肝移植后肝窦内皮细胞损伤的研究

目的 探讨肝移植后再灌注损伤和排斥反应时,肝窦内皮细胞的变化。方法 利用抗肝窦内皮细胞的单克隆抗体对大鼠肝移植后定期采取的肝组织标本染色。结果 对照组（LEW－LEW）于第4天在肝窦壁内可见ICAM－1的强染色,但是昆布氨酸、第Ⅷ因子相关抗原在实验基间均未见到。高度排斥反应组（ACI－LEW）移植后第1在开始发现ICAM－1的强染色。中度排斥反应组（BN－LEW）移植后第1天开始发现ICAM－1的强染色,移植后1个月开始肝窦壁出现第Ⅷ因子相关抗原。结论 说明慢性排斥反应引起的肝损伤,不仅是以肝动脉为中心的血管损伤,同时也损伤肝窦内皮细胞。     

顽固性复发性气胸胸膜腔造影及硬化治疗后追踪

对32例顽固性复发性气胸患者用60%泛影葡胺进行胸膜腔造影,确定裂口后作硬化剂治疗,并作复张后胸部CT对照及手术追踪对照,取得满意效果。     

磷酸钛钾激光声带切除术不同术式的临床特点

目的 探讨支撑喉镜下磷酸钛钾（ｐｏｔａｓｉｕｍ ｔｉｔａｎｉｕｍ ｐｈｏｓｐｈａｔｅ,ＫＴＰ）激光声带切除术不同术式的选择及愈合过程中的临床特点。方法 选择７６例早期声门型喉癌行ＫＴＰ激光声带切除术,对不同术式术后喉内结构恢复过程、音质变化等进行随访观察记录。结果 Ⅰ型术式１１例,３个月后恢复喉的正常结构及功能;Ⅱ型术式５０例,３个月后有声带样粘膜皱襞（以下称新声带）形成,基本恢复喉的结构;Ⅲ型术     

青光眼类型及静态阈值视野损害

目的:探讨青光眼类型与静态阈值视野损害间的关系.方法:对确诊的147例、228只青光眼分类进行静态阈值视野检查.结果:不同类型的青光眼在静态阈值视野缺损出现的最大象限上差异无显著性.青光眼静态阈值视野缺损最大象限的分布依次为颞上、鼻上、鼻下,颞下象限分布最少.结论:不同类型的青光眼所致静态阈值视野损害的主要机制可能是相同的.     

McAb共刺激PBLs淋巴细胞在培养体系中的状态及超微结构的 …

通过用抗ＣＤ３,ＣＤ２８＋ＣＤ８０ ＭｃＡｂ激活健康人的ＰＢＬｓ,并以ＰＨＡ,ＩＬ－２,ＰＢＬｓ为对照组;对各组不同时间段的淋巴细胞超微结构进行观察。结果提示：ＣＤ３及ＣＤ２８＋ＣＤ８０刺激淋巴细胞增殖外,也能使淋巴细胞活化,细胞表现为胞体增大,细胞器增多,具有粗大的绒毛和突出伪足,并可见单核细胞吞噬活跃。     

Partial Sequence Analysis of the Genome of Human Herpesvirus 7 YY5 Isolated from Saliva Samples

Ｈｕｍａｎｈｅｒｐｅｓｖｉｒｕｓ 7(ＨＨＶ 7)ｉｓｏｎｅｏｆｔｈｅｍｏｓｔｒｅｃｅｎｔｌｙｄｉｓｃｏｖｅｒｅｄｍｅｍｂｅｒｏｆｔｈｅｈｅｒ ｐｅｓｖｉｒｕｓｆａｍｉｌｙ .Ｉｔｗａｓｆｉｒｓｔｉｓｏｌａｔｅｄｆｒｏｍａｃｔｉｖａｔ ｅｄＣＤ4 Ｔｃｅｌｌｓｆｒｏｍｐｅｒｉｐｈｅｒａｌｂｌｏｏｄｍｏｎｏｎｕ ｃｌｅａｒｃｅｌｌｓ (ＰＢＭＣｓ)ｏｆａｈｅａｌｔｈｙｉｎｄｉｖｉｄｕａｌ[1] ,ａｐａｔｉｅｎｔｗｉｔｈｃｈｒｏｎｉｃｆａｔｉｇｕｅｓｙｎｄｒｏｍｅ(ＣＦＳ) [2 ] ,ａｎｄｆｒｏｍｓａｌｉｖａｏｆｈｅａｌ…     

