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941.
Generation of dendritic cells in vitro from peripheral blood mononuclear cells with granulocyte-macrophage-colony-stimulating factor, interleukin-4, and tumor necrosis factor-alpha for use in cancer immunotherapy. 总被引:8,自引:0,他引:8
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OBJECTIVE: The purpose of the study was to characterize the requirements in terms of precursors, developmental pathways, and media for the generation of large numbers of mature dendritic cells (DC) under conditions acceptable for use in adjuvant, active immunotherapy strategies for surgically treated malignancies. SUMMARY BACKGROUND DATA: Although limited previously by the small numbers accessible, DC-based immunotherapies for malignancy have become more realistic with the development of methods for efficiently generating larger numbers of DC from peripheral blood mononuclear cells (PBMC) in vitro, but these methods rely on clinically unacceptable culture conditions (such as inclusion of fetal bovine serum), necessitating the development of methods for generating functionally equivalent DC in serum-free conditions. METHODS: Plastic-adherent PBMC (from healthy donors and patients with cancer) were incubated for 7 days with granulocyte-macrophage-colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) with and without tumor necrosis factor-alpha (TNF-alpha) in fetal bovine serum-containing and serum-free media and were analyzed by Wright's stain for morphology, flow cytometry for phenotype, and mixed lymphocyte reaction for allostimulatory function. RESULTS: Growth in either serum-containing or serum-free media supplemented with GM-CSF and IL-4 yielded a similarly heterogeneous population of cells, 6% to 10% of which had the morphology (large cells with thin projections), immunophenotype (including CD83+), and function of mature DC. Tumor necrosis factor-alpha significantly augmented the number of these mature DC, whereas preculture depletion of CD14+ PBMC virtually eliminated them. CONCLUSIONS: Generation of mature DC in the authors' serum-free clinically applicable conditions is similar to serum-containing conditions and requires CD14+ precursors, differentiation through a CD14-CD83- immature stage under the influence of GM-CSF and IL-4, and maturation into a CD83+ DC under the influence of TNF-alpha. 相似文献
942.
为了探讨彩色多普勒血流显像技术显示正常人睾丸内血流的可行性,并探讨睾丸的血流动力学特征,我们对20名正常人双侧睾丸进行了彩色多普勒检查。结果显示,彩色多普勒能显示所有睾丸的包膜动脉、向心动脉。正常睾丸的包膜动脉和向心动脉收缩期峰值血流速度的均值分别为9.00cm/s和7.42cm/s;舒张末期血流速度均值分别为3.50cm/s和3.38cm/3,本文对睾丸彩色血流显像和多普勒频谱特点也作了讨论。 相似文献
943.
急性胰腺炎与内皮素、一氧化氮和氧自由基关系研究 总被引:14,自引:1,他引:13
目的 研究急性胰腺炎 (AP)与内皮素 (ET)、一氧化氮 (NO)和氧自由基关系。方法 急性胰腺炎 71例 ,应用放射免疫分析法测定血浆ET、NO和超氧化物歧化酶 (SOD) ,并与健康体检者 15例作对照。结果 急性胰腺炎血浆ET、NO明显升高 ,而SOD明显降低 ,与健康对照组比较差异显著 (P <0 0 1) ;且重症胰腺炎的ET和NO增高、SOD降低尤为明显 ,与轻症胰腺炎相比较有统计学意义 (P <0 0 1)。结论 血浆的ET、NO和SOD异常消长在急性胰腺炎发生、发展过程中具有重要作用。 相似文献
944.
Zhang Gui-mei Hao Tian-ling Yang Yu-zhen Feng Zuo-hua Jiang Zhi-yao Zhou Bin Zhang Hui 《华中科技大学学报(医学英德文版)》1992,12(3):150-153
Summary Effects of Chinese Medicinal Preparation “Re Du Qing” (RDQ) on the activation, proliferation and membrane fluidity of T lymphocytes
from human peripheral blood Were studied by means of3H-TdR incorporation and DPH fluorescence polarization. The results showed that “RDQ” can:1) significantly inhibit the activation
of T lymphocytes; 2) restrain the proliferation of activated T lymphoblasts in the presence of exogenous interleukin-2 (IL-2);
and 3) increase the membrane fluidity of T lymphocytes and antagonize the decreased fluidity of lymphocyte membrane mediated
by Con A or PHA. The functional abnormalities of T lymphocytes in some autoimmune diseases such as arthritis and the usefulness
of RDQ in the treatment of these diseases were also discussed. 相似文献
945.
