左旋四氢巴马汀对单个豚鼠心室肌细胞钙电流的阻滞作用

AIM: To study the effect of l-tetrahydropalmatine (l-THP) on L-type calcium channel. METHODS: Patch clamp technique (whole cell recording) was used to record L-Ca2+ current in single cardiac myocyte. RESULTS: 1) l-THP 1, 10, and 100 micromol.L-1 reduced ICa-max from (999 +/- 93) pA to (700 +/- 111) pA, (582 +/- 66) pA, and (420 +/- 112) pA (n = 6, P < 0.01), respectively. 2) l-THP reduced the voltage at half-maximal inactivation (V1/2) of L-Ca2+ channel to more negative potentials by 9 mV (n = 5, P < 0.05). 3) l-THP caused both tonic and use-dependent reduction of Ca2+ current. Tonic block of l-THP on Ca2+ current was 46% +/- 8% (n = 6, P < 0.01). The degree of use dependent blocking was 13.5% +/- 2.4% (n = 6, P < 0.05) at 1 Hz, the degree increased to 44% +/- 5% (n = 6, P < 0.01) at 3 Hz. 4) l-THP delayed half-recovery time of Ca2+ channel recovery from inactivity from (94 +/- 39) ms to (170 +/- 42) ms(n = 6, P < 0.01). CONCLUSION: l-THP has a moderate inhibitory effect on L-Ca2+ current.     

The von-Hippel-Lindau (VHL) tumor suppressor gene is not mutated in sporadic ovarian carcinomas

The von Hippel-Lindau (VHL) tumor suppressor gene has been shown to be mutated frequently not only in neoplasms from von Hippel-Lindau disease, but also in sporadic clear cell renal carcinoma. In order to reveal the possible role of the VHL tumor suppressor gene in the development of ovarian carcinoma, a total of 71 primary sporadic ovarian carcinomas were analyzed for the presence of mutations in the exon 2 and 3 of the VHL tumor suppressor gene, using the polymerase chain reaction with single strand conformation polymorphism analysis. No mutations in the VHL gene were found in any of the tumors analyzed. This result shows that the VHL tumor suppressor gene does not play a major role in the tumorigenesis of sporadic ovarian carcinoma.     

中麻催醒剂——Ⅰ.二甲氨基甲酸间-(烷氨基)烷氧基苯酯的合成

合成了一系列二甲氨基甲酸间-(烷氨基)烷氧基苯酯,并进行了实验动物的毒性和中麻催醒试验。初步临床观察表明,化合物Ⅱ₄(现命名为催醒安)比毒扁豆碱的催醒效果好,而毒性小。简要地讨论了结构和生物活性之间的关系。     

"气至病所"针法治疗臀上皮神经损伤的疗效观察

目的：观察气至病所针法的临床治疗效果。方法：将120例臀上皮神经损伤病例随机分成“气至病所”针法组与常规针法组进行对照观察。结果：气至病所组治愈率优于常规组(P＜0．01)。结论：“气至病所”针法是一种治疗臀上皮神经损伤的有效方法。     

Use of YAG laser combined with mucosal suturing for the treatment of polypoid vocal cord]

To improve low-pitched voices in cases with polypoid vocal cords, YAG laser irradiation combined with a mucosal suturing technique was attempted in 9 female cases with severe polypoid changes in their vocal cords. A YAG laser beam (5 to 10 W) was used to irradiate the upper surface of the polypoid vocal cord. The polypoid content of the cord was gradually coagulated, and the free edge of the cord appeared to slide up toward the burned area. The polypoid content was then removed and squeezed through an open wound made in the burned area using a conventional method. Bleeding was successfully controlled using the laser. After the excessive mucosal margin was trimmed and the contour of the vocal cord was adjusted, the wound was closed by 7-0 monofilament absorbable suture. Suturing was relatively easy because the mucosal edge was also coagulated. Postoperative evaluations of voice quality revealed an improvement in the GRBAS scale of voice quality as well as an elevation in voice pitch and an upwards shift in the voice range in all cases.     

