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991.
目的编制一份测量和谐完美主义者合理认知与行为的量表。方法在理论分析与访谈的基础上,结合中国青少年、大学生完美主义量表的项目进行数据搜集和管理,运用内容分析、残差规整以及因素分析最终确定和谐完美主义的项目与维度,并进行了信度、效度检验。结果和谐完美主义心理由合理信念、自我提升、完美动机、自省、悦纳他人5个维度构成,各维度的内部一致性系数为0.75~0.88,重测信度为0.71~0.86,累积方差贡献率为61.57%。结论和谐完美主义量表具有较理想的信效度,较适合本土学生应用。  相似文献   
992.
目的 鉴定一个人类白细胞抗原(human leukocyte antigen,HLA)新等位基因HLA-B*4609.方法 使用序列特异性寡核苷酸PCR技术进行HLA基因分型,发现反应格局异常的可疑新等位基因,应用分子克隆和DNA双向测序技术测定新等位基因的核苷酸序列,并与已知等位基因进行序列比对分析.结果 检出1个样本HLA-B位点反应格局异常,DNA测序分型结果一个为B*151101,另一个的核苷酸序列与已知的HLA等位基因均不同,该基因序列与同源性最高的HLA-B*460101基因序列相比,在第3外显子区域中527位碱基发生A→T突变,导致176位编码氨基酸由谷氨酸(GAG)变成缬氨酸(GTG).结论 样本中含有HLA-B新等位基因序列.该序列申报后,被世界卫生组织HLA因子命名委员会正式命名为HLA-B*4609.  相似文献   
993.
职业紧张与冠心病的病例对照研究   总被引:12,自引:0,他引:12  
目的 :(1)探讨男性列车乘务人员职业紧张与冠心病发病的关系。方法 :应用职业紧张因素测试量表 (OSI) ,对男性列车乘务人员中 3 9例冠心病患者进行 1:2的配比病例对照研究并进行多因素条件Logistic回归分析。结果 :(1)在控制和考虑了非职业紧张因素条件下 ,职业紧张与冠心病关系密切 (OR =2 18,95 %CI :1 15 -4 15 ) ,而且随着职业紧张程度的增加 ,患冠心病的危险性显著增加 ;(2 )以铁路乘务人员为对照时 (与病例同工种 ) ,职业紧张仍与冠心病发病有关联 (OR =4 43 ,95 %CI :1 60 -12 2 5 ) ,但其OR值明显低于以其他服务行业人员为对照时的OR值 (OR =9 75 ,95 %CI:3 18-2 9 92 )。结论 :职业紧张是引起男性列车乘务人员冠心病发病的重要危险因素之一。  相似文献   
994.
Chlamydiae are a major cause of infertility and preventable blindness and there is currently no effective vaccine in humans or rodents against these organisms. We have previously shown that a peptide of 12 amino acids (termed TINKP) from a conserved region of the major outer membrane protein (MOMP) of Chlamydia trachomatis (C. trachomatis) is a primary T-cell epitope in humans. Here we showed that when dendritic cells (DC) from C3H or BALB/c mice were pulsed in vitro with the peptide they stimulated proliferation of syngeneic T cells in vitro indicating that the peptide is also a primary T-cell epitope in mice. Since the skin is a rich source of DC, we immunized mice from each strain with an intradermal injection of the peptide. Humoral and cell-mediated immunity to peptide, MOMP or whole elementary bodies (EB) of C. trachomatis (F/NI1/GU) were assessed. No antibody response to TINKP was observed. However, immunized mice showed recall responses to all three chlamydial antigens. T-cell-mediated immunity in the absence of antibody was induced by a single injection of the peptide intradermally. C. trachomatis isolated from the human genital tract causes salpingitis in mice. Preliminary studies in susceptible C3H mice indicated that intradermal injection of peptide conferred some protection against the development of salpingitis. Thus, a primary T-cell epitope identified by in vitro stimulation using DC can also initiate cell-mediated immunity in vivo and this approach may be useful in the development of vaccines.  相似文献   
995.
我们试用标记分析法,用3种单克隆抗体(黄病毒科特异性、登革病毒属特异性和登革病毒2型型特异性单克隆抗体)研究了我国海南省1985~1987年3年内流行的登革2型病毒8个流行株的抗原性变化,并与标准株新几内亚B株进行了比较。发现6株与标准株类似,2株显示出明显的差异。标记分析法为病毒抗原分析提供了一个简便、快速的方法,可用来监测一个地区病毒株群的变化及新株的传入。  相似文献   
996.
Mo XM  Xu CY  Kotaki M  Ramakrishna S 《Biomaterials》2004,25(10):1883-1890
Poly(L-lactide-co-epsilon-caprolactone) [P(LLA-CL)] with L-lactide to epsilon-caprolactone ratio of 75 to 25 has been electrospun into nanofibers. The relationship between electrospinning parameters and fiber diameter has been investigated. The fiber diameter decreased with decreasing polymer concentration and with increasing electrospinning voltage. The X-ray diffractometer and differential scanning colorimeter results suggested that the electrospun nanofibers developed highly oriented structure in CL-unit sequences during the electrospinning process. The biocompatibility of the nanofiber scaffold has been investigated by culturing cells on the nanofiber scaffold. Both smooth muscle cell and endothelial cell adhered and proliferated well on the P(LLA-CL) nanofiber scaffolds.  相似文献   
997.
