Immunoglobulin G is present in a wide variety of soft tissue tumors and correlates well with proliferation markers and tumor grades

BACKGROUND:

The traditional view that immunoglobulin (Ig) is produced only by B lymphocytes has been challenged, because it has been demonstrated that Ig genes and proteins are expressed in epithelial cancer cells. However, whether Ig expression in nonlymphoid cells is limited to epithelial cells is unclear. Because sarcomas differ distinctly from carcinomas in their biologic and clinical features, the authors investigated the question of nonlymphoid IgG expression in soft tissue lesions.

METHODS:

Immunohistochemistry, in situ hybridization, and polymerase chain reaction (PCR) were used to demonstrate IgG expression in 80 soft tissue lesions. The correlation between Ig expression and proliferation markers (proliferating cell nuclear antigen [PCNA], Ki‐67, and cyclin D1) in sarcomas was investigated by immunohistochemical and statistical analyses.

RESULTS:

Igκ was identified in 97.4% of sarcomas and in 31.7% of benign lesions by immunohistochemistry. The difference was statistically significant (P < .01). Messenger RNA from the IgG1 heavy‐chain constant region was also detected by in situ hybridization. Variable‐diversity‐joining recombination sequences of both heavy and light chains were obtained by PCR and sequencing. Moreover, the labeling index of PCNA, Ki‐67, and cyclin D1 was much higher in sarcomas with high Igκ expression than in sarcomas with low Igκ expression (P < .01 for PCNA and cyclin D1; P < .001 for Ki‐67). There were more grade 3 sarcomas with high Igκ expression compared with grade 1 and 2 sarcomas (P < .05).

CONCLUSIONS:

IgG was identified in a wide variety of soft tissue tumors and correlated well with proliferation markers and tumor grades. IgG may be a useful marker for cell proliferation in sarcomas. Cancer 2010. © 2010 American Cancer Society.     

Primary central nervous system Burkitt lymphoma as concomitant lesions in the third and the left ventricles: a case study and literature review

This study reports the case of a 75-year-old woman with intermittent speech disturbance, headache, and general malaise. Brain magnetic resonance imaging showed a double lesion located in the third ventricle and temporal horn of the lateral ventricle. We completely removed the lesion in the third ventricle, adhering to the septum pellucidum. The histopathological diagnosis was Burkitt lymphoma, which was confirmed to be primary in postoperative investigation. To our knowledge, this is the first case of primary Burkitt lymphoma involving cerebral ventricles. Finally, we reviewed the characteristics of primary central nervous system Burkitt lymphoma.     

广西自治区听力残疾人抽样调查结果分析

目的根据广西自治区第二次全国残疾人抽样调查的资料,调查分析广西自治区听力残疾人的数量、地区分布、致残原因等。方法采用分层、多阶段、整群、概率比例抽样方法进行残疾人口调查。全自治区共抽查25个县（市、区）、100个乡（镇、街道）,共200个调查小区。每个调查小区常住人口约400人,调查总户数22569户,实际调查量为80712人,调查的抽样比1．73％。应用数字式便携听力计对每位调查对象进行听力检测,问卷调查病史,专科医师体检并作出病因诊断。结果5837名残疾人中,听力残疾1467人,占总残疾人数的25．13％,居所有残疾之首;其中一级听力残疾70人（4．77％,70／1467）;二级听力残疾127人（8．66％,127／1467）;三级听力残疾689人（46．97％,689／1467）;四级听力残疾581人（39．60％,581／1467）。主要致残原因：老年性聋911人（62．10％,911／1467）;原因不明155人（10．57％,155／1467）;耳疾158人（10．77％,158／1467）;全身性疾病111人（7．57％,111／1467）;其他132人（8．99％,132／1467）。男性821人（56％）,女性646人（44％）,性别比为1．27：1。农村居民910人（62％）,城市居民557人（38％）。年龄分布：0～岁3人（O．20％）,7～岁10人（0．68％）,18～岁215人（14．66％）,≥60岁1239人（84．46％）。结论听力残疾在广西所有残疾人口中发生率最高,以中老年性聋和耳疾占大多数。     

Diagnostic value of glucose-6-phosphate isomerase in rheumatoid arthritis

BackgroundAlthough glucose-6-phosphate isomerase (G6PI), anti-G6PI antibodies and G6PI-containing immune complexes (G6PI-CIC) have proved high expression in patients with rheumatoid arthritis (RA), comprehensive evaluation of the G6PI-derived markers, G6PI antigen, anti-G6PI Abs, G6PI-CIC and G6PI mRNA, in the diagnosis of RA remains necessary.MethodsWe measured G6PI antigen, anti-G6PI Abs, C1q/G6PI-CIC as well as anti-cyclic citrullinated peptide antibodies (anti-CCP Abs) in serum and concomitantly synovial fluid (SF) by ELISA in RA, other rheumatic diseases and healthy controls. The G6PI mRNA expression in peripheral blood mononuclear cells (PBMCs) was assessed with real-time PCR.ResultsAs compared with non-RA patients, RA patients had increased levels of G6PI antigen, anti-G6PI Abs, C1q/G6PI-CIC and G6PI mRNA expression in sera or PBMCs, and increased levels of G6PI and C1q/G6PI-CIC in SF. The serum G6PI levels in RA patients positively correlated with anti-G6PI Abs, C1q/G6PI-CIC, G6PI mRNA, anti-CCP Abs, RF, CRP and ESR, respectively. The area under curve analyses demonstrated that serum G6PI had the best discriminating power for RA and active RA followed by C1q/G6PI-CIC, anti-G6PI Abs and G6PI mRNA. The simultaneous use of serum G6PI and anti-CCP Abs assays in the form of either of them tested positive gave improved sensitivities of 88.1% for RA and 95.8% for active RA.ConclusionsDespite the elevated expression of all G6PI-derived markers in RA, the serum G6PI has the best discriminating power among the four G6PI-derived markers. The serum G6PI determination either alone or in combination with anti-CCP Abs improves the diagnosis of RA.     