946.
Tongqiao Huoxue Tang and Buyang Huanwu Tang for Treatment of Vascular Dementia -A report of 36 Cases
To study the effects of Tongqiao Huoxue Tang (通窍活血汤) and Buyang Huanwu Tang (补阳还五汤) onvascular dementia (VaD), 36 VaD patients in thetreatment group were treated with the two decoctions,which were compared with 28 cases in a controlgroup treated with Duxil. 相似文献
947.
Effect of shenmai Injection on SIL-2R NK and LAK cells of patients with advanced carcinoma
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Serum interleukin-2 receptor (sIL-2R) level, activities of natural killer cell (NK) and lymphokine activated killer (LAK) cells were determined in 60 patients with advanced carcinoma (AC) before and after treatment with Shenmai Injection (SMI), forty healthy persons were taken as non-carcinoma control (NC). The results showed that: Serum sIL-2R level in AC were much higher than those in NC (P<0.05) and activities of NK and LAK cells in AC were much lower than those in NC (P<0.05) before treatment. There was no significant difference among gastric, colonic and lung cancer (P>0.05). After treatment with SMI, it was found that the level of sIL-2R in all patients were obviously lowered (P<0.05) while the activities of NK and LAK cells were signifficantly higher than that prior the treatment (P<0.05). Relevancy was not found between sIL-2R and NK, LAK cells. These data suggested that the immune function was compromised in AC, and SMI has a wide effect of immuno-regulation. 相似文献
948.
反映手术间利用效率的统计指标与方法探讨 总被引:1,自引:0,他引:1
目的 探索反映手术室工作状态的指标和方法,为了解科室需求、合理利用手术室资源奠定基础。方法 提取医院局域网中有关手术安排、手术过程、麻醉等信息,通过常用统计指标、统计图表,反映手术室、手术科室、手术间的工作状态。结果 工作日手术问日均工作6.34小时,节假日日均工作0.6l小时。工作日13点后有近l/3的空台,14点后近l/2空台。个别手术间利用率偏低。结论 1.由在用手术间时点统计结果可见,14点后接台手术尚有一定潜力,节假日安排手术潜力更大;2.工作日间均工作时数已近满负荷,若要进一步提高手术间的利用,需加强麻醉科和手术室力量。 相似文献
949.
刺梨汁对慢性氟中毒的影响及机理研究 总被引:1,自引:0,他引:1
用高氟饲料喂养大鼠6个月,复制出慢性氟中毒模型,再自由饮用刺梨汁3个月,探讨刺梨汁对慢性氟中毒的影响及机理。结果表明:刺梨汁可明显改善氟中毒的一般状况,对已形成的氟斑牙影响不大,但可促进尿氟排泄,降低血清和骨氟含量及尿羟脯氦酸含量,提高血清中维生素C、维生素E和血清、肝、肾GSH含量,增强血、肝、肾GSH-Px和SOD活性,降低血清、肝和肾LPO含量,降低尿γ-GT和血清GPT活性及肝TG含量,提示:刺梨汁具有明显桔抗慢性氟中毒作用,其机理可能是通过促进尿氟排泄和抗氧化作用。 相似文献
950.
J D Lee K H Shin S N Cho J S Shin M G Lee W I Yang C Y Park H S Yoo J T Lee O D Awh 《European journal of nuclear medicine》1992,19(12):1011-1015
Immunoscintigraphy with radiolabelled monoclonal antibodies is widely used to detect solid tumours, but only a few trials have been carried out concerning the specific in vivo localization of an inflammatory process. The purpose of this study was to investigate the detectability of tuberculous foci utilizing this method with radiolabelled bacillus Calmette-Guérin (BCG)-specific F(ab')2 in rabbits. All of the tuberculous lesions (n = 8) were clearly visualized on serial scintigraphy for up to 48 h after injection of the antibody. Immunohistochemical and Ziel-Neelson staining of the tuberculous lesions confirmed the presence of the tuberculous antigens and bacilli. It failed to demonstrate any sustained retention of the BCG-specific antibody fragment in the control group with syphilitic orchitis (n = 2). Therefore, the specific in vivo localization of tuberculosis is feasible by immunoscintigraphy. 相似文献