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991.
992.
Polymorphism of ACE gene and its relationship with serum ACE activity in the Hans in Chengdu region]
This study was aimed at the angiotensin converting enzyme (ACE) gene insertion/deletion(I/D) polymorphism distribution in Chinese and its relationship with serum ACE activity(SACE). SACE activity and ACE gene polymorphism in 90 healthy persons in Chengdu region were investigated using a spectrophotometric assay and the PCR technique, respectively. The results showed that, in the Hans, DD genotype of ACE gene was 18%, DI genotype 44%, and II genotype 38%. Allele frequencies were 0.40 for the D allele and 0.60 for the I allele. There were significant relationships between ACE I/D polymorphism and SACE activity. The results suggest that ACE genotype distribution be different between races, that II genotype and I allele be more frequent in Chinese, and that SACE activity be under the influence of a genetic polymorphism of ACE in Chinese. 相似文献
993.
The method for detecting the number of living cells was studied. Using an adenosine triphosphate (ATP) bioluminescence assay, the present authors reported a perfect linear relationship between lg ATP concentrations and lg luminescence counts (r = 0.9963) as well as a relationship between lg number of cells and lg ATP luminescence counts (r = 0.9922). The detectable cells ranged from 10(2) to 10(6) cells/ml, the coefficients of variation 1-3%. This method is simple, accurate and sensitive and has a high reproducibility. 相似文献
994.
Neurofibromatosis is a dominantly inheriteddefect of the ectoderm,characterized by cafe au laitspots since the childhood and multiple tumorsderived from elements of peripheral and cranialnerves.Hernia-like tumors of various sizes can oftenbe felt under the skin and serve as a valuablecharacteristic in making diagnosis.However,theycan also affect brain,vegetative nerve system or othertissues and lead to bony abnormalities,mentaldisturbances and sometimes,endocrine abnormalities, 相似文献
995.
The aim of this study was to assess the value of malignant disease-associated DNA-binding protein 2(MAD2) in the diagnosis of liver cancer. The concentration of plasma MAD2 was determined in 27 patients with primary liver cancer, 14 patients with metastatic liver cancer and 12 healthy subjects by ELISA assay. The primary tumors of all the patients with metastatic liver cancer were located in the gastrointestinal tract, which had been radically resected and had no signs of local recurrence. The concentrations of plasma MAD2 in the patients with primary liver cancer, with metastatic liver cancer, and the healthy subjects were 30.56 +/- 11.38 micrograms/ml, 9.27 +/- 5.58 micrograms/ml and 8.43 +/- 5.62 micrograms/ml, respectively. The concentration of MAD2 in patients with primary liver cancer was significantly higher than that in the patients with metastatic liver cancer and healthy subjects (P < 0.001). There was no significant difference between the healthy subjects and the patients with metastatic liver cancer(P > 0.05). These results suggest that MAD2 may be an useful marker for the diagnosis of primary liver cancer. 相似文献
996.
为探讨绒毛膜促性腺激素(hCG)作用于胃腺癌细胞后的信号传递机制,我们对hCG对胃腺癌SGC-7901细胞胞浆内cAMP含量的影响进行了观察。结果显示:当hCG的浓度由10^-11mol/L增加到10^-6mol/L时,胞内cAMP的水平从2.1pmol/mg蛋白增至约10pmol/mg蛋白(P〈0.01),与对照组相比有显著性差别(P〈0.01)。同时,此效应还具有时间依赖性,即随着hCG作用时 相似文献
997.
目的:探讨CD44S和CD44V6在胃淋巴瘤中的表达特征及其临床意义。方法:采用HE染色、CD3、CD20、CD44S和CD44V6的经法观察47全。 低度恶 胃淋巴瘤中的阳性率为工恶生为62%,差异显著(P=0.02),CD44V6在低度胃淋巴瘤中的阳性率为6%,高度恶性为38%,差异显著(P=0.01)。CD44S在临床1期的阳笥率为30%,2~4期为75%,差异显著(P-0-.002)。结论 相似文献
998.
Luminol-hemin system was used for chemiluminescent flow-injection analysis with immobilized glucoxidase(GOD) column to determine blood glucose. The method is fast, simple and sensitive. Blood samples were diluted to 1:1000 and pumped into the flow-injection analyzer for direct detection. The sampling frequency was 30 samples/h, with a detection limit of 7.2 x 10(-9) mol/L(1.30 micrograms/L). A column packed with 36 milligrams of CPG could be used nearly 200 times. The determination results of glucose in serum by this method were in good agreement with those obtained by the routine GOD spectrophotometric method (correlation coefficient r = 0.90, P > 0.05). Recoveries of this method were 94.0%-107.2%. 相似文献
999.
为观察苏林酸对结肠腺癌HT-29细胞有无促凋亡作用,采用流式细胞仪检测、DNA电泳和透射电镜观察苏林酸对HT-29细胞的促凋亡作用。结果:流式细胞式、DNA电泳和透射电镜观察均显示苏林酸可诱导HT-29细胞凋亡,且具有时间和剂量依赖性。用0.3mmol/L、0.6mmol/L和1.2mmol/L苏林酸处理48小时,凋亡细胞分别占5.8%、7.6%和11.7%,处理72小时则分别升为12.5%、15 相似文献
1000.
过氧化物诱导LO2细胞凋亡时Fas mRNA表达及Ca^2+流变化的单 … 总被引:1,自引:0,他引:1
探讨过氧化物所致肝细胞凋亡时Fas基因表达与Ca^2+流变化的相互关系。方法应用全细胞膜片错单细胞逆转录聚合酶链反应技术进行H2O2诱导人类正常肝细胞LO2细胞FasmRNA表达的单细胞分析,应用全细胞膜片错显微荧光单细胞胞浆游离钙浓度测量技术、流式细胞术、扫描电镜观察同期瞬时Ca^2+流变化及早期凋亡(Annexin-V)细胞指数和细胞膜变化。结果H2O2作用2h单个LO2细胞电泳图分析可见Fa 相似文献