位　４５３００３　河南新乡　新乡医学院医学系１９９６级（冯　晓，祝　琳，范书锋）；新乡医学院生理学教研室（张一红，丁延峰）验通过福尔马林痛模型，观察由小鼠腹腔注射蝎毒活性肽后的镇痛效应。１　材料与方法１．１　蝎毒活性肽　由新乡医学院肿瘤研究所提供，用ｐＨ６．８，浓度为１／１５ｍｏｌ·Ｌ－１的ＮａＨ２ＰＯ４－ＫＨ２ＰＯ４缓冲溶液稀释为浓度０．０１％的溶液。福尔马林溶液用福尔马林原液与…     

穿透性角膜移植治疗感染性角膜溃疡的临床观察

目的： 观察穿透性角膜移植术治疗感染性角膜溃疡的疗效。方法： 采用 Ｍ Ｋ 液保存的新鲜角膜材料对 ２６ 例 （２６ 只眼） 严重的细菌、真菌及单疱病毒性角膜溃疡行穿透角膜移植术, 术后随访 １２～１８ 个月。结果： 细菌、真菌及单疱病毒性角膜溃疡的术后植片透明率分别为 ７７７８％ 、５３８５％ 、２５％ , 术后主要的并发症有一过性高眼压、植片排斥及感染复发。结论： 对于药物治疗无效的严重的感染性角膜溃疡, 穿透性角膜移植可有效去除病灶, 重建眼球的完整性, 并为二期光学角膜移植奠定基础。     

Effect of nuclear factor-κB p65 ASOND on I typ collagen expression and rat hepatic stellate cells proliferation

Objective Sstudy effect of nuclear factor-κB ASOND on I type collagen expression and rat hepatic stellate cells(HSC)proliferation.Methods Rat HSCs were separated by affusing and digestingof Ⅳ type collagenenzyme and density acentric method.Lipid-mediated NF-κB p65 ASOND(0.001,0.01,0.1,1μmol/L)Was transferred into rat HSCs.Toxicity of HSCs caused by NF-κB p65 ASOND and activity of LDH were determined by trypan blue staining.Proliferation affection of transferring NF-κB p65 ASOND into HSCs was determined by MTT.In different concentration NF-κB p65 ASOND.expression of Ⅰ type collagen stimulated by 1mg/L TNF-αwas determined by RT-PCR and ELISA.Results After transfection of NF-κB p65 ASODN,expression of NF-κB protein in HSCs was decreasing.Toxicity experiment indicated that NF-κB p65 ASOND of different concentration(0.001,0.01,0.1 and 1.0 μmol/L)had no effect on HSCs livability and LDH activity(P<0.05).Four different concentration NF-κ B p65 ASOND could restrain HSCs proliferation stimulated by 1 mg/L TNF-α.The expression of I type collagen and mRNA stimulated by 1mg/LTNF-αwas increased,and had a positive correlation with concentration(P>0.05).Conclusion NF-κB p65 ASOND may depress NF-κB activity to restrain HSCs proliferation and Ⅰ type collagen expression,and reduce extracellular matrix.     

Effect of nuclear factor-κB p65 ASOND on I typ collagen expression and rat hepatic stellate cells proliferation

Objective Sstudy effect of nuclear factor-κB ASOND on I type collagen expression and rat hepatic stellate cells(HSC)proliferation.Methods Rat HSCs were separated by affusing and digestingof Ⅳ type collagenenzyme and density acentric method.Lipid-mediated NF-κB p65 ASOND(0.001,0.01,0.1,1μmol/L)Was transferred into rat HSCs.Toxicity of HSCs caused by NF-κB p65 ASOND and activity of LDH were determined by trypan blue staining.Proliferation affection of transferring NF-κB p65 ASOND into HSCs was determined by MTT.In different concentration NF-κB p65 ASOND.expression of Ⅰ type collagen stimulated by 1mg/L TNF-αwas determined by RT-PCR and ELISA.Results After transfection of NF-κB p65 ASODN,expression of NF-κB protein in HSCs was decreasing.Toxicity experiment indicated that NF-κB p65 ASOND of different concentration(0.001,0.01,0.1 and 1.0 μmol/L)had no effect on HSCs livability and LDH activity(P<0.05).Four different concentration NF-κ B p65 ASOND could restrain HSCs proliferation stimulated by 1 mg/L TNF-α.The expression of I type collagen and mRNA stimulated by 1mg/LTNF-αwas increased,and had a positive correlation with concentration(P>0.05).Conclusion NF-κB p65 ASOND may depress NF-κB activity to restrain HSCs proliferation and Ⅰ type collagen expression,and reduce extracellular matrix.     