Identification and validation of specific methylation profile in bile for differential diagnosis of malignant biliary stricture

ObjectiveThis study was aimed to identify the specific methylation profile in bile specimens of pancreaticobillary diseases for differential diagnosis of malignant biliary stricture.Design and methodsIn a total of 80 bile specimens from pancreaticobillary diseases, the methylation status of 19 tumor suppressor genes were analyzed by methylation-specific PCR and the methylation index (MI) were compared between the malignant and benign groups.ResultsMethylation of DKK3, p16, SFRP2, DKK2, NPTX2 and ppENK were more frequently detected in the bile of malignant biliary strictures than benign patients. When setting MI 0.5 as the threshold, this 6-gene panel could distinguish the malignant biliary stricture with a high sensitivity, specificity and accuracy (77.27%, 77.78% and 77.50%, respectively).ConclusionThe methylation profile including 6 specific genes in bile may be a promising biomarker for differential diagnosis between malignant and benign biliary strictures.     

CUL5基因沉默对连续照射期间CNE2细胞增殖状况影响的研究

背景与目的:本课题组前期已应用不同的实验方法对鼻咽低分化鳞癌细胞株(CNE2)在连续分割照射中的细胞增殖状态进行了研究,明确了CNE2细胞株在连续分割照射中存在中后期加速再增殖现象,同时应用基因芯片技术、实时荧光定量PCR筛选出与照射后CNE2细胞株增殖状态相关的差异基因.CUL5基因为细胞增殖活性增高时的上调基因之一,其在照射增殖活性最高时相与最低时相表达差异在2倍以上,因此本研究选择CUL5作为开展功能研究的目的基因,应用RNA干扰技术沉默CUL5基因,观察人鼻咽癌细胞株CNE2在连续照射中后期加速增殖现象是否受到影响.方法:构建、培养出CUL5基因沉默效果稳定的人鼻咽癌细胞株CNE2-pGPU6/GFP/Neo-CUL5,接受射线2 Gy/d,每天1次,连续照射5 d,每天分别用四唑盐比色法(MTT法)检测细胞相对增殖率、流式细胞术检测照射后细胞周期分布情况.结果:MTT法检测出照射第1～5天细胞相对增殖率,实验组(CNE2-pGPU6/GFP/Neo-CUL5)分别为1.03±0.05、0.97±0.03、0.84±0.12、0.54±0.09、0.33±0.11,阴性对照组(CNE2-pGPU6/GFP/Neo-NC)分别为1.07±0.09、0.96±0.1 2、1.21±0.04、1.19±0.04、0.91±0.02;空白对照组(CNE2)分别为1.07±0.09、0.97±0.05、1.22±0.07、1.22±0.04、0.97±0.04,实验组与阴性及空白对照组均有显著差异(P＜0.001).流式细胞术检测出实验组在连续照射第1天增殖速度最快,增殖最旺盛,后逐渐下降,第5天增殖最慢,增殖活性最低;对照组第3天增殖速度最快,第5天增殖最慢.结论:CUL5基因沉默的CNE2细胞连续分割照射期间加速增殖现象有所改变.     

子宫内膜间质肉瘤:一例在不孕症治疗中诊断的病例(英文)

The patient with irregular menstruation went to our Department (Gynecological Department, the Third AffiliatedHospital, Sun Yat-sen University, Guangzhou, China) with the complaint of no pregnancy at the age of 26 in 2007, and oralcontraceptive pills were given when simple type of endometrial hyperplasia was pathologically diagnosed, and then ovulationinduction was performed. But after nearly two years from the beginning of infertility treatment, endometrial stromal sarcomawas diagnosed and radical treatment was performed for the young patient. And the relative relationship between endometrialstromal sarcoma and infertility is worth discussing.     

Variation of cataract surgery costs in four different graded providers of China

Background  

China has the largest population of cataract patients in the world. However, the cataract surgery rate per million remains low in China. We carried out a survey on costs of cataract surgery from four different graded providers in China and analyzed differences in cost among these clinics.     

横腭弓与Nance弓联合增强支抗的研究

目的：评价临床拔牙矫治病例使用Nance弓和横腭弓联合支抗的控制效果。方法：选择11~28岁30例严重前牙拥挤或上颌、双颌前突错颌患者使用Nance弓与腭弓联合支抗,分析治疗前后X线头影测量值以及牙颌研究模型的各项指标变化。结果：磨牙前移量未达到拔牙间隙的1/3;∠G。Gn-SN和ALFH/AFH无显著变化,6 6宽度矫治前后无显著差异。结论：Nance弓与横腭弓联合支抗是一种简单有效的装置,具有三维支抗的作用,能够提供中到重度支抗,且对磨牙伸长进行较为有效的限制。