ANALYSIS OF APOPTOSIS BY DNA END LABELING METHOD (TDT) IN LEUKEMIA CELL LINES HL-60 AND U937

Apoptosisisakindofcelldeathrecognizablebyaseriesofspecializedchangesinmorphological,biochemicalandmolecularbiologicalforms.Apoptosisisanactiveprocessofcelldestruction,characterizedbycellshrinkage,chromatinaggregationwitheXtensivegenomicfragmentation,crescentbodyformingbychromosomepyknosisalongthenuclearmargin.Intheapoptosis,endouncleaseasedependentCa2 andMg2 isactivated,thisenzymemakechromosomedegradedatthelinkersectionstofragmentsequivalenttosingleandmultiplenucleosomes.Thelatterarerevealeda…     

CLINICAL OBSERVATION OF THE SEGMENTAL TYPE OF ADENOMYOMATOSIS OF THE GALLBLADDER CAUSING GALLBLADDER CARCINOMA

CLINICALMATERIALSFrom1975to1993,68patientswithadenomyomatosisofthegallbladderwereconfirmedbyoperationatBeijingRailwayGeneralHospital,representing1.7%ofthetotalbenignlesionofthegallbladderinthesameterm,lowerthan8.7%whichwasreportedbyOofani.1Theaverageagewas57.8years,maleversusfemalewasI.4:1.Itwasclassifiedilltothreetypesaccordingtothegrossfeaturesofthespecimensbythenakedeyeduringoperation:x1.SegmentalType:44cases,64%ofthetotal.Acircularnarrowwascomposedinthemiddleofthegallbladderanddi…     

DETECTION OF HUMAN TELOMERASE ACTIVITY BY TELOMERASE TRAP-ELISA ASSAY

ＤＥＴＥＣＴＩＯＮＯＦＨＵＭＡＮＴＥＬＯＭＥＲＡＳＥＡＣＴＩＶＩＴＹＢＹＴＥＬＯＭＥＲＡＳＥＴＲＡＰ—ＥＬＩＳＡＡＳＳＡＹＷｅｉＬｉｘｉｎ卫立辛ＷｕＭｅｎｇｃｈａｏ吴孟超ＹａｎＺｈｅｎｌｉｎ阎振林ＳｈｅｎＦｅｎｇ沈锋ＸｉｅＴｉａｎｐｅｉ谢天培Ｑｉａ...     

结直肠癌伴穿孔的诊治体会

目的：探讨有效地预防结、直肠癌伴穿孔的发生及合理治疗结、直肠癌伴穿孔的方法。方法：用X2检验分析742例患者的5年生存率与其中的11例伴穿孔患者的5年生存率（所有病例均随访5年以上）。结果：全部742例结、直肠癌患者的5年生存率为58.6％。11例伴穿孔患者的5年生存率仅为18.2％。4例伴急性穿孔患者中的3例有诱发穿孔的因素。穿孔的部位大多发生在左侧结肠；其病理类型多数为粘液腺癌。结论:结、直肠癌伴穿孔患者的预后很差，在诊治过程中避免医源性的诱发穿孔因素值得重视；特别是结、直肠癌伴不全性梗阻时，要慎重选择检查手段及清洁肠道准备的方法。     

气滞胃痛冲剂药理学实验研究

气滞胃痛冲剂ig,能明显抑制小鼠胃排空和小肠推进作用,减少胃液分泌,降低胃液酸度及胃蛋白酶活性,增强家兔在体肠蠕动强度,对大鼠幽门结扎型溃疡有明显的保护作用,还有明显的镇痛作用。     

针刺对小白鼠实验性乳腺增生症抑制作用的病理形态定量研究

对雌二醇（Ｅ２）造模的小鼠乳腺，采用连续纵切片方法，研究乳腺腺泡的病变程度与针刺作用的关系。结果表明：乳腺腺泡的改变及增生程度与针刺的抑制作用有显著的相关性。说明针刺确有抑制Ｅ２所致实验性乳腺增生作用。     

The relationship between dietary fat intake and risk of colorectal cancer: evidence from the combined analysis of 13 case-control studies

The objective of this study was to examine the effects of the intakeof dietary fat upon colorectal cancer risk in a combined analysis of datafrom 13 case-control studies previously conducted in populations withdiffering colorectal cancer rates and dietary practices. Original datarecords for 5,287 cases of colorectal cancer and 10,470 controls werecombined. Logistic regression analysis was used to estimate odds ratios (OR)for intakes of total energy, total fat and its components, and cholesterol.Positive associations with energy intake were observed for 11 of the 13studies. However, there was little, if any, evidence of anyenergy-independent effect of either total fat with ORs of 1.00, 0.95, 1.01,1.02, and 0.92 for quintiles of residuals of total fat intake (P trend =0.67) or for saturated fat with ORs of 1.00, 1.08, 1.06, 1.21, and 1.06 (Ptrend = 0.39). The analysis suggests that, among these case-control studies,there is no energy-independent association between dietary fat intake andrisk of colorectal cancer. It also suggests that simple substitution of fatby other sources of calories is unlikely to reduce meaningfully the risk ofcolorectal cancer.     

血小板α颗粒膜蛋白140检测在“病”与“证的”特异性联系

应用血小板α颗粒膜蛋白１４０（ＧＭＰˉ１４０）为血小板活化指标，测定了７４例糖尿病、３６例心血管疾病、５３例肾脏疾病患者的血浆ＧＭＰˉ１４０浓度，并分别按病种及按是否有血瘀证分组比较了各组间的ＧＭＰˉ１４０测定值。结果表明：ＧＭＰˉ１４０在各疾病组患者均较正常人升高，三组间则无明显差异；而在血瘀及非血瘀证明之间则有明显差异。认为ＧＭＰˉ１４０有可能作为诊断血瘀证的一项实验室指标。     

GM-CSF/IL-4基因修饰的瘤苗不同的免疫途径诱导不同免疫反应的观察

对细胞因子基因修饰瘤苗的研究表明,IL-2、IL-4、IFN-γ、GM-CSF等一系列细胞因子基因以不同载体转入肿瘤细胞制成瘤苗后皮下免疫小鼠,均可增强机体抗肿瘤免疫力,其机制可能是由于瘤苗分泌的细胞因子促进了免疫细胞对肿瘤抗原的识别、提呈及对肿瘤细胞的杀伤能力.有文献报道,逆转录病毒介导的GM-CSF和IL-4共同转染瘤苗可以有效激发机体抗肿瘤免疫力,为探讨不同途径瘤苗免疫后机体的免疫反应,我们采用皮下、腹腔、脾内、静脉四种途径接种GM-CSF、IL-4基因双重转染的小鼠红白血病细胞FBL-3瘤苗,发现免疫途径不同,所激发的免疫应答类型不同,诱导机体生成的免疫力不同,提示某些瘤苗应用时应选择适当的免疫途径.     

结直肠癌组织微量元素的分析电镜研究

目的：探讨结、直肠癌组织中的微量元素变化。方法；应用分析电镜对37例结、直肠癌，移行粘膜和正常粘膜组织中元素进行研究。结果；在结、直肠癌中磷元素和钾元素相对含量显著高于正常粘膜（P＜0．05）。结论：上述结果提示在结、直肠癌中除微量元素钙、铜、锌和锰含量降低以外，元素磷和钾含量增加。