盐酸环丙沙星水分的双溶剂费休氏法测定

采用以甲醇为基本溶剂,水为辅助溶剂的双溶剂费休氏法,可准确测定盐酸环丙沙星中分子内和游离状态的全部水分。其平均回收率为１００．０５％,ＲＳＤ为０．２５％,为溶解特性类似的药品提供了可靠的水分测定。     

支持性心理治疗对鼻咽癌患者抑郁情绪的干预研究

目的探讨支持性心理治疗对鼻咽癌患者的抑郁情绪的干预效果及其对生活质量和对同步放化疗依从性的影响。方法将96例鼻咽癌患者随机分为支持性心理治疗组和对照组,评定并比较两组SDS,SF-36生活质量得分,完成同步放化疗的比率。结果支持性心理治疗组SDS评分显著低于对照组（P〈0.01）。生活质量除生理机能外,其他指标支持性心理治疗组均优于对照组（P〈0.05或P〈0.01）。支持性心理治疗组显示良好的对同步放化疗治疗的依从性。结论支持性心理治疗能显著降低鼻咽癌患者抑郁情绪的严重程度,并可提高其生活质量,帮助患者完成同步放化疗治疗。     

胰头肿块型慢性胰腺炎12例诊治分析

目的分析胰头肿块型胰腺炎的诊治方法。方法回顾性分析我院1998年6月至2008年6月间收治的125例慢性胰腺炎病例,总结其中12例因胰头占位而行胰十二指肠切除术患者的病例。结果12例患者术中探查均显示肿块位于胰头,与周围组织有粘连,其中1例与门静脉有粘连,但尚能剥离,术后病理证实均为慢性胰腺炎。结论目前区分慢性胰腺炎与胰腺癌尚无特异性好和敏感性高的鉴别方法,CT结合经内镜逆行胰胆管造影对大部分病例的鉴别诊断具有较高的价值,胰头肿块型胰腺炎行胰头十二指肠切除术治疗效果良好。     

Localization of extracellular proteins of the human trabecular meshwork by indirect immunofluorescence

We used monospecific antibodies on semithin frozen sections to identify and localize the major tissue constituents of the nonglaucomatous human trabecular meshwork. The trabecular beams (sheets and cords) consist of a basement membrane (subendothelial extracellular matrix) surrounding an interstitial central core of connective tissue (substantia propria). The basement membrane contains collagen types III, IV, and V, the glycoproteins laminin and fibronectin, and the basement membrane-associated heparan sulfate proteoglycan. The trabecular basement membrane is unlike most subendothelial basement membranes because it contains collagen type III and a relatively disorganized structure. The central core contains collagen types I and III, and elastin. The closely linked juxtacanalicular meshwork contains collagen type III, but no collagen type I or elastin. The connective tissue composition of the trabecular meshwork appears similar to other highly compliant and resilient tissues, such as lung, blood vessels, and conjunctiva.     

连续环形撕囊术抑制后囊膜混浊的实验研究

目的：搪塞连续环形撕囊术（ＣＣＣ）对白内障囊外摘除术后晶体后囊膜混浊的影响。方法：将２４只白色家兔随机平均分为三个时间组,每只家兔又按双眼术中前切开法的不同随机分为ＣＣＣ组和开罐式截囊组,术后不同时期观察后囊膜病理变化。结果：术后一月,Ａ组后囊膜纤维增殖明显减少,赤道 后囊膜间无显著粘连,早期即在可在后囊膜面查见纤维弱细胞增生。结论：ＣＣＣ可抑制白内障术后晶体后囊膜混浊的发生。     

Postprocedural Outcomes of Rural Children Undergoing Correction of Congenital Heart Lesions in Yunnan Province, China

The management of congenital heart disease (CHD) remains a significant challenge in developing regions. Since 2006, China California Heart Watch has provided cardiac services in China’s Yunnan province. Our Grants for Kids program aims to diagnose and fund surgical and nonsurgical treatments for underprivileged children with congenitally malformed hearts. This report analyzes our patient outcomes. From 2007 to 2010, 36 children with CHD underwent either surgical or percutaneous procedures at local Chinese medical centers, and 94% of our patients could be contacted for follow-up assessment. The mortality and complication rates of our patient population compare favorably with international data. Our study provides a model through which networking with local hospitals and regional cardiac centers can be an effective way to assist developing areas in providing cardiac care to rural underserved populations.     

卡托普利防治腹主动脉狭窄性高血压大鼠心肌肥厚的形态定量研究

方法选用腹主动脉狭窄的大鼠实验性心肌肥厚模型,采用光镜、电镜形态计量手段,定量观察了血管紧张素转换酶抑制剂卡托普利防治心肌肥厚的作用,并比较了倍他乐克对心肌肥厚的作用。结果卡托普利组大鼠的全心／体重比、心肌组织／间质比、心肌细胞横径及心肌细胞线粒体体密度与对照组类似（p＞0．05）,明显不同于心肌肥厚组（p＜0．05）,而倍他乐克组上述多指标与心肌肥厚组相似（p＞0．05）。本实验表明卡托普利通过保护心肌细胞及间质而使心肌组织免遭损伤因素刺激发生肥厚的作用,即对大鼠高血压性。心肌肥厚具有良好的防治作用。     

Glycation inhibitory activity and the identification of an active compound in Plantago asiatica extract

The glycation reaction involves a series of non-enzymatic reactions between the carbonyl group on reducing sugars and the amino group on proteins leading to the formation of advanced glycation end-products (AGEs), which are acknowledged to be involved in the pathogenesis of diabetic and aging-related complications. Consequently, the development of AGE inhibitors is considered to have therapeutic potential in patients with diabetes or age-related diseases. The preliminary results showed that a methanol extract (PAE) of Plantago asiatica, which is traditionally used as a folk medicine in Asian countries to treat fever, cough, wound etc., had strong glycation inhibitory activity. The effects of the extract on AGE fluorescence were dose-dependent, reaching 41% inhibition at 0.1 microg/mL of extract. The purified principle from PAE was identified as plantamajoside. As well as antioxidant activities, in vitro glycation inhibitory activities with 10 and 25 mm plantamajoside were higher than those with 10 and 25 mm aminoguanidine. The results demonstrate that PAE and plantamajoside had significant effects on in vitro AGE formation, and the glycation inhibitory activity and antioxidant activity of plantamajoside were comparable to those obtained using millimolar concentrations of the standard antiglycation agent aminoguanidine, and the antioxidant ascorbate, respectively.