We studied the identity and function of the 528-bp gene immediately upstream of Legionella pneumophila F2310 ptsP (enzyme I(Ntr)). This gene, nudA, encoded for a Nudix hydrolase based on the inferred protein sequence. NudA had hydrolytic activity typical of other Nudix hydrolases, such as Escherichia coli YgdP, in that Ap(n)A's, in particular diadenosine pentaphosphate (Ap(5)A), were the preferred substrates. NudA hydrolyzed Ap(5)A to ATP plus ADP. Both ptsP and nudA were cotranscribed. Bacterial two-hybrid analysis showed no PtsP-NudA interactions. Gene nudA was present in 19 of 20 different L. pneumophila strains tested and in 5 of 10 different Legionella spp. other than L. pneumophila. An in-frame nudA mutation was made in L. pneumophila F2310 to determine the phenotype. The nudA mutant was an auxotroph that grew slowly in liquid and on solid media and had a smaller colony size than its parent. In addition, the mutant was more salt resistant than its parent and grew very poorly at 25 degrees C; all of these characteristics, as well as auxotrophy and slow-growth rate, were reversed by transcomplementation with nudA. The nudA mutant was outcompeted by about fourfold by the parent in competition studies in macrophages; transcomplementation almost completely restored this defect. Competition studies in guinea pigs with L. pneumophila pneumonia showed that the nudA mutant was outcompeted by its parent in both lung and spleen. NudA is of major importance for resisting stress in L. pneumophila and is a virulence factor.  相似文献   
998.
绵羊肥大细胞中类胰蛋白酶的证实   总被引:20,自引:0,他引:20  
江萍  许乐仁 《解剖学报》1996,27(1):92-95
用甲苯胺蓝和阿尔辛蓝常规组织化学方法及特异性酶底物鉴定人肥大细胞类胰蛋白酶的酶组织化学技术,采用小鼠抗人肥大细胞类胰蛋白酶单克隆抗体,(AA1、AA3和AA5)通过间接免疫过的经物酶技术对绵羊肥大细胞的组织化学特性进行研究,酶组织化学技术及免疫组织化学技术均首次证实了绵羊肥大细胞颗粒中含有类胰蛋白酶,且该酶可作为绵羊肥大细胞的特异性标志,对于绵羊肥大细胞的常规组织化学染色,Carnoy液及中性缓门  相似文献   
999.
In previous studies, an in vitro ADP-ribosylation assay was developed to quantitatively evaluate the in vivo ADP-ribosylation of eukaryotic target proteins in intact Chinese hamster ovary (CHO) cells by pertussis toxin (PT). Immunoblot analysis identified the two PT-sensitive target proteins in CHO cells as Gi-2 and Gi-3. In this in vitro ADP-ribosylation assay, the ability of PT and ADP-ribosylate Gi-2 and Gi-3 intact CHO cells was not inhibited by cytochalasin D but was inhibited by chloroquine, monensin, and nocodazole. These data implicated the involvement of a cytochalasin D-independent endocytic mechanism, a pH-sensitive step, and microtubules in the ADP-ribosylation of Gi-2 and Gi-3 by PT in intact CHO cells. Preincubation of CHO cells with cycloheximide, at concentrations that reduced protein synthesis by > 95%, did not inhibit the ability of PT to ADP-ribosylate Gi-2 and Gi-3. Control experiments showed that these agents did not affect either the ability of PT to directly ADP-ribosylate the heterotrimeric G protein, Gt, or the binding of PT to CHO cells, except that monensin slightly inhibited the binding of PT to CHO cells. These results are consistent with a model in which PT is internalized by receptor-mediated endocytosis, probably via a cytochalasin D-independent pathway, which involves intracellular trafficking through late endosomes and the Golgi apparatus. An alternative model predicts the presence of a eukaryotic factor that traffics within cells via this pathway and is required by PT to ADP-ribosylate Gi proteins.  相似文献   
1000.
目的 观察休克期大面积切痂对严重烧伤大鼠细胞免疫功能的影响,探索改善烧伤后机体免疫功能紊乱的有效方法。方法 将大鼠分成休克期切痂组(A组)、常规切痂组(B组)和正常对照组(C组)。A、B组造成30%TBSAⅢ度烫伤,C组不烫伤。A组伤后第6h、B组伤后第4d切痂,并于伤后第1、5、9d各活杀10只,取材送检,观察其免疫指标的变化。结果 (1)A、B组与C组比较:A、B组烫伤大鼠各时相点CD3^+T细胞变化不大(P〉0.05),但CD4^+T细胞、CD4^+/CD8^+比值明显下降、CD8^+T细胞增高(P〈0.05或P〈0.01)。NK细胞活性明显下降(P〈0.05或P〈0.01),外周血CD25^+T淋巴细胞表达及经活化后脾脏CD25^+T淋巴细胞表达明显下降(P〈0.05或P〈0.01)。(2)A组与B组比较:A组CD4^+T细胞、CD4^+/CD8^+比值明显升高、CD8^+T细胞降低(P〈0.05或P〈0.01),NK细胞活性明显升高(P〈0.05或P〈0.01),外周血CD25^+T淋巴细胞表达及经活化后脾脏CD25^+T淋巴细胞表达均明显升高(P〈0.05或P〈0.01)。结论 (1)大鼠烫伤后细胞免疫状况发生了明显变化。(2)休克期切痂可以改善烫伤大鼠T淋巴细胞亚群分布,提高NK细胞活性,增加外周血CD25^+T淋巴细胞的表达。提高经活化后脾脏CD25^+T淋巴细胞数。从而改善烫伤大鼠伤后机体的细胞免疫功能。  相似文献   
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