Gingival crevicular fluid levels of SLIT3 are increased in periodontal disease

This study aims to investigate the levels of SLIT3 in gingival crevicular fluid (GCF) of healthy and periodontal disease subjects, and their correlations to periodontal disease. A total of 45 periodontal patients and 45 periodontally healthy volunteers were enrolled. The clinical parameters, radiographic bone loss and the levels of SLIT3, receptor activator of NF‐κB ligand (RANKL) and osteoprotegerin (OPG) in GCF were measured. The prevalences of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia in subgingival plaque were also analyzed. The expression of SLIT3 and RANKL was detected in the periodontium of experimental periodontitis in rats and lipopolysaccharide (LPS)‐induced mouse macrophage. The total amounts and concentrations of SLIT3 and RANKL were significantly higher in periodontitis than those in healthy, while the level of OPG was significantly lower (p < .05). Significant positive correlations were observed between the level of GCF SLIT3 and clinical attachment level and radiographic bone loss (p < .05). There existed a significant positive correlation between SLIT3 and RANKL (p < .05). Increased expression of SLIT3 and RANKL was observed in the periodontium of periodontal rats. SLIT3 expression was induced by LPS stimulation in macrophages. These results suggest that SLIT3 may act as a diagnostic indicator of periodontal disease and should be further investigated.     

Intentional replantation of periodontally involved hopeless teeth: a case series study

To explore the clinical effect, the healing modes, and the potential influence factors of intentional replantation for periodontally hopeless teeth in comb     

Epidemiological,clinical, and 3-dimentional CBCT radiographic characterizations of supernumerary teeth in a non-syndromic adult population: a single-institutional study from 60,104 Chinese subjects

Objective

This study was aimed to delineate the prevalence, clinical, and 3-dimentional radiographic characteristics of adult supernumerary teeth (ST found) in a Chinese non-syndromic, dental population.

Materials and methods

Medical records and cone beam computed tomography (CBCT) images were utilized to identify adult patients with ST in a tertiary referral dental hospital between June 2012 and December 2018. CBCT scan coupled with 3-dimentional reconstruction was used to characterize the detailed location, morphology, orientation of ST, and their relationship with adjacent teeth and neighboring structures. All relevant information regarding age and gender of patients, morphology, and 3-dimentional topography of ST as well as ST-associated complications were recorded and statistically analyzed.

Results

A total number of 1149 ST was identified in 921 eligible patients screened from 60,104 subjects with the prevalence of 1.5%. Male patients outnumbered females with a gender ratio of 1.76:1. The majority of ST was single, located in the maxilla, especially the maxillary central incisor region. Most ST were conical shape, inverted orientation, and impacted. ST-associated complications including impaction or root resorption of adjacent teeth, and cystic/tumor-like lesions were totally found in 13% ST and significantly associated with location, orientation, and morphology of ST.

Conclusions

Most ST in Chinese adults were conical, inverted, impacted, and located in the maxillary central incisor region, and associated with various complications. Our findings offer valuable information concerning the prevalence, clinical, and radiographic characteristics of ST in non-syndromic Chinese adults.

Clinical relevance

These findings are beneficial for clinicians to comprehensively understand the incidence, pathogenesis, and clinical management of ST.

     

穿支皮瓣修复口腔颌面-头颈部缺损专家共识

穿支皮瓣由皮肤穿支血管供血,是常用的组织缺损修复形式,但头颈部解剖复杂、缺损形式多样以及穿支血管解剖变异等,阻碍了穿支皮瓣在口腔颌面-头颈部缺损修复中的进一步应用。迄今为止,国内外对于穿支皮瓣修复口腔颌面-头颈部缺损临床应用缺少相关治疗指南,穿支血管的术前评价缺乏统一标准,穿支皮瓣的应用设计缺乏科学的规范,穿支皮瓣修复术后缺少客观的监测和评价体系。为进一步统一和规范穿支皮瓣在口腔颌面-头颈部缺损修复中的应用,提高重建效果,本共识总结全国多家著名医学院校及附属医院口腔颌面外科专家团队的实践经验,供临床医师参考。     

网络平台在口腔颌面外科专科医师培训的初步应用

网络平台是口腔颌面外科专科培训的重要途径,具有极大容量的教学空间,且无时空地域限制,资源丰富。网络平台可打破传统上口腔颌面外科医师通过教材或书籍获取知识的局限,大大提高了获取信息医学资源的速度,为专科医师培训提供了一个更广阔、便捷的方式。因此,加速推广网络平台在口腔颌面外科专科医师培训的应用,有利于保障专科医师规范化培训工作的顺利实施,提高培训质量。     

整体切削的聚醚醚酮可摘局部义齿即刻修复1例

聚醚醚酮(PEEK)是一种具有良好的力学性能和生物安全性的新型高分子化合物,近年来逐渐应用于口腔修复、种植、正畸等领域。目前,PEEK在可摘局部义齿中的应用已有临床报道,但整体切削的PEEK可摘局部义齿还未见相关临床报道。本病例应用全程数字化设计和PEEK材料,整体切削制作了集支架、人工牙和基托为一体的可摘局部义齿,对1例83岁上下颌牙列缺损的患者进行了即刻修复治疗,并进行了3~6个月的随访,患者对修复效果满意。     