新型农村合作医疗基金安全管理的财务思考

新型农村合作医疗已在部分地区开始试点,从实际调查的情况分析,基金安全管理方面,制度尚欠规范。本文从财务的视角,探索这一新型农村合作医疗基金的安全建设。     

蛋白质组学技术鉴定吉兰-巴雷综合征患者血清急性期反应蛋白的表达

背景：吉兰-巴雷综合征患者血液中存在与发病有关的抗体、补体和细胞因子,以蛋白质组学技术分离鉴定这些标志蛋白,可为寻找新的药物靶标提供依据。目的：以蛋白质组技术比较吉兰-巴雷综合征患者与正常对照组血清的差异表达蛋白。方法：采集确诊的吉兰-巴雷综合征患者和正常者血清各30例,提取血清蛋白质以固相pH梯度等电聚焦为第一向,SDS-PAGE垂直电泳为第二向进行双向电泳,图象分析软件Imagemaster 2D分析电泳图谱,MALDI-TOF/TOF串联质谱鉴定差异表达蛋白。结果与结论：在吉兰-巴雷综合征患者与正常者中24种蛋白质的表达量显著不同,其中α-2-巨球蛋白,血浆铜蓝蛋白,血清淀粉样P物质,丛生蛋白,抗糜蛋白酶,触珠蛋白,血红素蛋白,α-1-抗胰蛋白酶,血清转铁蛋白等9种蛋白质被鉴定为急性期反应蛋白。结果说明吉兰-巴雷综合征患者血清中急性期反应蛋白表达量发生了明显变化,加深了对吉兰-巴雷综合征发病分子机制的理解。     

慢性扩展性脑内血肿2例临床分析

目的:探讨慢性扩展性脑内血肿(CEICH)疾病的病因、病理学特征、临床表现及影像学改变。方法:通过报道我院两例慢性扩展性脑内血肿病例的临床、病理及影像学资料,并结合相关文献复习。结果:多数CEICH病变因其临床表现及影像学特征等与颅内占位性病变极为相似,故术前易误诊为颅内肿瘤或脓肿等。结论:临床工作中如发现颅内占位性病变,临床表现为慢性起病、头痛、癫痫等以及影像学提示环状强化明显时应考虑慢性扩展性脑内血肿的可能,但手术所见及病理学报告仍是目前诊断的主要依据。     

上海市老年人桡、尺骨骨矿含量分析和绝经对妇女骨矿含量的影响

应用单光子吸收技术调查分析了60岁以上男性578名、女性557名桡、尺骨骨矿含量与绝经对妇女骨矿含量的影响。结果表明60岁以上的骨矿含量较30～89岁明显降低,女性年丢失率为男性的2～8倍,随增龄女性骨矿低于男性的程度越益明显,绝经年限对骨矿含量有阴显影响。老年人骨矿丢失的特点为老年保健提出了新的课题。     

一例过量医疗照射诱发肉瘤

郭某，女，９岁。辽宁锦州人。左大腿放射性溃疡伴肉瘤。１９８９年２月某日午夜因发热在当地卫生院作肌肉注射时，误将针头断在臀部肌肉内，在Ｘ射线透视下手术取针约３～４小时，致局部组织过量受照。一年后（１９９０年）受照部位逐渐形成硬块、隆起。１９９５年１１月...