神阙穴敷贴对原发性骨质疏松症骨钙素的影响

目的　观察神阙穴贴补血益精透皮贴对原发性骨质疏松症骨钙素 (BGP)的影响。方法　选择原发性骨质疏松症患者130名 ,分神阙穴贴药组 (补血益精穴位透皮贴剂 )、西药组 (羟乙磷酸钠片 )、中药组 (补血益精药丸 )、空白对照组。结果和结论　穴位敷贴组能显著提高骨钙素 (7.69± 1.65 )。穴位敷贴组与中药组 (7.82± 0 .99)和西药对照组 (7.13± 0 .89)对原发性骨质疏松症的骨钙素调整作用基本一致 (P>0 .0 5 )。     

抗柯萨奇B中药胶囊治疗病毒性心肌炎的临床疗效评价

目的观察抗柯萨奇B心肌炎胶囊(ACCM)治疗病毒性心肌炎(VM)的临床疗效.方法将120例VM患者随机分成ACCM组(用ACCM治疗,60例)和对照组(西药常规治疗,60例),进行对比观察.结果ACCM组总有效率85%,明显优于对照组的57%(P<0.05).治疗后ACCM组的心悸、反复感冒、胸闷、胸痛、气短、乏力、头晕等症状的恢复明显优于对照组.结论ACCM是治疗VM的一种高效、价廉及使用方便的中药制剂, 无明显不良反应.     

p27kip1及p53基因蛋白与恶性卵巢上皮性肿瘤预后的关系

目的　探讨 p2 7kip1和 p5 3基因蛋白在恶性卵巢上皮性肿瘤中的表达及其与预后的关系。 方法　1992年 11月至 1999年 12月应用免疫组化检测 5 6例恶性卵巢上皮性肿瘤石蜡切片中 p2 7kip1和p5 3基因蛋白表达情况 ,并应用Kaplan -Mier法及多变量Cox比例风险回归模型分析患者预后。 结果　 5 6例恶性卵巢上皮性肿瘤患者的 p2 7kip1基因蛋白表达率为 4 1 1% ;p5 3基因蛋白表达率为 4 8 2 % ,两种基因蛋白表达比较 ,差异无显著性 (P >0 0 5 )。p2 7kip1及 p5 3基因蛋白表达与肿瘤的病理分级有关 ( P <0 0 5 ) ,与其他临床病理特征无关。单因素分析 :p2 7kip1及 p5 3基因蛋白表达与患者的中位生存时间、生存曲线各时点生存率密切相关。 结论　p2 7kip1基因蛋白表达对恶性卵巢上皮性肿瘤的预后有显著影响 ,而 p5 3基因蛋白表达与预后关系不显著     

外阴癌中HPV感染与p53、MDM2表达的关系

目的 :探讨HPV6/ 11、16/ 18在外阴癌组织中的感染情况及与p5 3、MDM2蛋白表达的关系。方法 :用原位杂交法 (ISH)检测HPV6/ 11、16/ 18在 30例外阴癌、2 1例外阴上皮内瘤变 (VIN)及 10例外阴正常皮肤组织中的表达。同时用免疫组化SP法检测p5 3、MDM2蛋白的表达。结果 :HPV6/ 11、16/ 18在外阴癌、VIN中的阳性表达率分别为 60 % (18/30 )、33.33% (10 / 30 ) ,4 2 .86% (9/ 2 1)、2 8.5 7% (6/ 2 1) ,正常对照组没有表达。p5 3、MDM2蛋白在外阴癌、VIN、正常对照组中的阳性表达率分别为 63.33% (19/ 30 )、4 0 .0 0 % (12 / 30 ) ,4 7.62 %(10 / 2 1)、5 2 .38% (11/ 2 1) ,0 % (0 / 10 )、0 % (0 / 10 )。HPV6/ 11的表达在外阴癌组、VIN组与正常组差异有显著性 (P <0 .0 5 ) ,外阴癌组HPV16/ 18表达与正常组差异有显著性 (P <0 .0 5 )。外阴病变各组p5 3、MDM2与正常组差异有显著性 (P <0 .0 5 )。结论 :HPV6/ 11、HPV16/ 18、p5 3、MDM2蛋白在外阴组织的不同病变中表达均差异有显著性 ,在外阴癌的发生发展中HPV感染、p5 3突变和MDM2表达可能起一定的作用