药物相关性颌骨坏死治疗方法的研究进展

药物相关性颌骨坏死是患者使用抗骨吸收或抗血管生成药物导致的并发症,破坏了口腔卫生,影响患者饮食、语音功能,降低生活质量。对MRONJ患者最恰当的治疗策略还未达成共识。本文就近年来药物相关性颌骨坏死的分期及辅助治疗方法的研究进展作一综述。     

青少年骨性Ⅰ类错 畸形拔牙矫治前后面部软组织三维特征变化研究

目的应用3dMD^?面部三维成像系统研究拔牙矫治对青少年骨性Ⅰ类错 畸形面部软组织三维特征的影响,为正畸矫治提供参考。 方法选取2016—2019年期间于中山大学附属口腔医院正畸科就诊的青少年骨性Ⅰ类患者50例,其中拔牙组25例,非拔牙组25例为对照组,应用3dMD^?收集正畸治疗前后的面部三维图像数据,通过3dMD^? vultus软件测量线距、角度、体积变化,应用SPSS 20.0统计分析软件对各项测量指标的比较采用独立样本t检验。 结果正畸治疗后,在线距方面,拔牙组、非拔牙组前下面高变化量分别为（0.28 ± 2.28）、（1.85 ± 2.18）mm,差异有统计学意义（t = -2.238,P = 0.031）;拔牙组下颌高、颏高、唇宽变化量分别为（-0.52 ± 2.31）、（-1.14 ± 2.64）、（-1.33 ± 3.62）mm,非拔牙组则分别为（1.64 ± 2.42）、（0.93 ± 2.51）、（1.40 ± 2.16）mm,差异有统计学意义（t_下颌高 = -2.878,P_下颌高 = 0.007;t_颏高 = -2.506,P_颏高 = 0.017;t_唇宽 = -2.942,P_唇宽 = 0.006）。在角度方面,拔牙组、非拔牙组鼻唇角变化量分别为4.91° ± 3.12°、1.50° ± 4.26°,差异有统计学意义（t = 2.692,P = 0.011）;拔牙组、非拔牙组颏唇角变化量分别为-0.24° ± 6.83°、4.82° ± 6.50°,差异有统计学意义（t = -2.373,P = 0.023）;拔牙组、非拔牙组唇角变化量分别为-16.80° ± 12.65°、-1.04° ± 11.24°,差异有统计学意义（t = -4.171,P<0.001）。在体积方面,拔牙组、非拔牙组唇部变化量分别为（-0.66 ± 1.24）、（0.46 ± 1.43）cm³,差异有统计学意义（t = -2.584,P = 0.014）;拔牙组、非拔牙组颏部变化量分别为（-0.16 ± 0.94）、（0.65 ± 0.91）cm³,差异有统计学意义（t = -2.766,P = 0.009）。 结论（1）拔牙与非拔牙矫治对患者面下1/3水平向无明显影响,但对面下1/3的垂直向影响有差异,非拔牙较拔牙矫治更易导致面部高度的增加;（2）开唇露齿的青少年患者经拔牙矫治可有效改善颏、唇部形态;（3）对于面部消瘦、颧部凸出的患者,应慎重拔牙矫治。     

牙龈卟啉单胞菌诱导的耐受对小鼠牙槽骨吸收的影响

目的:研究牙龈卟啉单胞菌(P.gingivalis)诱导的耐受对小鼠骨吸收相关因子白细胞介素-1β(IL-1β)、骨保护素(OPG)/NF-κB受体激活蛋白配体(RANKL)表达水平,以及牙槽骨吸收的影响。方法:采用1×10~9CFU P.gingivalis口内涂布给菌5 d,磷酸盐缓冲液(PBS)洗脱后,再次口内涂布P.gingivalis给菌2 d,构建Balb/c小鼠牙周组织耐受模型。10 d后收集新鲜牙龈组织,采用western bolt检测牙龈组织中IL-1β、OPG和RANKL表达水平的变化。6周后,收集上颌骨,采用亚甲基蓝染色法检测牙槽骨吸收水平的改变。结果:耐受组小鼠牙龈组织中IL-1β表达水平较非耐受组降低,OPG/RANKL比值较非耐受组升高。亚甲基蓝染色结果显示,耐受组小鼠釉牙骨质界至牙槽嵴顶(ABC-CEJ)的距离小于非耐受组(P<0.01),说明耐受组小鼠牙槽骨吸收较非耐受组降低。结论:P.gingivalis诱导小鼠牙周组织耐受后,小鼠牙龈组织中骨吸收相关因子IL-1β分泌降低,OPG/RANKL比值升高,抑制了牙槽骨吸收。