NXS40D手术X线电视透视机的改造

本文介绍了用1台50mA工频X线机的控制台来改造一废旧中频X线电视透视机的方法,该法简便易行,费用低廉,性能可靠。     

医学大学生对医德认知的调查研究

采用抽样研究方法,对４３８名不同专业、不同层次的大学生进行了医德认知调查,以了解大学生对医德基本知识掌握的情况,结果表明,大学生对开设医学伦理学课必要性认识均率为６２．９％,课外经常阅读医德书籍均率为２２．７５％,对中外基德发展史各朝代典型的人物及代表作了解的均率为３１．１％,文中对调查结果进行了分析。     

Transcriptome analysis of Armillaria gallica 012 m in response to auxin

Gastrodia elata is an achlorophyllous and fully mycoheterotrophic orchid which obtains carbon and other nutrients from Armillaria species in its life cycle. Many researchers suggested that plant hormones, as signing molecules, play a central role in the plant–fungi interaction. In the process of Armillaria gallica 012 m cultivation, both exogenous indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) distinctly stimulated the growth of mycelia in solid media. The differential expression genes (DEGs) of A. gallica 012 m with IAA versus blank control (BK) and IBA versus BK were investigated. The results showed that more than 80% of DEGs of the IAA group were coincident with the DEGs of the IBA group, and more than half of upregulated DEGs and most of the downregulated DEGs of the IAA group coincided with those DEGs of the IBA group. Above research implied that A. gallica 012 m could perceive IAA and IBA, and possess similar responses and signaling pathways to IAA and IBA. The overlapping differential genes of the IAA group and IBA group were analyzed by GO term, and the results showed that several DEGs identified were related to biological processes including positive regulation of the biological process and biological process. The downregulated NmrA-like and FKBP_C genes might be benefit to the growth of mycelia. Those results can explain that exiguous IAA and IBA improved the growth of A. gallica to some extent. We speculate that IAA and IBA are signaling molecules, and regulate the expression of growth-related genes of A. gallica 012 m by the same signaling pathway.     

Poly (triethylenglycol monomethacrylate) and poly(glycerol monomethacrylate) cross-linked gel as potential viscoelastics for intraoperative use

Background: The highly swelling poly(glycerol monomethacrylate) gel (polyGLYMA) and hydrophilic polymer poly(triethylenglycol monomethacrylate (polyTEGMA) were tested as potential viscoelastics for intraopertive use in anterior segment surgery. Methods: PolyGLYMA was implanted into the anterior chamber in 5 rabbits, and 40% polyTEGMA in 16 rabbits. The eyes were enucleated 1 week to 3 months after the operation. The corneal endothelium was examined with specular microscopy, and then the whole eye histopathologically. Results: In all eyes of the polyGLYMA group, the clinical findings were characterized by a marked ciliary injection and severe secondary glaucoma, and the histologic ones by a marked inflammatory infiltration and thickening of Descemet's membrane in the anterior chamber angle. Specular microscopy revealed a decrease in the endothelial cell density and polymorphism of the endothelial cells. In the polyTEGMA group, the anterior segment and the fundus were physiologic all the time, and specular microscopy and histologic findings showed no degenerative and/or inflammatory changes. Conclusions: PolyGLYMA proved unsuitable for intracameral application in rabbits. The new polymer polyTEGMA is characterized by high biologic tolerance after its implantation into the anterior chamber of rabbits. PolyTEGMA 40% might be considered as a potential viscoelastic material in humans.